FastQCFastQC Report
Thu 26 May 2016
SRR936259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523518
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95910.6295298119221433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77040.5056717413250122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71280.4678645083287496No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.127861961591527No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.12444880861269772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.12254531945142756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.083.608211
GTATCAA77650.054.2034152
GTACATG118100.046.2590031
TACATGG122250.044.4114232
ACATGGG122950.043.868053
CATGGGA89400.041.1528244
ATCAACG112500.038.4178244
TCAACGC112550.038.400765
ATGGGAG31700.038.122865
CAACGCA113950.037.85316
TATCAAC117200.037.181953
AACGCAG116700.037.012097
CATGGGG33300.034.860944
ATGGGAT34450.034.3884435
ACGCAGA135800.031.7625988
CGCAGAG137250.031.383699
ATAACGC2307.2759576E-1231.0600473
ATGGGAC17750.030.8558965
GAGTACA80250.029.9925351
AGTACAT78550.029.9364032
Produced by FastQC (version 0.11.5)