FastQCFastQC Report
Thu 26 May 2016
SRR936260_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936260_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374367
Sequences flagged as poor quality0
Sequence length125
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75542.0178060566235807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74711.9956352990514656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63551.6975321008529065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.6600474935023654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21820.5828505183416273No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.5761725793138818No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT20330.5430500017362642No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.40762139825358534No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG14650.39132722702588635No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA14300.38197811238704266No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT12070.32241089625955277No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT12040.3216095435762233No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG11950.31920548552623496No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG10490.2802063216042012No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC10340.27619955818755393No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT9920.26498062062094146No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA9360.25002203719879157No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG9020.24094004012105769No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8570.22891974987111577No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC8510.22731704450445686No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT8290.22144045816004082No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT8160.21796792986561314No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7940.2120913435211971No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC7920.21155710839897748No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA7810.20861881522676948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7680.2051462869323418No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA7150.19098905619352133No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7100.18965346838797223No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT7040.18805076302131332No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6770.18083858887134818No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6660.17790029569914015No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG6470.1728250620380536No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC6320.16881829862140627No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT6140.16401018252142952No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC6120.16347594739920984No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC6010.16053765422700186No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG5870.15679800837146435No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT5860.15653089081035457No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA5760.15385971519925634No Hit
GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT5750.15359259763814653No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC5510.14718177617151085No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC5310.14183942494931442No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5310.14183942494931442No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG5210.13916824933821623No Hit
CAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTAC5120.13676419128822787No Hit
GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT5120.13676419128822787No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA5060.13516148592156893No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTC5030.13436013323823948No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5020.13409301567712967No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG4970.13275742787158057No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG4930.13168895762714128No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT4880.13035336982159218No Hit
GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA4850.12955201713826273No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA4770.12741507664938417No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT4710.12581237128272524No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG4660.12447678347717614No Hit
GAGTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCA4590.1226069605494074No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC4590.1226069605494074No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT4550.12153849030496812No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG4480.11966866737719939No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC4460.11913443225497974No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG4460.11913443225497974No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA4420.11806596201054047No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA4420.11806596201054047No Hit
GGCTTATATTATGGATCATATACATTTATAGAAACCTGAAACATTGGAGT4410.11779884444943065No Hit
CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA4340.11592902152166189No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC4340.11592902152166189No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA4330.11566190396055206No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA4330.11566190396055206No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT4180.11165514054390477No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG4160.11112090542168514No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT4100.10951820005502622No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4080.10898396493280657No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA4030.10764837712725747No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT4030.10764837712725747No Hit
ATGTAATGGTAGCTGTTGGTGGGCTAATATTTATTAATACTAGAGTAGCT4020.10738125956614765No Hit
GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG4010.10711414200503784No Hit
GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT3980.10631278932170837No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA3970.10604567176059855No Hit
ATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATT3910.10444296639393964No Hit
ACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCT3900.10417584883282982No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG3860.10310737858839054No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG3820.10203890834395125No Hit
CCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGTCTAGTAAT3810.10177179078284145No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC3810.10177179078284145No Hit
GCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTA3760.10043620297729235No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC3760.10043620297729235No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA3760.10043620297729235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCA454.0381565E-1092.606494
CACGCAG604.3655746E-1179.3775
CCAGCAG250.002051278571.439293
GGTATCA20350.069.653451
TACCTGG950.068.9326552
TGGGTAT2000.068.416946
CATGGGT2350.068.399324
TATGGTG701.72804E-1068.037432
AAGGTGT359.149711E-567.991996
GTATGGT657.39783E-964.13791
GCTTACT4450.062.835298
ATGGGTA2050.060.9847645
ACCTGGG1100.059.532743
GGATACA150.00423795359.4929920-21
CATCGTA150.00423795359.4929998-99
GGGTATG2100.059.4929857
GGGATGC2400.057.0141147
ATATAGG5050.056.585582
CTTACTA5100.055.99349
CCTTTAG655.435613E-754.9753461
Produced by FastQC (version 0.11.5)