FastQCFastQC Report
Thu 26 May 2016
SRR936254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036507
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78920.7614034444533418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.6308688701571722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61960.5977769566438047No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.16333705416364772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.15002310645273018No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.14847946034131945No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.10197712123507126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41150.084.2398761
GTATCAA63350.054.685022
GTACATG98900.046.251891
ACATGGG99650.044.9192543
TACATGG102500.044.5994342
CATGGGA67750.043.753424
ATGGGAG25400.041.7136575
ATGGGAT25000.040.9525035
TATAACG1351.3205863E-939.682662
TCAACGC95350.037.9555133
CATGGGG29200.037.7121164
AGCGTAC957.45091E-637.5940973
ATCAACG95850.037.5091134
CAACGCA96850.037.4905854
AACGCAG99800.036.9191865
TATCAAC97800.036.882963
TACCTGG7500.034.1270832
CAGCGTA1251.2738401E-633.333432
ACGCAGA113850.032.870036
CGCAGAG115250.032.5223587
Produced by FastQC (version 0.11.5)