##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936259_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523518 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.975635338735742 31.0 30.0 33.0 22.0 34.0 2 28.85745032221477 31.0 30.0 34.0 20.0 34.0 3 29.11741771347631 31.0 30.0 34.0 22.0 34.0 4 32.439396186983025 35.0 33.0 37.0 20.0 37.0 5 32.78488603350929 35.0 33.0 37.0 26.0 37.0 6 32.638478836482406 35.0 33.0 37.0 24.0 37.0 7 32.45689909800869 35.0 33.0 37.0 23.0 37.0 8 32.41279787964435 35.0 33.0 37.0 22.0 37.0 9 33.60546773979697 38.0 34.0 39.0 17.0 39.0 10-11 33.57064603109382 38.0 33.5 39.0 17.5 39.0 12-13 33.594691693829674 38.0 34.0 39.0 17.5 39.0 14-15 34.61390347865926 39.0 34.0 41.0 18.0 41.0 16-17 34.600505868654 39.0 34.0 41.0 18.5 41.0 18-19 34.599710669647486 39.0 34.0 41.0 19.0 41.0 20-21 34.739369341222094 39.0 34.0 41.0 21.5 41.0 22-23 34.624075002723956 39.0 34.0 41.0 22.5 41.0 24-25 34.6052373519709 39.0 33.5 41.0 23.5 41.0 26-27 34.727915915663615 39.0 34.0 41.0 24.0 41.0 28-29 34.62158208829827 39.0 33.5 40.5 24.0 41.0 30-31 34.543503916593046 39.0 33.0 40.0 24.0 41.0 32-33 34.38870659880618 38.0 33.0 40.0 23.5 41.0 34-35 34.33348047085758 38.0 33.0 40.0 23.5 41.0 36-37 34.20111544464851 38.0 33.0 40.0 23.0 41.0 38-39 33.94251298639071 38.0 33.0 40.0 21.5 41.0 40-41 34.040358236660154 38.0 33.0 40.0 22.5 41.0 42-43 33.911293138643586 38.0 33.0 40.0 20.5 41.0 44-45 33.73068385145433 38.0 33.0 40.0 19.5 41.0 46-47 33.68200933628614 38.0 32.0 40.0 20.0 41.0 48-49 33.568484586332424 38.0 32.0 40.0 19.0 41.0 50-51 33.418583502131256 38.0 32.0 40.0 18.0 41.0 52-53 33.25063602793009 38.0 32.0 40.0 17.0 41.0 54-55 33.066731407177336 38.0 31.0 40.0 15.0 41.0 56-57 32.85922680270269 37.0 31.0 40.0 13.5 41.0 58-59 32.625368062602476 37.0 31.0 40.0 11.0 41.0 60-61 32.37616227704562 37.0 31.0 40.0 8.0 41.0 62-63 32.12901324434631 36.0 31.0 40.0 7.5 41.0 64-65 31.84725123037601 36.0 30.0 39.0 7.0 41.0 66-67 31.556736776329522 35.5 30.0 39.0 2.0 40.5 68-69 31.27272273776877 35.0 30.0 39.0 2.0 40.0 70-71 30.929487869523037 35.0 30.0 38.0 2.0 40.0 72-73 30.607748973100417 35.0 29.5 37.5 2.0 40.0 74-75 30.27227279231358 34.5 29.0 37.0 2.0 39.0 76-77 29.937867160086064 34.0 29.0 36.5 2.0 39.0 78-79 29.60067094711057 34.0 29.0 36.0 2.0 39.0 80-81 29.280659631195697 34.0 29.0 36.0 2.0 37.5 82-83 28.95755941183498 34.0 29.0 35.0 2.0 37.0 84-85 28.99290556462083 34.0 29.0 35.0 2.0 37.0 86-87 28.868404245962303 34.0 29.0 35.0 2.0 36.5 88-89 28.68001034447903 34.0 29.0 35.0 2.0 36.0 90-91 28.495400119985455 34.0 29.0 35.0 2.0 36.0 92-93 28.351686688309556 34.0 29.0 35.0 2.0 35.5 94-95 28.21253408230162 34.0 29.0 35.0 2.0 35.0 96-97 28.08753523095887 34.0 28.0 35.0 2.0 35.0 98-99 27.956272587524403 34.0 28.0 35.0 2.0 35.0 100-101 27.828389293726758 34.0 27.5 35.0 2.0 35.0 102-103 26.806194610106346 32.5 25.5 34.0 2.0 35.0 104-105 27.477442012500017 33.5 27.0 35.0 2.0 35.0 106-107 27.595206292278792 34.0 27.0 35.0 2.0 35.0 108-109 27.532343890915634 34.0 27.0 35.0 2.0 35.0 110-111 27.394363571680806 34.0 27.0 35.0 2.0 35.0 112-113 27.32692524801151 34.0 27.0 35.0 2.0 35.0 114-115 27.186696186064097 34.0 26.5 35.0 2.0 35.0 116-117 27.03578100160287 34.0 25.5 35.0 2.0 35.0 118-119 26.802450972026584 34.0 25.0 35.0 2.0 35.0 120-121 26.55647422610038 34.0 25.0 35.0 2.0 35.0 122-123 26.209519021107724 34.0 25.0 35.0 2.0 35.0 124-125 24.165214982691374 31.5 13.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.79357380674697 1101 2 0.7501785049471295 1101 3 0.6658603921375601 1101 4 0.7397402144516576 1101 5 0.5945901166481704 1101 6 0.5826476376140661 1101 7 0.5829588904797021 1101 8 0.5568668508725807 1101 9 0.6112310556471598 1101 10-11 0.7585107221807945 1101 12-13 0.625598000362352 1101 14-15 0.870899472783293 1101 16-17 0.8781460289952747 1101 18-19 0.9100702195528783 1101 20-21 0.7996292182544593 1101 22-23 0.8726612521774939 1101 24-25 0.8747485161912394 1101 26-27 0.8072130897821737 1101 28-29 0.858109733672471 1101 30-31 0.9230922027347646 1101 32-33 0.9757379732121336 1101 34-35 0.8845680729370713 1101 36-37 0.8674140103765993 1101 38-39 1.028275190842777 1101 40-41 0.8879391766448563 1101 42-43 1.046549334894621 1101 44-45 1.081249854545277 1101 46-47 0.916518313888325 1101 48-49 0.8971106716357511 1101 50-51 1.0018576063783229 1101 52-53 1.0006879598294987 1101 54-55 0.9256106223913463 1101 56-57 1.002066365060486 1101 58-59 0.9080754153632 1101 60-61 1.0378382136709874 1101 62-63 1.1452677099042887 1101 64-65 1.0643003841270335 1101 66-67 1.0117252808370907 1101 68-69 0.9137247425670658 1101 70-71 0.9796179280668653 1101 72-73 0.9927191094778465 1101 74-75 0.9668525582415342 1101 76-77 0.8725387904358151 1101 78-79 0.9334715813805872 1101 80-81 0.9985302437202286 1101 82-83 0.879871663860559 1101 84-85 0.6798078507445133 1101 86-87 0.9158971027922895 1101 88-89 0.8754787678668947 1101 90-91 0.70322270833055 1101 92-93 0.7152973470999875 1101 94-95 0.8841027509571511 1101 96-97 0.8584941992509485 1101 98-99 0.8303286292073757 1101 100-101 0.9530261383543568 1101 102-103 1.1102651196845308 1101 104-105 1.0897322206422722 1101 106-107 0.9615981234700222 1101 108-109 0.9315637295550729 1101 110-111 0.9914557661571521 1101 112-113 0.9962968253011653 1101 114-115 1.0867002925620355 1101 116-117 1.1154144792626361 1101 118-119 0.979314576934442 1101 120-121 0.9414365526481063 1101 122-123 1.0047290054509546 1101 124-125 0.9320986213102138 1102 1 0.6317218083298748 1102 2 0.6635144764548464 1102 3 0.687229604642507 1102 4 0.6368514135019154 1102 5 0.5415628115433009 1102 6 0.5323904156116868 1102 7 0.5793973550622127 1102 8 0.43735755130788334 1102 9 0.6484293144520663 1102 10-11 0.8989935080929428 1102 12-13 0.7945730696144295 1102 14-15 0.8819798051932679 1102 16-17 0.8654827919553085 1102 18-19 0.7699831597982296 1102 20-21 0.7382916638421193 1102 22-23 0.8443668319163109 1102 24-25 1.0274988129858542 1102 26-27 0.8347160577283645 1102 28-29 0.9495224760547458 1102 30-31 0.8255457049112636 1102 32-33 0.9674277239046631 1102 34-35 0.9294829918132095 1102 36-37 0.9764365667675747 1102 38-39 0.9877132596991274 1102 40-41 0.9108912937798053 1102 42-43 0.9776929834897885 1102 44-45 1.0030939384392212 1102 46-47 0.8900046613358867 1102 48-49 1.0399678144928899 1102 50-51 0.8172513539050286 1102 52-53 0.8817722495010472 1102 54-55 1.001787907709911 1102 56-57 0.9555685415543564 1102 58-59 0.9504181142155943 1102 60-61 1.1094646481783101 1102 62-63 1.119743372745603 1102 64-65 1.0484713378270776 1102 66-67 1.1090739155818454 1102 68-69 1.0795340025591464 1102 70-71 1.0967528706865721 1102 72-73 1.0291259159677573 1102 74-75 0.9909918538489713 1102 76-77 0.9932352684730148 1102 78-79 0.9696805247917482 1102 80-81 0.9203743276141729 1102 82-83 0.966139966195346 1102 84-85 0.9827362243100062 1102 86-87 0.9331111906434977 1102 88-89 0.9773782534228879 1102 90-91 0.9624233414924035 1102 92-93 1.0645256810928636 1102 94-95 1.0900782159353852 1102 96-97 1.0205440607467935 1102 98-99 0.9967314784357093 1102 100-101 1.042064523791634 1102 102-103 1.0327027928147245 1102 104-105 0.9745759088100598 1102 106-107 0.9344908816813451 1102 108-109 0.8033484545253948 1102 110-111 0.9059789161373679 1102 112-113 0.8205944113716086 1102 114-115 0.9545277559573648 1102 116-117 1.033894890817841 1102 118-119 1.0598448499854882 1102 120-121 1.087459504765242 1102 122-123 1.0983182416994701 1102 124-125 1.1311488785322155 1103 1 0.41026096231901477 1103 2 0.4399784849468702 1103 3 0.4275880783408219 1103 4 0.3998418734428384 1103 5 0.44020382420923454 1103 6 0.38761280436184364 1103 7 0.4619376856116233 1103 8 0.4538267009118613 1103 9 0.5345768228432206 1103 10-11 0.5790413819246041 1103 12-13 0.4655368331746246 1103 14-15 0.7110368025968796 1103 16-17 0.6385805924183501 1103 18-19 0.6820581800956376 1103 20-21 0.4636194374632936 1103 22-23 0.6217724531295161 1103 24-25 0.6970615284501847 1103 26-27 0.6070808852324845 1103 28-29 0.6631788637199421 1103 30-31 0.6099749503579943 1103 32-33 0.7246937838968677 1103 34-35 0.6838747676822763 1103 36-37 0.6505258062649446 1103 38-39 0.7291967082188293 1103 40-41 0.8914243243939879 1103 42-43 0.9438941344078984 1103 44-45 0.9227230969117812 1103 46-47 0.7927378026012519 1103 48-49 0.8611735225217885 1103 50-51 0.893432759720227 1103 52-53 0.7953737553206963 1103 54-55 0.7924131914893096 1103 56-57 0.6681850585017202 1103 58-59 0.7005087255213809 1103 60-61 0.7303156448392798 1103 62-63 0.617290330162966 1103 64-65 0.7429160684171201 1103 66-67 0.7172252246557065 1103 68-69 0.7363946532267747 1103 70-71 0.7701631791231804 1103 72-73 0.8412632033085288 1103 74-75 0.7479706676816065 1103 76-77 0.730163111762284 1103 78-79 0.7423079547159688 1103 80-81 0.6195899593837702 1103 82-83 0.6473101206775205 1103 84-85 0.6415538731577968 1103 86-87 0.6275729579023164 1103 88-89 0.7160167367378101 1103 90-91 0.7644923537887607 1103 92-93 0.734725964170984 1103 94-95 0.9663510333835958 1103 96-97 0.9016411873501653 1103 98-99 0.8056834633978021 1103 100-101 0.8452598579948152 1103 102-103 0.840738284237041 1103 104-105 0.9578373421184736 1103 106-107 0.8044331107337293 1103 108-109 0.861561246163447 1103 110-111 0.7485197633336611 1103 112-113 0.8881437729402748 1103 114-115 0.9536240201090891 1103 116-117 0.9035264185759608 1103 118-119 0.8663455356519734 1103 120-121 0.8516477613815283 1103 122-123 0.909147429839603 1103 124-125 0.9783576144922179 1104 1 0.4923689969110221 1104 2 0.5727905326714691 1104 3 0.5147589804530597 1104 4 0.6962886987421086 1104 5 0.6102864183717287 1104 6 0.5510964302574735 1104 7 0.41186476642543113 1104 8 0.34254892996295894 1104 9 0.5963028544772229 1104 10-11 0.671589667590176 1104 12-13 0.6399696773401047 1104 14-15 0.7987495826854598 1104 16-17 0.7873818073416103 1104 18-19 0.7213086073410082 1104 20-21 0.7730809298680441 1104 22-23 0.8219029860065916 1104 24-25 0.8337007430324377 1104 26-27 0.6933324266861902 1104 28-29 0.8089284166488042 1104 30-31 0.8233996937133838 1104 32-33 1.0258671523350387 1104 34-35 0.9173566885615898 1104 36-37 0.9751217372236809 1104 38-39 1.2010461751038477 1104 40-41 1.0072316496198965 1104 42-43 0.9781445814790288 1104 44-45 1.1123299518704073 1104 46-47 1.025651516880174 1104 48-49 1.0409856535146247 1104 50-51 1.0128216501511176 1104 52-53 1.0487494893698681 1104 54-55 1.1277203521613188 1104 56-57 1.07575081067165 1104 58-59 1.0638828338573205 1104 60-61 1.017102006294806 1104 62-63 0.9115420460834613 1104 64-65 0.9960364465342231 1104 66-67 1.028732102215713 1104 68-69 0.9417170004394713 1104 70-71 1.124819023562246 1104 72-73 0.9176779108585791 1104 74-75 0.9271681204665327 1104 76-77 1.0622150394220107 1104 78-79 0.9320501800398198 1104 80-81 0.8270130453046995 1104 82-83 0.9042358573040445 1104 84-85 0.9497875159053315 1104 86-87 0.9940767624677953 1104 88-89 0.9277381291413676 1104 90-91 0.9850970398080214 1104 92-93 0.9653835056960602 1104 94-95 1.0678750007583062 1104 96-97 0.9793717709812135 1104 98-99 1.0231460819063471 1104 100-101 1.0044560419303927 1104 102-103 1.0117038389422923 1104 104-105 0.9706869002020362 1104 106-107 1.0231310333894292 1104 108-109 1.0023281862935818 1104 110-111 1.0356244473703455 1104 112-113 0.9938794478293502 1104 114-115 1.140276755867248 1104 116-117 1.1675046578095767 1104 118-119 1.0887381097111444 1104 120-121 1.0668701470562212 1104 122-123 1.0958797065915071 1104 124-125 1.2298942307089078 1105 1 0.45479477819126046 1105 2 0.563713205861383 1105 3 0.553362933107902 1105 4 0.5245657406761737 1105 5 0.5160858130101786 1105 6 0.454749036331215 1105 7 0.48647581001610973 1105 8 0.33496209309318914 1105 9 0.591392304000756 1105 10-11 0.5813695079667056 1105 12-13 0.6435919379242563 1105 14-15 0.6540372068349001 1105 16-17 0.6139883765208083 1105 18-19 0.5822389443152787 1105 20-21 0.4868980363636837 1105 22-23 0.40923343445409444 1105 24-25 0.6293447718606515 1105 26-27 0.610088168718562 1105 28-29 0.755816455726368 1105 30-31 0.7930943809303486 1105 32-33 0.8021919419680259 1105 34-35 0.8806531221924487 1105 36-37 0.8817177009639181 1105 38-39 0.9950170217584855 1105 40-41 0.8404180034390194 1105 42-43 1.0021556823545694 1105 44-45 0.952087497601525 1105 46-47 0.7607518040785592 1105 48-49 0.9240234537762788 1105 50-51 0.798337175120345 1105 52-53 0.8916152028372295 1105 54-55 0.8357671709166965 1105 56-57 0.9041089803431888 1105 58-59 0.9113346642631939 1105 60-61 0.8973234819216529 1105 62-63 0.9934648044265373 1105 64-65 1.0471697576761265 1105 66-67 0.9174702098028575 1105 68-69 0.857901055123282 1105 70-71 0.6921637158109881 1105 72-73 0.7902855328404819 1105 74-75 0.6888618168103662 1105 76-77 0.7004495821882486 1105 78-79 0.7534012510343331 1105 80-81 0.7708149790555403 1105 82-83 0.7574894821192224 1105 84-85 0.7313797251653504 1105 86-87 0.8387835646570032 1105 88-89 0.7553455575933583 1105 90-91 0.6963397340539892 1105 92-93 0.6924290781188205 1105 94-95 0.677540426253163 1105 96-97 0.6391447216930182 1105 98-99 0.7067386937085764 1105 100-101 0.6746493396026132 1105 102-103 0.6906764273661032 1105 104-105 0.6919160473544395 1105 106-107 0.747565964763723 1105 108-109 0.7513276366686448 1105 110-111 0.7150086352027323 1105 112-113 0.6743109421124736 1105 114-115 0.6964153697286335 1105 116-117 0.7958564797001308 1105 118-119 0.7294852125684201 1105 120-121 0.7668246288218299 1105 122-123 0.7041384793022409 1105 124-125 0.6852355787608317 1106 1 -0.18801020984082228 1106 2 -0.05882094016412864 1106 3 -0.03358177570047616 1106 4 -0.011930175093326056 1106 5 -0.16079442997284588 1106 6 -0.20300069374064122 1106 7 -0.28123290697827485 1106 8 -0.3946997559445897 1106 9 -0.24596848954029582 1106 10-11 -0.15556655452554224 1106 12-13 -0.24001372359740003 1106 14-15 -0.4054683715774985 1106 16-17 -0.25103239771526376 1106 18-19 -0.30289259199970786 1106 20-21 -0.4222357966097974 1106 22-23 -0.42637363386319294 1106 24-25 -4.115208433599513 1106 26-27 -1.1568401490129432 1106 28-29 -0.9276912193407654 1106 30-31 -0.7581100416757351 1106 32-33 -5.398537210179136 1106 34-35 -1.1510630884810453 1106 36-37 -0.46542112225707655 1106 38-39 -0.15323883781302783 1106 40-41 -0.6840775332464446 1106 42-43 -0.31076903715555204 1106 44-45 -0.31557844935932877 1106 46-47 -0.43272582734775966 1106 48-49 -0.1396820496193243 1106 50-51 -0.05040113966033033 1106 52-53 0.03985067741075454 1106 54-55 -0.32322427476890425 1106 56-57 -0.20114991836229024 1106 58-59 0.024409094275576138 1106 60-61 -0.06565943868086066 1106 62-63 4.8262845338342686E-4 1106 64-65 0.15995764384875955 1106 66-67 0.09511939029516725 1106 68-69 0.11314892285248845 1106 70-71 0.09475788757852044 1106 72-73 0.12174436945249312 1106 74-75 0.17677874386653514 1106 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2203 58-59 0.8996968927775271 2203 60-61 0.9147652754835391 2203 62-63 0.9561341463400908 2203 64-65 0.9300645222772026 2203 66-67 0.90324616032634 2203 68-69 1.0698197019606432 2203 70-71 0.9441015863238391 2203 72-73 0.9628129059607069 2203 74-75 0.9216850120575728 2203 76-77 0.7719735459349693 2203 78-79 0.7680561870182743 2203 80-81 0.8654445924659591 2203 82-83 0.8381886434248464 2203 84-85 0.8546015991125024 2203 86-87 0.8124005564170993 2203 88-89 0.8526549462523505 2203 90-91 0.8297246800045812 2203 92-93 0.852536019802745 2203 94-95 0.9151778512395943 2203 96-97 0.9496604621561389 2203 98-99 0.9064712116392712 2203 100-101 0.7051113854696496 2203 102-103 0.7730316888164026 2203 104-105 0.807997927298203 2203 106-107 0.9242960795751607 2203 108-109 0.8402106049524605 2203 110-111 0.8409241891188479 2203 112-113 1.0006071017885318 2203 114-115 0.9887293517400835 2203 116-117 0.9224961978466517 2203 118-119 0.9964544054617335 2203 120-121 1.0807059509146129 2203 122-123 0.963354751724804 2203 124-125 0.8878727448317818 2204 1 0.5892757801003548 2204 2 0.6049764825439894 2204 3 0.5044803490656911 2204 4 0.5688446768068758 2204 5 0.6035998994995779 2204 6 0.6551338295921241 2204 7 0.5764751305455462 2204 8 0.465841118212623 2204 9 0.6755459858388164 2204 10-11 0.8385527956210339 2204 12-13 0.8339789637312975 2204 14-15 0.8127588690766601 2204 16-17 0.6902425868116282 2204 18-19 0.7457345917896703 2204 20-21 0.5963787486367025 2204 22-23 0.7927776593770091 2204 24-25 0.8576542397036917 2204 26-27 0.7590632640871107 2204 28-29 0.8542080988020402 2204 30-31 0.7525460356603588 2204 32-33 0.9107170117703163 2204 34-35 0.9119484561083908 2204 36-37 0.9739138719437577 2204 38-39 0.958672078663362 2204 40-41 0.8878199992037565 2204 42-43 1.0117755921571927 2204 44-45 0.9888023920659634 2204 46-47 0.8578158963137099 2204 48-49 0.861373762810139 2204 50-51 0.8423714861919933 2204 52-53 0.8374242158733551 2204 54-55 0.8847239644168212 2204 56-57 0.8902515382774538 2204 58-59 0.9095595865546215 2204 60-61 0.9219456007864153 2204 62-63 0.9805262132226389 2204 64-65 0.8242136254855943 2204 66-67 0.9639212598980009 2204 68-69 0.8098828390497594 2204 70-71 0.7287134394355554 2204 72-73 0.648632045919662 2204 74-75 0.6672738314781625 2204 76-77 0.5847886624230796 2204 78-79 0.6702214509113986 2204 80-81 0.5198702957874595 2204 82-83 0.6046589392598172 2204 84-85 0.5700680902182675 2204 86-87 0.4493591012743643 2204 88-89 0.5872622313313229 2204 90-91 0.5990938756149511 2204 92-93 0.5176654916038999 2204 94-95 0.662386275674347 2204 96-97 0.589266337530379 2204 98-99 0.6589073320401688 2204 100-101 0.704090454710876 2204 102-103 0.6316874232038003 2204 104-105 0.7555946508890017 2204 106-107 0.6055945044061346 2204 108-109 0.706375022695287 2204 110-111 0.7857644569660209 2204 112-113 0.8184601525958612 2204 114-115 0.7043248611183017 2204 116-117 0.7331219331935053 2204 118-119 0.8399138175903005 2204 120-121 0.7228932559750412 2204 122-123 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0.46845012688828547 2205 58-59 0.5630650273507314 2205 60-61 0.6442184183014135 2205 62-63 0.5191882796471319 2205 64-65 0.49376601242625995 2205 66-67 0.5844622550117435 2205 68-69 0.6939823292866016 2205 70-71 0.6871893921460028 2205 72-73 0.5325774738964917 2205 74-75 0.5383090859638138 2205 76-77 0.4993519930686574 2205 78-79 0.6052745743428289 2205 80-81 0.5981690451921402 2205 82-83 0.49563570374423094 2205 84-85 0.6099162327965075 2205 86-87 0.564868479885579 2205 88-89 0.5949494909596424 2205 90-91 0.5444512846113057 2205 92-93 0.727259025769385 2205 94-95 0.6605127322537463 2205 96-97 0.5738445020545626 2205 98-99 0.6503246414188162 2205 100-101 0.7043603685384454 2205 102-103 0.6184861980551801 2205 104-105 0.6793132634473764 2205 106-107 0.6744926104728357 2205 108-109 0.6858341739203624 2205 110-111 0.7179395022619488 2205 112-113 0.7499208205415293 2205 114-115 0.7306216222661917 2205 116-117 0.617601416571933 2205 118-119 0.5907321361539815 2205 120-121 0.5482308799626416 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0.24756193129367432 2207 54-55 0.15244895608967113 2207 56-57 0.15182918549942315 2207 58-59 0.30112866038172115 2207 60-61 0.25492138532008823 2207 62-63 0.1480687691441176 2207 64-65 0.10957661123030604 2207 66-67 0.1969644230206029 2207 68-69 0.2425930297899761 2207 70-71 0.29511188780012887 2207 72-73 0.2969572853979763 2207 74-75 0.3158257993412157 2207 76-77 0.22828091785451576 2207 78-79 0.19380792620278697 2207 80-81 0.16965667374521232 2207 82-83 0.21109685893818764 2207 84-85 0.18383819039025084 2207 86-87 0.18919812169109562 2207 88-89 0.3126791717893198 2207 90-91 0.37045526980578103 2207 92-93 0.2903813428584172 2207 94-95 0.35626520871113954 2207 96-97 0.35432202571542604 2207 98-99 0.29797097216236423 2207 100-101 0.30695572150999695 2207 102-103 0.30945649042103085 2207 104-105 0.32336513404483824 2207 106-107 0.3458786382470045 2207 108-109 0.20773968618143712 2207 110-111 0.22020285424015995 2207 112-113 0.26506436243731457 2207 114-115 0.30381828234999375 2207 116-117 0.3479232870511417 2207 118-119 0.21216108064901462 2207 120-121 0.22371616903706126 2207 122-123 0.21782642068039237 2207 124-125 0.4028440208335482 2208 1 0.23400106334347015 2208 2 0.0641233841229969 2208 3 0.18478822955793817 2208 4 0.09473210844810609 2208 5 0.17019934345031373 2208 6 0.08370397114738637 2208 7 0.10711733412702529 2208 8 0.051747941544931564 2208 9 0.29552136546244867 2208 10-11 0.5521570834149472 2208 12-13 0.45270556128502903 2208 14-15 0.3003309674792902 2208 16-17 0.3024410619937328 2208 18-19 -0.22512611168267682 2208 20-21 -0.3605760487330443 2208 22-23 -0.18170003303895044 2208 24-25 -0.061563507501269044 2208 26-27 0.03379982402015003 2208 28-29 0.2767720516065282 2208 30-31 -0.12767882500435945 2208 32-33 0.13056104532365964 2208 34-35 -0.27803708171526154 2208 36-37 0.18628153147135862 2208 38-39 0.4123354050455177 2208 40-41 0.4558524870856431 2208 42-43 0.5106914388812385 2208 44-45 0.42709217681955636 2208 46-47 0.3527425757313907 2208 48-49 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0.2016739140331829 2208 114-115 0.15821503017006222 2208 116-117 0.09710735746833521 2208 118-119 0.14662057537706374 2208 120-121 0.3045711506030955 2208 122-123 0.3503559122765907 2208 124-125 0.23657639043591416 2209 1 0.2067384816354405 2209 2 0.12259234975597622 2209 3 0.15297419241906596 2209 4 0.15246825409467846 2209 5 0.10873406709047373 2209 6 0.23894643107303892 2209 7 0.1580713986207769 2209 8 0.07516951162573804 2209 9 0.1965529074891137 2209 10-11 0.33551018237641017 2209 12-13 0.22291265285676332 2209 14-15 0.23999568835039042 2209 16-17 0.23836301666517556 2209 18-19 0.22716576883969708 2209 20-21 0.19740343790138581 2209 22-23 0.31143852028489505 2209 24-25 -1.808203933431912 2209 26-27 -0.08999011396985424 2209 28-29 0.34387997193612563 2209 30-31 0.31000840071259006 2209 32-33 -2.5798547880632867 2209 34-35 -0.40829968020543106 2209 36-37 0.17388827285582664 2209 38-39 0.3569256831534986 2209 40-41 0.33102730101369815 2209 42-43 0.44173503607638764 2209 44-45 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82-83 -4.364008800837336 2216 84-85 -4.5712134599622765 2216 86-87 -4.456867796053768 2216 88-89 -4.293186607069423 2216 90-91 -4.342535566368408 2216 92-93 -4.350100057033927 2216 94-95 -4.296740446412695 2216 96-97 -4.434245038338322 2216 98-99 -4.288307045952639 2216 100-101 -4.455923224949071 2216 102-103 -4.245388990463855 2216 104-105 -4.379924440192781 2216 106-107 -4.352550752448291 2216 108-109 -4.625965366881033 2216 110-111 -4.637007345583076 2216 112-113 -4.7007374224819145 2216 114-115 -4.766623644645083 2216 116-117 -4.627132034812458 2216 118-119 -4.625287750780451 2216 120-121 -4.771402465625993 2216 122-123 -5.01835972659206 2216 124-125 -4.833626211684411 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59368.0 3 10968.0 4 7947.0 5 6397.0 6 6194.0 7 6128.0 8 6379.0 9 7049.0 10 8293.0 11 9380.0 12 9945.0 13 10094.0 14 10309.0 15 9684.0 16 9420.0 17 8236.0 18 7349.0 19 6931.0 20 6754.0 21 6899.0 22 7988.0 23 11273.0 24 17206.0 25 23795.0 26 25834.0 27 24459.0 28 29116.0 29 34536.0 30 41242.0 31 50865.0 32 64373.0 33 83456.0 34 112534.0 35 160344.0 36 245585.0 37 295261.0 38 81782.0 39 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.511843015554824 18.518700845545233 9.157250934353081 21.812205204546856 2 18.711967112936712 22.017804855475852 36.88340858684215 22.386819444745285 3 17.224673420333726 29.977131875041845 29.7009946715431 23.09720003308133 4 10.66133251067433 17.368782059907517 35.87263663304923 36.09724879636892 5 10.503041986569182 45.11769196284109 33.283054733441375 11.096211317148349 6 30.19499656714674 38.23489643022644 18.652601813964296 12.917505188662526 7 27.90987396874719 36.04844336535805 20.72731998653111 15.314362679363654 8 26.815092050956217 36.0923240109642 19.190432056627355 17.90215188145223 9 27.443559423828606 15.99404005894303 17.95209073813345 38.61030977909491 10-11 23.404346095238907 29.440852034994297 28.096124365812926 19.058677503953874 12-13 24.124968616723635 28.36365545839275 27.568284266025163 19.943091658858453 14-15 22.07102747301435 29.438462220092614 24.751377589030486 23.739132717862546 16-17 21.94444034204191 31.467363746904738 25.315729103592226 21.27246680746112 18-19 21.292402050152397 30.041979092173825 27.176005983604163 21.489612874069618 20-21 23.10538246471022 28.98578169188119 26.681866381445012 21.22696946196357 22-23 22.73060648430523 29.429783029895756 26.452918646803386 21.38669183899563 24-25 22.13153921890384 29.186708237610766 27.014112241549064 21.66764030193633 26-27 22.078259645272038 29.129924375067894 26.79110774460611 22.00070823505396 28-29 22.008840135844558 29.134443842032788 26.955981794623717 21.900734227498937 30-31 22.157138553169034 28.77990875012881 27.166429604288524 21.89652309241363 32-33 21.644445961415265 29.140192608618026 27.063105840220963 22.152255589745746 34-35 22.100190382797088 29.173431475156537 27.049716460579997 21.676661681466378 36-37 22.345249206768038 28.760090159985403 26.797029761459363 22.097630871787196 38-39 21.969421240511622 28.90551644444386 27.011500099933805 22.11356221511071 40-41 22.042760551805802 28.968787147023285 27.20949099675945 21.778961304411467 42-43 21.913391132090517 28.818490526283313 27.154038599820346 22.114079741805817 44-45 21.842068125802015 28.891044007075788 27.302010823725553 21.96487704339664 46-47 22.032269032441004 28.923947689011648 26.75647717367332 22.287306104874027 48-49 21.862728849520927 28.815455024310516 27.10001690167655 22.221799224492006 50-51 22.07783683375369 28.76228046136957 27.026942762014627 22.132939942862112 52-53 22.140553951982515 28.625753401279614 27.097175432349363 22.13651721438851 54-55 21.8570079707674 28.768441445364946 27.220037748493688 22.154512835373968 56-57 22.057496289810285 29.011484686319132 26.997545794013416 21.933473229857164 58-59 22.36914911562715 28.4578889917946 26.918154077199414 22.25480781537884 60-61 22.045615806163017 28.645093196158598 27.269877985463804 22.03941301221458 62-63 21.689583660840082 28.88122162998427 27.1104370807982 22.31875762837745 64-65 21.712908067135825 28.952677323428126 27.061220859614505 22.273193749821544 66-67 21.83126995999059 29.14271030212013 26.94106036921819 22.084959368671097 68-69 21.954478981513816 28.694710131038992 27.184617686981955 22.16619320046524 70-71 21.665183800471738 28.735551079790234 27.30107084709128 22.298194272646747 72-73 21.867666021339446 28.849342950995275 27.047097947413107 22.23589308025217 74-75 21.703148116787897 29.030090960765705 27.1107124712999 22.156048451146496 76-77 21.573940246036464 28.936458739466786 27.26876812421809 22.220832890278658 78-79 21.67861108075369 28.995372872806346 27.136448562551546 22.189567483888414 80-81 21.725786003349494 28.95584518558898 27.066923222588297 22.25144558847323 82-83 21.732215656274263 28.970631768467303 27.07436149383302 22.222791081425413 84-85 21.521310416598975 28.807288143770116 27.288531032339346 22.38287040729156 86-87 21.52391016826221 28.80867598940608 27.308034381020207 22.35937946131151 88-89 21.66757957838903 28.710280545594113 27.286072620951508 22.33606725506535 90-91 21.561039870588864 28.836576433533867 27.193601119515282 22.40878257636199 92-93 21.535509355345603 28.850768683451545 27.247009216849992 22.366712744352856 94-95 21.31894949542686 28.878012727799618 27.32929202229974 22.473745754473782 96-97 21.53353315639595 28.84458927040077 27.253531226647333 22.36834634655594 98-99 21.357019341122555 28.905810278923976 27.035300920797983 22.701869459155485 100-101 21.119502519172755 28.92860987347688 27.30315927105459 22.64872833629578 102-103 21.293959768547555 28.67310640334044 27.372425311427335 22.66050851668467 104-105 21.162593632098385 28.825427497971795 27.31343156225468 22.698547307675142 106-107 21.05756131628547 29.173795195254133 27.251201088010653 22.51744240044975 108-109 21.114733589233296 29.238365040774212 26.9199061116683 22.726995258324187 110-111 21.00660218186735 29.251469385035822 27.143739971769165 22.598188461327663 112-113 21.104663298138384 29.163860653725532 27.084490198317834 22.64698584981825 114-115 20.765145326808508 29.40467583341018 27.039913305715174 22.790265534066133 116-117 20.749813342522625 29.269566216227304 27.214807886380974 22.765812554869093 118-119 20.754573320818373 29.39586875045126 26.966413085572132 22.883144843158234 120-121 20.74796186577933 29.29655693894409 27.13366176276088 22.821819432515696 122-123 20.773954415238197 29.17095910210182 26.947677194852552 23.107409287807425 124-125 20.844257902200535 29.24308942048834 26.719616571016182 23.193036106294947 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3329.0 1 3151.5 2 2528.0 3 1758.5 4 1218.5 5 914.0 6 802.5 7 773.5 8 745.0 9 731.0 10 686.0 11 646.0 12 687.5 13 739.0 14 802.0 15 896.5 16 1029.5 17 1199.5 18 1444.0 19 1707.5 20 2078.0 21 2851.0 22 4053.5 23 5627.0 24 7207.5 25 9047.5 26 11384.5 27 13892.5 28 16426.5 29 19350.0 30 22873.5 31 26105.0 32 29268.0 33 32361.5 34 35343.0 35 37394.0 36 39758.0 37 42606.5 38 43609.5 39 44025.0 40 45167.5 41 47851.0 42 49951.0 43 51692.5 44 54311.5 45 56335.5 46 57003.5 47 56323.0 48 55148.0 49 53303.5 50 52129.0 51 50246.5 52 47218.0 53 45122.5 54 43697.0 55 41370.5 56 37415.5 57 33341.0 58 30690.5 59 27980.5 60 23581.5 61 19666.5 62 16813.0 63 13249.5 64 9776.5 65 7727.5 66 6033.5 67 4780.5 68 3713.0 69 2573.5 70 1997.0 71 1624.0 72 1442.0 73 1292.5 74 1061.0 75 831.0 76 525.0 77 345.5 78 261.0 79 171.0 80 93.0 81 65.0 82 53.0 83 34.0 84 25.0 85 20.5 86 17.5 87 15.0 88 9.0 89 5.5 90 6.0 91 4.0 92 3.5 93 4.0 94 3.0 95 2.0 96 1.5 97 2.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.625502291407125E-4 2 6.563755728517812E-5 3 0.0 4 1.9691267185553436E-4 5 4.594629009962469E-4 6 2.625502291407125E-4 7 4.594629009962469E-4 8 9.189258019924938E-4 9 8.532882447073156E-4 10-11 8.204694660647265E-4 12-13 0.0010173821379202608 14-15 0.0015096638175590969 16-17 0.0013455699243461515 18-19 0.0014112074816313295 20-21 0.0016081201534868638 22-23 0.07951990065099329 24-25 0.0011814760311332061 26-27 8.204694660647265E-4 28-29 7.876506874221374E-4 30-31 5.907380155666031E-4 32-33 9.189258019924938E-4 34-35 5.57919236924014E-4 36-37 2.625502291407125E-4 38-39 6.235567942091922E-4 40-41 7.220131301369593E-4 42-43 8.861070233499047E-4 44-45 8.532882447073156E-4 46-47 9.189258019924938E-4 48-49 3.281877864258906E-5 50-51 6.891943514943702E-4 52-53 6.891943514943702E-4 54-55 8.861070233499047E-4 56-57 7.220131301369593E-4 58-59 5.907380155666031E-4 60-61 7.220131301369593E-4 62-63 8.204694660647265E-4 64-65 6.235567942091922E-4 66-67 9.845633592776718E-5 68-69 1.3127511457035625E-4 70-71 2.9536900778330153E-4 72-73 9.845633592776718E-5 74-75 2.625502291407125E-4 76-77 2.625502291407125E-4 78-79 1.640938932129453E-4 80-81 2.2973145049812345E-4 82-83 6.563755728517812E-5 84-85 1.640938932129453E-4 86-87 1.9691267185553436E-4 88-89 2.9536900778330153E-4 90-91 9.845633592776718E-5 92-93 2.625502291407125E-4 94-95 1.640938932129453E-4 96-97 2.2973145049812345E-4 98-99 1.640938932129453E-4 100-101 1.3127511457035625E-4 102-103 5.57919236924014E-4 104-105 1.3127511457035625E-4 106-107 2.2973145049812345E-4 108-109 3.938253437110687E-4 110-111 4.266441223536578E-4 112-113 2.625502291407125E-4 114-115 2.2973145049812345E-4 116-117 3.6100656506847967E-4 118-119 5.25100458281425E-4 120-121 2.9536900778330153E-4 122-123 2.9536900778330153E-4 124-125 1.640938932129453E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1523518.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.308179549961 #Duplication Level Percentage of deduplicated Percentage of total 1 55.19854949036964 19.489602963033796 2 13.708808626029983 9.680661527678417 3 9.703524684742982 10.278413755091519 4 6.607292068442364 9.331658187663848 5 4.408401024887831 7.782630745748581 6 2.8832702611800416 6.108181444368473 7 1.9231557064618443 4.753218889040083 8 1.238675837387129 3.4988311096530436 9 0.8770540169793289 2.7870462635868622 >10 3.2527730247683033 18.574836855434786 >50 0.13643043849628475 3.271119461851752 >100 0.0598147735256082 3.6675544161471016 >500 0.0018750389404823262 0.4309894173449082 >1k 3.7500778809646527E-4 0.34525496335694256 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4205 0.276005928384174 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.563755728517812E-5 0.0 16-17 0.0 0.0 0.0 6.563755728517812E-5 0.0 18-19 0.0 0.0 0.0 1.3127511457035625E-4 0.0 20-21 0.0 0.0 0.0 1.9691267185553436E-4 0.0 22-23 0.0 0.0 0.0 2.9536900778330153E-4 0.0 24-25 0.0 0.0 0.0 6.235567942091922E-4 0.0 26-27 0.0 0.0 0.0 7.548319087795484E-4 0.0 28-29 0.0 0.0 0.0 0.0013455699243461513 0.0 30-31 0.0 0.0 0.0 0.0042992600021791675 0.0 32-33 0.0 0.0 0.0 0.01010818382191743 0.0 34-35 0.0 0.0 0.0 0.017558046573785145 0.0 36-37 0.0 0.0 0.0 0.03176857772602621 0.0 38-39 0.0 0.0 0.0 0.05484017911176632 0.0 40-41 0.0 0.0 0.0 0.08358942920267434 0.0 42-43 0.0 0.0 0.0 0.11630975150933563 0.0 44-45 0.0 0.0 0.0 0.15582356099501285 0.0 46-47 0.0 0.0 0.0 0.20419844071418913 0.0 48-49 0.0 0.0 0.0 0.25615056730540764 0.0 50-51 0.0 0.0 0.0 0.31158148443274053 0.0 52-53 0.0 0.0 0.0 0.3703927357602601 0.0 54-55 0.0 0.0 0.0 0.4399357277039063 0.0 56-57 0.0 0.0 0.0 0.5323862271400797 0.0 58-59 0.0 0.0 0.0 0.6352074606273113 0.0 60-61 0.0 0.0 0.0 0.7440345306061367 0.0 62-63 0.0 0.0 0.0 0.8521724062334675 0.0 64-65 0.0 0.0 0.0 0.9734706120964767 0.0 66-67 0.0 0.0 0.0 1.1249620943106677 0.0 68-69 0.0 0.0 0.0 1.3096333617325167 0.0 70-71 0.0 0.0 0.0 1.4963722122088483 0.0 72-73 0.0 0.0 0.0 1.6798948223782062 0.0 74-75 0.0 0.0 0.0 1.8824195053816233 0.0 76-77 0.0 0.0 0.0 2.1253112861154246 0.0 78-79 0.0 0.0 0.0 2.409784459389387 0.0 80-81 0.0 0.0 0.0 2.7061380305319664 0.0 82-83 0.0 0.0 0.0 3.0106306587779073 0.0 84-85 0.0 0.0 0.0 3.332714152376276 0.0 86-87 0.0 0.0 0.0 3.6928346104214063 0.0 88-89 0.0 0.0 0.0 4.092600153066783 0.0 90-91 0.0 0.0 0.0 4.531715411304625 0.0 92-93 0.0 0.0 0.0 4.967680066792779 0.0 94-95 0.0 0.0 0.0 5.421596594198428 0.0 96-97 0.0 0.0 0.0 5.916438138571385 0.0 98-99 0.0 0.0 0.0 6.459359193655736 0.0 100-101 0.0 0.0 0.0 7.055906133042077 0.0 102-103 0.0 0.0 0.0 7.6473333429601755 0.0 104-105 0.0 0.0 0.0 8.253233634259654 0.0 106-107 0.0 0.0 0.0 8.883715190762434 0.0 108-109 0.0 0.0 0.0 9.553316731407179 0.0 110-111 0.0 0.0 0.0 10.271129057877886 0.0 112-113 0.0 0.0 0.0 10.982377628620075 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2745 0.0 60.035267 1 GTACATG 13850 0.0 54.12399 1 TACATGG 14020 0.0 52.069073 2 ACATGGG 14145 0.0 49.71619 3 CATGGGA 10595 0.0 46.94492 4 GTATCAA 3700 0.0 44.219547 2 ATGGGAT 4205 0.0 41.880226 5 ATGGGAG 3730 0.0 41.790325 5 CATGGGG 3660 0.0 40.15126 4 TCAACGC 4265 0.0 38.222126 5 CAACGCA 4345 0.0 37.381454 6 ATCAACG 4375 0.0 37.125122 4 AACGCAG 4445 0.0 36.67432 7 CCGAACG 65 0.0019398354 36.61255 9 GAGTACA 8315 0.0 33.48518 1 TATCAAC 5115 0.0 32.684658 3 AGTACAT 8240 0.0 32.274807 2 GTCGGGA 370 0.0 30.551687 2 TGGGATA 1400 0.0 29.32273 6 ACGCAGA 5510 0.0 28.829885 8 >>END_MODULE