FastQCFastQC Report
Thu 26 May 2016
SRR936252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288409
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20050.15561828580831086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22650.075.91072
GTACATG146550.054.5209661
TACATGG148700.052.932482
ACCTGGG32600.050.916663
GTACCTG34350.050.401051
ACATGGG154250.049.099443
CATGGGG38500.046.6680374
CATGGGA113300.044.6861534
ATGGGAT41750.042.75025
TGGGATA14100.042.6162836
ATGGGAG34250.041.862955
ATGGGGG25250.039.819765
GGGATAG7750.038.383247
GGTATCA31900.037.4868281
TATCACG801.19825905E-437.1837652
GAACGCA3950.034.642099
CCTGGGA31950.033.145344
ATGGGAC20950.032.94185
GGAACGC3800.032.8782778
GTCGGGA5100.032.6633832
Produced by FastQC (version 0.11.5)