FastQCFastQC Report
Thu 26 May 2016
SRR936258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4562446
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160280.35130278802203907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126300.27682519420503826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117750.2580852463788065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93000.084.046031
GTATCAA148900.052.2952
GTACATG257450.045.1934661
ACATGGG261000.044.135453
TACATGG261700.044.0401462
CATGGGA162050.040.0060734
ATCAACG209300.038.341384
TCAACGC210900.038.1069565
CAACGCA214350.037.525716
AACGCAG219300.036.7872057
TATCAAC229100.035.41753
CATGGGG101800.034.4440844
ACGCAGA257400.031.7118286
CGCAGAG261250.031.1534067
ATGGGAT74300.031.0878055
ATGGGAG70900.030.563445
GAGTACA172400.030.2368871
AGTACAT177650.028.6850532
CAGAGTA292200.027.9146989
GCAGAGT295050.027.544248
Produced by FastQC (version 0.11.5)