##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936254_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036507 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.108762410673542 31.0 30.0 33.0 23.0 34.0 2 29.234494316005584 31.0 30.0 34.0 23.0 34.0 3 29.45109102012818 31.0 30.0 34.0 23.0 34.0 4 32.726178404969765 35.0 35.0 37.0 22.0 37.0 5 33.17142768934508 37.0 35.0 37.0 27.0 37.0 6 32.957576745743154 37.0 35.0 37.0 25.0 37.0 7 32.921024170603765 37.0 35.0 37.0 25.0 37.0 8 32.66896798574443 37.0 33.0 37.0 23.0 37.0 9 33.92328368259935 39.0 35.0 39.0 17.0 39.0 10-11 33.90482022793864 38.5 34.5 39.0 18.5 39.0 12-13 33.891870484232136 38.5 34.5 39.0 18.5 39.0 14-15 34.98566435151909 39.0 35.0 41.0 20.0 41.0 16-17 35.04601078429765 39.0 35.0 41.0 22.0 41.0 18-19 35.125135672021514 39.0 35.0 41.0 22.5 41.0 20-21 35.27875065001973 39.0 35.0 41.0 24.5 41.0 22-23 35.208357493002936 39.0 34.0 41.0 24.5 41.0 24-25 35.22073849959527 39.0 34.0 41.0 25.5 41.0 26-27 35.323101532358194 39.0 34.0 41.0 26.5 41.0 28-29 35.22058654693119 39.0 34.0 41.0 26.0 41.0 30-31 35.12612215836458 39.0 34.0 41.0 26.0 41.0 32-33 34.94489038665441 39.0 34.0 41.0 25.0 41.0 34-35 34.89021540616706 39.0 34.0 41.0 25.0 41.0 36-37 34.773030958787544 39.0 33.5 41.0 25.0 41.0 38-39 34.518417627666764 38.0 33.0 40.0 24.0 41.0 40-41 34.603831908515815 38.5 33.0 40.5 24.0 41.0 42-43 34.519692582876914 39.0 33.0 41.0 24.0 41.0 44-45 34.42687796609188 38.5 33.0 41.0 23.5 41.0 46-47 34.405360021688224 38.0 33.0 40.0 24.0 41.0 48-49 34.29915427488671 38.0 33.0 40.0 23.5 41.0 50-51 34.16288505528665 38.0 33.0 40.0 23.0 41.0 52-53 34.008468828478726 38.0 33.0 40.0 23.0 41.0 54-55 33.83688098584959 38.0 32.5 40.0 22.5 41.0 56-57 33.63423739540592 38.0 32.0 40.0 22.0 41.0 58-59 33.386988703404796 37.5 32.0 40.0 20.5 41.0 60-61 33.12798128715002 37.0 31.5 40.0 19.5 41.0 62-63 32.867953134904056 36.5 31.0 40.0 18.0 41.0 64-65 32.5724433120085 36.0 31.0 39.5 17.0 41.0 66-67 32.26174304659785 36.0 31.0 39.0 13.5 41.0 68-69 31.9478435746213 35.0 31.0 39.0 11.5 41.0 70-71 31.60176631706298 35.0 31.0 38.5 8.0 40.0 72-73 31.275276481490238 35.0 30.5 37.5 7.0 40.0 74-75 30.92337051269311 35.0 30.0 37.0 4.0 39.5 76-77 30.59435971006467 35.0 30.0 36.5 2.0 39.0 78-79 30.247238079434098 34.5 30.0 36.0 2.0 39.0 80-81 29.934452444604812 34.0 30.0 36.0 2.0 37.5 82-83 29.62712649311582 34.0 29.0 35.0 2.0 37.0 84-85 29.64784270632036 34.0 30.0 35.0 2.0 37.0 86-87 29.541606086596616 34.0 30.0 35.0 2.0 36.0 88-89 29.359261924907408 34.0 30.0 35.0 2.0 36.0 90-91 29.18339335865556 34.0 29.5 35.0 2.0 36.0 92-93 29.05642653643439 34.0 29.0 35.0 2.0 35.5 94-95 28.918053616618124 34.0 29.0 35.0 2.0 35.0 96-97 28.814421417317973 34.0 29.0 35.0 2.0 35.0 98-99 28.69722394542439 34.0 29.0 35.0 2.0 35.0 100-101 28.57428796911164 34.0 29.0 35.0 2.0 35.0 102-103 27.613152154302867 32.5 26.5 34.5 2.0 35.0 104-105 28.242903810586903 34.0 29.0 35.0 2.0 35.0 106-107 28.346054585256056 34.0 29.0 35.0 2.0 35.0 108-109 28.284974920574584 34.0 29.0 35.0 2.0 35.0 110-111 28.15010270070535 34.0 29.0 35.0 2.0 35.0 112-113 28.086202987534094 34.0 29.0 35.0 2.0 35.0 114-115 27.952288310643343 34.0 28.0 35.0 2.0 35.0 116-117 27.813573859124926 34.0 28.0 35.0 2.0 35.0 118-119 27.596885018625052 34.0 27.0 35.0 2.0 35.0 120-121 27.369298036578623 34.0 27.0 35.0 2.0 35.0 122-123 27.058516247357712 34.0 27.0 35.0 2.0 35.0 124-125 25.103764373998438 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5267839412980813 1101 2 0.5733211454813727 1101 3 0.3290857530989335 1101 4 0.470775469951775 1101 5 0.4617453771950295 1101 6 0.510693833069098 1101 7 0.5445094363618921 1101 8 0.3876340241869656 1101 9 0.5775687158267573 1101 10-11 0.4463093966152556 1101 12-13 0.46260559717877214 1101 14-15 0.4711763136381819 1101 16-17 0.5787417287809902 1101 18-19 0.46788449375940644 1101 20-21 0.40424289460734997 1101 22-23 0.540994885657156 1101 24-25 0.4555453923193298 1101 26-27 0.35291943000004977 1101 28-29 0.41383416789656735 1101 30-31 0.4476058483792045 1101 32-33 0.6415656578344624 1101 34-35 0.5914318197743285 1101 36-37 0.5040048044790879 1101 38-39 0.539679986002028 1101 40-41 0.5674773324871225 1101 42-43 0.6622348810288301 1101 44-45 0.49785725570946937 1101 46-47 0.46343594414958034 1101 48-49 0.38284461691709737 1101 50-51 0.370308253490343 1101 52-53 0.27402452675330125 1101 54-55 0.08151869812283508 1101 56-57 0.22721331054768257 1101 58-59 0.18347640857324166 1101 60-61 0.1884875377728008 1101 62-63 0.15802516208231054 1101 64-65 0.13795246684468054 1101 66-67 0.3330079383422415 1101 68-69 0.26117182035434894 1101 70-71 0.29979331422109823 1101 72-73 0.23797771344283092 1101 74-75 0.3733716446408444 1101 76-77 0.27231268706839273 1101 78-79 0.28954482465305276 1101 80-81 0.26506671892608225 1101 82-83 0.42175908926110495 1101 84-85 0.23730130042778796 1101 86-87 0.2748409810129502 1101 88-89 0.2786033928031344 1101 90-91 0.2888789636652618 1101 92-93 0.29572190086382477 1101 94-95 0.4861311639283983 1101 96-97 0.5428785852106657 1101 98-99 0.5271184118834888 1101 100-101 0.33218060695579155 1101 102-103 0.37769363857308846 1101 104-105 0.46207810652908066 1101 106-107 0.3915304255803882 1101 108-109 0.38668977167300866 1101 110-111 0.47903608311009904 1101 112-113 0.32286204558261034 1101 114-115 0.3611232943396878 1101 116-117 0.40400973395787076 1101 118-119 0.4423867110023778 1101 120-121 0.4078828480669472 1101 122-123 0.3625602048606851 1101 124-125 0.3528108497312452 1102 1 0.44397731634184723 1102 2 0.5192574229014149 1102 3 0.5081308277666956 1102 4 0.7208142880547115 1102 5 0.5349213069266483 1102 6 0.6827166368017288 1102 7 0.5741238432060527 1102 8 0.5965492484947106 1102 9 0.47336494362051695 1102 10-11 0.7738508406486773 1102 12-13 0.5678780089246729 1102 14-15 0.6888259916762678 1102 16-17 0.6475726949711813 1102 18-19 0.5901181330707317 1102 20-21 0.614206968833642 1102 22-23 0.6886125197318478 1102 24-25 0.6522527038231942 1102 26-27 0.6103549995605633 1102 28-29 0.6053379507587664 1102 30-31 0.5815617936380661 1102 32-33 0.646312502912167 1102 34-35 0.6299176854180217 1102 36-37 0.6093304959741204 1102 38-39 0.6221501873994768 1102 40-41 0.6189954247676397 1102 42-43 0.7072414268265774 1102 44-45 0.7133540513131322 1102 46-47 0.7680777520075779 1102 48-49 0.7040663215646106 1102 50-51 0.7429053368046468 1102 52-53 0.7961291529577963 1102 54-55 0.7378528991549445 1102 56-57 0.7970113041845579 1102 58-59 0.7826227908782784 1102 60-61 0.6267157577724944 1102 62-63 0.6724282005502928 1102 64-65 0.687257166118556 1102 66-67 0.7308124321085074 1102 68-69 0.679173905875956 1102 70-71 0.6318859905390077 1102 72-73 0.5572904807930001 1102 74-75 0.4198365332861691 1102 76-77 0.5856375717557931 1102 78-79 0.5567286854559015 1102 80-81 0.5394353538989094 1102 82-83 0.5104234420643792 1102 84-85 0.5299368973893195 1102 86-87 0.49234718429802626 1102 88-89 0.4716819728217061 1102 90-91 0.3474425416287552 1102 92-93 0.46293240154123794 1102 94-95 0.4587583122439973 1102 96-97 0.6136028853431057 1102 98-99 0.44509271582162313 1102 100-101 0.7835339125836533 1102 102-103 0.7454087745886753 1102 104-105 0.788546724756845 1102 106-107 0.5462563393280853 1102 108-109 0.6074597401618398 1102 110-111 0.6027497577382839 1102 112-113 0.5843289893039731 1102 114-115 0.6196275034398688 1102 116-117 0.5988404565834955 1102 118-119 0.5252180729849911 1102 120-121 0.38654278626243865 1102 122-123 0.45355859124542874 1102 124-125 0.28994340974707455 1103 1 0.5078843976017886 1103 2 0.4024388811031727 1103 3 0.6146913399240326 1103 4 0.5780855943350645 1103 5 0.5760297679021846 1103 6 0.6509562656079524 1103 7 0.5333381024263275 1103 8 0.515504452463901 1103 9 0.6449198380559267 1103 10-11 0.7440840474386832 1103 12-13 0.6548229552497915 1103 14-15 0.6376605746346371 1103 16-17 0.6004676917671148 1103 18-19 0.5898287644841673 1103 20-21 0.36396888075609724 1103 22-23 0.5669011233520109 1103 24-25 0.5125358724805409 1103 26-27 0.3451738482707256 1103 28-29 0.4413507870798554 1103 30-31 0.5860338010347945 1103 32-33 0.7392244955339464 1103 34-35 0.5078153233529576 1103 36-37 0.37951959381501865 1103 38-39 0.5271408990726982 1103 40-41 0.5503387273403817 1103 42-43 0.6914678507089533 1103 44-45 0.6452144002210858 1103 46-47 0.5798495591404276 1103 48-49 0.48948823677208964 1103 50-51 0.4791569552252213 1103 52-53 0.5306137144400509 1103 54-55 0.5466357851911425 1103 56-57 0.5094336797599226 1103 58-59 0.5133312947289355 1103 60-61 0.5832875285077534 1103 62-63 0.6079637811266281 1103 64-65 0.42065519771814763 1103 66-67 0.5864423765327729 1103 68-69 0.6074860676848601 1103 70-71 0.5526886423196835 1103 72-73 0.5439716216725117 1103 74-75 0.35595856239036294 1103 76-77 0.4066240622334263 1103 78-79 0.359489581792932 1103 80-81 0.356359888268031 1103 82-83 0.3323015579336541 1103 84-85 0.42265036118335075 1103 86-87 0.3016714074368707 1103 88-89 0.469303019884876 1103 90-91 0.3983872211409967 1103 92-93 0.35278128983378565 1103 94-95 0.3860292483793266 1103 96-97 0.4579783003103799 1103 98-99 0.35215257675505285 1103 100-101 0.3256151609594511 1103 102-103 0.19113548880355324 1103 104-105 0.2744064135728337 1103 106-107 0.3553284011669824 1103 108-109 0.4434856857859977 1103 110-111 0.32896254177639506 1103 112-113 0.5217091259337572 1103 114-115 0.35075107095933333 1103 116-117 0.3165100234906788 1103 118-119 0.24667005939141617 1103 120-121 0.32779691472898165 1103 122-123 0.32344605944572535 1103 124-125 0.24505310762390664 1104 1 0.25626407542771545 1104 2 0.3824551312963287 1104 3 0.3819742332406335 1104 4 0.35342188092436544 1104 5 0.38621041038681625 1104 6 0.2964788926023729 1104 7 0.13843348507850095 1104 8 0.16521386235335456 1104 9 0.3442138097250691 1104 10-11 0.3601642232383213 1104 12-13 0.36535555743946446 1104 14-15 0.4767880719323401 1104 16-17 0.6075835738960862 1104 18-19 0.5026461714395367 1104 20-21 0.4646901700045447 1104 22-23 0.544610405939352 1104 24-25 0.6150033481292212 1104 26-27 0.4332889677372478 1104 28-29 0.4888833689471994 1104 30-31 0.5008069444272181 1104 32-33 0.5884366602490871 1104 34-35 0.6086497274428808 1104 36-37 0.5811724892290329 1104 38-39 0.7997760415916488 1104 40-41 0.5647752486333886 1104 42-43 0.6718936149722552 1104 44-45 0.6212152652620446 1104 46-47 0.554919431520041 1104 48-49 0.39165444350231127 1104 50-51 0.423458823844058 1104 52-53 0.4830652198068677 1104 54-55 0.41591931578525987 1104 56-57 0.343196082610568 1104 58-59 0.3983659711380483 1104 60-61 0.3682447116644525 1104 62-63 0.4239598685034309 1104 64-65 0.5469636392678723 1104 66-67 0.5188227077407035 1104 68-69 0.4001948767236172 1104 70-71 0.5737655799673327 1104 72-73 0.5390747076371056 1104 74-75 0.4233491256984401 1104 76-77 0.3306641869434941 1104 78-79 0.16587665924668826 1104 80-81 0.16812778121461136 1104 82-83 0.2641715303144636 1104 84-85 0.3448133546851899 1104 86-87 0.32304627807375397 1104 88-89 0.3112238786067394 1104 90-91 0.3326837944514942 1104 92-93 0.2771350685788505 1104 94-95 0.22068468331739766 1104 96-97 0.23980162719808362 1104 98-99 0.23496560098961794 1104 100-101 0.2518757875236446 1104 102-103 0.28703559344437224 1104 104-105 0.26338193437081614 1104 106-107 0.28672348293367733 1104 108-109 0.3390128182020611 1104 110-111 0.2280549006196182 1104 112-113 0.04943875002054554 1104 114-115 0.10705588133092903 1104 116-117 0.14976992803709166 1104 118-119 0.25703329607099334 1104 120-121 0.23199401216380622 1104 122-123 0.24425646785165256 1104 124-125 0.1971760047902471 1105 1 -0.06809082128501487 1105 2 0.01369982544338555 1105 3 0.08872784711602932 1105 4 -0.06805554856674689 1105 5 -0.030315995074680302 1105 6 -0.0010479166934871387 1105 7 0.10987031005609538 1105 8 0.01920305315182702 1105 9 -0.21294054343250224 1105 10-11 0.08843012311097453 1105 12-13 0.090634888489312 1105 14-15 0.17106279868091434 1105 16-17 0.16928511672021074 1105 18-19 0.15826020601011237 1105 20-21 -0.0772126194002567 1105 22-23 -0.05720964900571346 1105 24-25 0.14653319502588147 1105 26-27 0.05954855511875223 1105 28-29 0.19816168451575322 1105 30-31 0.07415870331747243 1105 32-33 0.17124499662837422 1105 34-35 0.2043102029652104 1105 36-37 0.09741945310207001 1105 38-39 0.3145397616430543 1105 40-41 0.29937132583944503 1105 42-43 0.3464377537733725 1105 44-45 0.28559022104659704 1105 46-47 0.2695286554506211 1105 48-49 0.2980942014375074 1105 50-51 0.11234810985310872 1105 52-53 0.07647734014361873 1105 54-55 0.11795477362141327 1105 56-57 0.019383746207701336 1105 58-59 0.05483428487903552 1105 60-61 0.19163405367973496 1105 62-63 0.10481484038240296 1105 64-65 0.10363088564810141 1105 66-67 0.15107017124822164 1105 68-69 0.28850740914826645 1105 70-71 0.15129275035595313 1105 72-73 0.24840847673817734 1105 74-75 0.26042277830656246 1105 76-77 0.243577822396027 1105 78-79 0.25119680114877596 1105 80-81 0.2729215088121215 1105 82-83 0.1741020973332823 1105 84-85 0.1756478400764081 1105 86-87 0.28715549905663096 1105 88-89 0.29832457632640086 1105 90-91 0.3539527112910932 1105 92-93 0.3595677579388479 1105 94-95 0.32971719787901677 1105 96-97 0.24283406954114994 1105 98-99 0.34983179032603573 1105 100-101 0.32231964423024095 1105 102-103 0.323935774138139 1105 104-105 0.3951947969374352 1105 106-107 0.3922753211169514 1105 108-109 0.25144671611745295 1105 110-111 0.31052572579766036 1105 112-113 0.2175053266842255 1105 114-115 0.2646474515096813 1105 116-117 0.20319212949443255 1105 118-119 0.08286406736515062 1105 120-121 -0.012188525389841942 1105 122-123 0.0811217551930703 1105 124-125 0.08426413795536192 1106 1 -0.0111799429842101 1106 2 -0.18996980716281442 1106 3 -0.05341839283804717 1106 4 -0.012817675432415854 1106 5 0.026528867152649127 1106 6 0.10476974978183051 1106 7 0.11074861085977261 1106 8 -0.039133987605922016 1106 9 0.08860796403018156 1106 10-11 0.19077871668158508 1106 12-13 -0.05863265599978007 1106 14-15 -0.12866614075651484 1106 16-17 0.029013517983123904 1106 18-19 -0.09600715908747759 1106 20-21 -0.1841466392050748 1106 22-23 -0.1443874618644898 1106 24-25 -3.8537802019316345 1106 26-27 -1.0495202235838903 1106 28-29 -0.6640606174360215 1106 30-31 -0.3674061287720818 1106 32-33 -5.23411611846921 1106 34-35 -1.036083381994601 1106 36-37 -0.3835758109622276 1106 38-39 0.05435660291744426 1106 40-41 -0.5794095294992516 1106 42-43 -0.23641170087645946 1106 44-45 -0.3033459873345734 1106 46-47 -0.35653549495121695 1106 48-49 -0.19814395872322166 1106 50-51 -0.12674076069797735 1106 52-53 -0.146138546767979 1106 54-55 -0.2426382946908774 1106 56-57 -0.16959892457300896 1106 58-59 -0.01298664652165371 1106 60-61 -0.037232012623363175 1106 62-63 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-1.057885616670049 2216 48-49 -1.1431580452849204 2216 50-51 -1.141758950141714 2216 52-53 -1.5335463458908762 2216 54-55 -1.283704496972078 2216 56-57 -1.3410182482356987 2216 58-59 -1.3150384522456307 2216 60-61 -1.556104722987243 2216 62-63 -1.730902943380105 2216 64-65 -1.577371974884766 2216 66-67 -1.5342611175187564 2216 68-69 -1.4436267422073996 2216 70-71 -1.5280553550549776 2216 72-73 -1.6961303357588875 2216 74-75 -1.5239335357176849 2216 76-77 -1.2699535406743152 2216 78-79 -1.2996953165266127 2216 80-81 -1.5214510755941575 2216 82-83 -1.23620338349469 2216 84-85 -1.4876119706115283 2216 86-87 -1.5229549721383293 2216 88-89 -1.3764214192212734 2216 90-91 -1.3412883855206168 2216 92-93 -1.4209359043655532 2216 94-95 -1.3016754983641086 2216 96-97 -1.55191315215896 2216 98-99 -1.4448658713205056 2216 100-101 -1.4608505325119197 2216 102-103 -1.3798270821068677 2216 104-105 -1.4523856445361787 2216 106-107 -1.3992721566801443 2216 108-109 -1.5351150202906112 2216 110-111 -1.4793387292913884 2216 112-113 -1.4383812329229357 2216 114-115 -1.4864652634708193 2216 116-117 -1.4170769045030305 2216 118-119 -1.4817147015484338 2216 120-121 -1.453275021266446 2216 122-123 -1.2890312715678895 2216 124-125 -1.1662810226338927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33727.0 3 5915.0 4 4279.0 5 3994.0 6 4446.0 7 4065.0 8 3996.0 9 4145.0 10 5217.0 11 6058.0 12 6378.0 13 6369.0 14 6822.0 15 6311.0 16 5891.0 17 5156.0 18 4509.0 19 4137.0 20 3858.0 21 4242.0 22 4805.0 23 6990.0 24 10421.0 25 15298.0 26 18428.0 27 16079.0 28 18106.0 29 22350.0 30 25703.0 31 31137.0 32 39626.0 33 52349.0 34 71190.0 35 102044.0 36 166653.0 37 226936.0 38 78616.0 39 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.60694335370313 17.93098324942933 9.258834039876431 21.203239356991112 2 18.994468923027053 21.6846581836881 37.14388807793869 22.176984815346156 3 17.274075331859795 31.395735870572995 28.741436382002245 22.58875241556497 4 10.539370808023897 16.839684169091484 35.44742712039703 37.17351790248759 5 10.378215876924028 45.586870403994986 32.96185810295823 11.073055616122751 6 29.461292292611958 37.44161837107416 19.608798464832095 13.488290871481787 7 28.866818845767188 35.91256326592044 20.23563962496792 14.984978263344454 8 28.288248653634273 34.549741725470675 19.042560786651926 18.119448834243133 9 28.76087325661272 15.053719869405144 17.643330168800798 38.542076705181344 10-11 23.961639764976024 29.123966463738192 27.656803249428357 19.257590521857423 12-13 24.69514813017433 27.864507707284318 27.1465054554471 20.293838707094253 14-15 22.697427293334677 28.857287164735524 24.349563159415577 24.09572238251422 16-17 22.255518678604506 31.016903365236182 25.307145338067304 21.420432618092004 18-19 21.759443733170215 29.631175101135476 26.94077363695041 21.668607528743895 20-21 23.442184595776748 28.38697103790683 26.81245239274831 21.358391973568118 22-23 23.20144310414173 28.781014450837745 26.35060458473336 21.666937860287163 24-25 22.318455777856464 28.844563779627837 26.80748261187111 22.029497830644594 26-27 22.088356900865854 28.723590542885386 26.686087992208314 22.501964564040446 28-29 22.401920324479267 28.665261606100966 26.808227563853638 22.124590505566132 30-31 22.343582141535727 28.58442567712612 26.836611289302546 22.235380892035607 32-33 21.96687274720016 28.835066107856534 26.847440587831215 22.350620557112094 34-35 22.517812435178172 28.61292143232722 27.039107762217856 21.83015837027675 36-37 22.52929687028914 28.411102154700213 26.56585639287546 22.493744582135193 38-39 22.322865849393462 28.732782727952028 26.677153928791782 22.267197493862735 40-41 22.394121330293597 28.560588445845752 27.113931304890627 21.931358918970027 42-43 21.872999563431478 28.658190966167147 26.87814914242023 22.590660327981148 44-45 22.17909062491558 28.646723756671545 27.09292498157246 22.08126063684042 46-47 22.699260724321327 28.47019380359146 26.523522956137917 22.307022515949303 48-49 22.07808371032211 28.708503496854327 26.62380137828834 22.58961141453522 50-51 22.42197720773532 28.604031676076612 26.855344759052652 22.118646357135415 52-53 22.279707764407362 28.420080222481108 26.85716490953866 22.443047103572866 54-55 22.043065476176118 28.888997160131076 26.944987006646944 22.122950357045863 56-57 22.32259278287108 29.106964112137813 26.29816424141998 22.27227886357113 58-59 22.141046099461843 28.554565469494392 26.763727474630922 22.540660956412843 60-61 22.11724786335506 28.93024723190093 26.858267249334656 22.09423765540935 62-63 21.84792550256056 28.82120653126122 27.15872384929165 22.172144116886574 64-65 21.87660698142679 28.884710268183188 26.889279522389444 22.349403228000575 66-67 22.02890678665977 29.160620701897916 26.453732933013285 22.35673957842903 68-69 22.318357210839693 28.80225912935339 26.739848461971498 22.139535197835418 70-71 22.14741919922817 28.556970574047273 26.890014471780027 22.405595754944525 72-73 21.837628297802855 29.121436520536093 26.45468495312852 22.586250228532535 74-75 21.728194839670103 29.29753734550824 26.575308235813967 22.398959579007695 76-77 21.699213605749332 29.045116126050768 27.04840217539168 22.20726809280822 78-79 22.0803778856618 28.989999073809653 26.701006460177673 22.22861658035087 80-81 21.91876639223467 29.16615412668252 26.4003677745731 22.514711706509715 82-83 21.80378116479789 29.099156203630272 26.599074197351484 22.49798843422035 84-85 21.656752726835943 29.01958362899884 26.73169941056639 22.591964233598823 86-87 21.63975168462848 29.275608547768 26.6591220781 22.42551768950352 88-89 22.023787701326192 28.960773833528542 26.641579080406984 22.373859384738285 90-91 21.909080910445113 28.9942441129026 26.689911471639277 22.406763505013004 92-93 21.734996553316027 28.99343803470032 26.732905388163186 22.53866002382047 94-95 21.601170858964363 28.847211395228577 27.024497734692776 22.527120011114285 96-97 21.875758258413985 28.947659125125963 26.781482165762107 22.395100450697946 98-99 21.51348258833665 29.175637130024164 26.65630169121474 22.65457859042444 100-101 21.28124210083531 29.239210344022492 26.873127114081786 22.60642044106041 102-103 21.254282453301844 28.8827657002384 26.982084849256676 22.88086699720308 104-105 21.01273272375127 29.247300416545063 26.879240522815913 22.860726336887755 106-107 21.14448404344613 29.353681903170468 26.835934711172772 22.665899342210626 108-109 21.227834056922333 29.55103714423541 26.489339122045347 22.73178967679691 110-111 21.195961010982185 29.748228890194568 26.492647832072834 22.56316226675041 112-113 21.155982589542628 29.760053873431204 26.69653310384384 22.387430433182328 114-115 20.974468959568856 30.084814033340955 26.39924824144262 22.54146876564757 116-117 20.9937009104688 29.86254690067236 26.631429456275402 22.51232273258344 118-119 21.03449856125346 29.607546605498836 26.593921326010562 22.76403350723715 120-121 20.906888129817595 29.833897812880938 26.58078794945106 22.678426107850406 122-123 21.004562465448657 29.78988925239821 26.419221978560557 22.78632630359257 124-125 21.10802273797303 29.648173089539455 26.266661778439833 22.977142394047682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1108.0 1 1386.0 2 1749.0 3 1622.0 4 1186.5 5 860.5 6 714.5 7 644.0 8 598.5 9 592.5 10 563.5 11 515.0 12 511.0 13 529.5 14 563.0 15 605.0 16 655.0 17 792.5 18 1000.0 19 1153.5 20 1415.5 21 1833.5 22 2457.0 23 3377.0 24 4058.5 25 4954.5 26 6653.5 27 8107.5 28 9203.5 29 11071.0 30 13436.0 31 15487.5 32 18484.0 33 21569.0 34 23673.0 35 25140.5 36 28465.5 37 32151.0 38 32182.0 39 32491.5 40 32864.0 41 32938.5 42 32678.5 43 32621.5 44 34884.5 45 36216.0 46 35876.0 47 35290.5 48 34464.5 49 34153.5 50 34278.5 51 33160.5 52 31663.5 53 31526.0 54 31639.0 55 29799.5 56 27909.0 57 26269.5 58 24653.0 59 22818.0 60 19217.5 61 16446.5 62 13719.0 63 9670.5 64 6709.5 65 5306.0 66 4197.0 67 3185.0 68 2450.5 69 1786.0 70 1256.0 71 919.0 72 800.0 73 658.0 74 464.0 75 331.5 76 208.0 77 152.0 78 110.0 79 58.0 80 35.5 81 20.5 82 18.0 83 14.5 84 11.5 85 11.0 86 9.0 87 6.5 88 4.0 89 2.5 90 1.0 91 2.0 92 4.0 93 3.0 94 1.5 95 1.0 96 1.5 97 1.5 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.8238940981585265E-4 2 0.0 3 0.0 4 2.894336458895116E-4 5 8.683009376685347E-4 6 7.718230557053642E-4 7 8.683009376685347E-4 8 0.0012542124655212168 9 0.0018330797573002404 10-11 0.0016401239933738993 12-13 0.0019295576392634106 14-15 0.0029425753998767014 16-17 0.0026049028130056043 18-19 0.0025084249310424337 20-21 0.0030390532818398716 22-23 0.09276348350758847 24-25 0.002315469167116093 26-27 0.0016883629343554844 28-29 0.0014954071704291433 30-31 0.0012059735245396317 32-33 0.0018330797573002404 34-35 0.0011577345835580464 36-37 5.306283507974379E-4 38-39 0.0011094956425764613 40-41 0.0013506903474843875 42-43 0.0013989292884659728 44-45 0.001447168229447558 46-47 0.0018813186982818255 48-49 3.859115278526821E-4 50-51 0.001447168229447558 52-53 0.0013989292884659728 54-55 0.0014954071704291433 56-57 0.0013989292884659728 58-59 8.683009376685347E-4 60-61 0.0010130177606132906 62-63 0.001447168229447558 64-65 0.0013506903474843875 66-67 2.894336458895116E-4 68-69 1.447168229447558E-4 70-71 6.753451737421937E-4 72-73 2.4119470490792632E-4 74-75 3.376725868710969E-4 76-77 3.859115278526821E-4 78-79 2.894336458895116E-4 80-81 2.4119470490792632E-4 82-83 9.647788196317052E-5 84-85 3.376725868710969E-4 86-87 2.4119470490792632E-4 88-89 4.8238940981585265E-4 90-91 2.894336458895116E-4 92-93 3.376725868710969E-4 94-95 2.894336458895116E-4 96-97 3.376725868710969E-4 98-99 3.376725868710969E-4 100-101 4.8238940981585265E-4 102-103 9.647788196317053E-4 104-105 4.3415046883426737E-4 106-107 4.8238940981585265E-4 108-109 6.753451737421937E-4 110-111 9.647788196317053E-4 112-113 3.376725868710969E-4 114-115 3.859115278526821E-4 116-117 7.718230557053642E-4 118-119 0.0013989292884659728 120-121 6.271062327606084E-4 122-123 5.788672917790232E-4 124-125 2.894336458895116E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1036507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.65890885457309 #Duplication Level Percentage of deduplicated Percentage of total 1 56.84829826121468 19.13451689711811 2 12.72166510659256 8.563947326024039 3 8.833322703197988 8.91960011249917 4 6.329374645945849 8.521593772573489 5 4.327947244237843 7.283699091060118 6 2.880212458944632 5.8166885182453925 7 1.863597164801565 4.390865297818769 8 1.309974469455311 3.527384901537125 9 0.91385782858402 2.7683511622512853 >10 3.7075863674048137 20.9605221323316 >50 0.17353243877372693 3.967515075603275 >100 0.08690280759381354 5.206498989793656 >500 0.0031548873681510755 0.6848293916692411 >1k 5.736158851183773E-4 0.2539873314747354 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1589 0.15330335443947798 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1042 0.1005299530056237 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.647788196317054E-5 0.0 4 0.0 0.0 0.0 9.647788196317054E-5 0.0 5 0.0 0.0 0.0 9.647788196317054E-5 0.0 6 0.0 0.0 0.0 9.647788196317054E-5 0.0 7 0.0 0.0 0.0 9.647788196317054E-5 0.0 8 0.0 0.0 0.0 9.647788196317054E-5 0.0 9 0.0 0.0 0.0 9.647788196317054E-5 0.0 10-11 0.0 0.0 0.0 9.647788196317054E-5 0.0 12-13 0.0 0.0 0.0 9.647788196317054E-5 0.0 14-15 0.0 0.0 0.0 9.647788196317054E-5 0.0 16-17 0.0 0.0 0.0 9.647788196317054E-5 0.0 18-19 0.0 0.0 0.0 1.9295576392634107E-4 0.0 20-21 0.0 0.0 0.0 1.9295576392634107E-4 0.0 22-23 0.0 0.0 0.0 2.4119470490792632E-4 0.0 24-25 0.0 0.0 0.0 4.8238940981585265E-4 0.0 26-27 0.0 0.0 0.0 5.788672917790232E-4 0.0 28-29 0.0 0.0 0.0 9.165398786501201E-4 0.0 30-31 0.0 0.0 0.0 0.004003832101471577 0.0 32-33 0.0 0.0 0.0 0.0108055227798751 0.0 34-35 0.0 0.0 0.0 0.020549788858155324 0.0 36-37 0.0 0.0 0.0 0.03516618797557566 0.0 38-39 0.0 0.0 0.0 0.05933389740734988 0.0 40-41 0.0 0.0 0.0 0.08914556293396958 0.0 42-43 0.0 0.0 0.0 0.12653074219469815 0.0 44-45 0.0 0.0 0.0 0.17120000154364612 0.0 46-47 0.0 0.0 0.0 0.22696421731835867 0.0 48-49 0.0 0.0 0.0 0.28663578731257966 0.0 50-51 0.0 0.0 0.0 0.3410493127398078 0.0 52-53 0.0 0.0 0.0 0.4059789273010216 0.0 54-55 0.0 0.0 0.0 0.4875027375599007 0.0 56-57 0.0 0.0 0.0 0.5884668410343586 0.0 58-59 0.0 0.0 0.0 0.7062663349113898 0.0 60-61 0.0 0.0 0.0 0.8244999792572554 0.0 62-63 0.0 0.0 0.0 0.9420582784293787 0.0 64-65 0.0 0.0 0.0 1.0776097025876332 0.0 66-67 0.0 0.0 0.0 1.250015677655819 0.0 68-69 0.0 0.0 0.0 1.4540181590669432 0.0 70-71 0.0 0.0 0.0 1.660963215877944 0.0 72-73 0.0 0.0 0.0 1.862650228121952 0.0 74-75 0.0 0.0 0.0 2.0883602329747895 0.0 76-77 0.0 0.0 0.0 2.3577264794159616 0.0 78-79 0.0 0.0 0.0 2.6747527995469396 0.0 80-81 0.0 0.0 0.0 3.0025846424577933 0.0 82-83 0.0 0.0 0.0 3.3370734592241056 0.0 84-85 0.0 0.0 0.0 3.685358613111151 0.0 86-87 0.0 0.0 0.0 4.071752530373649 0.0 88-89 0.0 0.0 0.0 4.517528583984479 0.0 90-91 0.0 0.0 0.0 4.999194409685607 0.0 92-93 0.0 0.0 0.0 5.471984270246125 0.0 94-95 0.0 0.0 0.0 5.960210591920749 0.0 96-97 0.0 0.0 0.0 6.4925755445935245 0.0 98-99 0.0 0.0 0.0 7.07819628810997 0.0 100-101 0.0 0.0 0.0 7.720208353633888 0.0 102-103 0.0 0.0 0.0 8.360483817282468 0.0 104-105 0.0 0.0 0.0 9.024010450484173 0.0 106-107 0.0 0.0 0.0 9.70639850960968 0.0 108-109 0.0 0.0 0.0 10.445901474857381 0.0 110-111 0.0 0.0 0.0 11.21999176078888 0.0 112-113 0.0 0.0 0.0 11.976330116439154 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2065 0.0 58.773487 1 GTACATG 11610 0.0 55.13805 1 TACATGG 11620 0.0 52.991425 2 ACATGGG 11545 0.0 50.759075 3 CATGGGA 8090 0.0 48.095074 4 TAGATCG 50 5.33512E-4 47.595 5 CATGGGG 3175 0.0 44.78427 4 ATGGGAT 2945 0.0 44.03952 5 GTATCAA 2850 0.0 42.376255 2 ATGGGAG 2915 0.0 40.819042 5 ATGGGGG 1505 0.0 40.321346 5 TACCTGG 855 0.0 36.183334 2 GTCGGGA 420 0.0 35.41295 2 CCGTTGC 135 5.8627847E-8 35.255558 9 GAGTACA 6525 0.0 34.73888 1 CAACGCA 3695 0.0 33.81242 6 TCAACGC 3680 0.0 33.626907 5 ATCAACG 3710 0.0 33.35499 4 AACGCAG 3795 0.0 32.921444 7 AGTACAT 6655 0.0 32.71933 2 >>END_MODULE