FastQCFastQC Report
Thu 26 May 2016
SRR936259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523518
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42050.276005928384174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.060.0352671
GTACATG138500.054.123991
TACATGG140200.052.0690732
ACATGGG141450.049.716193
CATGGGA105950.046.944924
GTATCAA37000.044.2195472
ATGGGAT42050.041.8802265
ATGGGAG37300.041.7903255
CATGGGG36600.040.151264
TCAACGC42650.038.2221265
CAACGCA43450.037.3814546
ATCAACG43750.037.1251224
AACGCAG44450.036.674327
CCGAACG650.001939835436.612559
GAGTACA83150.033.485181
TATCAAC51150.032.6846583
AGTACAT82400.032.2748072
GTCGGGA3700.030.5516872
TGGGATA14000.029.322736
ACGCAGA55100.028.8298858
Produced by FastQC (version 0.11.5)