FastQCFastQC Report
Thu 26 May 2016
SRR936251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419136
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116770.34151902702905057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92680.27106263102725364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92230.2697465090595987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85650.077.135221
GTATCAA132500.049.9604072
GTACATG232000.048.839481
TACATGG235650.047.745382
ACATGGG237450.046.8569683
CATGGGA163300.042.3965534
CATGGGG73550.039.5788544
ATCAACG177650.038.2681924
TCAACGC178450.038.0632745
CAACGCA181250.037.6825646
AACGCAG186450.036.791167
ATGGGAT71350.036.5440945
TATCAAC190800.035.755533
ATGGGAG62350.034.5628175
ACGCAGA215250.031.6751028
CGCAGAG218400.031.000329
GAGTACA145450.029.4485361
TACCTGG30750.029.03912
AGTACAT142150.028.728592
ATGGGGG42450.027.7667185
Produced by FastQC (version 0.11.5)