FastQCFastQC Report
Thu 26 May 2016
SRR936256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342473
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184260.4243204275536083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157130.3618445065749401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150900.34749784281905727No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48950.11272378665336548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102450.081.97621
GTATCAA164550.050.973972
GTACATG278950.046.5917471
ACATGGG280700.045.107673
TACATGG285950.045.0508042
CATGGGA179450.040.8688664
ATCAACG234050.037.159254
TCAACGC234250.037.1021085
CAACGCA236200.036.849766
CATGGGG100100.036.6924254
AACGCAG243350.035.7915047
TATCAAC244800.035.673363
ATGGGAG72600.034.192025
ATGGGAT76800.031.7795665
ACGCAGA285000.031.1871726
CGCAGAG288250.030.81497
GAGTACA185000.029.0734521
AGTACAT183600.027.7871192
GCAGAGT323100.027.4543368
CAGAGTA322500.027.3024869
Produced by FastQC (version 0.11.5)