##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936250_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1306646 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.158042805779072 31.0 30.0 34.0 22.0 34.0 2 29.09017897732056 31.0 30.0 34.0 20.0 34.0 3 29.19277753882842 31.0 30.0 34.0 22.0 34.0 4 32.28680300555774 35.0 33.0 37.0 18.0 37.0 5 32.846487878124606 35.0 33.0 37.0 26.0 37.0 6 32.56772071395007 37.0 33.0 37.0 21.0 37.0 7 32.522899851987454 37.0 33.0 37.0 21.0 37.0 8 32.38357137281253 37.0 33.0 37.0 18.0 37.0 9 33.49286264221526 38.0 34.0 39.0 13.0 39.0 10-11 33.50449356596967 38.0 33.5 39.0 16.0 39.0 12-13 33.52781510830018 38.0 34.0 39.0 16.0 39.0 14-15 34.63574908582738 39.0 34.0 41.0 17.0 41.0 16-17 34.745420718388914 39.0 34.0 41.0 17.5 41.0 18-19 34.7977233313384 39.0 34.0 41.0 18.5 41.0 20-21 34.91095101504156 39.0 34.0 41.0 21.5 41.0 22-23 34.860262458232754 39.0 34.0 41.0 23.0 41.0 24-25 34.89629555365416 39.0 34.0 41.0 24.5 41.0 26-27 35.010699914131294 39.0 34.0 41.0 25.0 41.0 28-29 34.91204121085589 39.0 34.0 41.0 25.0 41.0 30-31 34.83939797006994 39.0 34.0 41.0 25.0 41.0 32-33 34.67249928442746 39.0 33.0 41.0 24.5 41.0 34-35 34.626358631182434 39.0 33.0 41.0 24.0 41.0 36-37 34.51125476984585 39.0 33.0 41.0 24.0 41.0 38-39 34.27390318418301 38.0 33.0 40.5 23.0 41.0 40-41 34.37157347896829 38.5 33.0 40.5 23.5 41.0 42-43 34.29249429455262 39.0 33.0 41.0 22.5 41.0 44-45 34.221736415218814 39.0 33.0 41.0 22.5 41.0 46-47 34.21759757424735 39.0 33.0 41.0 23.0 41.0 48-49 34.13926878435322 38.5 33.0 40.5 22.5 41.0 50-51 34.04232209795155 38.0 33.0 40.0 22.0 41.0 52-53 33.90612377032494 38.0 33.0 40.0 21.5 41.0 54-55 33.75856735489184 38.0 32.5 40.0 20.5 41.0 56-57 33.59087312095242 38.0 32.0 40.0 19.5 41.0 58-59 33.36414338696173 38.0 32.0 40.0 18.0 41.0 60-61 33.13200323576547 37.5 31.5 40.0 15.5 41.0 62-63 32.883181825835 37.0 31.0 40.0 13.0 41.0 64-65 32.623767263665904 36.5 31.0 40.0 10.5 41.0 66-67 32.3279905192378 36.0 31.0 39.5 8.0 41.0 68-69 32.02751739950989 36.0 31.0 39.0 7.0 41.0 70-71 31.692193218362128 35.0 31.0 39.0 2.0 40.5 72-73 31.353816182806973 35.0 30.5 38.5 2.0 40.0 74-75 31.00316038161828 35.0 30.0 37.5 2.0 40.0 76-77 30.662380246830438 35.0 30.0 37.0 2.0 39.0 78-79 30.30528620605734 35.0 30.0 36.5 2.0 39.0 80-81 29.970229503629902 34.5 30.0 36.0 2.0 38.5 82-83 29.652732645261224 34.0 29.5 36.0 2.0 37.0 84-85 29.632511407068172 34.0 30.0 35.0 2.0 37.0 86-87 29.481397409856992 34.0 30.0 35.0 2.0 37.0 88-89 29.284797106484845 34.0 30.0 35.0 2.0 36.0 90-91 29.091637291202055 34.0 29.0 35.0 2.0 36.0 92-93 28.94633397262916 34.0 29.0 35.0 2.0 36.0 94-95 28.81412333562419 34.0 29.0 35.0 2.0 35.5 96-97 28.69656701202927 34.0 29.0 35.0 2.0 35.0 98-99 28.57820519100047 34.0 29.0 35.0 2.0 35.0 100-101 28.4617084504908 34.0 29.0 35.0 2.0 35.0 102-103 27.53832637148853 33.0 26.5 34.5 2.0 35.0 104-105 28.14624006808271 34.0 28.5 35.0 2.0 35.0 106-107 28.231615142892565 34.0 29.0 35.0 2.0 35.0 108-109 28.16655352712211 34.0 29.0 35.0 2.0 35.0 110-111 28.04686770556065 34.0 28.0 35.0 2.0 35.0 112-113 27.984381385623955 34.0 28.0 35.0 2.0 35.0 114-115 27.853093339741598 34.0 28.0 35.0 2.0 35.0 116-117 27.716237603758017 34.0 28.0 35.0 2.0 35.0 118-119 27.507079958917718 34.0 27.0 35.0 2.0 35.0 120-121 27.2914297368989 34.0 27.0 35.0 2.0 35.0 122-123 27.002641495860395 34.0 27.0 35.0 2.0 35.0 124-125 25.14247049315576 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8574648498884372 1101 2 0.9147523223125731 1101 3 0.944557233411814 1101 4 1.0145733866889728 1101 5 0.7501467874480809 1101 6 0.8607556779987036 1101 7 0.7901000763546193 1101 8 0.9120584505696172 1101 9 1.070136974023299 1101 10-11 1.2125262936151984 1101 12-13 1.2602469485708312 1101 14-15 1.363380041743362 1101 16-17 1.328165968849035 1101 18-19 1.2286136743241514 1101 20-21 1.059579084382129 1101 22-23 1.2096841131258316 1101 24-25 1.1718174868629418 1101 26-27 1.0090862104593086 1101 28-29 1.133623552003641 1101 30-31 1.160721256939624 1101 32-33 1.2408909906642762 1101 34-35 1.140067082632143 1101 36-37 1.0628560782176137 1101 38-39 1.1787961276197976 1101 40-41 1.2251837828888767 1101 42-43 1.2949397559384224 1101 44-45 1.3431833999910765 1101 46-47 1.2613648685487675 1101 48-49 1.2251007928221185 1101 50-51 1.2818784044330442 1101 52-53 1.2561508776451902 1101 54-55 1.1847637683763637 1101 56-57 1.195102178532217 1101 58-59 1.3223586224669859 1101 60-61 1.3321072196955797 1101 62-63 1.332193451682869 1101 64-65 1.215001467667463 1101 66-67 1.240097530479538 1101 68-69 1.2758469453890697 1101 70-71 1.296068942020515 1101 72-73 1.26328324825014 1101 74-75 1.2277666945771877 1101 76-77 1.246534278100306 1101 78-79 1.29994282512774 1101 80-81 1.2036740477156371 1101 82-83 1.289315300249669 1101 84-85 1.167881238869949 1101 86-87 1.26363932402219 1101 88-89 1.2180487422295307 1101 90-91 1.2454692352558716 1101 92-93 1.2151848285845723 1101 94-95 1.3182921664143343 1101 96-97 1.3038754163751847 1101 98-99 1.2366588557184883 1101 100-101 1.2314727859459218 1101 102-103 1.288823063318489 1101 104-105 1.3234671215024356 1101 106-107 1.2711510284699479 1101 108-109 1.171291949169479 1101 110-111 1.3100869964679447 1101 112-113 1.1956328540196672 1101 114-115 1.2299803775747975 1101 116-117 1.3218558802786617 1101 118-119 1.3612398205112939 1101 120-121 1.3426728307865154 1101 122-123 1.331711837253188 1101 124-125 1.108491763476323 1102 1 0.5126002911912941 1102 2 0.4848727899221217 1102 3 0.48102229171924904 1102 4 0.591263159413252 1102 5 0.4678032385234374 1102 6 0.2453739009319449 1102 7 0.4454715219443699 1102 8 0.2920742305110693 1102 9 0.5757506414748121 1102 10-11 0.5623899502248833 1102 12-13 0.4836218913367887 1102 14-15 0.6668534018909398 1102 16-17 0.6132458497111912 1102 18-19 0.587975125791246 1102 20-21 0.559171887137893 1102 22-23 0.7064633135049689 1102 24-25 0.7059594004336205 1102 26-27 0.6586526693862922 1102 28-29 0.585543488807204 1102 30-31 0.669874150100604 1102 32-33 0.7689722662279124 1102 34-35 0.7049002156214073 1102 36-37 0.6597375151746405 1102 38-39 0.7178105114221864 1102 40-41 0.7814401367352488 1102 42-43 0.922644430955728 1102 44-45 0.8147452428179704 1102 46-47 0.6840998204002986 1102 48-49 0.7083331075353954 1102 50-51 0.5730669627535363 1102 52-53 0.5339933336423712 1102 54-55 0.7280461159984597 1102 56-57 0.7609728917898764 1102 58-59 0.6829058457268005 1102 60-61 0.7596114947275439 1102 62-63 0.7186854528147251 1102 64-65 0.7184173002726197 1102 66-67 0.6343388581605822 1102 68-69 0.7112463425119202 1102 70-71 0.6559183353457421 1102 72-73 0.6073657582240166 1102 74-75 0.4882013785339048 1102 76-77 0.6210156976145775 1102 78-79 0.6252703020406507 1102 80-81 0.5697417347035092 1102 82-83 0.4481979385523722 1102 84-85 0.4716393615504906 1102 86-87 0.4580101785230326 1102 88-89 0.44806906255803014 1102 90-91 0.4733348484249582 1102 92-93 0.5154518848060619 1102 94-95 0.5248106527379477 1102 96-97 0.641947364378531 1102 98-99 0.5835664687855093 1102 100-101 0.5967826236348692 1102 102-103 0.6432641249017124 1102 104-105 0.6382311902930127 1102 106-107 0.6688361019535378 1102 108-109 0.7015381148186108 1102 110-111 0.7152966533320857 1102 112-113 0.6489183811106791 1102 114-115 0.7749635134512118 1102 116-117 0.7583603045803464 1102 118-119 0.6494477764673832 1102 120-121 0.7293585471122377 1102 122-123 0.5702997121915203 1102 124-125 0.6182650963336513 1103 1 0.20841703486073015 1103 2 0.21417553598433514 1103 3 0.289673808899348 1103 4 0.31231528478755877 1103 5 0.1920738959216095 1103 6 0.29650288110883594 1103 7 0.3367161172520312 1103 8 0.277118056339944 1103 9 0.33986799498518394 1103 10-11 0.4849043764605696 1103 12-13 0.32907817898944813 1103 14-15 0.31646156244236323 1103 16-17 0.2635624643863679 1103 18-19 0.29796727635343956 1103 20-21 -0.016174840119084877 1103 22-23 0.18434921098400991 1103 24-25 0.21641807823889536 1103 26-27 0.2193265086587317 1103 28-29 0.21442856077072747 1103 30-31 0.1376239150828482 1103 32-33 0.355592327901725 1103 34-35 0.29805316223925615 1103 36-37 0.20638439237998085 1103 38-39 0.3412903540706722 1103 40-41 0.4454614068263112 1103 42-43 0.5020284040218215 1103 44-45 0.6331059346281407 1103 46-47 0.31029057104664304 1103 48-49 0.31648291938993367 1103 50-51 0.2894490425514604 1103 52-53 0.24251344612632408 1103 54-55 0.39852795787894735 1103 56-57 0.3562279316945691 1103 58-59 0.2641701994703354 1103 60-61 0.289765722219002 1103 62-63 0.2625551946281277 1103 64-65 0.22005460851448078 1103 66-67 0.29167259339276796 1103 68-69 0.22607018122351974 1103 70-71 0.26092765610565394 1103 72-73 0.2552220550411164 1103 74-75 0.33169602270664456 1103 76-77 0.29430176248783013 1103 78-79 0.21805750638306876 1103 80-81 0.26588054636687986 1103 82-83 0.09271267551983797 1103 84-85 0.22122726009919447 1103 86-87 0.19446488650318372 1103 88-89 0.20677891950924376 1103 90-91 0.24735850747535437 1103 92-93 0.2304592986943419 1103 94-95 0.35361140708586447 1103 96-97 0.4264175297131274 1103 98-99 0.2867800367380333 1103 100-101 0.43768533046182867 1103 102-103 0.3214460051344261 1103 104-105 0.40884753941424634 1103 106-107 0.4526207242628857 1103 108-109 0.331571963580064 1103 110-111 0.2369074276580072 1103 112-113 0.23678697832378148 1103 114-115 0.15834566413034779 1103 116-117 0.23838433259489378 1103 118-119 0.1837159780821871 1103 120-121 0.1702431398519444 1103 122-123 0.22378354757623242 1103 124-125 0.3022436038140057 1104 1 0.41920101083732675 1104 2 0.4239066220040826 1104 3 0.3525163390506165 1104 4 0.4131178352645506 1104 5 0.4393358078606795 1104 6 0.27122060324924036 1104 7 0.20661494794847357 1104 8 0.22208571603902527 1104 9 0.43607070106309465 1104 10-11 0.39099154455262664 1104 12-13 0.3610822297808056 1104 14-15 0.4509971425151136 1104 16-17 0.46061365479079086 1104 18-19 0.27556698869270235 1104 20-21 0.33372338769558496 1104 22-23 0.43349225760400145 1104 24-25 0.42626938175541795 1104 26-27 0.26776482954687 1104 28-29 0.3932967650730035 1104 30-31 0.2890635106353088 1104 32-33 0.4038339944966154 1104 34-35 0.5424675343665939 1104 36-37 0.46694020631344557 1104 38-39 0.5808134889410752 1104 40-41 0.5218289436523449 1104 42-43 0.610291257604807 1104 44-45 0.5986756435510685 1104 46-47 0.5471664089805373 1104 48-49 0.45745605670055767 1104 50-51 0.4895228955688893 1104 52-53 0.5026526256903452 1104 54-55 0.3864003450323992 1104 56-57 0.3563718338325046 1104 58-59 0.375039799013301 1104 60-61 0.47987515961547444 1104 62-63 0.4779888962935175 1104 64-65 0.32256388858752416 1104 66-67 0.4702293087780234 1104 68-69 0.41211088435591847 1104 70-71 0.4665182362214644 1104 72-73 0.49737346736344534 1104 74-75 0.39191709371176486 1104 76-77 0.2498514327689172 1104 78-79 0.22304168791364276 1104 80-81 0.2715612716623639 1104 82-83 0.28833884001469556 1104 84-85 0.3220183271138062 1104 86-87 0.3287044085614781 1104 88-89 0.3231290014724557 1104 90-91 0.4612961293077156 1104 92-93 0.39891559120109576 1104 94-95 0.46130762357209676 1104 96-97 0.4485343193963871 1104 98-99 0.3860696410466282 1104 100-101 0.30303491576529495 1104 102-103 0.4180517089701894 1104 104-105 0.29213536952790164 1104 106-107 0.3778479532747028 1104 108-109 0.4214307744101191 1104 110-111 0.37934650071292353 1104 112-113 0.29462846060910763 1104 114-115 0.3095577435844241 1104 116-117 0.23628537098698033 1104 118-119 0.3227304673408433 1104 120-121 0.34724133407749136 1104 122-123 0.32573910944769224 1104 124-125 0.21119446033675615 1105 1 0.019795843550593162 1105 2 0.15707241532387073 1105 3 0.06529356336474379 1105 4 0.21269129302401524 1105 5 -0.004119906604607593 1105 6 0.07118852111898377 1105 7 -0.20055278155601997 1105 8 -0.08242576317231709 1105 9 0.06632696411404737 1105 10-11 0.17080189413527336 1105 12-13 0.1493080410740859 1105 14-15 0.10426224972759002 1105 16-17 0.1216410897025284 1105 18-19 0.10234388843323217 1105 20-21 0.011773113121329004 1105 22-23 -0.16145114839254404 1105 24-25 0.01759580898808366 1105 26-27 -0.06116072865405897 1105 28-29 0.16035540595389364 1105 30-31 0.12279927770777732 1105 32-33 0.10564583749305001 1105 34-35 0.1658770299266905 1105 36-37 0.19020157999145937 1105 38-39 0.2955271324767068 1105 40-41 0.28708648248391455 1105 42-43 0.26796906483811256 1105 44-45 0.2863401582760261 1105 46-47 0.25293856581900087 1105 48-49 0.14955484714796796 1105 50-51 0.15011998904045498 1105 52-53 0.17586376550652005 1105 54-55 0.17931652624577765 1105 56-57 0.05578220742984996 1105 58-59 0.06207463157647197 1105 60-61 -0.045162504054985675 1105 62-63 0.051707205809151446 1105 64-65 0.18608960213349235 1105 66-67 0.15265538385771293 1105 68-69 0.14607263345114774 1105 70-71 0.18562276955779922 1105 72-73 0.11788431431292423 1105 74-75 6.250937943157453E-4 1105 76-77 0.06530929729977686 1105 78-79 0.017747245251538146 1105 80-81 0.1132636320441236 1105 82-83 0.19099373010056553 1105 84-85 0.1638886027387052 1105 86-87 0.16922714090833324 1105 88-89 0.19844607047905427 1105 90-91 0.16979995758764588 1105 92-93 0.2161527475611429 1105 94-95 0.17731041528027447 1105 96-97 0.19185640522265146 1105 98-99 0.18000855742108612 1105 100-101 0.20459554254880885 1105 102-103 0.18097860298846413 1105 104-105 0.08625439610728591 1105 106-107 0.1053386335096711 1105 108-109 0.1114404740951116 1105 110-111 0.09462908611986975 1105 112-113 0.11182129297341348 1105 114-115 0.0275877131720641 1105 116-117 -0.08944979759429472 1105 118-119 -0.026163168202405274 1105 120-121 0.07894420101919764 1105 122-123 0.023534119644555318 1105 124-125 -0.029474283225709996 1106 1 -0.2801354354179999 1106 2 -0.33165604238775614 1106 3 -0.07360882136588742 1106 4 -0.18369235506518322 1106 5 0.060625650081931326 1106 6 -0.2386962233662473 1106 7 -0.23460432971892686 1106 8 -0.3112229234419601 1106 9 -0.3076632339022751 1106 10-11 -0.1910018666785831 1106 12-13 -0.11391290627857842 1106 14-15 -0.3990966754163665 1106 16-17 -0.37030173883295703 1106 18-19 -0.4127292477397049 1106 20-21 -0.5566391816623408 1106 22-23 -0.5017719666161753 1106 24-25 -3.910216626932325 1106 26-27 -1.1646918720279302 1106 28-29 -0.8137139505623168 1106 30-31 -0.7293165726503261 1106 32-33 -5.366140900086826 1106 34-35 -1.3325834434725365 1106 36-37 -0.6973889627384224 1106 38-39 -0.16672086731638558 1106 40-41 -0.8115062604273788 1106 42-43 -0.5445709993384682 1106 44-45 -0.5116347881049492 1106 46-47 -0.4300404169988923 1106 48-49 -0.375487226010371 1106 50-51 -0.2894881717667559 1106 52-53 -0.23703348177281214 1106 54-55 -0.38672909565184455 1106 56-57 -0.1509736518693856 1106 58-59 -0.004665128158542586 1106 60-61 0.016474441997154088 1106 62-63 0.004103112843637291 1106 64-65 0.0338071679311156 1106 66-67 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118-119 -1.2857494831314291 2216 120-121 -1.2917019349199919 2216 122-123 -1.2395682636584162 2216 124-125 -1.2396406926386732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48548.0 3 8655.0 4 7201.0 5 6419.0 6 6545.0 7 5425.0 8 4811.0 9 5121.0 10 6092.0 11 7015.0 12 7673.0 13 8232.0 14 8448.0 15 7977.0 16 7316.0 17 6565.0 18 5753.0 19 5034.0 20 4925.0 21 4856.0 22 5747.0 23 8692.0 24 13661.0 25 19468.0 26 23787.0 27 19308.0 28 20878.0 29 25594.0 30 29803.0 31 36546.0 32 46994.0 33 62027.0 34 86029.0 35 120879.0 36 198124.0 37 298208.0 38 117760.0 39 530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.0998499205214 18.52513429472977 8.923874269979283 20.45114151476955 2 17.777910433140168 22.994403984558918 38.151860797585265 21.075824784715653 3 16.837817856765664 33.71971817835337 28.560386088921064 20.882077875959904 4 10.019217243630415 17.764049595948077 36.038569183990376 36.17816397643113 5 9.474157908754442 47.84743630168648 32.338814107764414 10.339591681794666 6 27.96508277381637 38.94383503248425 19.95036096593305 13.140721227766328 7 27.712733634667398 37.90426393312894 20.11573248514886 14.267269947054798 8 27.864404756073668 36.35429932405956 19.1115424177116 16.66975350215517 9 28.915327009103674 16.44042047580963 17.516636499657512 37.12761601542918 10-11 23.279086373436133 31.02263325825395 27.38805633173302 18.3102240365769 12-13 23.877145001180534 29.782295988829137 27.271224428647034 19.0693345813433 14-15 21.71517504843695 30.493943994143557 24.828801262517743 22.96207969490175 16-17 21.257656773721507 32.89480481660671 25.315081954791978 20.532456454879807 18-19 20.807545994357113 31.488048565724565 27.243866813153677 20.460538626764645 20-21 22.405412541759308 30.51259188960619 26.98047214324255 20.101523425391953 22-23 22.18330736456021 30.737490175530525 26.68191853312436 20.39728392678491 24-25 21.441595888589344 30.5506818229652 27.10255375083948 20.905168537605974 26-27 21.359811421114866 30.492226095674702 27.12371279986836 21.024249683342074 28-29 21.331734805631942 30.549609335238504 27.08731466479517 21.031341194334384 30-31 21.46605894343865 30.20723994748304 27.46046973673788 20.86623137234043 32-33 21.16097702842842 30.572624682863736 27.165002697810753 21.10139559089709 34-35 21.49106594144168 30.55297257293386 27.33446142707685 20.621500058547607 36-37 21.561623224254074 30.316484952940147 27.19797586776404 20.923915955041732 38-39 21.393923901829748 30.59811208699033 27.07373483501042 20.9342291761695 40-41 21.36943044419452 30.533355629988286 27.367715336514305 20.729498589302885 42-43 20.954143044923246 30.350083325839428 27.384831444550528 21.310942184686795 44-45 21.06963810045694 30.355623806318523 27.477777577940632 21.096960515283904 46-47 21.175037157109905 30.262648341438254 27.19820084585042 21.364113655601425 48-49 21.041862700566835 30.432930863538978 27.199983469056715 21.325222966837472 50-51 21.05202736832438 30.285622445699588 27.512092268601428 21.150257917374603 52-53 21.115411004640595 30.31176205197909 27.481791781323057 21.09103516205726 54-55 20.77089007166968 30.313721783579687 27.513097730441032 21.4022904143096 56-57 21.148036253776432 30.82125862672654 26.907580422042326 21.1231246974547 58-59 21.388717009131504 30.287789853367116 27.061820461102048 21.261672676399336 60-61 21.379702509395358 30.172975583361616 27.45912288066357 20.988199026579455 62-63 21.056220587301137 30.38344707455337 27.50219841866922 21.05813391947627 64-65 20.729549929111396 30.34127936232783 27.732387386611716 21.196783321949063 66-67 21.064923058319856 30.718201431305815 27.082614249230275 21.13426126114405 68-69 20.9129265507667 30.471077049295754 27.40417435812662 21.21182204181093 70-71 20.803268852228165 30.5018765746543 27.36925203346609 21.32560253965144 72-73 20.96937688053398 30.867179915562275 27.0648420935014 21.098601110402342 74-75 20.531752385121557 30.81165307712441 27.397825877325232 21.258768660428796 76-77 20.631940742736244 30.700769874521818 27.285563790833216 21.381725591908722 78-79 20.97451254856912 30.773784423136235 27.07373016194936 21.177972866345282 80-81 20.93226244290215 30.652242376143057 26.978272873484133 21.43722230747066 82-83 20.872517686531296 30.807932382500514 27.107727889157264 21.21182204181093 84-85 20.80168095009957 30.59449336543598 27.364649230623233 21.23917645384122 86-87 20.54868955921694 30.541491710994723 27.478369145912744 21.431449583875594 88-89 20.867898810573852 30.338727964926793 27.428856565913218 21.364516658586137 90-91 21.127961363512 30.3828405747411 27.309233105816023 21.179964955930878 92-93 20.97664125928956 30.446951210412344 27.138622185197697 21.437785345100398 94-95 20.573906900941765 30.242136226452494 27.544416386909788 21.639540485695953 96-97 20.728686626025482 30.357975828815697 27.413090919944576 21.500246625214242 98-99 20.403241760996085 30.596482339566005 27.163505047888258 21.836770851549648 100-101 20.300948965413788 30.786406440673687 27.340145496040012 21.57249909787251 102-103 20.368635626486174 30.72712300124978 27.153155180238613 21.751086192025433 104-105 20.338043906676308 30.664761008238724 27.386684116069137 21.61051096901583 106-107 20.193137407591795 30.68034710548359 27.49112318708866 21.635392299835953 108-109 20.189326234173546 31.272043767437463 26.95147491626382 21.58715508212517 110-111 20.12436573475276 31.51821862348178 26.865983484230426 21.491432157535034 112-113 20.376186824506863 31.31020221361999 26.93469805715125 21.37891290472189 114-115 20.06994286099134 31.493152655900104 26.836583659743557 21.600320823365003 116-117 20.002104646868954 31.29567801198883 26.977248767346595 21.724968573795618 118-119 20.14708110813653 31.20498903282194 26.80212241299347 21.845807446048067 120-121 20.225778517251765 31.191775511820634 26.898034607229487 21.68441136369811 122-123 20.29032591351066 31.085880907828123 26.805611360479396 21.818181818181817 124-125 20.262911279592636 31.042748242914932 26.50365805079217 22.19068242670027 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3517.0 1 4045.5 2 4779.0 3 4150.5 4 2785.0 5 2004.0 6 1591.5 7 1440.0 8 1464.0 9 1514.0 10 1326.0 11 1093.0 12 1048.0 13 1032.0 14 1095.5 15 1174.5 16 1296.5 17 1461.0 18 1722.5 19 2036.5 20 2489.0 21 3302.0 22 4469.5 23 5722.0 24 7073.5 25 8914.0 26 11043.0 27 13086.0 28 15362.0 29 18160.5 30 21408.0 31 24283.5 32 27082.5 33 30938.0 34 34894.0 35 36434.0 36 38617.0 37 42519.0 38 43470.0 39 43613.5 40 43979.0 41 43864.5 42 43284.0 43 43364.5 44 44973.5 45 45631.0 46 45145.0 47 44439.0 48 44330.0 49 44079.5 50 42706.0 51 39624.5 52 36795.5 53 36311.0 54 34455.0 55 30527.5 56 27214.5 57 24344.0 58 22157.0 59 19975.0 60 16991.0 61 14149.0 62 11544.0 63 8832.5 64 6379.5 65 4681.5 66 3603.5 67 2785.0 68 2037.5 69 1425.5 70 1087.0 71 895.5 72 781.0 73 737.0 74 558.0 75 375.5 76 268.5 77 168.0 78 123.0 79 100.5 80 70.0 81 34.5 82 19.5 83 17.0 84 13.0 85 8.5 86 9.5 87 10.0 88 10.0 89 8.0 90 3.0 91 1.5 92 1.5 93 3.0 94 4.0 95 1.5 96 1.0 97 1.0 98 0.0 99 1.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.826591134859786E-4 2 1.5306364539439143E-4 3 3.0612729078878287E-4 4 5.3572275888037E-4 5 0.001377572808549523 6 9.949136950635443E-4 7 9.183818723663487E-4 8 0.0016837000993383058 9 0.002372486503613067 10-11 0.002219422858218676 12-13 0.002487284237658861 14-15 0.003864857046208384 16-17 0.003176070641933622 18-19 0.0034821979327224056 20-21 0.003903122957556982 22-23 0.09428720556294513 24-25 0.0029464751738420354 26-27 0.002372486503613067 28-29 0.0020663592128242845 30-31 0.0015689023652925124 32-33 0.002372486503613067 34-35 0.0013010409858523271 36-37 9.183818723663487E-4 38-39 0.001377572808549523 40-41 0.0017219660106869037 42-43 0.002181156946870078 44-45 0.0020280933014756868 46-47 0.002449018326310263 48-49 4.2092502483457645E-4 50-51 0.0019898273901270886 52-53 0.001875029656081295 54-55 0.0020280933014756868 56-57 0.0017984978333840996 58-59 0.0011862432518065335 60-61 0.0012627750745037294 62-63 0.001913295567429893 64-65 0.001415838719898121 66-67 5.3572275888037E-4 68-69 1.5306364539439143E-4 70-71 7.653182269719573E-4 72-73 1.913295567429893E-4 74-75 3.0612729078878287E-4 76-77 3.4439320213738075E-4 78-79 2.2959546809158717E-4 80-81 5.739886702289679E-4 82-83 1.5306364539439143E-4 84-85 3.0612729078878287E-4 86-87 4.2092502483457645E-4 88-89 5.739886702289679E-4 90-91 4.2092502483457645E-4 92-93 3.4439320213738075E-4 94-95 4.2092502483457645E-4 96-97 5.739886702289679E-4 98-99 8.035841383205551E-4 100-101 9.566477837149465E-4 102-103 0.0013010409858523271 104-105 8.418500496691529E-4 106-107 8.801159610177508E-4 108-109 9.566477837149465E-4 110-111 0.0012245091631551315 112-113 6.122545815775657E-4 114-115 6.122545815775657E-4 116-117 0.0010331796064121422 118-119 0.0015306364539439145 120-121 8.801159610177508E-4 122-123 8.418500496691529E-4 124-125 5.739886702289679E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1306646.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.071195210040756 #Duplication Level Percentage of deduplicated Percentage of total 1 58.019320520233286 19.767875953987915 2 11.55338636128229 7.872753641045425 3 8.383693276712398 8.569273506359231 4 6.051548456562787 8.247339551462861 5 4.377302687362879 7.456996717728831 6 2.936958341148215 6.003940857901099 7 2.066011637185428 4.9274040057732424 8 1.4165139925622843 3.8609859806675044 9 1.0102362223541137 3.0977959986073036 >10 3.959978117740317 21.975003699863354 >50 0.1625937685455605 3.7554911897177363 >100 0.059985317650856386 3.45726289553348 >500 0.0015726458743171646 0.3475170537053843 >1k 8.986547853240941E-4 0.6603589476467733 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4424 0.33857678361239385 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1438 0.11005276103856745 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1434 0.10974663374777867 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1331 0.1018638560099675 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.653182269719572E-5 0.0 5 0.0 0.0 0.0 7.653182269719572E-5 0.0 6 0.0 0.0 0.0 7.653182269719572E-5 0.0 7 0.0 0.0 0.0 7.653182269719572E-5 0.0 8 0.0 0.0 0.0 7.653182269719572E-5 0.0 9 0.0 0.0 0.0 7.653182269719572E-5 0.0 10-11 0.0 0.0 0.0 1.9132955674298929E-4 0.0 12-13 0.0 0.0 0.0 2.2959546809158717E-4 0.0 14-15 0.0 0.0 0.0 2.2959546809158717E-4 0.0 16-17 0.0 0.0 0.0 2.2959546809158717E-4 0.0 18-19 0.0 0.0 0.0 3.0612729078878287E-4 0.0 20-21 0.0 0.0 0.0 3.4439320213738075E-4 0.0 22-23 0.0 0.0 0.0 4.5919093618317433E-4 0.0 24-25 0.0 0.0 0.0 0.0012245091631551315 0.0 26-27 0.0 0.0 0.0 0.0014158387198981207 0.0 28-29 0.0 0.0 0.0 0.0018367637447326973 0.0 30-31 0.0 0.0 0.0 0.004859770741271929 0.0 32-33 0.0 0.0 0.0 0.014043589464935415 0.0 34-35 0.0 0.0 0.0 0.023992726415570858 0.0 36-37 0.0 0.0 0.0 0.03975828189119318 0.0 38-39 0.0 0.0 0.0 0.06864904495938456 0.0 40-41 0.0 0.0 0.0 0.09968269906309743 0.0 42-43 0.0 0.0 0.0 0.13733635583011772 0.0 44-45 0.0 0.0 0.0 0.185934063242837 0.0 46-47 0.0 0.0 0.0 0.24176402790044127 0.0 48-49 0.0 0.0 0.0 0.3001960745297502 0.0 50-51 0.0 0.0 0.0 0.3577097392866928 0.0 52-53 0.0 0.0 0.0 0.42658837971416896 0.0 54-55 0.0 0.0 0.0 0.5043829774858684 0.0 56-57 0.0 0.0 0.0 0.6053667175348181 0.0 58-59 0.0 0.0 0.0 0.7284681543432574 0.0 60-61 0.0 0.0 0.0 0.8448730566656921 0.0 62-63 0.0 0.0 0.0 0.9633060522896026 0.0 64-65 0.0 0.0 0.0 1.0932953531407894 0.0 66-67 0.0 0.0 0.0 1.263195999528564 0.0 68-69 0.0 0.0 0.0 1.477867762194198 0.0 70-71 0.0 0.0 0.0 1.696901838753572 0.0 72-73 0.0 0.0 0.0 1.8966881619046014 0.0 74-75 0.0 0.0 0.0 2.118821777283212 0.0 76-77 0.0 0.0 0.0 2.3870275499255342 0.0 78-79 0.0 0.0 0.0 2.7285125428004218 0.0 80-81 0.0 0.0 0.0 3.064219383061671 0.0 82-83 0.0 0.0 0.0 3.3959848344540142 0.0 84-85 0.0 0.0 0.0 3.7340641612188765 0.0 86-87 0.0 0.0 0.0 4.102832748885314 0.0 88-89 0.0 0.0 0.0 4.5541791732420265 0.0 90-91 0.0 0.0 0.0 5.056189664224281 0.0 92-93 0.0 0.0 0.0 5.535776331156258 0.0 94-95 0.0 0.0 0.0 6.038743469922228 0.0 96-97 0.0 0.0 0.0 6.574810622004736 0.0 98-99 0.0 0.0 0.0 7.186529480823421 0.0 100-101 0.0 0.0 0.0 7.840991362618491 0.0 102-103 0.0 0.0 0.0 8.493731278402873 0.0 104-105 0.0 0.0 0.0 9.179915600705929 0.0 106-107 0.0 0.0 0.0 9.874594955328375 0.0 108-109 0.0 0.0 0.0 10.597055361589902 0.0 110-111 0.0 0.0 0.0 11.368649198023029 0.0 112-113 0.0 0.0 0.0 12.119617708239263 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15540 0.0 56.77505 1 TACATGG 15640 0.0 55.156746 2 GGTATCA 3070 0.0 53.29196 1 ACATGGG 15865 0.0 52.049526 3 CATGGGG 3830 0.0 48.309105 4 CATGGGA 11825 0.0 48.198303 4 ATGGGAG 4180 0.0 46.25668 5 ATGGGAT 4250 0.0 41.295284 5 ATGGGGG 2325 0.0 40.941563 5 GTATCAA 4290 0.0 37.99799 2 ATGGGCG 205 1.8189894E-12 34.825294 5 ATGGGAC 2130 0.0 33.79661 5 TACCTGG 1145 0.0 33.253845 2 GAGTACA 8855 0.0 33.1227 1 CGGGAGT 380 0.0 32.877827 4 AGTACAT 8610 0.0 32.614166 2 GAACAAA 2710 0.0 32.27122 1 CATGGGT 1020 0.0 32.079674 4 ATCAACG 5185 0.0 32.012737 4 TCAACGC 5180 0.0 31.928785 5 >>END_MODULE