FastQCFastQC Report
Thu 26 May 2016
SRR936258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562446
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.062.1572151
GTACATG288950.054.0885931
TACATGG292650.052.5514952
ACATGGG292350.050.957053
CATGGGA185050.046.3927654
GTATCAA75850.043.610622
CATGGGG115550.038.0493744
ATGGGAT83200.037.8988155
ATCAACG90650.036.4905174
TCAACGC90450.036.373885
CAACGCA91850.035.689916
ATGGGAG78700.035.6056755
AACGCAG96550.034.014547
TATCAAC109950.030.3557283
GAGTACA172250.029.8071081
ATGGGGG61350.027.6377935
ACGCAGA116600.027.6042868
AGTACAT176900.027.3423442
CGCAGAG117350.027.0222789
ATGGGGA72050.025.7628365
Produced by FastQC (version 0.11.5)