FastQCFastQC Report
Thu 26 May 2016
SRR936254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036507
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15890.15330335443947798No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10420.1005299530056237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.058.7734871
GTACATG116100.055.138051
TACATGG116200.052.9914252
ACATGGG115450.050.7590753
CATGGGA80900.048.0950744
TAGATCG505.33512E-447.5955
CATGGGG31750.044.784274
ATGGGAT29450.044.039525
GTATCAA28500.042.3762552
ATGGGAG29150.040.8190425
ATGGGGG15050.040.3213465
TACCTGG8550.036.1833342
GTCGGGA4200.035.412952
CCGTTGC1355.8627847E-835.2555589
GAGTACA65250.034.738881
CAACGCA36950.033.812426
TCAACGC36800.033.6269075
ATCAACG37100.033.354994
AACGCAG37950.032.9214447
AGTACAT66550.032.719332
Produced by FastQC (version 0.11.5)