FastQCFastQC Report
Thu 26 May 2016
SRR936251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419136
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50900.058.909191
GTACATG273200.055.3342821
TACATGG274650.054.2839932
ACATGGG282450.051.3736653
CATGGGA196150.047.558494
CATGGGG84450.042.9030274
GTATCAA71600.041.795092
ATGGGAG74050.041.697755
ATGGGAT80000.038.968315
TCAACGC81150.037.3896985
ATCAACG81650.037.087874
CAACGCA84450.036.1399886
AACGCAG86850.034.7302937
GAGTACA153900.031.7763141
TACCTGG31100.031.755432
TATCAAC96200.031.5402723
AGTACAT149750.031.5047972
ATGGGGG46350.030.9341095
ATGGGAC41150.029.060065
ATGGGGA50850.029.0155665
Produced by FastQC (version 0.11.5)