##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936257_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1410386 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.565216189043284 31.0 31.0 34.0 30.0 34.0 2 31.29057293535245 31.0 31.0 34.0 28.0 34.0 3 31.632351001782492 31.0 31.0 34.0 30.0 34.0 4 35.50327569899304 37.0 35.0 37.0 33.0 37.0 5 35.17927219924191 37.0 35.0 37.0 33.0 37.0 6 35.421600895074114 37.0 35.0 37.0 33.0 37.0 7 35.245198832092775 37.0 35.0 37.0 32.0 37.0 8 35.3431457771135 37.0 35.0 37.0 33.0 37.0 9 36.95592341387393 39.0 37.0 39.0 33.0 39.0 10-11 36.8679379262131 39.0 37.0 39.0 32.0 39.0 12-13 36.80728573596164 39.0 37.0 39.0 32.0 39.0 14-15 37.8439366953444 40.0 37.0 41.0 32.0 41.0 16-17 37.783206866772645 40.0 37.0 41.0 32.0 41.0 18-19 38.002227049899815 40.0 37.0 41.0 33.0 41.0 20-21 38.0758955349812 40.0 37.0 41.0 33.0 41.0 22-23 37.94673550361391 40.0 37.0 41.0 33.0 41.0 24-25 37.883211759050354 40.0 37.0 41.0 33.0 41.0 26-27 37.67819518911844 40.0 36.5 41.0 32.0 41.0 28-29 37.49582171121948 40.0 36.0 41.0 32.0 41.0 30-31 37.17834798416887 39.0 35.5 41.0 31.5 41.0 32-33 36.79149608688685 39.0 35.0 41.0 31.0 41.0 34-35 36.47088988404593 39.0 35.0 41.0 30.0 41.0 36-37 36.168667655521254 39.0 35.0 41.0 30.0 41.0 38-39 36.079047154466934 39.0 35.0 41.0 30.0 41.0 40-41 36.01262243102243 39.0 35.0 41.0 30.0 41.0 42-43 35.81398709289513 39.0 35.0 41.0 29.5 41.0 44-45 35.46858767741597 39.0 35.0 41.0 26.5 41.0 46-47 34.91360733870019 39.0 35.0 41.0 23.5 41.0 48-49 34.439255990913125 39.0 34.0 41.0 20.0 41.0 50-51 34.23423835744258 39.0 34.0 41.0 18.0 41.0 52-53 34.11362953120635 39.0 34.0 41.0 18.0 41.0 54-55 33.95029729449952 38.0 34.0 41.0 17.5 41.0 56-57 33.76681100067641 38.0 34.0 40.0 14.5 41.0 58-59 33.56071706610814 38.0 33.5 40.0 13.5 41.0 60-61 33.33600588774988 38.0 33.0 40.0 12.0 41.0 62-63 33.121132441757084 37.0 33.0 40.0 9.0 41.0 64-65 32.89079549853728 37.0 33.0 40.0 8.0 41.0 66-67 32.62270612442268 36.0 33.0 39.5 7.0 41.0 68-69 32.31311995439546 36.0 32.0 39.0 7.0 41.0 70-71 31.991497363133213 35.5 32.0 39.0 3.5 41.0 72-73 31.71278855575708 35.0 32.0 38.5 2.0 40.0 74-75 31.510728268715088 35.0 32.0 37.5 2.0 40.0 76-77 31.20376265788231 35.0 32.0 37.0 2.0 39.0 78-79 30.869929579561905 35.0 31.5 36.5 2.0 39.0 80-81 30.58110616526256 35.0 31.0 36.0 2.0 39.0 82-83 30.309414231281366 35.0 31.0 36.0 2.0 37.0 84-85 30.039556192418246 35.0 31.0 35.5 2.0 37.0 86-87 29.82709059789306 35.0 31.0 35.0 2.0 36.5 88-89 29.65269968646881 35.0 31.0 35.0 2.0 36.0 90-91 29.498938233930286 34.5 31.0 35.0 2.0 36.0 92-93 29.348608820564017 34.0 31.0 35.0 2.0 36.0 94-95 29.215492425477848 34.0 31.0 35.0 2.0 35.5 96-97 29.128327989642553 34.0 30.0 35.0 2.0 35.0 98-99 29.030108424218618 34.0 30.0 35.0 2.0 35.0 100-101 28.932388012926957 34.0 30.0 35.0 2.0 35.0 102-103 27.942591957095434 33.5 28.0 34.5 2.0 35.0 104-105 28.638224925658648 34.0 29.5 35.0 2.0 35.0 106-107 28.760273074179693 34.0 30.0 35.0 2.0 35.0 108-109 28.712244733002173 34.0 30.0 35.0 2.0 35.0 110-111 28.659736412584923 34.0 30.0 35.0 2.0 35.0 112-113 28.5591440924683 34.0 30.0 35.0 2.0 35.0 114-115 28.513951499802182 34.0 30.0 35.0 2.0 35.0 116-117 28.402846809313196 34.0 30.0 35.0 2.0 35.0 118-119 28.268323707126985 34.0 30.0 35.0 2.0 35.0 120-121 28.063441143062963 34.0 29.0 35.0 2.0 35.0 122-123 27.84008597646318 34.0 29.0 35.0 2.0 35.0 124-125 25.86633907313317 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19914217146483892 1101 2 0.6462193562815806 1101 3 0.4213378887768613 1101 4 0.15089390487990073 1101 5 0.42644104353546197 1101 6 0.30829101493251443 1101 7 0.5250392021453294 1101 8 0.4766060247402919 1101 9 0.39898959958890856 1101 10-11 0.42523656702767454 1101 12-13 0.4805091762123439 1101 14-15 0.5769709427423351 1101 16-17 0.6077428317170472 1101 18-19 0.5590227522268947 1101 20-21 0.4171872147318183 1101 22-23 0.4025915461940528 1101 24-25 0.404194947962786 1101 26-27 0.5152768496229925 1101 28-29 0.5537173736079382 1101 30-31 0.4199700049222912 1101 32-33 0.34210260826779404 1101 34-35 0.4942587846983528 1101 36-37 0.5997968213377973 1101 38-39 0.6084654008317756 1101 40-41 0.4608321229572212 1101 42-43 0.45754881800287706 1101 44-45 0.4138375617269574 1101 46-47 0.28515693660030195 1101 48-49 0.11100339298705819 1101 50-51 0.19993464490931245 1101 52-53 0.28301087252424395 1101 54-55 0.2651254474514957 1101 56-57 0.10752881490208921 1101 58-59 0.10702858952817706 1101 60-61 0.2469737919112589 1101 62-63 0.21652161873074505 1101 64-65 0.11865788390488774 1101 66-67 0.19136926800290155 1101 68-69 0.22215263990797496 1101 70-71 0.24823271033203298 1101 72-73 0.22331299399771254 1101 74-75 0.3433242506621852 1101 76-77 0.3485244361342197 1101 78-79 0.34364986929978514 1101 80-81 0.2572697479086443 1101 82-83 0.2914979317146802 1101 84-85 0.2623128568941304 1101 86-87 0.31466962694968004 1101 88-89 0.31983864516232785 1101 90-91 0.46646612202719595 1101 92-93 0.39883152299896807 1101 94-95 0.3156546105863498 1101 96-97 0.17957628941935155 1101 98-99 0.29206921165881283 1101 100-101 0.2791107166787725 1101 102-103 0.32518868691841263 1101 104-105 0.2942690404022663 1101 106-107 0.3611699043112466 1101 108-109 0.25989590775393623 1101 110-111 0.33792236007345977 1101 112-113 0.3899820739269231 1101 114-115 0.4154576493393236 1101 116-117 0.47366866499767113 1101 118-119 0.5562951802913929 1101 120-121 0.6433521522967425 1101 122-123 0.6476473789703263 1101 124-125 0.7025013103659106 1102 1 0.21472645192738682 1102 2 0.6369726666922411 1102 3 0.33483988873061676 1102 4 0.20475227974819177 1102 5 0.24889436418535382 1102 6 0.31166376931969353 1102 7 0.4063594523790144 1102 8 0.3412759580531528 1102 9 0.28244274848389495 1102 10-11 0.37822010663204253 1102 12-13 0.2799330953950516 1102 14-15 0.2620820632118921 1102 16-17 0.43889908904552755 1102 18-19 0.26388155813394576 1102 20-21 0.18847739134210428 1102 22-23 0.33585733360771997 1102 24-25 0.21034393873756585 1102 26-27 0.20281318239151602 1102 28-29 0.2760113668104083 1102 30-31 0.18576010226107798 1102 32-33 0.22728362802951096 1102 34-35 0.2610308989682082 1102 36-37 0.2928414908252037 1102 38-39 0.2635462494991856 1102 40-41 0.01769538293140016 1102 42-43 0.13583619038402617 1102 44-45 0.11108857491575463 1102 46-47 0.28457024496311334 1102 48-49 0.2583990353669279 1102 50-51 0.19709635380456092 1102 52-53 0.19302059153982754 1102 54-55 0.1533648192909567 1102 56-57 0.12019966148862693 1102 58-59 0.16103332765149503 1102 60-61 0.19610378725405297 1102 62-63 0.11425044911286619 1102 64-65 0.12558113423435913 1102 66-67 0.09844511596017469 1102 68-69 0.20317434213120578 1102 70-71 0.10391730226550067 1102 72-73 0.23710024747161995 1102 74-75 0.24696768417486936 1102 76-77 0.2666130352417575 1102 78-79 0.17217881330438445 1102 80-81 0.11083919469269787 1102 82-83 0.14137279009945303 1102 84-85 0.12626005712401778 1102 86-87 0.06861627393059422 1102 88-89 0.06769232814901116 1102 90-91 0.13473659141353878 1102 92-93 0.20911997215125666 1102 94-95 0.0910138508351288 1102 96-97 0.14199004038134433 1102 98-99 0.17102968986410616 1102 100-101 0.26443083314181237 1102 102-103 0.3584967524627274 1102 104-105 0.24188214265919328 1102 106-107 0.2748347824148105 1102 108-109 0.3714610408474712 1102 110-111 0.33659869125655817 1102 112-113 0.2724261627506337 1102 114-115 0.3779552133923474 1102 116-117 0.3057487242531103 1102 118-119 0.2788286394682231 1102 120-121 0.2938788122913927 1102 122-123 0.15052365433307813 1102 124-125 0.0018348104996803727 1103 1 0.12795379699777598 1103 2 0.5090969158804519 1103 3 0.2807851355191886 1103 4 0.15182466810574624 1103 5 0.3142640584076588 1103 6 0.1927837990154586 1103 7 0.2626265237490415 1103 8 0.28036760923958326 1103 9 0.264922164291967 1103 10-11 0.24621602017925426 1103 12-13 0.23461379199729038 1103 14-15 0.42916098857992324 1103 16-17 0.4750290063802751 1103 18-19 0.37167037327615304 1103 20-21 0.3120985420201947 1103 22-23 0.3823649603936943 1103 24-25 0.2529234052977998 1103 26-27 0.1956109736046585 1103 28-29 0.2678041747848994 1103 30-31 0.11354748845879215 1103 32-33 0.12087119851179295 1103 34-35 0.05174842620438369 1103 36-37 -0.017969747248450574 1103 38-39 0.12813528013279551 1103 40-41 -0.027000399711361922 1103 42-43 -0.23251203189403924 1103 44-45 -0.1575484748230167 1103 46-47 -0.07006934895385086 1103 48-49 -0.02192435776856172 1103 50-51 -0.1073961163148951 1103 52-53 -0.056834050312481565 1103 54-55 0.0022461364164456654 1103 56-57 -0.14712875430466 1103 58-59 -0.02998000752139518 1103 60-61 -0.14890582937820085 1103 62-63 -0.22876726815650983 1103 64-65 -0.21463166352400265 1103 66-67 -0.23467633939516475 1103 68-69 -0.3401146692855619 1103 70-71 -0.3190299450453793 1103 72-73 -0.24250772899513962 1103 74-75 -0.18467620971532384 1103 76-77 -0.15433040483537397 1103 78-79 -0.12755276686186434 1103 80-81 -0.07781001749899019 1103 82-83 -0.12854024959399624 1103 84-85 -0.13741366885698625 1103 86-87 -0.14018085044178008 1103 88-89 -0.07894961120789645 1103 90-91 -0.04571261453678588 1103 92-93 -0.14151406037940362 1103 94-95 -0.11054862606770754 1103 96-97 -0.17690602607935446 1103 98-99 -0.13008320332147605 1103 100-101 -0.21246112222912927 1103 102-103 -0.10319008367347493 1103 104-105 -0.250067315409499 1103 106-107 -0.231992739056313 1103 108-109 -0.08953062200046347 1103 110-111 -0.018022014313533674 1103 112-113 -0.13349467700884432 1103 114-115 -0.14018047392174893 1103 116-117 -0.04153755410199267 1103 118-119 -0.0028370581441237164 1103 120-121 -0.24204887654690665 1103 122-123 -0.2763915829784729 1103 124-125 -0.060355482203995336 1104 1 0.05967054448758091 1104 2 0.2930949180603406 1104 3 0.14622936755172233 1104 4 0.08131662638771786 1104 5 0.37887971997233905 1104 6 0.17200884820235274 1104 7 0.29606745724607464 1104 8 0.18365376088528507 1104 9 0.25463843447007406 1104 10-11 0.23672247905950883 1104 12-13 0.19831677946006465 1104 14-15 0.03208296451914805 1104 16-17 0.2017408905395044 1104 18-19 0.14170864842584763 1104 20-21 0.05255548596231563 1104 22-23 0.05697205987217302 1104 24-25 0.06138332464689 1104 26-27 0.04671945212029982 1104 28-29 0.036307076790834 1104 30-31 -0.10651739968871254 1104 32-33 -0.2615104228553662 1104 34-35 -0.1652358741772204 1104 36-37 -0.14158353401149526 1104 38-39 -0.07010592466098586 1104 40-41 -0.1311635295967335 1104 42-43 -0.09328504721639774 1104 44-45 -0.2152431167121165 1104 46-47 -0.3469662119006074 1104 48-49 -0.30065973189646655 1104 50-51 -0.367667749319601 1104 52-53 -0.4886227636129945 1104 54-55 -0.381916792538739 1104 56-57 -0.3074095316830352 1104 58-59 -0.25848703884764035 1104 60-61 -0.20762665041993955 1104 62-63 -0.4036612057238145 1104 64-65 -0.4505655255087504 1104 66-67 -0.4086441528401039 1104 68-69 -0.43668509859545424 1104 70-71 -0.3687838465764699 1104 72-73 -0.44678176004008563 1104 74-75 -0.4983421686156717 1104 76-77 -0.4627640802420956 1104 78-79 -0.4217769877243249 1104 80-81 -0.449108801502323 1104 82-83 -0.4929813684388691 1104 84-85 -0.319053632312297 1104 86-87 -0.2563385896573678 1104 88-89 -0.27705252272726355 1104 90-91 -0.29837504198663467 1104 92-93 -0.31123373816939903 1104 94-95 -0.3380436253664989 1104 96-97 -0.25123194631909485 1104 98-99 -0.2621754237148224 1104 100-101 -0.1975587660971918 1104 102-103 -0.11872290951457032 1104 104-105 -0.24041569554315956 1104 106-107 -0.25674436474552564 1104 108-109 -0.24956320959577383 1104 110-111 -0.22397514509284022 1104 112-113 -0.158927312499344 1104 114-115 -0.29412169897257456 1104 116-117 -0.21478007109289265 1104 118-119 -0.20051904143165444 1104 120-121 -0.20411161761187557 1104 122-123 -0.1074371826484537 1104 124-125 -0.13215050225190694 1105 1 0.19268932198873046 1105 2 0.5526636871445163 1105 3 0.3024499937261709 1105 4 0.19585088588339516 1105 5 0.39298419031116794 1105 6 0.2763142707464681 1105 7 0.2942167300437859 1105 8 0.18140413360816865 1105 9 0.34348018652135437 1105 10-11 0.15165598094708344 1105 12-13 0.22039864720646563 1105 14-15 0.2741715134894207 1105 16-17 0.411229074156779 1105 18-19 0.3302607196857963 1105 20-21 0.26906982824233694 1105 22-23 0.26992479434125727 1105 24-25 0.2514668620951639 1105 26-27 0.2304942872241469 1105 28-29 0.2517272126061414 1105 30-31 0.16411878250129774 1105 32-33 0.13507901170930836 1105 34-35 0.10587391375533883 1105 36-37 0.13423331507661374 1105 38-39 0.13709326521545506 1105 40-41 0.05070349189836776 1105 42-43 -0.07917705302531886 1105 44-45 -0.06408858189686129 1105 46-47 8.695185919549431E-4 1105 48-49 -0.27850487006771374 1105 50-51 -0.1732285291259572 1105 52-53 -0.256254610055187 1105 54-55 -0.24272764906439193 1105 56-57 -0.1862941257231796 1105 58-59 -0.10418509988318192 1105 60-61 -0.25899988557404896 1105 62-63 -0.09401910024021021 1105 64-65 -0.1392803987426987 1105 66-67 -0.0954151684908382 1105 68-69 -0.12878760202063688 1105 70-71 -0.19872827441965057 1105 72-73 -0.18839690463078895 1105 74-75 -0.19489237767071899 1105 76-77 -0.06441533727066684 1105 78-79 -0.19032342484792508 1105 80-81 -0.2144591046341624 1105 82-83 -0.13027457872062342 1105 84-85 -0.06839567875626784 1105 86-87 -0.04625336478726183 1105 88-89 -0.2214713045778538 1105 90-91 -0.26939830424420563 1105 92-93 -0.143846728981611 1105 94-95 -0.1829664389067922 1105 96-97 -0.276872300750167 1105 98-99 -0.2168913045691454 1105 100-101 -0.17629966751067982 1105 102-103 -0.15779538327495857 1105 104-105 -0.20807357821941963 1105 106-107 -0.23827958031265695 1105 108-109 -0.3041960080821653 1105 110-111 -0.40902158745187833 1105 112-113 -0.3061589943071681 1105 114-115 -0.2826426999238123 1105 116-117 -0.27858344335908924 1105 118-119 -0.2997881743384738 1105 120-121 -0.4074720235274363 1105 122-123 -0.39870984907294726 1105 124-125 -0.27119214733917474 1106 1 0.13901248839712466 1106 2 0.40828905950152716 1106 3 0.19654308557144518 1106 4 0.10893348752937726 1106 5 0.2271135468383676 1106 6 0.00800208184153206 1106 7 0.16635900212540378 1106 8 0.13694061881318476 1106 9 0.033990595769125775 1106 10-11 0.04590389754230273 1106 12-13 0.11018336403967055 1106 14-15 -0.03784362484195469 1106 16-17 0.09401738715121155 1106 18-19 0.05766208844424625 1106 20-21 0.004859647812182288 1106 22-23 -0.013244289837949452 1106 24-25 -0.004924020451767319 1106 26-27 0.09676408545151105 1106 28-29 -0.10020808679880133 1106 30-31 -0.06415939480564248 1106 32-33 -0.07852550785742807 1106 34-35 -0.05156570957687734 1106 36-37 0.09319079918339668 1106 38-39 0.010680003364029744 1106 40-41 -0.03311322430956665 1106 42-43 0.017028534303811682 1106 44-45 0.06790724371683154 1106 46-47 0.04767594159589805 1106 48-49 0.05840824144518564 1106 50-51 -0.062232941182706725 1106 52-53 0.004768652936725459 1106 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62-63 -0.7861392327744596 2216 64-65 -0.7923965875280956 2216 66-67 -0.8103163230920245 2216 68-69 -0.6706062407082065 2216 70-71 -0.6323867446509794 2216 72-73 -0.6792970597121659 2216 74-75 -0.6299114053001347 2216 76-77 -0.5717639251992495 2216 78-79 -0.6414571128203725 2216 80-81 -0.6024307904989072 2216 82-83 -0.6155741070846972 2216 84-85 -0.5107617962330018 2216 86-87 -0.47496267390414815 2216 88-89 -0.6887829717944847 2216 90-91 -0.5557888951753398 2216 92-93 -0.5030011240324299 2216 94-95 -0.572030625496609 2216 96-97 -0.6976357868771395 2216 98-99 -0.6742315431915209 2216 100-101 -0.8776202161519819 2216 102-103 -0.7517462510035742 2216 104-105 -0.6383253117990542 2216 106-107 -0.6534640120924315 2216 108-109 -0.7788578611807182 2216 110-111 -0.6898601893705454 2216 112-113 -0.698432470438938 2216 114-115 -0.8349879561196794 2216 116-117 -0.7238007090851504 2216 118-119 -0.6264497861460718 2216 120-121 -0.5869864015121102 2216 122-123 -0.6569873472532777 2216 124-125 -0.6893530668334833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95.0 3 75.0 4 232.0 5 488.0 6 1177.0 7 2388.0 8 3841.0 9 6676.0 10 11719.0 11 14471.0 12 15283.0 13 19320.0 14 27508.0 15 20262.0 16 9128.0 17 7454.0 18 7012.0 19 6778.0 20 6322.0 21 6524.0 22 7026.0 23 8029.0 24 9535.0 25 11796.0 26 14403.0 27 17955.0 28 22221.0 29 27592.0 30 34682.0 31 42776.0 32 54262.0 33 68357.0 34 93442.0 35 129640.0 36 206563.0 37 344363.0 38 150129.0 39 862.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.92632009126977 17.56971523603567 11.710896748230514 19.793067924464044 2 18.678414784079774 22.652657223362183 37.66766734118841 21.00126065136963 3 17.59908280428195 30.158197826694256 32.88291290469418 19.359806464329623 4 10.7350196825829 16.851368138039003 36.98687733269804 35.426734846680056 5 9.302998783912454 43.65974239377681 34.640118969404824 12.39713985290591 6 27.972484128458454 37.64451717473089 19.2067987061698 15.17619999064086 7 25.184098537563475 36.26319319675606 22.3570001403871 16.195708125293358 8 28.68236071543535 32.672474060292714 20.47453675802227 18.170628466249664 9 28.425338878860114 10.781729257096993 20.31784206593089 40.47508979811201 10-11 24.297461829598422 26.47048396680058 29.371214688744786 19.860839514856217 12-13 26.182016837943657 26.411244864880963 29.748380939686015 17.65835735748937 14-15 22.872390962474103 26.465378981356874 29.929962435815444 20.73226762035358 16-17 19.784136955512146 28.41550356817465 30.433285946745038 21.367073529568167 18-19 18.948748782248266 25.35830616582978 35.86277090101575 19.830174150906206 20-21 20.58776817126659 24.27434758995055 36.574278247231604 18.56360599155125 22-23 20.061174742233685 24.870425543078277 36.170807140740195 18.89759257394784 24-25 19.449675478911445 24.92952283984668 36.528687891116334 19.092113790125538 26-27 19.32747488985285 24.91700853525205 36.467321711928506 19.28819486296659 28-29 19.221121026442407 24.93094089135882 36.35189231884037 19.4960457633584 30-31 19.611191546144106 24.671366562061735 36.63092940514157 19.08651248665259 32-33 19.43365149682427 24.9399100671731 36.220403492377265 19.40603494362536 34-35 19.80334454657964 25.070131534959767 35.968357587340485 19.158166331120107 36-37 19.934897255077686 25.04197432475932 35.3998834361657 19.62324498399729 38-39 19.973184645905448 25.353981108717754 35.2684655122782 19.404368733098597 40-41 20.01860484243492 25.362569311723636 34.9787345374722 19.640091308369236 42-43 19.822554118831164 25.43887295701656 34.45333076185942 20.285242162292857 44-45 19.89113917408443 25.788074520596126 33.910835098398074 20.40995120692137 46-47 20.235528956269544 26.00082965190075 32.79021861682137 20.97342277500833 48-49 20.553580539129257 26.462226171073254 32.023133396749735 20.961059893047747 50-51 20.707310112052813 26.44744189509474 31.94286919726507 20.902378795587378 52-53 20.751708080975323 26.634540692762403 31.75539776987786 20.858353456384418 54-55 20.205420200243925 26.82772215446578 31.84856907848087 21.11828856680943 56-57 20.661720180331194 26.97547461734929 31.341230977370678 21.021574224948843 58-59 21.061403375046044 26.447373878934698 31.427692196424715 21.063530549594542 60-61 21.325233204854406 26.538201918959007 31.647153752123593 20.489411124062997 62-63 20.829392253598023 26.489869947913935 31.758071354932575 20.922666443555464 64-65 20.50418017100732 26.32710059708625 32.164095553653745 21.00462367825269 66-67 20.902434553925097 26.86369000961115 31.278155877959968 20.95571955850378 68-69 21.035116491286285 26.608646142872438 31.35582825130835 21.000409114532925 70-71 20.642967805521383 26.501398603877803 31.670418730301662 21.185214860299148 72-73 20.929370981911465 26.76342104498132 31.13612239876739 21.171085574339823 74-75 20.427200106071496 26.87373769781607 31.152084613287514 21.546977582824926 76-77 20.227464810702426 26.99521828283602 31.277811811351896 21.499505095109658 78-79 20.61652025849707 26.82770324525209 31.071371722655577 21.484404773595266 80-81 20.42420542577947 26.997230529687787 30.755974639422906 21.822589405109834 82-83 20.746031397145316 26.93704476018216 30.607962517982706 21.708961324689817 84-85 20.455778198626042 26.939992725359634 30.634283408927672 21.969945667086648 86-87 20.35744242158679 26.941372579466115 30.50349515485532 22.197689844091773 88-89 20.496084522830802 26.702565187935463 30.694582854290502 22.106767434943237 90-91 20.55956184034712 26.7296461489974 30.724708379849446 21.986083630806036 92-93 20.530185422382495 26.695923671336942 30.54096624690818 22.232924659372387 94-95 20.264044655027856 26.651245154188537 30.550299833199503 22.534410357584107 96-97 20.501389538348526 26.78868275167945 30.421122826397934 22.28880488357409 98-99 20.15185549159998 26.714463177329943 30.139596158127674 22.9940851729424 100-101 20.147089061735574 26.75067535469416 30.330835170912597 22.771400412657673 102-103 20.38400752146788 26.633923323286275 30.013230636368082 22.968838518877764 104-105 20.087657786497314 26.407484271868892 30.34662510786169 23.158232833772107 106-107 19.96091114677535 26.638064375009087 30.330659933712734 23.070364544502834 108-109 20.37888010709269 26.711841669579623 29.666285442016154 23.242992781311532 110-111 20.12884571342677 26.868606726324813 29.698646240806486 23.303901319441934 112-113 20.109940838724363 26.716247829228635 29.556598896762402 23.6172124352846 114-115 19.671871310068102 26.772816145386773 29.597358177190486 23.95795436735464 116-117 19.577516727359114 26.69927630015992 29.727646326718034 23.995560645762932 118-119 19.796392965193643 26.47643110118198 29.620487350701115 24.106688582923262 120-121 19.591961908408585 26.58303108946064 29.405439326093664 24.41956767603711 122-123 19.63229379057473 26.495417410754644 29.25493349386843 24.617355304802192 124-125 19.319476677607625 26.35551234952638 29.150455542343206 25.174555430522787 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 487.0 1 403.0 2 1238.0 3 2208.0 4 1631.0 5 1361.5 6 2085.5 7 4977.0 8 7693.0 9 8435.5 10 6895.0 11 4312.0 12 3764.5 13 3813.0 14 3982.0 15 4297.0 16 4582.0 17 4734.0 18 5059.0 19 5309.0 20 5696.0 21 6338.0 22 7122.5 23 8200.0 24 9552.5 25 10975.5 26 13099.5 27 15720.5 28 17364.0 29 19459.0 30 22858.0 31 25501.5 32 27769.5 33 30955.0 34 35106.5 35 37747.0 36 40225.0 37 44075.0 38 44496.5 39 43081.5 40 42299.0 41 42164.5 42 43127.0 43 44322.0 44 45978.0 45 47196.5 46 46664.0 47 45417.0 48 45200.0 49 44937.0 50 44513.5 51 42505.5 52 39971.0 53 38692.5 54 36319.0 55 32939.5 56 29500.0 57 25790.5 58 22932.0 59 20902.5 60 18416.5 61 15824.0 62 13049.5 63 9582.5 64 6677.5 65 5078.5 66 3870.0 67 3195.5 68 2543.5 69 1695.5 70 1237.5 71 1080.5 72 961.5 73 970.5 74 803.0 75 605.5 76 425.5 77 262.5 78 170.0 79 94.0 80 54.5 81 26.5 82 13.5 83 4.5 84 1.0 85 1.0 86 1.5 87 0.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06650661591932988 2 2.8361030242784595E-4 3 0.0 4 1.4180515121392298E-4 5 0.06721564167539949 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.090257560696149E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.5451287803480744E-5 36-37 0.0 38-39 0.0 40-41 3.5451287803480744E-5 42-43 0.0028715543120819407 44-45 0.010280873463009418 46-47 0.011060801794685994 48-49 0.009252786116708475 50-51 0.008047442331390129 52-53 0.007409319150927477 54-55 0.0075156730143379185 56-57 0.006523036955840457 58-59 0.0045732161266490165 60-61 0.002694297873064537 62-63 0.001985272116994922 64-65 0.0026233952974575757 66-67 0.0032260671901167483 68-69 0.0016307592389601144 70-71 0.0036160313559550367 72-73 0.003190615902313268 74-75 0.0011698924975148647 76-77 9.92636058497461E-4 78-79 1.7725643901740374E-4 80-81 5.672206048556919E-4 82-83 2.1270772682088448E-4 84-85 4.963180292487305E-4 86-87 3.545128780348075E-4 88-89 0.0015244053755496723 90-91 0.0023043337072262485 92-93 8.153796194800572E-4 94-95 0.0013116976487287878 96-97 0.0018080156779775184 98-99 0.0022688824194227676 100-101 0.0011344412097113838 102-103 0.0016307592389601144 104-105 0.0010635386341044226 106-107 6.735744682661342E-4 108-109 0.0013471489365322685 110-111 0.0012053437853183455 112-113 0.012266145580004339 114-115 0.021199870106481486 116-117 0.01921459798948657 118-119 0.012017986565379975 120-121 0.007161160136303111 122-123 0.007267513999713554 124-125 0.006310329229019573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1410386.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.628388576688227 #Duplication Level Percentage of deduplicated Percentage of total 1 44.911343165738174 11.510053541525291 2 14.79264528644381 7.5822332295619495 3 10.944707474797653 8.414856480668954 4 8.069615570220826 8.272449739924514 5 5.844713149919187 7.489528986270417 6 3.9131001801834215 6.0171871174349665 7 2.792812574409178 5.01027001151736 8 1.8830379009969704 3.860738162510539 9 1.2886946653789835 2.972445087693402 >10 5.11080547711366 21.76188303437682 >50 0.28636955936284375 5.0658801586276425 >100 0.1537531856996542 7.110889532706051 >500 0.005321146166022382 0.9065893709546968 >1k 0.0022404825962199504 1.237328332348034 >5k 0.0 0.0 >10k+ 8.401809735824814E-4 2.7876672138793026 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14973 1.0616242645630345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12329 0.8741578546582283 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11537 0.8180030147775148 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 0.2679408332187075 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3020 0.2141257783330237 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.1852684300609904 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2169 0.1537876864914995 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 0.13095705714605788 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1552 0.11004079734200424 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.09025756069615E-5 0.0 22-23 0.0 0.0 0.0 2.1270772682088448E-4 0.0 24-25 0.0 0.0 0.0 4.25415453641769E-4 0.0 26-27 0.0 0.0 0.0 6.026718926591728E-4 0.0 28-29 0.0 0.0 0.0 0.0010280873463009417 0.0 30-31 0.0 0.0 0.0 0.004041446809596805 0.0 32-33 0.0 0.0 0.0 0.011025350506882513 0.0 34-35 0.0 0.0 0.0 0.020951711091857124 0.0 36-37 0.0 0.0 0.0 0.03626666742296081 0.0 38-39 0.0 0.0 0.0 0.06267787683655396 0.0 40-41 0.0 0.0 0.0 0.09493854873772145 0.0 42-43 0.0 0.0 0.0 0.13574298099952778 0.0 44-45 0.0 0.0 0.0 0.1808724703733588 0.0 46-47 0.0 0.0 0.0 0.23571561260534352 0.0 48-49 0.0 0.0 0.0 0.2925794782421266 0.0 50-51 0.0 0.0 0.0 0.35160587243492203 0.0 52-53 0.0 0.0 0.0 0.4197432475932121 0.0 54-55 0.0 0.0 0.0 0.5032310303704093 0.0 56-57 0.0 0.0 0.0 0.611180201732008 0.0 58-59 0.0 0.0 0.0 0.7345861345759246 0.0 60-61 0.0 0.0 0.0 0.8511499688737694 0.0 62-63 0.0 0.0 0.0 0.9711171268007481 0.0 64-65 0.0 0.0 0.0 1.100159814405418 0.0 66-67 0.0 0.0 0.0 1.2701487394231084 0.0 68-69 0.0 0.0 0.0 1.4823601482147442 0.0 70-71 0.0 0.0 0.0 1.7061286768303146 0.0 72-73 0.0 0.0 0.0 1.9157875929000996 0.0 74-75 0.0 0.0 0.0 2.149872446266483 0.0 76-77 0.0 0.0 0.0 2.4176005717583697 0.0 78-79 0.0 0.0 0.0 2.7535369749841534 0.0 80-81 0.0 0.0 0.0 3.092593091536643 0.0 82-83 0.0 0.0 0.0 3.427005089386877 0.0 84-85 0.0 0.0 0.0 3.7821206393143436 0.0 86-87 0.0 0.0 0.0 4.184599109747261 0.0 88-89 0.0 0.0 0.0 4.653016975494651 0.0 90-91 0.0 0.0 0.0 5.146995219748352 0.0 92-93 0.0 0.0 0.0 5.609421817856956 0.0 94-95 0.0 0.0 0.0 6.128747732890146 0.0 96-97 0.0 0.0 0.0 6.678029986117275 0.0 98-99 0.0 0.0 0.0 7.316153166579929 0.0 100-101 0.0 0.0 0.0 7.999441287704217 0.0 102-103 0.0 0.0 0.0 8.657665348351443 0.0 104-105 0.0 0.0 0.0 9.33014082669567 0.0 106-107 0.0 0.0 0.0 10.053524354325695 0.0 108-109 0.0 0.0 0.0 10.810905666959258 0.0 110-111 0.0 0.0 0.0 11.624087306595499 0.0 112-113 0.0 0.0 0.0 12.417061712183756 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6965 0.0 86.22575 1 ATAACGC 230 0.0 56.944706 3 GTATCAA 11555 0.0 51.933678 2 TAACGCA 250 0.0 50.007805 4 GTACATG 15385 0.0 49.026825 1 ACATGGG 15790 0.0 47.20421 3 TACATGG 15985 0.0 47.149765 2 CATGGGA 11705 0.0 45.419098 4 TATAACG 265 0.0 44.930637 2 TACCTGG 1290 0.0 44.303703 2 GTCGGGA 620 0.0 44.169724 2 ATGGGAT 4750 0.0 41.35984 5 GGAACGC 320 0.0 40.901432 8 ATGGGAG 3610 0.0 40.07353 5 CATGGGG 3760 0.0 39.108177 4 ATCCACG 235 0.0 37.99985 2 ATCAACG 16425 0.0 37.91271 4 TCAACGC 16500 0.0 37.77646 5 CAACGCA 16695 0.0 37.41698 6 GGGAACG 415 0.0 37.27272 7 >>END_MODULE