FastQCFastQC Report
Thu 26 May 2016
SRR936257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410386
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149731.0616242645630345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123290.8741578546582283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115370.8180030147775148No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.2679408332187075No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30200.2141257783330237No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.1852684300609904No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.1537876864914995No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.13095705714605788No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15520.11004079734200424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69650.086.225751
ATAACGC2300.056.9447063
GTATCAA115550.051.9336782
TAACGCA2500.050.0078054
GTACATG153850.049.0268251
ACATGGG157900.047.204213
TACATGG159850.047.1497652
CATGGGA117050.045.4190984
TATAACG2650.044.9306372
TACCTGG12900.044.3037032
GTCGGGA6200.044.1697242
ATGGGAT47500.041.359845
GGAACGC3200.040.9014328
ATGGGAG36100.040.073535
CATGGGG37600.039.1081774
ATCCACG2350.037.999852
ATCAACG164250.037.912714
TCAACGC165000.037.776465
CAACGCA166950.037.416986
GGGAACG4150.037.272727