##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936254_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1036507 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51063620409703 31.0 31.0 34.0 30.0 34.0 2 31.307866710017397 31.0 31.0 34.0 28.0 34.0 3 31.71032708896322 31.0 31.0 34.0 30.0 34.0 4 35.5823260238474 37.0 35.0 37.0 33.0 37.0 5 35.191218197272185 37.0 35.0 37.0 33.0 37.0 6 35.413505166872966 37.0 35.0 37.0 33.0 37.0 7 35.24381311462441 37.0 35.0 37.0 32.0 37.0 8 35.325177736378045 37.0 35.0 37.0 32.0 37.0 9 36.98026930835971 39.0 37.0 39.0 33.0 39.0 10-11 36.88102829985712 39.0 37.0 39.0 32.0 39.0 12-13 36.810396842471874 39.0 37.0 39.0 32.0 39.0 14-15 37.91923305872512 40.0 37.0 41.0 32.5 41.0 16-17 37.835509552757486 40.0 37.0 41.0 32.0 41.0 18-19 37.99791221863431 40.0 37.0 41.0 33.0 41.0 20-21 38.05356789679182 40.0 37.0 41.0 33.0 41.0 22-23 37.948386745096755 40.0 37.0 41.0 32.5 41.0 24-25 37.89402049383169 40.0 37.0 41.0 32.0 41.0 26-27 37.69495189130416 40.0 37.0 41.0 32.0 41.0 28-29 37.53202149141298 40.0 36.0 41.0 32.0 41.0 30-31 37.271816784643036 39.0 36.0 41.0 31.5 41.0 32-33 36.97041505749599 39.0 35.0 41.0 31.0 41.0 34-35 36.707653686854016 39.0 35.0 41.0 30.5 41.0 36-37 36.447266636887164 39.0 35.0 41.0 30.0 41.0 38-39 36.3938444216971 39.0 35.0 41.0 30.0 41.0 40-41 36.35147953655884 39.0 35.0 41.0 30.0 41.0 42-43 36.18361959928876 39.0 35.0 41.0 30.0 41.0 44-45 35.91518291724031 39.0 35.0 41.0 29.5 41.0 46-47 35.465724302874946 39.0 35.0 41.0 26.5 41.0 48-49 35.06074392165225 39.0 35.0 41.0 24.0 41.0 50-51 34.83068469388051 39.0 34.5 41.0 24.0 41.0 52-53 34.67756416502735 39.0 34.0 41.0 23.5 41.0 54-55 34.497007738490915 38.0 34.0 40.5 23.0 41.0 56-57 34.28261651875 38.0 34.0 40.0 22.5 41.0 58-59 34.05662528087123 38.0 34.0 40.0 22.0 41.0 60-61 33.82481112042659 37.5 33.5 40.0 22.0 41.0 62-63 33.58133760794669 37.0 33.0 40.0 21.0 41.0 64-65 33.33075705229197 36.5 33.0 40.0 20.5 41.0 66-67 33.037625409186816 36.0 33.0 39.0 20.0 41.0 68-69 32.72375536296427 36.0 32.5 39.0 19.0 41.0 70-71 32.39891240483663 35.0 32.0 39.0 18.0 40.5 72-73 32.13722820974678 35.0 32.0 38.0 18.0 40.0 74-75 31.95097814100628 35.0 32.0 37.0 18.0 39.5 76-77 31.651631875134466 35.0 32.0 37.0 17.0 39.0 78-79 31.322292082928527 35.0 32.0 36.0 13.0 39.0 80-81 31.059760329645627 35.0 32.0 36.0 12.5 38.0 82-83 30.807289772283255 35.0 32.0 35.5 10.5 37.0 84-85 30.554570784374828 35.0 31.5 35.0 7.5 37.0 86-87 30.361410004949313 35.0 31.0 35.0 7.0 36.0 88-89 30.20089492883309 35.0 31.0 35.0 7.0 36.0 90-91 30.06397448352978 35.0 31.0 35.0 3.5 36.0 92-93 29.927758326764796 34.0 31.0 35.0 2.0 36.0 94-95 29.80767230708524 34.0 31.0 35.0 2.0 35.0 96-97 29.741802515564295 34.0 31.0 35.0 2.0 35.0 98-99 29.6512011013915 34.0 31.0 35.0 2.0 35.0 100-101 29.56049693827442 34.0 31.0 35.0 2.0 35.0 102-103 28.54517914495512 33.5 29.0 34.5 2.0 35.0 104-105 29.28072506987411 34.0 30.5 35.0 2.0 35.0 106-107 29.418853900649008 34.0 31.0 35.0 2.0 35.0 108-109 29.379658313933238 34.0 31.0 35.0 2.0 35.0 110-111 29.330375964658224 34.0 31.0 35.0 2.0 35.0 112-113 29.242423833124136 34.0 31.0 35.0 2.0 35.0 114-115 29.211874111800498 34.0 31.0 35.0 2.0 35.0 116-117 29.10298193837572 34.0 31.0 35.0 2.0 35.0 118-119 28.960667897081255 34.0 31.0 35.0 2.0 35.0 120-121 28.751725748113614 34.0 30.0 35.0 2.0 35.0 122-123 28.525805903867507 34.0 30.0 35.0 2.0 35.0 124-125 26.50146742858466 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22135695095417773 1101 2 0.601833903465117 1101 3 0.339707105325612 1101 4 0.23489892906377463 1101 5 0.5205044249361848 1101 6 0.33808451690559593 1101 7 0.3756134026528315 1101 8 0.3299399299596715 1101 9 0.40932795142381195 1101 10-11 0.41092126553300545 1101 12-13 0.41088377192826187 1101 14-15 0.4646394718053344 1101 16-17 0.44160828470730706 1101 18-19 0.4063277523023814 1101 20-21 0.33113814196071445 1101 22-23 0.2924410334100145 1101 24-25 0.14382352181963398 1101 26-27 0.34842825128441035 1101 28-29 0.43459108899623544 1101 30-31 0.31423011088456576 1101 32-33 0.29689149643569834 1101 34-35 0.25255065634654983 1101 36-37 0.2608628899693173 1101 38-39 0.25938413688438544 1101 40-41 0.26083844477159346 1101 42-43 0.3773943300389888 1101 44-45 0.41998673934389785 1101 46-47 0.2667530208162603 1101 48-49 0.23556023010930005 1101 50-51 0.06919146027645695 1101 52-53 0.11015426498755687 1101 54-55 0.009720883285076809 1101 56-57 -0.047009926872931374 1101 58-59 0.015401013307474898 1101 60-61 -0.07134350876442852 1101 62-63 -0.32643944333815256 1101 64-65 -0.2310598805227997 1101 66-67 -0.1840884984615485 1101 68-69 -0.11595977834436866 1101 70-71 -0.3187046631273418 1101 72-73 -0.24383212134027232 1101 74-75 -0.2144660919985668 1101 76-77 -0.1678672251055815 1101 78-79 -0.13883549736887346 1101 80-81 -0.26762222226719956 1101 82-83 -0.24964160612957542 1101 84-85 -0.25799907064901006 1101 86-87 -0.10984340678582072 1101 88-89 -0.17800579483603585 1101 90-91 -0.18252107555406383 1101 92-93 -0.09899963414296309 1101 94-95 -0.061711749049603526 1101 96-97 -0.12456001717909615 1101 98-99 -0.08908612146791128 1101 100-101 -0.09436857649879116 1101 102-103 -0.0630041677708455 1101 104-105 -0.10768318229479945 1101 106-107 -0.13418127465562435 1101 108-109 -0.22224477271908682 1101 110-111 -0.1458811502996724 1101 112-113 -0.19977885608822277 1101 114-115 -0.143683094837602 1101 116-117 -0.23212137578639158 1101 118-119 -0.28055684384850466 1101 120-121 -0.12867602887907026 1101 122-123 -0.10647235585110337 1101 124-125 0.04683456032998734 1102 1 0.19455647600327097 1102 2 0.6685226431707072 1102 3 0.3556704793037859 1102 4 0.26410460411819514 1102 5 0.4746145770118062 1102 6 0.32544275471595085 1102 7 0.42539800023849494 1102 8 0.32543779115831484 1102 9 0.382309790640754 1102 10-11 0.4296704092513153 1102 12-13 0.3794658455760995 1102 14-15 0.431469022276481 1102 16-17 0.5247939570216218 1102 18-19 0.5214192412430307 1102 20-21 0.4111080008454948 1102 22-23 0.47369455367915236 1102 24-25 0.3031284494352917 1102 26-27 0.43065108241805916 1102 28-29 0.5212407061431747 1102 30-31 0.33782809234320865 1102 32-33 0.43895243324591604 1102 34-35 0.4634197251687553 1102 36-37 0.3900636176184591 1102 38-39 0.30081523094597884 1102 40-41 0.23942683587539904 1102 42-43 0.12115070458040123 1102 44-45 0.15568341012072295 1102 46-47 0.17151775266689384 1102 48-49 0.12943724522552458 1102 50-51 0.1663767672439178 1102 52-53 0.19301036399654947 1102 54-55 0.12745314493431437 1102 56-57 0.3050325903668707 1102 58-59 0.2492648607142698 1102 60-61 0.23072793176775974 1102 62-63 0.2897454221685294 1102 64-65 0.27095295228534866 1102 66-67 0.1801288220237538 1102 68-69 0.1585378697352695 1102 70-71 0.24128909553061106 1102 72-73 0.19740678505482379 1102 74-75 0.3292788654537482 1102 76-77 0.2550514395516785 1102 78-79 0.15592177531688378 1102 80-81 0.056282117748935434 1102 82-83 0.07702224432178184 1102 84-85 0.15775879572538187 1102 86-87 0.13088363344800413 1102 88-89 0.21066274423324316 1102 90-91 0.2155821653769614 1102 92-93 0.27079988177245795 1102 94-95 0.09857200367146746 1102 96-97 -0.02083271815792287 1102 98-99 0.13554586769346244 1102 100-101 0.06319220047217655 1102 102-103 0.11580571486148017 1102 104-105 0.13960487847455738 1102 106-107 0.1169916409644749 1102 108-109 0.03618180017522121 1102 110-111 0.10327284339809495 1102 112-113 0.16174973991427422 1102 114-115 0.04426236192207611 1102 116-117 0.06240831625665777 1102 118-119 0.09617855369453565 1102 120-121 0.29777241915795116 1102 122-123 0.1596147032589741 1102 124-125 0.018996271979990098 1103 1 0.1296989702718072 1103 2 0.6557576521043131 1103 3 0.3773104685387345 1103 4 0.22120171089500928 1103 5 0.4486157445109171 1103 6 0.3485922416409579 1103 7 0.3444179795874902 1103 8 0.25531574354686626 1103 9 0.33531062578047255 1103 10-11 0.3075337316576565 1103 12-13 0.2437972826873036 1103 14-15 0.4123730089697659 1103 16-17 0.48414412886916836 1103 18-19 0.3851437947363081 1103 20-21 0.38478625536492217 1103 22-23 0.31171639069349766 1103 24-25 0.2939972415055436 1103 26-27 0.24362084850952925 1103 28-29 0.32015729935919524 1103 30-31 0.23189914013895674 1103 32-33 0.2762912370142985 1103 34-35 0.28206215658975253 1103 36-37 0.31000761004899857 1103 38-39 0.2560301888149894 1103 40-41 0.3495883579117489 1103 42-43 0.4038703683036431 1103 44-45 0.3676727931275181 1103 46-47 0.38679795630834946 1103 48-49 0.542396099750043 1103 50-51 0.6661814771851695 1103 52-53 0.43496838492362855 1103 54-55 0.6572517474826043 1103 56-57 0.6603783713690277 1103 58-59 0.7810763196331934 1103 60-61 0.6410838185002916 1103 62-63 0.45639705169346456 1103 64-65 0.41526247372638636 1103 66-67 0.5143260198716675 1103 68-69 0.4301819074314537 1103 70-71 0.4505237898409433 1103 72-73 0.5072686824028096 1103 74-75 0.5783604431043905 1103 76-77 0.4661366662153874 1103 78-79 0.4113451711044789 1103 80-81 0.5635755170606203 1103 82-83 0.45147656958209836 1103 84-85 0.5654704589180817 1103 86-87 0.6235385680595691 1103 88-89 0.5970636930383293 1103 90-91 0.6324593520279898 1103 92-93 0.5377698745777657 1103 94-95 0.625566179285233 1103 96-97 0.5581606647285753 1103 98-99 0.5722913598282666 1103 100-101 0.6044009090593114 1103 102-103 0.6653816289076353 1103 104-105 0.6444663092855833 1103 106-107 0.6033259636646235 1103 108-109 0.5638634430639264 1103 110-111 0.613286037526052 1103 112-113 0.6717386432748036 1103 114-115 0.6027840258165114 1103 116-117 0.5987821982066137 1103 118-119 0.5525072239716557 1103 120-121 0.5990641096334244 1103 122-123 0.6086754216950503 1103 124-125 0.831771326364315 1104 1 0.08519077251332163 1104 2 0.33998856129073474 1104 3 0.27680658984998985 1104 4 0.14357080347241435 1104 5 0.3630039140021921 1104 6 0.21099858159362128 1104 7 0.36097722549877176 1104 8 0.33474460178866394 1104 9 0.28392696286116603 1104 10-11 0.2550421184521383 1104 12-13 0.16837652423503613 1104 14-15 0.07505419881519515 1104 16-17 0.2664699540611011 1104 18-19 0.2849182935896337 1104 20-21 0.2493852977615134 1104 22-23 0.31096503973802214 1104 24-25 0.3099236194647901 1104 26-27 0.19622707197307676 1104 28-29 0.269509074629525 1104 30-31 0.1421072369376546 1104 32-33 0.06042651622966133 1104 34-35 0.040204220394961965 1104 36-37 0.29311285401470144 1104 38-39 0.31662312531078385 1104 40-41 0.23155397943562406 1104 42-43 0.2019195399003948 1104 44-45 0.281513410098043 1104 46-47 0.16859616786684484 1104 48-49 0.09486547342741858 1104 50-51 -0.026308157469230764 1104 52-53 -0.07828273117475959 1104 54-55 0.13620126720466175 1104 56-57 0.09113924314318922 1104 58-59 0.0013295128268211442 1104 60-61 0.039036209805559 1104 62-63 0.04724859172326745 1104 64-65 -0.03228214816137864 1104 66-67 -0.05016286017254146 1104 68-69 0.07260154508819738 1104 70-71 0.06967934728813674 1104 72-73 -0.09236536232701553 1104 74-75 -0.03972742527134443 1104 76-77 0.009543815610179252 1104 78-79 -0.0703010831263029 1104 80-81 -0.08672055624393238 1104 82-83 -0.08798114636467957 1104 84-85 0.058356493989816016 1104 86-87 0.036708356504142614 1104 88-89 0.09119226588563123 1104 90-91 0.06562669412446454 1104 92-93 0.043079753644580165 1104 94-95 0.029423357035430087 1104 96-97 0.06802197826413092 1104 98-99 0.051129681909550584 1104 100-101 0.05422105646136899 1104 102-103 0.0581871032058352 1104 104-105 -0.06365694678072487 1104 106-107 -0.0571036656752959 1104 108-109 -0.0866387293161921 1104 110-111 0.01963250866892352 1104 112-113 -0.006678546500072713 1104 114-115 -0.1441972458167058 1104 116-117 -0.10413279043013901 1104 118-119 -0.025739871756858435 1104 120-121 -0.13766032995922117 1104 122-123 -0.19935239612026834 1104 124-125 -0.11922636570947631 1105 1 0.18809484269207033 1105 2 0.5523873816227614 1105 3 0.2523495554680615 1105 4 0.12983601358418895 1105 5 0.3465060275002827 1105 6 0.20640865033389133 1105 7 0.3416672369358338 1105 8 0.14625343898151755 1105 9 0.23437840251592945 1105 10-11 0.17777489801163426 1105 12-13 0.22331328878485834 1105 14-15 0.20282798082718045 1105 16-17 0.39917891623627355 1105 18-19 0.35910701393580524 1105 20-21 0.22094850520857534 1105 22-23 0.23946664475853652 1105 24-25 0.22353327965439007 1105 26-27 0.1137765122055896 1105 28-29 0.06530319132291851 1105 30-31 0.08139980461675123 1105 32-33 0.033852114906729014 1105 34-35 -0.07398068268479108 1105 36-37 -0.03787583272774242 1105 38-39 -0.11235434839576186 1105 40-41 -0.046584543120140154 1105 42-43 -0.0882291861053659 1105 44-45 -0.08836662604070966 1105 46-47 -0.1505398329349319 1105 48-49 -0.17693531832374987 1105 50-51 -0.1351592044908756 1105 52-53 -0.36272011179306674 1105 54-55 -0.2975759768763666 1105 56-57 -0.26198277231453204 1105 58-59 -0.18700431670202278 1105 60-61 -0.2608763910056524 1105 62-63 -0.21679957510453818 1105 64-65 -0.1788808385000067 1105 66-67 -0.21339687334284463 1105 68-69 -0.13598737805947536 1105 70-71 -0.14663898767625483 1105 72-73 -0.25394682338205854 1105 74-75 -0.31032242509448693 1105 76-77 -0.3608181688377776 1105 78-79 -0.22680513768226263 1105 80-81 -0.11996448841155072 1105 82-83 -0.13295119777249909 1105 84-85 -0.2054728064039928 1105 86-87 -0.22937243100234284 1105 88-89 -0.3425201288816204 1105 90-91 -0.26279989203079523 1105 92-93 -0.33011386135094156 1105 94-95 -0.2816243499517874 1105 96-97 -0.24691281869856496 1105 98-99 -0.43087223851444634 1105 100-101 -0.2566890299650808 1105 102-103 -0.28076146834064275 1105 104-105 -0.2851308021903378 1105 106-107 -0.234028437523623 1105 108-109 -0.2025970400505237 1105 110-111 -0.22862687679539562 1105 112-113 -0.24618124806353237 1105 114-115 -0.22051862427729674 1105 116-117 -0.26547622502666 1105 118-119 -0.3440028018257131 1105 120-121 -0.3676264977774544 1105 122-123 -0.3828842445025735 1105 124-125 -0.3277937338295551 1106 1 0.2025896762167534 1106 2 0.5580435957444614 1106 3 0.2848093717711606 1106 4 0.14129841312495017 1106 5 0.3429422358814591 1106 6 0.21904821692402265 1106 7 0.2607727908967945 1106 8 0.24885461578978152 1106 9 0.12715179516805364 1106 10-11 0.16423801511840708 1106 12-13 0.17975147047257423 1106 14-15 0.0654944561429005 1106 16-17 0.09160644206864532 1106 18-19 -0.08059411981849252 1106 20-21 -0.00867943681209482 1106 22-23 0.07635940451744005 1106 24-25 0.07101534050399039 1106 26-27 0.16383086783130807 1106 28-29 0.12131119218399533 1106 30-31 0.10159605892215495 1106 32-33 0.09040080176092147 1106 34-35 0.1879624860867395 1106 36-37 0.22026495372460886 1106 38-39 0.24373276411859024 1106 40-41 0.2907997493597705 1106 42-43 -0.06051427508354834 1106 44-45 0.20909121695584076 1106 46-47 0.06438338750135131 1106 48-49 0.15743142966706358 1106 50-51 0.2367739183564197 1106 52-53 0.17749533740325774 1106 54-55 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-0.7101653194891995 2216 64-65 -0.830937140189306 2216 66-67 -0.6726564901959051 2216 68-69 -0.41299760725453183 2216 70-71 -0.34870835853524085 2216 72-73 -0.49422259020457204 2216 74-75 -0.42927261099974956 2216 76-77 -0.239779485563961 2216 78-79 -0.34832326858541407 2216 80-81 -0.41107418573545473 2216 82-83 -0.48714038061165255 2216 84-85 -0.4442826969753959 2216 86-87 -0.42419667269681227 2216 88-89 -0.38978151374370285 2216 90-91 -0.3096442382198852 2216 92-93 -0.3050619884948702 2216 94-95 -0.45781309372557644 2216 96-97 -0.4652208164430327 2216 98-99 -0.5431171327275948 2216 100-101 -0.6850585996516578 2216 102-103 -0.7595516009706884 2216 104-105 -0.46711476822811804 2216 106-107 -0.26923259557540646 2216 108-109 -0.4179215225701398 2216 110-111 -0.4278994086313084 2216 112-113 -0.47977595255345307 2216 114-115 -0.5445969541255167 2216 116-117 -0.506445832599141 2216 118-119 -0.47401993124867303 2216 120-121 -0.5312754466637841 2216 122-123 -0.6606615531934246 2216 124-125 -0.8330105719128191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 49.0 4 142.0 5 264.0 6 768.0 7 1487.0 8 2321.0 9 3948.0 10 6490.0 11 8415.0 12 9175.0 13 11749.0 14 16100.0 15 12420.0 16 5911.0 17 4856.0 18 4714.0 19 4441.0 20 4261.0 21 4491.0 22 4791.0 23 5655.0 24 6753.0 25 8618.0 26 10703.0 27 13443.0 28 17056.0 29 21027.0 30 26581.0 31 32703.0 32 41551.0 33 52596.0 34 70341.0 35 100258.0 36 166082.0 37 255300.0 38 100642.0 39 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.5839254270798 17.949321923944268 11.230827747057551 21.235924901918384 2 19.177212564194342 21.83683373195009 36.45659772638907 22.529355977466494 3 17.792275201494252 28.805718442536755 32.101406070008274 21.30060028596072 4 11.283377729238683 16.30727047670686 36.67037463326345 35.738977160791 5 9.965997034153576 42.86961088712792 34.81895979115498 12.345432287563526 6 29.57847848591471 37.25377638549474 18.25467652413346 14.913068604457086 7 26.335856873132553 34.938693129906504 22.355179463332135 16.370270533628815 8 28.67872575872618 32.13620361464032 20.310909622414513 18.87416100421898 9 27.993925752551597 10.949950169173967 19.729051516294632 41.327072561979804 10-11 24.60624964423781 25.8424689847729 29.325561718348258 20.225719652641033 12-13 26.16668290711013 25.402047453611022 29.500717313052395 18.93055232622645 14-15 23.130234528083264 26.21617606055724 28.218960412230693 22.434628999128805 16-17 20.712219363795594 28.32471383440432 29.130352776369467 21.832714025430615 18-19 20.177625428482393 25.43417458830476 33.27758519720562 21.110614786007233 20-21 21.3852390770154 24.558879004193894 34.09803310542042 19.95784881337029 22-23 21.112833777292387 25.037650493436125 33.64333284772799 20.20618288154349 24-25 20.580179390973722 25.186805298951185 33.89571898694365 20.33729632313144 26-27 20.3481500848523 25.263746409816818 33.73913538451742 20.648968120813464 28-29 20.489200748282453 25.02814742206276 33.81998384960256 20.662667980052234 30-31 20.44347023223191 24.860468863210762 33.94936068931517 20.746700215242157 32-33 20.38510111364419 25.190037307996953 33.613231748555485 20.81162982980337 34-35 20.896685163093526 25.106644290219116 33.52265518836592 20.474015358321438 36-37 21.063533579609206 24.928099858466947 32.75713526295529 21.251231298968555 38-39 20.990982212372902 25.343678335023306 32.71511914536033 20.950220307243463 40-41 20.92581140310678 25.424092649639608 32.83484819687663 20.815247750376987 42-43 20.827769652015853 25.319783035724626 32.36769694467064 21.484750367588887 44-45 20.966812087611498 25.503212270356563 32.15261432059408 21.377361321437856 46-47 21.704547866746175 25.758588881877426 30.81099153028084 21.72587172109556 48-49 21.528603606754622 26.235832795020148 30.280587003391013 21.954976594834218 50-51 21.859892486242376 26.291228843751913 30.116732944143116 21.73214572586259 52-53 21.564916614002673 26.219722223562275 30.13493238907998 22.080428773355074 54-55 21.40983287053207 26.7017353600105 30.023725399009304 21.86470637044813 56-57 21.71856683181452 26.72626140316177 29.63731794740676 21.91785381761695 58-59 21.482356244238265 26.16728969392791 30.0644542565811 22.285899805252726 60-61 21.716195466502395 26.45167390346889 30.186220826264165 21.645909803764553 62-63 21.380342010218143 26.272383849522836 30.656509303760433 21.690764836498584 64-65 21.43386403675496 26.200739807387652 30.4711785783889 21.894217577468485 66-67 21.61152635321382 26.614772927908675 29.900904699284208 21.872796019593295 68-69 21.73106182301631 26.398396126932123 30.05057448641619 21.81996756363538 70-71 21.762459870088836 26.09820280710387 30.075037808346057 22.06429951446124 72-73 21.57966310027058 26.635619290473866 29.54088378893766 22.243833820317892 74-75 21.472693320906842 26.841650542621746 29.498487933618623 22.187168202852785 76-77 21.4168837433671 26.81056439942113 29.813458755426918 21.959093101784852 78-79 21.55653555644101 26.632285165464392 29.469651435060257 22.341527843034346 80-81 21.486813142814967 26.949977592979486 28.922277788202766 22.64093147600278 82-83 21.487236928681554 26.76467864151293 29.11155362342331 22.63653080638221 84-85 21.3418202078625 26.728059025424443 29.124145865543017 22.80597490117004 86-87 21.228262285276 27.001365164663945 29.097206477537497 22.673166072522562 88-89 21.478657860055147 26.756546588788744 28.998865405877893 22.76593014527822 90-91 21.363813096302813 26.805394354606072 28.84757320613493 22.983219342956186 92-93 21.270729025918488 26.71356812038983 29.027124470392895 22.988578383298787 94-95 21.284927843287083 26.5490683014084 29.096405767906035 23.06959808739848 96-97 21.466756585440685 26.583540040386268 28.933295127502312 23.01640824667074 98-99 21.368333611516594 26.721560424781014 28.626215282970264 23.283890680732128 100-101 21.10257217959188 26.693098709158612 28.84598036940862 23.35834874184089 102-103 21.13397610889397 26.285378262981617 28.69918750171853 23.88145812640588 104-105 20.884217818075868 26.470576884856506 28.736041941229022 23.909163355838604 106-107 21.15079317223065 26.4552183906937 28.573833637641975 23.820154799433674 108-109 21.120372563482007 26.77607068826704 28.18476123124628 23.918795517004675 110-111 21.07265351014718 26.76042817379727 28.107033801417273 24.059884514638277 112-113 20.944110058529862 26.777702302406226 28.291460583523254 23.986727055540655 114-115 20.83407716914585 26.987875074182544 28.05779242397194 24.120255332699667 116-117 20.72945670943559 26.926712248463414 28.220297971978344 24.12353307012266 118-119 20.629407740544796 26.78275586243363 28.073875878412213 24.513960518609363 120-121 20.39656653768429 26.973365243647407 27.967000708671407 24.663067509996896 122-123 20.49718146448066 27.048967984234533 27.68638877699438 24.76746177429042 124-125 20.356987794876744 26.910801292874716 27.61368131603068 25.118529596217858 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 103.0 1 94.5 2 428.5 3 844.5 4 683.5 5 629.0 6 1052.5 7 2404.5 8 3638.0 9 4000.0 10 3316.5 11 2239.0 12 2113.0 13 2191.5 14 2260.0 15 2369.0 16 2510.5 17 2676.0 18 2885.0 19 3003.5 20 3201.0 21 3570.0 22 3992.0 23 4671.0 24 5373.5 25 6216.0 26 7598.5 27 8913.0 28 10148.5 29 11770.0 30 13723.0 31 15698.0 32 18323.5 33 21199.5 34 23321.5 35 24928.5 36 27697.0 37 30643.5 38 31120.0 39 31529.5 40 31732.5 41 31760.0 42 31482.0 43 31439.0 44 33805.5 45 34989.5 46 34325.5 47 33901.5 48 33675.5 49 33225.5 50 33340.0 51 32524.5 52 30712.5 53 30105.0 54 30172.5 55 29057.0 56 26891.0 57 24584.0 58 23017.0 59 21206.5 60 18155.0 61 15510.0 62 12665.5 63 9113.0 64 6297.5 65 4993.0 66 3811.0 67 2679.0 68 2112.0 69 1642.0 70 1197.0 71 854.0 72 665.0 73 592.5 74 457.5 75 326.0 76 202.5 77 126.0 78 80.0 79 25.0 80 11.5 81 3.0 82 1.0 83 2.0 84 3.5 85 3.0 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06126345504661329 2 5.788672917790232E-4 3 9.647788196317052E-5 4 0.0 5 0.06898168560366692 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.823894098158526E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 4.823894098158526E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0022189912851529226 44-45 0.008731248317666933 46-47 0.010516089133985588 48-49 0.008731248317666933 50-51 0.007862947379998398 52-53 0.005981628681716573 54-55 0.006271062327606085 56-57 0.005981628681716573 58-59 0.0034249648096925543 60-61 0.002170752344171337 62-63 0.0016883629343554844 64-65 0.0020260355212265812 66-67 0.002894336458895116 68-69 0.0015436461114107284 70-71 0.0028460975179135308 72-73 0.002556663872024019 74-75 0.0011094956425764613 76-77 6.753451737421937E-4 78-79 0.0 80-81 1.447168229447558E-4 82-83 9.647788196317052E-5 84-85 4.3415046883426737E-4 86-87 1.9295576392634105E-4 88-89 0.0012542124655212168 90-91 0.0015436461114107284 92-93 7.23584114723779E-4 94-95 0.0012542124655212168 96-97 0.0015436461114107284 98-99 0.0019295576392634106 100-101 0.0012059735245396317 102-103 0.0012059735245396317 104-105 8.683009376685347E-4 106-107 4.3415046883426737E-4 108-109 0.0012059735245396317 110-111 0.0011577345835580464 112-113 0.01090200066183827 114-115 0.02045331097619215 116-117 0.019440293215578865 118-119 0.011577345835580463 120-121 0.005836911858771818 122-123 0.006222823386624499 124-125 0.00549923927190072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1036507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.914823345317867 #Duplication Level Percentage of deduplicated Percentage of total 1 44.48772419538638 11.973792377540477 2 15.54549645455838 8.36808581779408 3 11.372925356052043 9.183008306328812 4 8.252037297962707 8.884085044545618 5 5.691158854932071 7.658826760531909 6 3.895562728995293 6.290902960890761 7 2.5850667707201307 4.870363082884854 8 1.7347146491572158 3.7351630669281217 9 1.1945497509362262 2.8935985971277787 >10 4.85792012951049 21.911524774672696 >50 0.25125417652604864 4.6602434076592525 >100 0.1247209455153482 6.189141346322153 >500 0.004338119840674077 0.8056421312216727 >1k 0.0014460399468913588 0.5686198810216734 >5k 0.0010845299601685192 2.007002444530126 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7892 0.7614034444533418 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6539 0.6308688701571722 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6196 0.5977769566438047 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.16333705416364772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.15002310645273018 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1539 0.14847946034131945 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1057 0.10197712123507126 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.647788196317054E-5 0.0 20-21 0.0 0.0 0.0 9.647788196317054E-5 0.0 22-23 0.0 0.0 0.0 1.447168229447558E-4 0.0 24-25 0.0 0.0 0.0 3.8591152785268215E-4 0.0 26-27 0.0 0.0 0.0 4.8238940981585265E-4 0.0 28-29 0.0 0.0 0.0 8.683009376685348E-4 0.0 30-31 0.0 0.0 0.0 0.003955593160489991 0.0 32-33 0.0 0.0 0.0 0.01070904489791193 0.0 34-35 0.0 0.0 0.0 0.02064626674011849 0.0 36-37 0.0 0.0 0.0 0.03526266585753883 0.0 38-39 0.0 0.0 0.0 0.05971980893520256 0.0 40-41 0.0 0.0 0.0 0.08982090810771176 0.0 42-43 0.0 0.0 0.0 0.12744728207334827 0.0 44-45 0.0 0.0 0.0 0.17206830248131466 0.0 46-47 0.0 0.0 0.0 0.22807371296093515 0.0 48-49 0.0 0.0 0.0 0.2877935218961377 0.0 50-51 0.0 0.0 0.0 0.34288239249710806 0.0 52-53 0.0 0.0 0.0 0.4088250248189351 0.0 54-55 0.0 0.0 0.0 0.49073474660566696 0.0 56-57 0.0 0.0 0.0 0.5924224341948486 0.0 58-59 0.0 0.0 0.0 0.7107525564226773 0.0 60-61 0.0 0.0 0.0 0.8295650680603218 0.0 62-63 0.0 0.0 0.0 0.94726808405539 0.0 64-65 0.0 0.0 0.0 1.0830124639775707 0.0 66-67 0.0 0.0 0.0 1.2560455452785173 0.0 68-69 0.0 0.0 0.0 1.4614469559781074 0.0 70-71 0.0 0.0 0.0 1.6703215704283714 0.0 72-73 0.0 0.0 0.0 1.8747582023083298 0.0 74-75 0.0 0.0 0.0 2.1014812249217805 0.0 76-77 0.0 0.0 0.0 2.37258407323829 0.0 78-79 0.0 0.0 0.0 2.692794163474053 0.0 80-81 0.0 0.0 0.0 3.0215425462635563 0.0 82-83 0.0 0.0 0.0 3.358105637492077 0.0 84-85 0.0 0.0 0.0 3.7091404110150727 0.0 86-87 0.0 0.0 0.0 4.099538160379042 0.0 88-89 0.0 0.0 0.0 4.5492698071503614 0.0 90-91 0.0 0.0 0.0 5.034119402956275 0.0 92-93 0.0 0.0 0.0 5.511009573500227 0.0 94-95 0.0 0.0 0.0 6.003384444099268 0.0 96-97 0.0 0.0 0.0 6.541489830748851 0.0 98-99 0.0 0.0 0.0 7.135697105760019 0.0 100-101 0.0 0.0 0.0 7.785137968195102 0.0 102-103 0.0 0.0 0.0 8.432215122522086 0.0 104-105 0.0 0.0 0.0 9.103459986280846 0.0 106-107 0.0 0.0 0.0 9.792022629851992 0.0 108-109 0.0 0.0 0.0 10.539436781420676 0.0 110-111 0.0 0.0 0.0 11.32346428919438 0.0 112-113 0.0 0.0 0.0 12.09480495548993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4115 0.0 84.239876 1 GTATCAA 6335 0.0 54.68502 2 GTACATG 9890 0.0 46.25189 1 ACATGGG 9965 0.0 44.919254 3 TACATGG 10250 0.0 44.599434 2 CATGGGA 6775 0.0 43.75342 4 ATGGGAG 2540 0.0 41.713657 5 ATGGGAT 2500 0.0 40.952503 5 TATAACG 135 1.3205863E-9 39.68266 2 TCAACGC 9535 0.0 37.955513 3 CATGGGG 2920 0.0 37.712116 4 AGCGTAC 95 7.45091E-6 37.594097 3 ATCAACG 9585 0.0 37.509113 4 CAACGCA 9685 0.0 37.490585 4 AACGCAG 9980 0.0 36.919186 5 TATCAAC 9780 0.0 36.88296 3 TACCTGG 750 0.0 34.127083 2 CAGCGTA 125 1.2738401E-6 33.33343 2 ACGCAGA 11385 0.0 32.87003 6 CGCAGAG 11525 0.0 32.522358 7 >>END_MODULE