FastQCFastQC Report
Thu 26 May 2016
SRR936257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410386
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51750.3669208287660257No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG16730.11862000899044658No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15420.10933177158593463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32800.060.0366741
GTACATG179000.055.836571
TACATGG181450.054.3285372
ACATGGG185800.050.7831883
CATGGGA135300.048.983514
TACCTGG11700.048.8143922
CATGGGG43850.047.485494
ATGGGAT49800.046.948935
GTCGGGA5650.045.2775042
ATGGGAG45700.045.042495
ATGGGGG25250.042.881755
GAACGCA3150.041.5518159
GTATCAA48450.040.398442
GGAACGC3200.039.0433658
TGGGATA15400.039.0178346
CGGGAGT6950.037.6643374
ATGGGAC22650.037.035095
GGGATAG7400.036.983167
TCAACGC56900.035.026365
TGGGAGT12400.034.5440526
Produced by FastQC (version 0.11.5)