FastQCFastQC Report
Thu 26 May 2016
SRR936250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306646
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125390.9596325248001372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105960.8109311932994859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102090.7813133779156711No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33850.2590602198300075No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.2362537366662432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25820.19760516620415933No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.13706849445067754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15640.11969577069841411No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13250.10140466507378434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.077.430541
GTACATG132000.050.9049641
TACATGG135200.049.2752462
ACATGGG132050.049.1882973
GTATCAA95200.048.4037972
CATGGGA98400.045.4381074
CATGGGG33250.043.3310434
ATGGGAG35300.043.007175
ATGGGAT39750.040.8884775
TACCTGG10200.037.355562
CGGGAGT3850.037.1129884
ATCAACG132800.036.4475144
TCAACGC134550.035.9734655
CAACGCA134900.035.9008946
TATCAAC137300.035.4697573
AACGCAG137000.035.3940167
GTCGGGA4100.033.397922
TGGGATA11800.033.277816
AGTACAT78600.032.9489942
TATCACG1451.09228495E-732.847132