FastQCFastQC Report
Thu 26 May 2016
SRR936252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288409
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96870.7518575235037942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70950.550679170977539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68300.5301111681150938No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.1991603597925814No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19250.14940907739700668No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.10415947109962752No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.10035633094770371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20050.073.0486152
GGTATCA58700.068.5110551
TAACGCA3350.065.757944
TATCACG1500.063.5067672
TATAACG3450.062.1261862
ATAACGC3850.060.310813
ACCTGGG28850.051.179633
GTACATG123250.047.0349731
ATCACGC2050.046.4683653
TACATGG126150.045.8271942
ACATGGG124850.045.7798043
GTATCAA89900.044.769092
CACGCAG3350.042.6537975
CATGGGA91150.042.064964
CATGGGG32650.041.7583734
ATGGGAG28600.041.010165
GTACCTG36100.040.5997051
GTATAAC6750.039.7194941
ATGGGAT35250.036.989315
TACAACG2400.034.730262
Produced by FastQC (version 0.11.5)