FastQCFastQC Report
Thu 26 May 2016
SRR936250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306646
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44240.33857678361239385No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14380.11005276103856745No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14340.10974663374777867No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13310.1018638560099675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG155400.056.775051
TACATGG156400.055.1567462
GGTATCA30700.053.291961
ACATGGG158650.052.0495263
CATGGGG38300.048.3091054
CATGGGA118250.048.1983034
ATGGGAG41800.046.256685
ATGGGAT42500.041.2952845
ATGGGGG23250.040.9415635
GTATCAA42900.037.997992
ATGGGCG2051.8189894E-1234.8252945
ATGGGAC21300.033.796615
TACCTGG11450.033.2538452
GAGTACA88550.033.12271
CGGGAGT3800.032.8778274
AGTACAT86100.032.6141662
GAACAAA27100.032.271221
CATGGGT10200.032.0796744
ATCAACG51850.032.0127374
TCAACGC51800.031.9287855
Produced by FastQC (version 0.11.5)