FastQCFastQC Report
Thu 26 May 2016
SRR936256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342473
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58270.13418621140534437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55850.061.465211
GTACATG315750.055.415131
TACATGG322350.053.4321482
ACATGGG320600.051.9979633
CATGGGA207950.048.7806054
GTATCAA78800.043.715362
ATGGGAG80500.041.6096655
CATGGGG110150.041.0497744
ATGGGAT88900.040.1542245
TCAACGC94300.036.9715465
CAACGCA95350.036.627236
ATCAACG96050.036.2359964
AACGCAG100200.034.854367
TATCAAC112050.031.3803183
GAGTACA187200.030.1921791
ATGGGGG59100.029.0932395
AGTACAT187750.028.7731652
ACGCAGA120000.028.5580128
TACCTGG36300.028.3544482
CGATACC11400.028.181274118-119
Produced by FastQC (version 0.11.5)