FastQCFastQC Report
Thu 26 May 2016
SRR936255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466955
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103730.42047787657253577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81550.3305694672176833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76110.3085179908024265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67300.081.082941
GTATCAA102800.052.6405372
GTACATG184100.046.694061
ACATGGG187450.045.4468423
TACATGG188600.045.264422
CATGGGA130050.041.7936174
TCAACGC142800.039.3127145
ATCAACG143650.039.0800934
CAACGCA145100.038.9112666
AACGCAG149550.037.992127
CATGGGG54650.036.928284
TATCAAC153550.036.87063
ATGGGAG44050.035.6785775
ATGGGAT54850.035.5997355
GAGTACA120300.032.188211
ACGCAGA174850.031.9163938
CGCAGAG176700.031.6159049
AGTACAT118800.031.2191542
TACCTGG19700.030.823612
GCAGAGT196050.026.522918
Produced by FastQC (version 0.11.5)