##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936252_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1288409 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.787474319102085 31.0 30.0 33.0 18.0 34.0 2 28.914072317098064 31.0 30.0 34.0 18.0 34.0 3 29.241525012631858 31.0 30.0 34.0 22.0 34.0 4 32.393566794395255 35.0 33.0 37.0 19.0 37.0 5 32.97097815988556 35.0 35.0 37.0 26.0 37.0 6 32.69688972989167 37.0 35.0 37.0 22.0 37.0 7 32.658622378452804 37.0 33.0 37.0 22.0 37.0 8 32.5595567867036 37.0 35.0 37.0 19.0 37.0 9 33.744415011071794 39.0 35.0 39.0 17.0 39.0 10-11 33.6983710141733 38.5 34.0 39.0 17.0 39.0 12-13 33.711395993042586 38.0 34.0 39.0 17.0 39.0 14-15 34.80969474755299 39.0 34.0 41.0 17.0 41.0 16-17 34.895708971297154 39.0 34.0 41.0 18.5 41.0 18-19 34.9603130682881 39.0 34.0 41.0 20.5 41.0 20-21 35.11583976827234 39.0 34.0 41.0 23.5 41.0 22-23 35.10103546311769 39.0 34.0 41.0 24.0 41.0 24-25 35.15750394478772 39.0 34.0 41.0 25.0 41.0 26-27 35.290402348943545 39.0 34.0 41.0 26.5 41.0 28-29 35.19747727623759 39.0 34.0 41.0 26.5 41.0 30-31 35.125731813422604 39.0 34.0 41.0 26.0 41.0 32-33 34.94690389464836 39.0 34.0 41.0 25.0 41.0 34-35 34.90846928265791 39.0 34.0 41.0 25.0 41.0 36-37 34.78685223403438 39.0 33.0 41.0 25.0 41.0 38-39 34.54280628278753 38.0 33.0 40.5 24.0 41.0 40-41 34.63836173140672 38.5 33.0 40.5 24.5 41.0 42-43 34.55922187752492 39.0 33.0 41.0 24.0 41.0 44-45 34.47604603817577 39.0 33.0 41.0 24.0 41.0 46-47 34.458691688741695 39.0 33.0 41.0 24.0 41.0 48-49 34.360290870368026 38.5 33.0 40.5 24.0 41.0 50-51 34.238791408628785 38.0 33.0 40.0 23.0 41.0 52-53 34.097958412274366 38.0 33.0 40.0 23.0 41.0 54-55 33.92676704369498 38.0 32.5 40.0 23.0 41.0 56-57 33.73634924934551 38.0 32.0 40.0 22.0 41.0 58-59 33.5072236378355 38.0 32.0 40.0 21.0 41.0 60-61 33.259744382412734 37.0 31.5 40.0 19.5 41.0 62-63 32.98035872149294 37.0 31.0 40.0 18.0 41.0 64-65 32.692318200198855 36.5 31.0 40.0 16.0 41.0 66-67 32.39260359094045 36.0 31.0 39.0 13.0 41.0 68-69 32.08434006592627 35.5 31.0 39.0 10.0 41.0 70-71 31.710888390254958 35.0 31.0 39.0 7.5 40.0 72-73 31.34930289993317 35.0 30.5 38.0 4.5 40.0 74-75 30.986712682075336 35.0 30.0 37.0 2.0 39.5 76-77 30.631127227456496 35.0 30.0 37.0 2.0 39.0 78-79 30.26839613818283 35.0 30.0 36.0 2.0 39.0 80-81 29.942185284331295 34.0 30.0 36.0 2.0 37.5 82-83 29.63246414764256 34.0 29.0 35.5 2.0 37.0 84-85 29.64710274454773 34.0 30.0 35.0 2.0 37.0 86-87 29.505632528180104 34.0 30.0 35.0 2.0 36.5 88-89 29.327698735417094 34.0 30.0 35.0 2.0 36.0 90-91 29.149248414129367 34.0 29.5 35.0 2.0 36.0 92-93 29.014254790210252 34.0 29.0 35.0 2.0 36.0 94-95 28.88504116317101 34.0 29.0 35.0 2.0 35.0 96-97 28.762613036698752 34.0 29.0 35.0 2.0 35.0 98-99 28.648102427101954 34.0 29.0 35.0 2.0 35.0 100-101 28.53457636511387 34.0 29.0 35.0 2.0 35.0 102-103 27.589645446438205 32.5 26.5 34.5 2.0 35.0 104-105 28.223849336662504 34.0 28.5 35.0 2.0 35.0 106-107 28.320563190725927 34.0 29.0 35.0 2.0 35.0 108-109 28.253863097820645 34.0 29.0 35.0 2.0 35.0 110-111 28.128347442465863 34.0 29.0 35.0 2.0 35.0 112-113 28.063238847291505 34.0 28.0 35.0 2.0 35.0 114-115 27.941672248486313 34.0 28.0 35.0 2.0 35.0 116-117 27.8182952773537 34.0 28.0 35.0 2.0 35.0 118-119 27.614064710817757 34.0 27.0 35.0 2.0 35.0 120-121 27.399429451362106 34.0 27.0 35.0 2.0 35.0 122-123 27.10022477334449 34.0 27.0 35.0 2.0 35.0 124-125 25.17490059445409 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6178450454368871 1101 2 0.5939079022452276 1101 3 0.4433332886240393 1101 4 0.5453498255449958 1101 5 0.4952641152354218 1101 6 0.35350431186191145 1101 7 0.3760852968720769 1101 8 0.520063021407708 1101 9 0.44480234935638663 1101 10-11 0.6553982416424375 1101 12-13 0.6149412592231371 1101 14-15 0.564685004045721 1101 16-17 0.5432548098753927 1101 18-19 0.5008189236701668 1101 20-21 0.3411135263512648 1101 22-23 0.42325378631201716 1101 24-25 0.5862869475731074 1101 26-27 0.544872538170253 1101 28-29 0.5110461873933829 1101 30-31 0.5068342592136901 1101 32-33 0.6278463807017545 1101 34-35 0.4573800826007215 1101 36-37 0.41676766348948746 1101 38-39 0.6161647985892529 1101 40-41 0.5543122757335297 1101 42-43 0.6307486500701174 1101 44-45 0.577544994602853 1101 46-47 0.4900366002489136 1101 48-49 0.4958684989983482 1101 50-51 0.26003619319711646 1101 52-53 0.47488215153910573 1101 54-55 0.4979708054034333 1101 56-57 0.34167274717571416 1101 58-59 0.4231811761261639 1101 60-61 0.5306181142880533 1101 62-63 0.5480430170298263 1101 64-65 0.5106885074857956 1101 66-67 0.3648722631132202 1101 68-69 0.3039925171296147 1101 70-71 0.42315417704908853 1101 72-73 0.45344737450438544 1101 74-75 0.40497107458840986 1101 76-77 0.30739130531538095 1101 78-79 0.3529073888216878 1101 80-81 0.5239785163672472 1101 82-83 0.509714599447765 1101 84-85 0.3854757152653292 1101 86-87 0.4067218583616885 1101 88-89 0.36542444613958125 1101 90-91 0.4526202923349203 1101 92-93 0.39410595644436697 1101 94-95 0.4097554970682147 1101 96-97 0.5339440111963434 1101 98-99 0.5130592230120463 1101 100-101 0.5325121388564078 1101 102-103 0.5783671557842816 1101 104-105 0.6229839558611623 1101 106-107 0.4194911797920078 1101 108-109 0.4785093319371079 1101 110-111 0.564271089189404 1101 112-113 0.4349901736317605 1101 114-115 0.4535169214758028 1101 116-117 0.5900934785263487 1101 118-119 0.45880242822322614 1101 120-121 0.6137793247947698 1101 122-123 0.5804122781629957 1101 124-125 0.5090004053903563 1102 1 0.29227720901798904 1102 2 0.3479283498826966 1102 3 0.3702094975215253 1102 4 0.5132069684021374 1102 5 0.34551832910666036 1102 6 0.3441329385543668 1102 7 0.2540888890246009 1102 8 0.4470290341184011 1102 9 0.43711928773716124 1102 10-11 0.36830097720474697 1102 12-13 0.4411881506296069 1102 14-15 0.45999102780919543 1102 16-17 0.44879294195607855 1102 18-19 0.5404141157121671 1102 20-21 0.3667497518279177 1102 22-23 0.49809559344106447 1102 24-25 0.595204466224672 1102 26-27 0.4743468404637028 1102 28-29 0.5360579309689584 1102 30-31 0.4010508936430881 1102 32-33 0.5173400253549758 1102 34-35 0.42377549569825845 1102 36-37 0.3773189207428729 1102 38-39 0.4064998358923688 1102 40-41 0.4735551605083259 1102 42-43 0.6307072021563229 1102 44-45 0.5358559921159767 1102 46-47 0.47270377350118764 1102 48-49 0.5777737214354843 1102 50-51 0.4459611724731616 1102 52-53 0.426480659414203 1102 54-55 0.48933513256563543 1102 56-57 0.4609405006987899 1102 58-59 0.5497783024041425 1102 60-61 0.42327285316619623 1102 62-63 0.4106556171956157 1102 64-65 0.29356982829264666 1102 66-67 0.23165521420467883 1102 68-69 0.269052616604597 1102 70-71 0.2999454177233112 1102 72-73 0.217565224739257 1102 74-75 0.2766151751686792 1102 76-77 0.30100487259916164 1102 78-79 0.236787604350841 1102 80-81 0.3053552779035833 1102 82-83 0.36029556103936855 1102 84-85 0.35898359036895044 1102 86-87 0.4094290979306301 1102 88-89 0.389235581812418 1102 90-91 0.38078484055266415 1102 92-93 0.444737346331074 1102 94-95 0.5216807526636842 1102 96-97 0.6179340636970352 1102 98-99 0.4298677336503438 1102 100-101 0.3643344654659657 1102 102-103 0.33323797645297404 1102 104-105 0.46644900145386714 1102 106-107 0.42397791645959515 1102 108-109 0.3644688511413072 1102 110-111 0.3066584890236115 1102 112-113 0.35031208576218376 1102 114-115 0.3301810552144069 1102 116-117 0.35624227653685026 1102 118-119 0.3832677501353565 1102 120-121 0.40488322089866813 1102 122-123 0.34241697559322404 1102 124-125 0.36075848773354835 1103 1 -0.1252345545807323 1103 2 -0.07396135461570452 1103 3 0.0935898852497381 1103 4 -0.002631540914634911 1103 5 0.06890624553805225 1103 6 0.002824826376695455 1103 7 0.1311941026515555 1103 8 0.07433375837964462 1103 9 0.08076882660408558 1103 10-11 0.18628234366436658 1103 12-13 0.014329502031827701 1103 14-15 0.21202377766802272 1103 16-17 0.12961168842699777 1103 18-19 0.08048281512868982 1103 20-21 0.0015596520726006702 1103 22-23 0.11848802709437223 1103 24-25 0.11004636345787588 1103 26-27 -0.09602488925585106 1103 28-29 0.15366380335743912 1103 30-31 0.05251616749967525 1103 32-33 0.17682054483548626 1103 34-35 0.06765936545168927 1103 36-37 0.052823343855990856 1103 38-39 0.2362767980606364 1103 40-41 0.20622408578594786 1103 42-43 0.3506112111907669 1103 44-45 0.29416485716397744 1103 46-47 0.16174104058192995 1103 48-49 -3.04577077599788E-4 1103 50-51 0.0864232466308934 1103 52-53 0.22355952258002532 1103 54-55 0.0831625370452116 1103 56-57 0.08403762096981637 1103 58-59 -0.018348932679614904 1103 60-61 -0.02657374950648972 1103 62-63 -0.10310120569164383 1103 64-65 -0.10278722045570277 1103 66-67 -0.09429528457190628 1103 68-69 -0.18272227517029194 1103 70-71 -0.12836062406099202 1103 72-73 -0.17107302107290323 1103 74-75 -0.2797014068000756 1103 76-77 -0.2737080955920703 1103 78-79 -0.32555005798682046 1103 80-81 -0.2161777542816239 1103 82-83 -0.2912958185954899 1103 84-85 -0.09232922724821435 1103 86-87 -0.18361061597865103 1103 88-89 -0.24835523316970054 1103 90-91 -0.2822454624776398 1103 92-93 -0.3885809295958964 1103 94-95 -0.18542257878747392 1103 96-97 -0.11367613769577645 1103 98-99 -0.24499769078029843 1103 100-101 -0.19008488330120699 1103 102-103 -0.28933966329857697 1103 104-105 -0.3485613505130054 1103 106-107 -0.29313106151894885 1103 108-109 -0.20880895800607036 1103 110-111 -0.26610642721956523 1103 112-113 -0.2534993202231348 1103 114-115 -0.18590590130733275 1103 116-117 -0.2169574223150228 1103 118-119 -0.35879040909671644 1103 120-121 -0.3809411720996394 1103 122-123 -0.35643301499589697 1103 124-125 -0.44165540272382486 1104 1 0.5426206910393212 1104 2 0.6295058328024616 1104 3 0.6812096383203254 1104 4 0.722130930346232 1104 5 0.8100824897782033 1104 6 0.5363383170684699 1104 7 0.4497380720563342 1104 8 0.42117408369968956 1104 9 0.6149621160497887 1104 10-11 0.8181556862540802 1104 12-13 0.6577387141600397 1104 14-15 0.7803042716123656 1104 16-17 0.7683299150500957 1104 18-19 0.5439507133427242 1104 20-21 0.5972190141092568 1104 22-23 0.6516598815019989 1104 24-25 0.696066959436159 1104 26-27 0.5330164929588648 1104 28-29 0.6312769401476928 1104 30-31 0.7290670385719906 1104 32-33 0.7908038769396271 1104 34-35 0.7039439178800961 1104 36-37 0.7346015039314935 1104 38-39 0.956175261138263 1104 40-41 0.7669541105515734 1104 42-43 0.8783474813402279 1104 44-45 0.822760094724778 1104 46-47 0.7942717357402955 1104 48-49 0.7157509120301242 1104 50-51 0.7047171815647388 1104 52-53 0.6606407275340089 1104 54-55 0.7138940635675226 1104 56-57 0.8009643694474846 1104 58-59 0.7996151769389996 1104 60-61 0.7811721350923264 1104 62-63 0.7600222237434267 1104 64-65 0.8034052277924744 1104 66-67 0.7217845479581726 1104 68-69 0.7311848467905264 1104 70-71 0.8191321370442601 1104 72-73 0.826261360817945 1104 74-75 0.8058102753704901 1104 76-77 0.6873058199739326 1104 78-79 0.7216879389156041 1104 80-81 0.7020495903025932 1104 82-83 0.8031752317042695 1104 84-85 0.9241627065547782 1104 86-87 0.8456314190993943 1104 88-89 0.8156014307286021 1104 90-91 0.7693684716866294 1104 92-93 0.7434208105178968 1104 94-95 0.6774087830225852 1104 96-97 0.6119490420073177 1104 98-99 0.66841187407368 1104 100-101 0.701257676483138 1104 102-103 0.6139858192142391 1104 104-105 0.6341088583754697 1104 106-107 0.7911174882971537 1104 108-109 0.8121513699647629 1104 110-111 0.8707869779843165 1104 112-113 0.80913916472349 1104 114-115 0.7457382496977765 1104 116-117 0.8658254450461946 1104 118-119 0.8183384512463263 1104 120-121 0.7932191802413371 1104 122-123 0.6640750490633778 1104 124-125 0.6276908841961415 1105 1 0.3558896249708283 1105 2 0.4327123601986216 1105 3 0.3605825287456206 1105 4 0.47865782959767955 1105 5 0.23411923175011395 1105 6 0.3345833392175379 1105 7 0.42783301723681433 1105 8 0.25233252495380754 1105 9 0.4815551355772598 1105 10-11 0.3325259012169042 1105 12-13 0.3326084324754177 1105 14-15 0.3176358652412574 1105 16-17 0.48622593449545803 1105 18-19 0.40054030158303533 1105 20-21 0.2809691494515718 1105 22-23 0.3140465927963163 1105 24-25 0.5522570590219473 1105 26-27 0.4228233993657966 1105 28-29 0.536818730453156 1105 30-31 0.5084046746138924 1105 32-33 0.7571143644910094 1105 34-35 0.5962098698347589 1105 36-37 0.5865498316759101 1105 38-39 0.5807164703217751 1105 40-41 0.4677874530384898 1105 42-43 0.5311539185604914 1105 44-45 0.574543474846017 1105 46-47 0.5484585860645126 1105 48-49 0.5780620147025033 1105 50-51 0.5133923228830355 1105 52-53 0.6497301758552183 1105 54-55 0.6144221731845931 1105 56-57 0.7161408322821003 1105 58-59 0.8978772247583819 1105 60-61 0.8144960271553288 1105 62-63 0.7928505144968909 1105 64-65 0.8797741204543854 1105 66-67 0.8260551405195002 1105 68-69 0.79549454347206 1105 70-71 0.6611860919427137 1105 72-73 0.730258418070747 1105 74-75 0.6833398689146506 1105 76-77 0.5776420651937961 1105 78-79 0.615939303715308 1105 80-81 0.4921776045131452 1105 82-83 0.5299400300455339 1105 84-85 0.4407695349208538 1105 86-87 0.46349744093513934 1105 88-89 0.4240490662003715 1105 90-91 0.5503279924362374 1105 92-93 0.47979491287800613 1105 94-95 0.5949505325292073 1105 96-97 0.5656056120068804 1105 98-99 0.5565749271660501 1105 100-101 0.5614128480762623 1105 102-103 0.5438560109007966 1105 104-105 0.5000356275936859 1105 106-107 0.5229232973198705 1105 108-109 0.48173881521978146 1105 110-111 0.5462366413677202 1105 112-113 0.5494278636013661 1105 114-115 0.5375974685882632 1105 116-117 0.5419298513733573 1105 118-119 0.5607781121138053 1105 120-121 0.5938451809244576 1105 122-123 0.5798803632693748 1105 124-125 0.5951326242353403 1106 1 -0.24941762225515518 1106 2 -0.38350387251338347 1106 3 -0.04414428122984404 1106 4 -0.10384696160360107 1106 5 -0.18515970771595391 1106 6 -0.19898863866359306 1106 7 -0.44499263186414595 1106 8 -0.4181579475746062 1106 9 -0.2727508421329716 1106 10-11 -0.0861755001331801 1106 12-13 -0.2477670207050835 1106 14-15 -0.3825486112665928 1106 16-17 -0.30845735794482465 1106 18-19 -0.30463373804050065 1106 20-21 -0.251449278057855 1106 22-23 -0.4377134870366888 1106 24-25 -4.171096842907964 1106 26-27 -1.1841584795175208 1106 28-29 -0.7747298956622402 1106 30-31 -0.605240636112292 1106 32-33 -5.431649047820848 1106 34-35 -1.1322411683605793 1106 36-37 -0.3574419408854581 1106 38-39 -0.08886382654961267 1106 40-41 -0.6418639184047308 1106 42-43 -0.299638857406606 1106 44-45 -0.22432525234237488 1106 46-47 -0.39144998438751344 1106 48-49 -0.16697436180657377 1106 50-51 -0.13715897043613978 1106 52-53 -0.03722585495495423 1106 54-55 -0.2553296303822634 1106 56-57 -0.02703399468534684 1106 58-59 0.02470215365329409 1106 60-61 0.052615679015048045 1106 62-63 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36-37 -0.9551393853395354 2216 38-39 -0.8183140013495205 2216 40-41 -1.0145362624571987 2216 42-43 -0.6486473012539307 2216 44-45 -0.6557379535067369 2216 46-47 -0.7807147773723955 2216 48-49 -0.9946077370341015 2216 50-51 -1.066407439131062 2216 52-53 -1.1491582969531677 2216 54-55 -1.1119566781416381 2216 56-57 -1.0217954144651884 2216 58-59 -1.0039809239809827 2216 60-61 -1.3305482717520505 2216 62-63 -1.329219534630976 2216 64-65 -1.159525215153117 2216 66-67 -1.0295005365194143 2216 68-69 -1.0121266088462058 2216 70-71 -1.0666523819590203 2216 72-73 -0.9898395916673621 2216 74-75 -1.1477994749294247 2216 76-77 -0.8956672053229191 2216 78-79 -0.8165572522314228 2216 80-81 -0.914868491133138 2216 82-83 -0.847887191459936 2216 84-85 -1.0545231367169094 2216 86-87 -0.9648685289029721 2216 88-89 -0.9111119842905424 2216 90-91 -0.9005865039301924 2216 92-93 -0.9694970972828614 2216 94-95 -0.9782850279585702 2216 96-97 -1.0650246861861774 2216 98-99 -1.0606358634719548 2216 100-101 -1.0525024027018368 2216 102-103 -1.0445998961061207 2216 104-105 -1.0939569594916634 2216 106-107 -1.1452608483726934 2216 108-109 -1.2049199884363837 2216 110-111 -1.1594555677828922 2216 112-113 -1.3074491727634623 2216 114-115 -1.2522585645183817 2216 116-117 -1.1339699303210402 2216 118-119 -1.133449408605184 2216 120-121 -1.1596469341980367 2216 122-123 -0.9634525236126059 2216 124-125 -1.1555459362597276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42748.0 3 6304.0 4 5389.0 5 4756.0 6 5642.0 7 5224.0 8 4935.0 9 5382.0 10 6522.0 11 8018.0 12 8500.0 13 9065.0 14 9033.0 15 8617.0 16 7815.0 17 6848.0 18 5856.0 19 5327.0 20 4692.0 21 4735.0 22 5626.0 23 8202.0 24 13470.0 25 20807.0 26 23715.0 27 19127.0 28 21076.0 29 26245.0 30 30034.0 31 36152.0 32 47148.0 33 62083.0 34 86572.0 35 121621.0 36 202228.0 37 296129.0 38 102392.0 39 374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.99029184990399 16.773413887901448 8.232911777535271 21.00338248465929 2 19.092414120693228 21.356760713035555 38.25406102264269 21.296764143628526 3 17.634462348524423 32.24729103879281 28.29086105421493 21.827385558467846 4 10.198214532420783 16.588456087463385 35.58780566934854 37.62552371076729 5 9.983824698226616 44.92922917391088 32.443588246982266 12.643357880880234 6 28.64386625871819 37.22638501456848 20.833313929391384 13.296434797321947 7 30.100280194661554 35.65969931464851 19.395447030790365 14.844573459899566 8 28.954370321615187 34.75277326734825 18.72300857205838 17.569847838978184 9 30.06098446010086 15.677903941519498 16.793895499786164 37.46721609859349 10-11 24.209998924997002 29.501885524211435 27.361426486288664 18.926689064502895 12-13 24.646701734532066 28.064072203477448 26.816205731477016 20.473020330513467 14-15 22.64234713163457 29.044581933283503 24.656711274214167 23.65635966086776 16-17 22.31899120502286 30.942508590451812 25.276652521948634 21.461847682576693 18-19 21.676377411564175 29.520289331427534 27.182530827232824 21.62080242977546 20-21 23.905640145163037 28.726788525895053 26.40609712697186 20.96147420197005 22-23 23.018499700114052 29.25092218814192 25.925528840327043 21.805049271416983 24-25 22.394310880394674 28.80885222539768 26.61671403977478 22.180122854432867 26-27 22.224662867539195 28.71846975160785 26.660941067988443 22.39592631286451 28-29 22.22095446997154 29.111407868627747 26.43077160705838 22.23686605434233 30-31 22.833549560863425 28.553754112236252 26.875018676547807 21.737677650352516 32-33 22.154505068631533 28.76493510575997 27.077021233493138 22.00353859211536 34-35 22.71906301299689 28.84165059357257 26.84240347411682 21.596882919313714 36-37 22.601692968267322 28.68394458812097 26.631534910291 22.082827533320707 38-39 22.30409506512054 28.976854654682626 26.779715611853643 21.939334668343193 40-41 22.444035056604317 28.85968238247682 26.912420971092622 21.78386158982624 42-43 22.116645891190316 28.78068392971628 26.807738101706175 22.294932077387227 44-45 21.88440257952547 29.099413098577937 26.987569239271288 22.028615082625304 46-47 22.136286530269143 28.699830366592117 26.579703834273865 22.584179268864876 48-49 22.03849736106799 28.75721825520025 26.802118907171685 22.402165476560075 50-51 22.003768320109284 28.86218853631228 27.141141246029388 21.99290189754904 52-53 22.102446064831256 29.11173799874338 26.75241030274171 22.03340563368366 54-55 21.851384999410108 29.177545995889396 26.947024781585498 22.02404422311499 56-57 22.032453183418856 29.317033264280152 26.55332786392824 22.097185688372754 58-59 22.11820379410483 28.934715985450097 26.778697657198673 22.1683825632464 60-61 22.461656438741695 29.04893686628487 26.925347208073365 21.564059486900067 62-63 22.009883745298353 29.145988211534192 26.985170501203836 21.858957541963616 64-65 21.640450999256046 28.969285930780703 27.134777804900484 22.25548526506277 66-67 21.809404872415 29.34780921393733 26.48281273769866 22.359973175949012 68-69 21.92141520772397 29.08155008772478 26.775442029269534 22.221592675281716 70-71 21.70228982749066 28.85351841129344 27.135953753277338 22.308238007938566 72-73 21.819303712730083 29.24422056729056 26.72249797229908 22.21397774768027 74-75 21.774251447043916 29.587687077601917 26.732601031121874 21.905460444232293 76-77 21.51608019705038 29.28869610909332 26.943619671352447 22.251604022503855 78-79 21.84771092940496 28.98843919419748 27.02073493971228 22.143114936685283 80-81 21.73096222521129 29.270629672950694 26.48783490207614 22.510573199761875 82-83 21.889506793283168 29.68689969380746 26.41048428095203 22.013109231957344 84-85 21.72416750523808 29.486150085978636 26.517975957028924 22.271706451754365 86-87 21.469843468947012 29.414100019209837 26.736093728473826 22.37996278336933 88-89 21.673609380930316 29.113529095367046 27.007973449259975 22.20488807444266 90-91 21.954962090680443 29.152163898964883 26.860374098642236 22.032499911712442 92-93 21.451897639210078 29.429018160847903 26.75171248758638 22.36737171235564 94-95 21.432604665548745 29.072614398539585 26.980564676461494 22.51421625945018 96-97 21.586294923503868 29.299708126018466 26.836883153775794 22.277113796701876 98-99 21.30127592659716 29.367345624058423 26.769427435798125 22.56195101354629 100-101 21.151401996826284 29.47753968213928 26.880547844623674 22.49051047641076 102-103 21.079503162476552 29.46637234561922 26.97081393895788 22.483310552946353 104-105 21.023149750774998 29.552178914214128 26.89945777456275 22.525213560448123 106-107 20.9547619149259 29.8194380680762 27.012029312450498 22.213770704547404 108-109 21.123125188218527 29.89073235247548 26.551619222661355 22.434523236644637 110-111 20.99532205773576 30.311661014896924 26.5129576749843 22.180059252383018 112-113 20.9080579137813 30.25831303681005 26.52091977789472 22.312709271513924 114-115 20.688140329090345 30.552778640173862 26.27274138466315 22.486339646072647 116-117 20.763255507459316 30.28999781898169 26.60857862027695 22.338168053282047 118-119 20.763500858829804 30.064204610282207 26.625120985174416 22.547173545713576 120-121 20.81275698205322 30.13390717502624 26.551497311572366 22.501838531348174 122-123 20.828504967040338 30.037923211318287 26.440767685015366 22.692804136626012 124-125 20.94514828845988 29.875908199388235 26.291814427340555 22.887129084811328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1374.0 1 1601.5 2 1872.0 3 1783.5 4 1451.5 5 1128.5 6 924.0 7 835.0 8 795.5 9 761.0 10 725.0 11 653.0 12 662.5 13 709.0 14 713.5 15 754.5 16 792.0 17 877.5 18 1038.0 19 1260.0 20 1548.5 21 2124.0 22 2921.5 23 3734.5 24 4712.0 25 6067.0 26 8055.5 27 10454.0 28 12362.0 29 13668.0 30 16255.0 31 19463.5 32 22489.0 33 26178.5 34 30076.5 35 32562.0 36 35142.0 37 38282.5 38 39443.0 39 40229.5 40 40002.0 41 40026.5 42 40532.5 43 41501.0 44 44194.0 45 46031.5 46 47145.5 47 46968.5 48 47116.0 49 47840.5 50 48365.5 51 47363.5 52 44172.5 53 41860.5 54 38883.5 55 35214.5 56 32312.5 57 29931.5 58 27438.5 59 23783.5 60 19979.0 61 16726.5 62 13365.5 63 9969.0 64 7405.0 65 5772.5 66 4357.5 67 3372.5 68 2691.5 69 1825.5 70 1254.5 71 1002.0 72 938.0 73 933.0 74 731.0 75 517.0 76 337.0 77 222.5 78 164.5 79 108.5 80 83.0 81 57.0 82 31.5 83 21.0 84 15.0 85 9.5 86 6.5 87 8.0 88 8.5 89 5.5 90 3.5 91 2.0 92 2.0 93 2.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.433057359891153E-4 2 1.5523021028260436E-4 3 0.0 4 5.433057359891153E-4 5 0.0019403776285325544 6 8.537661565543239E-4 7 0.0014746869976847415 8 0.002871758890228181 9 0.0037255250467825044 10-11 0.0031434117582227385 12-13 0.003880755257065109 14-15 0.004812136518760735 16-17 0.004656906308478131 18-19 0.004618098755907479 20-21 0.00500617428161399 22-23 0.09725172674205163 24-25 0.00426883078277162 26-27 0.0033374495210759933 28-29 0.003065796653081436 30-31 0.002212030496527112 32-33 0.003376257073646645 34-35 0.0018627625233912522 36-37 0.0016299172079673459 38-39 0.002250838049097763 40-41 0.0027553362325162273 42-43 0.0024060682593803674 44-45 0.002522490917092321 46-47 0.0032986419685053425 48-49 6.985359462717196E-4 50-51 0.0028329513376575298 52-53 0.002600106022233623 54-55 0.0025612984696629718 56-57 0.0023284531542390654 58-59 0.0012030341296901837 60-61 0.0019015700759619034 62-63 0.0024060682593803674 64-65 0.001979185181103206 66-67 0.0010089963668369283 68-69 3.492679731358598E-4 70-71 0.0011642265771195327 72-73 3.104604205652087E-4 74-75 5.044981834184642E-4 76-77 5.821132885597663E-4 78-79 3.104604205652087E-4 80-81 7.761510514130217E-4 82-83 3.104604205652087E-4 84-85 5.821132885597663E-4 86-87 5.044981834184642E-4 88-89 6.209208411304174E-4 90-91 4.2688307827716196E-4 92-93 4.2688307827716196E-4 94-95 5.821132885597663E-4 96-97 8.149586039836729E-4 98-99 9.313812616956261E-4 100-101 0.0011254190245488815 102-103 0.0015523021028260434 104-105 0.0011642265771195327 106-107 0.0011254190245488815 108-109 0.0010089963668369283 110-111 0.0017075323131086478 112-113 8.537661565543239E-4 114-115 6.985359462717196E-4 116-117 0.0015523021028260434 118-119 0.002173222943956461 120-121 0.0012806492348314859 122-123 0.0012418416822608349 124-125 6.209208411304174E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1288409.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.44930782893893 #Duplication Level Percentage of deduplicated Percentage of total 1 56.687920785529 19.52859633323194 2 12.169658184426597 8.384726019365555 3 8.695547927246745 8.986668218610447 4 6.403559184246962 8.823927261558104 5 4.594505913153727 7.913877426205643 6 3.18094258802004 6.574876224053041 7 2.2118938944489583 5.333874955837588 8 1.4503883999328055 3.997190116864592 9 0.9839830336241248 3.050778098139357 >10 3.392319922192414 19.080769003852303 >50 0.15884863253601747 3.7284806569821813 >100 0.06885134242477534 4.093553231147965 >500 0.0011287087270310928 0.26681675436156227 >1k 4.514834908124372E-4 0.23586569978974592 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2005 0.15561828580831086 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 7.761510514130218E-5 22-23 0.0 0.0 0.0 2.3284531542390653E-4 1.5523021028260436E-4 24-25 0.0 7.761510514130218E-5 0.0 6.597283937010685E-4 1.5523021028260436E-4 26-27 0.0 7.761510514130218E-5 0.0 0.0012418416822608349 1.5523021028260436E-4 28-29 0.0 7.761510514130218E-5 0.0 0.001746339865679299 1.5523021028260436E-4 30-31 0.0 7.761510514130218E-5 0.0 0.0038419477044944576 1.5523021028260436E-4 32-33 0.0 7.761510514130218E-5 0.0 0.009701888142662773 1.5523021028260436E-4 34-35 0.0 7.761510514130218E-5 0.0 0.01893808565447773 1.5523021028260436E-4 36-37 0.0 7.761510514130218E-5 0.0 0.031744578002792595 1.5523021028260436E-4 38-39 0.0 7.761510514130218E-5 0.0 0.056232143674873426 1.5523021028260436E-4 40-41 0.0 7.761510514130218E-5 0.0 0.08584230628628021 1.5523021028260436E-4 42-43 0.0 7.761510514130218E-5 0.0 0.11502558581940983 1.5523021028260436E-4 44-45 0.0 7.761510514130218E-5 0.0 0.15445405923119132 1.5523021028260436E-4 46-47 0.0 7.761510514130218E-5 0.0 0.20117835252625527 1.5523021028260436E-4 48-49 0.0 7.761510514130218E-5 0.0 0.2506579820538354 1.5523021028260436E-4 50-51 0.0 7.761510514130218E-5 0.0 0.3023496420779427 1.5523021028260436E-4 52-53 0.0 7.761510514130218E-5 0.0 0.3591638990413758 1.5523021028260436E-4 54-55 0.0 7.761510514130218E-5 0.0 0.42936676164168364 1.5523021028260436E-4 56-57 0.0 7.761510514130218E-5 0.0 0.5283648282494147 1.5523021028260436E-4 58-59 0.0 7.761510514130218E-5 0.0 0.6383454322346398 1.5523021028260436E-4 60-61 2.3284531542390653E-4 7.761510514130218E-5 0.0 0.7399047973120336 1.5523021028260436E-4 62-63 2.3284531542390653E-4 7.761510514130218E-5 0.0 0.8457329931721992 1.5523021028260436E-4 64-65 2.3284531542390653E-4 7.761510514130218E-5 0.0 0.9658035608257937 1.5523021028260436E-4 66-67 2.3284531542390653E-4 7.761510514130218E-5 0.0 1.125496639654023 1.5523021028260436E-4 68-69 2.3284531542390653E-4 7.761510514130218E-5 0.0 1.3414218621571257 1.5523021028260436E-4 70-71 2.3284531542390653E-4 7.761510514130218E-5 0.0 1.5423285618153864 1.5523021028260436E-4 72-73 2.3284531542390653E-4 7.761510514130218E-5 0.0 1.721308994271229 1.5523021028260436E-4 74-75 2.3284531542390653E-4 7.761510514130218E-5 0.0 1.9153467571244844 1.5523021028260436E-4 76-77 2.3284531542390653E-4 7.761510514130218E-5 0.0 2.1552938546688205 1.5523021028260436E-4 78-79 2.3284531542390653E-4 7.761510514130218E-5 0.0 2.5087530434823107 1.5523021028260436E-4 80-81 2.3284531542390653E-4 7.761510514130218E-5 0.0 2.817428316629269 1.5523021028260436E-4 82-83 2.3284531542390653E-4 1.1642265771195326E-4 0.0 3.112055255745652 1.5523021028260436E-4 84-85 2.3284531542390653E-4 1.5523021028260436E-4 0.0 3.41828565308066 1.5523021028260436E-4 86-87 2.3284531542390653E-4 1.5523021028260436E-4 0.0 3.77768239743746 1.5523021028260436E-4 88-89 2.3284531542390653E-4 1.5523021028260436E-4 0.0 4.242286416813295 1.5523021028260436E-4 90-91 2.3284531542390653E-4 1.5523021028260436E-4 0.0 4.713060837047863 1.5523021028260436E-4 92-93 2.3284531542390653E-4 1.5523021028260436E-4 0.0 5.140099145535308 1.5523021028260436E-4 94-95 2.3284531542390653E-4 1.5523021028260436E-4 0.0 5.606992810512811 1.5523021028260436E-4 96-97 2.3284531542390653E-4 1.5523021028260436E-4 0.0 6.089370688966004 1.5523021028260436E-4 98-99 2.3284531542390653E-4 1.5523021028260436E-4 0.0 6.652817544739287 1.5523021028260436E-4 100-101 2.3284531542390653E-4 1.5523021028260436E-4 0.0 7.271603970478319 1.5523021028260436E-4 102-103 2.3284531542390653E-4 1.5523021028260436E-4 0.0 7.894659227000122 1.5523021028260436E-4 104-105 2.3284531542390653E-4 1.5523021028260436E-4 0.0 8.51387253581743 1.5523021028260436E-4 106-107 2.3284531542390653E-4 1.5523021028260436E-4 0.0 9.172320280283667 1.5523021028260436E-4 108-109 2.3284531542390653E-4 1.5523021028260436E-4 0.0 9.896508018804589 1.5523021028260436E-4 110-111 2.3284531542390653E-4 1.5523021028260436E-4 0.0 10.66256910654924 1.5523021028260436E-4 112-113 2.3284531542390653E-4 1.5523021028260436E-4 0.0 11.356486954065051 1.5523021028260436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2265 0.0 75.9107 2 GTACATG 14655 0.0 54.520966 1 TACATGG 14870 0.0 52.93248 2 ACCTGGG 3260 0.0 50.91666 3 GTACCTG 3435 0.0 50.40105 1 ACATGGG 15425 0.0 49.09944 3 CATGGGG 3850 0.0 46.668037 4 CATGGGA 11330 0.0 44.686153 4 ATGGGAT 4175 0.0 42.7502 5 TGGGATA 1410 0.0 42.616283 6 ATGGGAG 3425 0.0 41.86295 5 ATGGGGG 2525 0.0 39.81976 5 GGGATAG 775 0.0 38.38324 7 GGTATCA 3190 0.0 37.486828 1 TATCACG 80 1.19825905E-4 37.183765 2 GAACGCA 395 0.0 34.64209 9 CCTGGGA 3195 0.0 33.14534 4 ATGGGAC 2095 0.0 32.9418 5 GGAACGC 380 0.0 32.878277 8 GTCGGGA 510 0.0 32.663383 2 >>END_MODULE