FastQCFastQC Report
Thu 26 May 2016
SRR936255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466955
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.065.205691
GTACATG218850.055.917121
TACATGG220300.054.6860282
ACATGGG219550.052.8415833
CATGGGA151000.049.4471664
GTATCAA52550.043.813112
CATGGGG65550.042.113364
ATGGGAG55600.041.5175365
ATGGGAT61050.041.0271225
TCAACGC62850.036.9176455
CAACGCA64450.035.6319056
ATCAACG65400.035.3872224
AACGCAG66850.034.3526737
GTCGGGA5600.032.9336242
GAGTACA125500.032.5190161
AGTACAT122300.031.9112072
ATGGGAC31400.031.8312915
TATCAAC77700.030.1682323
TACCTGG24700.030.1077922
ATGGGGA34400.030.0929995
Produced by FastQC (version 0.11.5)