FastQCFastQC Report
Thu 26 May 2016
SRR936253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413606
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG86340.2529290140689933No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG50640.14834752458250894No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45720.1339346134263884No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40100.1174710848293564No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39970.11709025587604428No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39550.11585988541149742No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG38770.11357491169162463No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38010.1113485270414922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.10976662215850334No Hit
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT35500.1039955987890811No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA35390.10367335890550931No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34840.10206215948765031No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.073.4392241
GTATCAA74150.048.0916982
TACATGG151600.043.942562
GTACATG155950.043.622341
ACATGGG153600.042.9052933
TCAACGC95600.038.6089745
ATCAACG97000.037.990364
CAACGCA97300.037.971666
AACGCAG101950.036.3564647
TATCAAC105250.035.238743
CATGGGG82300.034.721294
CATGGGA78650.031.4125944
ACGCAGA119300.030.4706428
CGCAGAG123550.029.2780179
GAGTACA97150.028.9419251
CAGAGTA140650.025.2531439
ATGGGGG38800.024.5495035
ATGGGGA45100.023.8922185
AGAGTAC133300.023.58824710-11
GCAGAGT149850.023.5836248
Produced by FastQC (version 0.11.5)