##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936259_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523518 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486394647125927 31.0 31.0 34.0 30.0 34.0 2 31.240112686558348 31.0 31.0 34.0 28.0 34.0 3 31.60469190386986 31.0 31.0 34.0 30.0 34.0 4 35.47011784567035 37.0 35.0 37.0 33.0 37.0 5 35.135928817381874 37.0 35.0 37.0 33.0 37.0 6 35.36841179428139 37.0 35.0 37.0 33.0 37.0 7 35.18573328309872 37.0 35.0 37.0 32.0 37.0 8 35.28638978994669 37.0 35.0 37.0 32.0 37.0 9 36.89808784668117 39.0 37.0 39.0 33.0 39.0 10-11 36.81355750309481 39.0 37.0 39.0 32.0 39.0 12-13 36.76321349665708 39.0 37.0 39.0 32.0 39.0 14-15 37.87497161175647 40.0 37.0 41.0 32.0 41.0 16-17 37.79930857397156 40.0 37.0 41.0 32.0 41.0 18-19 37.941170041968654 40.0 37.0 41.0 32.0 41.0 20-21 38.00018673885047 40.0 37.0 41.0 33.0 41.0 22-23 37.898488235780604 40.0 37.0 41.0 32.0 41.0 24-25 37.85187999091576 40.0 37.0 41.0 32.0 41.0 26-27 37.66795961714925 40.0 37.0 41.0 32.0 41.0 28-29 37.514429104218 40.0 36.0 41.0 32.0 41.0 30-31 37.299106410295124 39.0 36.0 41.0 31.5 41.0 32-33 37.03390737753016 39.0 35.5 41.0 31.0 41.0 34-35 36.802412245867785 39.0 35.0 41.0 30.5 41.0 36-37 36.56537697618276 39.0 35.0 41.0 30.0 41.0 38-39 36.52344212539661 39.0 35.0 41.0 30.0 41.0 40-41 36.51768242974484 39.0 35.0 41.0 30.0 41.0 42-43 36.37645600511448 39.0 35.0 41.0 30.0 41.0 44-45 36.15034216858612 39.0 35.0 41.0 30.0 41.0 46-47 35.79858196621241 39.0 35.0 41.0 28.0 41.0 48-49 35.45988429411402 39.0 35.0 41.0 26.5 41.0 50-51 35.26164246172346 39.0 35.0 41.0 26.0 41.0 52-53 35.11307644543747 39.0 35.0 41.0 26.0 41.0 54-55 34.93814283782666 39.0 34.5 41.0 25.0 41.0 56-57 34.742405078246534 38.0 34.0 40.0 24.5 41.0 58-59 34.52735379562303 38.0 34.0 40.0 24.0 41.0 60-61 34.30548999092889 38.0 34.0 40.0 23.5 41.0 62-63 34.091055373156074 37.5 34.0 40.0 23.0 41.0 64-65 33.84924529936634 37.0 33.0 40.0 23.0 41.0 66-67 33.573480589005186 36.5 33.0 40.0 22.5 41.0 68-69 33.269376206910586 36.0 33.0 39.0 22.0 41.0 70-71 32.94624677883688 36.0 33.0 39.0 22.0 41.0 72-73 32.685054590756394 35.0 32.5 39.0 22.0 40.0 74-75 32.496885826094605 35.0 33.0 37.5 22.0 40.0 76-77 32.1942251420725 35.0 32.0 37.0 21.5 39.0 78-79 31.861210697871634 35.0 32.0 37.0 20.0 39.0 80-81 31.57588292360182 35.0 32.0 36.0 20.0 39.0 82-83 31.315060931344426 35.0 32.0 36.0 20.0 37.0 84-85 31.05640104022401 35.0 32.0 35.5 19.0 37.0 86-87 30.84820625683451 35.0 32.0 35.0 18.0 36.5 88-89 30.679622426515472 35.0 32.0 35.0 18.0 36.0 90-91 30.53073084794535 35.0 31.0 35.0 18.0 36.0 92-93 30.39008039288016 34.5 31.0 35.0 17.0 36.0 94-95 30.263929274219272 34.0 31.0 35.0 15.0 35.5 96-97 30.192169045590536 34.0 31.0 35.0 12.5 35.0 98-99 30.09978024545821 34.0 31.0 35.0 10.5 35.0 100-101 30.013511491167154 34.0 31.0 35.0 8.5 35.0 102-103 28.99665117182731 33.5 29.5 34.5 7.0 35.0 104-105 29.74062269037845 34.0 31.0 35.0 7.0 35.0 106-107 29.881366022587194 34.0 31.0 35.0 6.0 35.0 108-109 29.84694306204456 34.0 31.0 35.0 2.0 35.0 110-111 29.806114532286458 34.0 31.0 35.0 2.0 35.0 112-113 29.723001959937463 34.0 31.0 35.0 2.0 35.0 114-115 29.691694157863576 34.0 31.0 35.0 2.0 35.0 116-117 29.580608827726355 34.0 31.0 35.0 2.0 35.0 118-119 29.441331510359575 34.0 31.0 35.0 2.0 35.0 120-121 29.24288127872464 34.0 31.0 35.0 2.0 35.0 122-123 29.009016959432053 34.0 31.0 35.0 2.0 35.0 124-125 26.96008416047595 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27074228518156573 1101 2 0.6837980858210919 1101 3 0.47165009353010134 1101 4 0.35166915804658316 1101 5 0.6545915018773485 1101 6 0.4006679231175667 1101 7 0.5554065940648698 1101 8 0.490119945730342 1101 9 0.4478610218265615 1101 10-11 0.4628941923170373 1101 12-13 0.4747992838990953 1101 14-15 0.529843892931595 1101 16-17 0.634400979313483 1101 18-19 0.6141989724055819 1101 20-21 0.501356671334733 1101 22-23 0.4667185117950865 1101 24-25 0.34756061164094376 1101 26-27 0.39102018841899877 1101 28-29 0.43610181828825745 1101 30-31 0.2907299938989638 1101 32-33 0.31989629273834197 1101 34-35 0.37489420294225795 1101 36-37 0.4698789569353323 1101 38-39 0.41735795881756843 1101 40-41 0.4048122497418021 1101 42-43 0.4847057515831992 1101 44-45 0.46527224059925487 1101 46-47 0.4754224665839004 1101 48-49 0.3929550787401652 1101 50-51 0.3021765886082193 1101 52-53 0.33910518545044965 1101 54-55 0.34539797055214194 1101 56-57 0.1474629029275505 1101 58-59 0.3970504111223008 1101 60-61 0.4300023270628728 1101 62-63 0.32878333595888165 1101 64-65 0.2881403458534635 1101 66-67 0.47179461894672414 1101 68-69 0.46905486757178494 1101 70-71 0.3439625724733091 1101 72-73 0.5109901176599934 1101 74-75 0.48892670837585683 1101 76-77 0.5574286977781853 1101 78-79 0.5629685184961204 1101 80-81 0.5860784737778175 1101 82-83 0.4218610043437714 1101 84-85 0.44253349154840294 1101 86-87 0.4519189375757904 1101 88-89 0.4106348524882897 1101 90-91 0.4500421247687534 1101 92-93 0.4840643696839848 1101 94-95 0.4482728721459708 1101 96-97 0.44086331625155495 1101 98-99 0.39447360368676243 1101 100-101 0.37921316840119346 1101 102-103 0.41676958329444247 1101 104-105 0.39564378466209504 1101 106-107 0.4377902651725911 1101 108-109 0.35790106592595095 1101 110-111 0.4456302896174478 1101 112-113 0.46048144894829335 1101 114-115 0.42320906332447095 1101 116-117 0.4694344753823856 1101 118-119 0.548974681678132 1101 120-121 0.6242438160153903 1101 122-123 0.5689237075630054 1101 124-125 0.5264731756204029 1102 1 0.2929029500015119 1102 2 0.7000228582785546 1102 3 0.477981712050088 1102 4 0.29824612678422113 1102 5 0.5208360606426794 1102 6 0.3658440212576508 1102 7 0.4679511132575911 1102 8 0.45806612697292337 1102 9 0.4727917697490014 1102 10-11 0.47674460782950945 1102 12-13 0.4848903009153247 1102 14-15 0.5935558042889326 1102 16-17 0.6506257517709457 1102 18-19 0.5938190752943839 1102 20-21 0.5334104900921517 1102 22-23 0.5767303835798216 1102 24-25 0.4989258668843135 1102 26-27 0.46165730753257606 1102 28-29 0.5595683794279509 1102 30-31 0.4171645012199008 1102 32-33 0.5567383980014995 1102 34-35 0.3974505939197073 1102 36-37 0.3719367329543246 1102 38-39 0.5736697910296726 1102 40-41 0.45586092405917356 1102 42-43 0.33155972863107763 1102 44-45 0.41006844162814104 1102 46-47 0.27102990623566114 1102 48-49 0.45508408546751156 1102 50-51 0.35540175679184216 1102 52-53 0.34049022700170184 1102 54-55 0.4061419357282432 1102 56-57 0.4044870422231597 1102 58-59 0.3895316141298224 1102 60-61 0.40744593608543056 1102 62-63 0.2985102849102077 1102 64-65 0.3211834800046347 1102 66-67 0.2856054618434385 1102 68-69 0.34657762182583696 1102 70-71 0.22939984987733908 1102 72-73 0.2620783645931155 1102 74-75 0.26375852475892714 1102 76-77 0.18030166968163286 1102 78-79 0.24045170013442885 1102 80-81 0.31718254975724136 1102 82-83 0.4264118551550098 1102 84-85 0.30086352716375586 1102 86-87 0.17827429966522246 1102 88-89 0.2018893044075618 1102 90-91 0.3004576372341283 1102 92-93 0.2994581172106976 1102 94-95 0.30561359236757824 1102 96-97 0.2511126237307799 1102 98-99 0.3109753844544727 1102 100-101 0.44391439515228015 1102 102-103 0.473754149974301 1102 104-105 0.4817142239181287 1102 106-107 0.358051444436537 1102 108-109 0.25026355108622056 1102 110-111 0.24420567545045202 1102 112-113 0.2651905902225309 1102 114-115 0.30132540681477593 1102 116-117 0.48466993244609924 1102 118-119 0.5006960904632507 1102 120-121 0.34070602416734985 1102 122-123 0.3597824333247779 1102 124-125 0.43189462397813827 1103 1 0.22795679142166136 1103 2 0.6647209788091004 1103 3 0.4675622439079419 1103 4 0.261987182731211 1103 5 0.43330577697342676 1103 6 0.2590207799450468 1103 7 0.3232757220203979 1103 8 0.36061083475760825 1103 9 0.4738383758940188 1103 10-11 0.47304527349573533 1103 12-13 0.4352134350157755 1103 14-15 0.5080881461462496 1103 16-17 0.5982535263905504 1103 18-19 0.5340803528163249 1103 20-21 0.42679834499170966 1103 22-23 0.47401535665158434 1103 24-25 0.3113799570239806 1103 26-27 0.3810950213922979 1103 28-29 0.3889001822609188 1103 30-31 0.1597349684477649 1103 32-33 0.22787030952049037 1103 34-35 0.229119035305402 1103 36-37 0.006831966950201718 1103 38-39 0.18235945883682092 1103 40-41 0.0479879386848765 1103 42-43 0.07718189905830997 1103 44-45 0.21596737630543572 1103 46-47 0.17163354314447332 1103 48-49 0.18003681465469157 1103 50-51 0.11883620024507024 1103 52-53 0.06767483903871607 1103 54-55 0.14676499285464217 1103 56-57 0.11545504355566294 1103 58-59 0.19810284935486777 1103 60-61 0.14969492533552398 1103 62-63 0.039117969414562026 1103 64-65 0.13219919985776585 1103 66-67 0.06820235413317732 1103 68-69 0.08762556554746936 1103 70-71 0.24197196054944925 1103 72-73 0.3384065234807352 1103 74-75 0.2785542772875971 1103 76-77 0.24804786420488512 1103 78-79 0.20401525776487972 1103 80-81 0.2555444368115758 1103 82-83 0.19869340108293798 1103 84-85 0.16699920972957472 1103 86-87 0.25519327437789485 1103 88-89 0.2514801945600311 1103 90-91 0.24797756577837404 1103 92-93 0.16387856381231103 1103 94-95 0.18943508040886314 1103 96-97 0.2603151190401469 1103 98-99 0.3061051159137804 1103 100-101 0.28075516917806453 1103 102-103 0.43377780479459105 1103 104-105 0.2809865283645081 1103 106-107 0.287152594546388 1103 108-109 0.24346029418229165 1103 110-111 0.26345671496459033 1103 112-113 0.19505054563558843 1103 114-115 0.23269219615073666 1103 116-117 0.3168436893516926 1103 118-119 0.37006612516575643 1103 120-121 0.23682957187057596 1103 122-123 0.25734633232253046 1103 124-125 0.2610333973918699 1104 1 0.2792482818041826 1104 2 0.5585799858551823 1104 3 0.3655588100843872 1104 4 0.25765104867727473 1104 5 0.508253536066519 1104 6 0.2816529286224707 1104 7 0.4379509957151697 1104 8 0.33032102163532073 1104 9 0.4687155552419142 1104 10-11 0.3648835586458361 1104 12-13 0.36170764390802645 1104 14-15 0.436488801189725 1104 16-17 0.5256152317240819 1104 18-19 0.4785803270428275 1104 20-21 0.4350154926976728 1104 22-23 0.36284313810813984 1104 24-25 0.3851357114540477 1104 26-27 0.24194340579020945 1104 28-29 0.31465330382857815 1104 30-31 0.1713743614619574 1104 32-33 0.1926279394685082 1104 34-35 0.3076862664778588 1104 36-37 0.22395873689590928 1104 38-39 0.25254498840299533 1104 40-41 0.33521514598710667 1104 42-43 0.25130411304182587 1104 44-45 0.2475292902060744 1104 46-47 0.18709082455543324 1104 48-49 0.1973601769468658 1104 50-51 0.16517854373052643 1104 52-53 0.29805537488965683 1104 54-55 0.2543224182007009 1104 56-57 0.24874544748592342 1104 58-59 0.19697722137357232 1104 60-61 0.30362800146252056 1104 62-63 0.21701318624901234 1104 64-65 0.11952291901382495 1104 66-67 0.1963683513496406 1104 68-69 0.23318875622776147 1104 70-71 0.2778326567512224 1104 72-73 0.25031840189324583 1104 74-75 0.31107060381012275 1104 76-77 0.2706689897535668 1104 78-79 0.2628812236566205 1104 80-81 0.23468986677307413 1104 82-83 0.2079666279650958 1104 84-85 0.17894249216354297 1104 86-87 0.2766787004535374 1104 88-89 0.22275606223490385 1104 90-91 0.19158522169815484 1104 92-93 0.10076393516882831 1104 94-95 0.17435028558576704 1104 96-97 0.22766651497269308 1104 98-99 0.130469481865795 1104 100-101 0.15710492750191563 1104 102-103 0.15666316822080262 1104 104-105 0.13353687591027708 1104 106-107 0.20593172356061373 1104 108-109 0.24279192778617897 1104 110-111 0.33233953276178596 1104 112-113 0.4174377270850087 1104 114-115 0.2909920173222815 1104 116-117 0.2876649917462508 1104 118-119 0.27329851888971035 1104 120-121 0.3239171264410139 1104 122-123 0.26479146450094504 1104 124-125 0.290768802650458 1105 1 0.20197627938892637 1105 2 0.6105859620587424 1105 3 0.28210654617152287 1105 4 0.2035588200642664 1105 5 0.38946333022811075 1105 6 0.31737994307528083 1105 7 0.40916675059792595 1105 8 0.17723112312241085 1105 9 0.30470453177834855 1105 10-11 0.11550997625241877 1105 12-13 0.2563176811431802 1105 14-15 0.19967770960363396 1105 16-17 0.33547689654576374 1105 18-19 0.29305930582420814 1105 20-21 0.2795731994456787 1105 22-23 0.28295089556097963 1105 24-25 0.19222618281549586 1105 26-27 0.2804817131002224 1105 28-29 0.25546899368529097 1105 30-31 0.2852691121567261 1105 32-33 0.20573099801517003 1105 34-35 0.21768177658399424 1105 36-37 0.2737110960254938 1105 38-39 0.28869010486928914 1105 40-41 0.19042860947575235 1105 42-43 0.22991944525526264 1105 44-45 0.3209855795410306 1105 46-47 0.31702904980876667 1105 48-49 0.11201116511057307 1105 50-51 0.16193459541075583 1105 52-53 0.17836904816472554 1105 54-55 0.28997163363045786 1105 56-57 0.23446619156971593 1105 58-59 0.2941827269123962 1105 60-61 0.25534445692354524 1105 62-63 0.2194808474013712 1105 64-65 0.255601074131917 1105 66-67 0.29343087699235326 1105 68-69 0.32253803126472746 1105 70-71 0.25987718411248295 1105 72-73 0.25582564378240846 1105 74-75 0.31372862098009335 1105 76-77 0.31321762381947593 1105 78-79 0.2718881829921216 1105 80-81 0.3742808219592888 1105 82-83 0.3456456624830899 1105 84-85 0.34638131735958666 1105 86-87 0.34733128330369567 1105 88-89 0.3296560227548042 1105 90-91 0.2479729472572636 1105 92-93 0.31739930159835694 1105 94-95 0.19930697359233562 1105 96-97 0.21382649453593316 1105 98-99 0.3234068788111202 1105 100-101 0.22968669388809104 1105 102-103 0.1066307326082061 1105 104-105 0.18266846850659846 1105 106-107 0.21862047327999434 1105 108-109 0.1939385537857632 1105 110-111 0.28541410370598186 1105 112-113 0.27913808085768466 1105 114-115 0.3853453564873206 1105 116-117 0.35919333613659177 1105 118-119 0.3172607728164323 1105 120-121 0.3511929220424648 1105 122-123 0.3142230284548404 1105 124-125 0.3919461611994457 1106 1 0.24660080926472006 1106 2 0.5980955737776092 1106 3 0.2977671478012738 1106 4 0.20793444065284206 1106 5 0.37537998897155234 1106 6 0.2501720662505136 1106 7 0.31063197809911713 1106 8 0.24078739294778018 1106 9 0.11625776400824606 1106 10-11 0.3178087732452184 1106 12-13 0.17645010516714166 1106 14-15 0.1896436751186883 1106 16-17 0.26458125819685563 1106 18-19 0.20375409117248466 1106 20-21 0.1269294113622692 1106 22-23 0.10131010168761634 1106 24-25 0.16509705823808218 1106 26-27 0.2656491270116774 1106 28-29 0.2177242083216271 1106 30-31 0.28073818096078895 1106 32-33 0.08395302002851679 1106 34-35 0.2154688888959413 1106 36-37 0.22613235685530952 1106 38-39 0.13993538978079556 1106 40-41 0.2289479478387193 1106 42-43 0.2465609114489382 1106 44-45 0.27483802107916233 1106 46-47 0.1484888864395444 1106 48-49 0.17105027322921984 1106 50-51 0.08688357137614844 1106 52-53 0.15199593777711584 1106 54-55 0.207759398262354 1106 56-57 0.2221701146279642 1106 58-59 0.152773995856208 1106 60-61 0.16304687841515175 1106 62-63 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-0.5915915920457024 2216 86-87 -0.5041502787482806 2216 88-89 -0.5148257175036441 2216 90-91 -0.408282019646105 2216 92-93 -0.46720687012525275 2216 94-95 -0.5743075914955753 2216 96-97 -0.5562859521425985 2216 98-99 -0.6511952872805225 2216 100-101 -0.8710936818867445 2216 102-103 -0.7073516121073347 2216 104-105 -0.5891477765385389 2216 106-107 -0.4983984856410082 2216 108-109 -0.5899126486262887 2216 110-111 -0.6028179275427661 2216 112-113 -0.5425589183700907 2216 114-115 -0.5991255950549714 2216 116-117 -0.5815000315322578 2216 118-119 -0.6508862250466976 2216 120-121 -0.8093064895550839 2216 122-123 -0.8866040261774835 2216 124-125 -0.9013760730280787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59.0 3 45.0 4 161.0 5 407.0 6 1006.0 7 1847.0 8 3059.0 9 5024.0 10 8270.0 11 10897.0 12 11957.0 13 14756.0 14 19951.0 15 14820.0 16 7265.0 17 6259.0 18 6055.0 19 5755.0 20 5953.0 21 5818.0 22 6561.0 23 7699.0 24 9476.0 25 12216.0 26 15643.0 27 19798.0 28 25325.0 29 31719.0 30 39582.0 31 49386.0 32 62308.0 33 78554.0 34 105167.0 35 148748.0 36 242784.0 37 380672.0 38 157741.0 39 775.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.51005219694148 18.492114629659188 10.994461094657941 22.003372078741386 2 19.113091034160455 22.006660908057416 36.209006009782634 22.671242047999495 3 18.216152636401585 27.791240787756742 32.295427156656054 21.69717941918562 4 11.456707436341414 16.701410813656288 36.791951260175466 35.049930489826835 5 10.234498865730615 42.33786909730022 35.18868804373669 12.238943993232478 6 30.22845808188679 37.451608710891506 17.942026283903438 14.377906923318267 7 25.74396889304885 34.91228853219982 22.65014263041198 16.69359994433935 8 27.455862024603583 33.12819408763139 20.437500574328627 18.9784433134364 9 26.859741729339593 11.3115171596266 19.94180574171096 41.88693536932284 10-11 24.097549224886087 25.856340391121073 29.95678423228344 20.0893261517094 12-13 25.49057510314942 25.47498618329419 29.888783722935997 19.145654990620393 14-15 22.511399610243927 26.407713205694456 28.33463623970064 22.746250944360973 16-17 20.869172818822232 28.745321271334262 28.63134161570182 21.75416429414168 18-19 20.055595029266154 26.167536638076033 32.624436542409256 21.152431790248553 20-21 21.607030570035928 25.441150022513682 32.88586022613451 20.065959181315876 22-23 21.056955021207493 26.00067737959118 32.57907028338359 20.363297315817732 24-25 20.657189478562117 25.979574904923997 32.8897000232357 20.47353559327819 26-27 20.62171237884948 25.932414320014598 32.76961611218246 20.67625718895346 28-29 20.58571017867856 25.965725380336824 32.86846627345394 20.580098167530675 30-31 20.63162364999954 25.67015945988167 33.06721679691346 20.63100009320533 32-33 20.45771694197246 26.014986367079352 32.67316172175189 20.854134969196295 34-35 20.95354461441318 26.074726512464103 32.41463575526471 20.55709311785801 36-37 21.14602518644348 25.84219549752612 32.003231993320725 21.008547322709674 38-39 20.884196970432907 26.087089223757122 32.00500420736742 21.02370959844255 40-41 20.96382220749023 26.128810466568318 32.04249376853236 20.864873557409087 42-43 21.155054161932462 26.145644826637838 31.61487429823819 21.084426713191515 44-45 21.074805219698526 26.316855314782956 31.381930873588587 21.226408591929935 46-47 21.303167082196044 26.694578687347292 30.480228547721545 21.522025682735116 48-49 21.524565359380592 26.797395274339706 30.00695818850783 21.67108117777187 50-51 21.667629971481585 26.962589967254203 29.723549569827785 21.646230491436434 52-53 21.60257497967579 27.000067938818304 29.81565605759899 21.581701023906916 54-55 21.345827973978395 27.165833074322915 29.740391151132005 21.747947800566685 56-57 21.668779206048406 27.15116351579941 29.516562155590986 21.663495122561198 58-59 21.798755472703764 26.602427353344666 29.727694014322566 21.871123159629004 60-61 21.719852627944636 26.716829964863592 30.0173089306074 21.546008476584372 62-63 21.387674031948823 26.97756939781385 29.92565141165173 21.709105158585597 64-65 21.24304395028796 26.979725551922094 30.00162783706401 21.775602660725937 66-67 21.552646200975204 27.077608351687736 29.749162524003488 21.620582923333572 68-69 21.662203811417317 26.772241445118368 29.722287769326122 21.843266974138196 70-71 21.35671275533295 26.87104133211113 29.82904560720169 21.943200305354235 72-73 21.53712402066836 27.04924489067193 29.49137111680565 21.92225997185406 74-75 21.417520782668912 27.21353064151414 29.45753377901615 21.9114147968008 76-77 21.183873008871632 27.147182686274046 29.64093394980532 22.028010355049005 78-79 21.545352478952477 27.208500859688755 29.288425822759383 21.957720838599386 80-81 21.30424180684976 27.138157948715257 29.28828658229809 22.2693136621369 82-83 21.40541306904126 27.043520697019037 29.285537767395365 22.26552846654434 84-85 21.211258971036695 26.89249983918756 29.403865012070778 22.492376177704966 86-87 21.158342973884363 26.84831926223119 29.285581838022335 22.70775592586211 88-89 21.372380290371044 26.834248004101084 29.20658300399278 22.586788701535085 90-91 21.264687146267093 26.95972749294626 29.248396848406955 22.527188512379688 92-93 21.319080267684758 26.76926318428811 29.228323964060586 22.683332583966546 94-95 21.033245815556285 26.812044634066297 29.244502789629145 22.910206760748277 96-97 21.342643508066487 26.868459321645073 29.053173477160566 22.73572369312787 98-99 21.202248525914563 26.81995317321382 28.847538574336372 23.130259726535243 100-101 21.043758336046377 26.724708304014953 28.976655131853935 23.25487822808473 102-103 21.06827418897489 26.326022117434544 29.16539766223128 23.440306031359288 104-105 21.056818595785444 26.3584697538212 29.07680407008437 23.507907580308977 106-107 20.920866945408957 26.536747379406766 29.098364959862423 23.444020715321855 108-109 20.908569682596934 26.77035085882582 28.61340416126109 23.707675297316154 110-111 20.80794508984862 26.691935120744443 28.66393131111311 23.83618847829383 112-113 20.94355612680966 26.402366094814468 28.72398667676719 23.930091101608685 114-115 20.575813836233554 26.52341949740615 28.654467055930272 24.246299610430025 116-117 20.64279749587723 26.535468945306345 28.69257008707709 24.129163471739336 118-119 20.52817542828916 26.623625773989364 28.543595314953972 24.304603482767504 120-121 20.519844102564104 26.657903589743594 28.43785846153846 24.384393846153845 122-123 20.44630150869878 26.50088746987066 28.333657163562272 24.719153857868285 124-125 20.345276772471856 26.319304707194778 28.249601478754233 25.085817041579134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 178.5 2 610.5 3 1202.5 4 964.0 5 795.5 6 1280.5 7 3035.5 8 4501.0 9 4834.0 10 4075.0 11 2711.5 12 2443.5 13 2455.5 14 2621.0 15 2835.0 16 3080.0 17 3427.0 18 3810.5 19 4081.5 20 4405.5 21 5065.0 22 6013.5 23 7297.5 24 8608.0 25 10262.5 26 12639.5 27 15183.0 28 17627.0 29 20135.5 30 23231.5 31 26377.5 32 29276.0 33 31892.5 34 34697.5 35 36560.0 36 38672.5 37 41684.5 38 42977.5 39 43518.0 40 44381.0 41 46247.5 42 48167.5 43 50120.0 44 52542.5 45 54382.0 46 55392.5 47 54870.5 48 53697.0 49 51865.5 50 50923.0 51 49181.0 52 45869.5 53 43991.5 54 42560.5 55 39864.5 56 36089.0 57 32439.0 58 29470.0 59 26681.0 60 22675.5 61 19025.5 62 16347.5 63 12798.5 64 9250.0 65 7100.0 66 5365.5 67 4216.5 68 3120.5 69 2000.5 70 1600.0 71 1392.5 72 1237.5 73 1107.0 74 894.0 75 637.0 76 373.5 77 223.5 78 139.0 79 68.0 80 31.5 81 9.0 82 3.5 83 2.5 84 1.5 85 1.0 86 0.5 87 2.0 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06662212064445579 2 1.3127511457035625E-4 3 1.3127511457035625E-4 4 0.0 5 0.06248695453548957 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.563755728517812E-5 16-17 3.281877864258906E-5 18-19 3.281877864258906E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.640938932129453E-4 36-37 0.0 38-39 0.0 40-41 3.281877864258906E-5 42-43 0.0020347642758405215 44-45 0.008861070233499047 46-47 0.010502009165628498 48-49 0.00872979511892869 50-51 0.007646775423723251 52-53 0.005808923819738263 54-55 0.006005836491593798 56-57 0.004922816796388359 58-59 0.0031506027496885497 60-61 0.0017722140466998094 62-63 0.0012471135884183843 64-65 0.0015753013748442748 66-67 0.002723958627334892 68-69 0.001083019695205439 70-71 0.002723958627334892 72-73 0.0019691267185553436 74-75 8.532882447073156E-4 76-77 6.563755728517812E-4 78-79 9.845633592776718E-5 80-81 2.9536900778330153E-4 82-83 9.845633592776718E-5 84-85 2.625502291407125E-4 86-87 3.6100656506847967E-4 88-89 0.0012471135884183843 90-91 0.002264495726338645 92-93 6.235567942091922E-4 94-95 0.0011814760311332061 96-97 0.0015424825962016856 98-99 0.0020347642758405215 100-101 9.189258019924938E-4 102-103 9.189258019924938E-4 104-105 7.548319087795484E-4 106-107 5.25100458281425E-4 108-109 8.861070233499047E-4 110-111 7.220131301369593E-4 112-113 0.010764559394769212 114-115 0.019691267185553436 116-117 0.017722140466998094 118-119 0.010534827944271088 120-121 0.005283823361456839 122-123 0.005644829926525318 124-125 0.004824360460460592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1523518.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.981663865501456 #Duplication Level Percentage of deduplicated Percentage of total 1 41.14037003238176 11.511760055484556 2 17.16108172758151 9.603912409391697 3 12.584355513384548 10.563936178184893 4 8.864256317766857 9.921465628055994 5 5.899618624058222 8.254057263652467 6 4.042516229802576 6.786979818790195 7 2.6055663937595956 5.103565810659946 8 1.7268809572203374 3.8656801984539904 9 1.1940371543003234 3.0070031665096386 >10 4.479014397687553 20.251084906399353 >50 0.20366087570426075 3.9112401055939694 >100 0.09413653773148048 4.577608640067985 >500 0.002608296043748157 0.47784918514821706 >1k 0.0011855891107946167 0.543402502966384 >5k 7.1135346647677E-4 1.620454130640781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9591 0.6295298119221433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7704 0.5056717413250122 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7128 0.4678645083287496 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1948 0.127861961591527 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 0.12444880861269772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1867 0.12254531945142756 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 9.845633592776718E-5 0.0 16-17 0.0 0.0 0.0 1.3127511457035625E-4 0.0 18-19 0.0 0.0 0.0 1.9691267185553436E-4 0.0 20-21 0.0 0.0 0.0 2.9536900778330153E-4 0.0 22-23 0.0 0.0 0.0 4.266441223536578E-4 0.0 24-25 0.0 0.0 0.0 7.548319087795484E-4 0.0 26-27 0.0 0.0 0.0 8.861070233499046E-4 0.0 28-29 0.0 0.0 0.0 0.0015424825962016859 0.0 30-31 0.0 0.0 0.0 0.004561810231319879 0.0 32-33 0.0 0.0 0.0 0.010666103058841445 0.0 34-35 0.0 0.0 0.0 0.018247240925279518 0.0 36-37 0.0 0.0 0.0 0.03281877864258906 0.0 38-39 0.0 0.0 0.0 0.056317024150682835 0.0 40-41 0.0 0.0 0.0 0.08542728080665932 0.0 42-43 0.0 0.0 0.0 0.1181804218919632 0.0 44-45 0.0 0.0 0.0 0.1581208754999941 0.0 46-47 0.0 0.0 0.0 0.20659421155509813 0.0 48-49 0.0 0.0 0.0 0.25903861982595544 0.0 50-51 0.0 0.0 0.0 0.31492899985428463 0.0 52-53 0.0 0.0 0.0 0.374396626754656 0.0 54-55 0.0 0.0 0.0 0.44443190037794106 0.0 56-57 0.0 0.0 0.0 0.5376044129442514 0.0 58-59 0.0 0.0 0.0 0.6417712163558291 0.0 60-61 0.0 0.0 0.0 0.7523705003813542 0.0 62-63 0.0 0.0 0.0 0.8623790463913128 0.0 64-65 0.0 0.0 0.0 0.9854494663010218 0.0 66-67 0.0 0.0 0.0 1.1388772564551255 0.0 68-69 0.0 0.0 0.0 1.3266334890693776 0.0 70-71 0.0 0.0 0.0 1.5169167676391089 0.0 72-73 0.0 0.0 0.0 1.703721255672726 0.0 74-75 0.0 0.0 0.0 1.90887144096755 0.0 76-77 0.0 0.0 0.0 2.1547497305578274 0.0 78-79 0.0 0.0 0.0 2.4424063253601203 0.0 80-81 0.0 0.0 0.0 2.742074593145601 0.0 82-83 0.0 0.0 0.0 3.0524089639899232 0.0 84-85 0.0 0.0 0.0 3.3787917175904716 0.0 86-87 0.0 0.0 0.0 3.7438678112106323 0.0 88-89 0.0 0.0 0.0 4.150853485157379 0.0 90-91 0.0 0.0 0.0 4.5976155188189445 0.0 92-93 0.0 0.0 0.0 5.0414238624026755 0.0 94-95 0.0 0.0 0.0 5.502855890117479 0.0 96-97 0.0 0.0 0.0 6.0071492427395015 0.0 98-99 0.0 0.0 0.0 6.560572306989481 0.0 100-101 0.0 0.0 0.0 7.16988575126779 0.0 102-103 0.0 0.0 0.0 7.773127721497219 0.0 104-105 0.0 0.0 0.0 8.39021921631382 0.0 106-107 0.0 0.0 0.0 9.03340164015128 0.0 108-109 0.0 0.0 6.563755728517812E-5 9.717607537291979 0.0 110-111 0.0 0.0 6.563755728517812E-5 10.452124622091764 0.0 112-113 0.0 0.0 6.563755728517812E-5 11.180603051621315 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5045 0.0 83.60821 1 GTATCAA 7765 0.0 54.203415 2 GTACATG 11810 0.0 46.259003 1 TACATGG 12225 0.0 44.411423 2 ACATGGG 12295 0.0 43.86805 3 CATGGGA 8940 0.0 41.152824 4 ATCAACG 11250 0.0 38.417824 4 TCAACGC 11255 0.0 38.40076 5 ATGGGAG 3170 0.0 38.12286 5 CAACGCA 11395 0.0 37.8531 6 TATCAAC 11720 0.0 37.18195 3 AACGCAG 11670 0.0 37.01209 7 CATGGGG 3330 0.0 34.86094 4 ATGGGAT 3445 0.0 34.388443 5 ACGCAGA 13580 0.0 31.762598 8 CGCAGAG 13725 0.0 31.38369 9 ATAACGC 230 7.2759576E-12 31.060047 3 ATGGGAC 1775 0.0 30.855896 5 GAGTACA 8025 0.0 29.992535 1 AGTACAT 7855 0.0 29.936403 2 >>END_MODULE