##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936250_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1306646 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50922514590792 31.0 31.0 34.0 30.0 34.0 2 31.290755108881825 31.0 31.0 34.0 28.0 34.0 3 31.697462817013943 31.0 31.0 34.0 30.0 34.0 4 35.5676449474456 37.0 35.0 37.0 33.0 37.0 5 35.18431618051102 37.0 35.0 37.0 33.0 37.0 6 35.41457058759603 37.0 35.0 37.0 33.0 37.0 7 35.24169055735065 37.0 35.0 37.0 32.0 37.0 8 35.34208729831951 37.0 35.0 37.0 33.0 37.0 9 36.981682873555656 39.0 37.0 39.0 33.0 39.0 10-11 36.881001051547244 39.0 37.0 39.0 32.0 39.0 12-13 36.80571401894622 39.0 37.0 39.0 32.0 39.0 14-15 37.893036063325496 40.0 37.0 41.0 32.5 41.0 16-17 37.81679200028164 40.0 37.0 41.0 32.0 41.0 18-19 38.0046818342535 40.0 37.0 41.0 33.0 41.0 20-21 38.06573853974221 40.0 37.0 41.0 33.0 41.0 22-23 37.94514926001381 40.0 37.0 41.0 33.0 41.0 24-25 37.88728010494043 40.0 37.0 41.0 33.0 41.0 26-27 37.68450062220372 40.0 36.5 41.0 32.0 41.0 28-29 37.50709182135024 40.0 36.0 41.0 32.0 41.0 30-31 37.220375679411255 39.0 36.0 41.0 31.5 41.0 32-33 36.89373518152583 39.0 35.0 41.0 31.0 41.0 34-35 36.61426889915096 39.0 35.0 41.0 30.5 41.0 36-37 36.333542902974486 39.0 35.0 41.0 30.0 41.0 38-39 36.26756137469521 39.0 35.0 41.0 30.0 41.0 40-41 36.226921446206546 39.0 35.0 41.0 30.0 41.0 42-43 36.06947482332629 39.0 35.0 41.0 30.0 41.0 44-45 35.789972188335625 39.0 35.0 41.0 28.5 41.0 46-47 35.32733617215374 39.0 35.0 41.0 25.0 41.0 48-49 34.941574458575616 39.0 35.0 41.0 23.5 41.0 50-51 34.7660031867851 39.0 35.0 41.0 23.0 41.0 52-53 34.6521728149782 39.0 34.5 41.0 23.0 41.0 54-55 34.49721041506268 39.0 34.0 41.0 23.0 41.0 56-57 34.31722325710254 38.0 34.0 40.5 22.0 41.0 58-59 34.10774800519804 38.0 34.0 40.0 22.0 41.0 60-61 33.89567067132184 38.0 34.0 40.0 21.0 41.0 62-63 33.688328361315925 37.5 33.0 40.0 20.5 41.0 64-65 33.45162844412335 37.0 33.0 40.0 20.0 41.0 66-67 33.172033970945456 36.5 33.0 40.0 19.0 41.0 68-69 32.872152824866106 36.0 33.0 39.0 18.0 41.0 70-71 32.558645187755516 36.0 32.5 39.0 17.5 41.0 72-73 32.277893170759334 35.0 32.0 39.0 16.0 40.0 74-75 32.06660794124805 35.0 32.0 37.5 14.5 40.0 76-77 31.749344887597715 35.0 32.0 37.0 13.0 39.0 78-79 31.407916910930737 35.0 32.0 37.0 10.5 39.0 80-81 31.111412731527896 35.0 32.0 36.0 8.0 39.0 82-83 30.832050149772776 35.0 32.0 36.0 7.0 37.0 84-85 30.56239716036325 35.0 31.5 35.5 6.5 37.0 86-87 30.349601957990153 35.0 31.0 35.0 2.0 37.0 88-89 30.166993585102624 35.0 31.0 35.0 2.0 36.0 90-91 30.005509525915972 35.0 31.0 35.0 2.0 36.0 92-93 29.85191704562674 34.5 31.0 35.0 2.0 36.0 94-95 29.71585341400808 34.0 31.0 35.0 2.0 35.5 96-97 29.631410114139562 34.0 31.0 35.0 2.0 35.0 98-99 29.536084371742614 34.0 31.0 35.0 2.0 35.0 100-101 29.440445614190835 34.0 31.0 35.0 2.0 35.0 102-103 28.436357666881467 33.5 29.0 34.5 2.0 35.0 104-105 29.1402158656591 34.0 30.5 35.0 2.0 35.0 106-107 29.261730797783024 34.0 31.0 35.0 2.0 35.0 108-109 29.21459905743407 34.0 31.0 35.0 2.0 35.0 110-111 29.165695222730562 34.0 31.0 35.0 2.0 35.0 112-113 29.068270212437035 34.0 31.0 35.0 2.0 35.0 114-115 29.026119163109215 34.0 31.0 35.0 2.0 35.0 116-117 28.91532825264073 34.0 31.0 35.0 2.0 35.0 118-119 28.778911044001205 34.0 31.0 35.0 2.0 35.0 120-121 28.57168238375199 34.0 30.0 35.0 2.0 35.0 122-123 28.348342244188558 34.0 30.0 35.0 2.0 35.0 124-125 26.373318021866673 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23138196580292814 1101 2 0.616233192299088 1101 3 0.3542201991547742 1101 4 0.23017293973654773 1101 5 0.4705481897358865 1101 6 0.22036590503319786 1101 7 0.36094595076463776 1101 8 0.2877412408529665 1101 9 0.2143465163894831 1101 10-11 0.25718103824013383 1101 12-13 0.3734196479932166 1101 14-15 0.43562530038602887 1101 16-17 0.45195148436815913 1101 18-19 0.4550799869423727 1101 20-21 0.3651683287424916 1101 22-23 0.33443364321804836 1101 24-25 0.3917964805212577 1101 26-27 0.45773593046596517 1101 28-29 0.5181545878652827 1101 30-31 0.31740968718913365 1101 32-33 0.41614814820521673 1101 34-35 0.45125368667240906 1101 36-37 0.4865143417100626 1101 38-39 0.4175759753117205 1101 40-41 0.2932926026211504 1101 42-43 0.35081698627499946 1101 44-45 0.3675713138543628 1101 46-47 0.47461484741927507 1101 48-49 0.5067884549694242 1101 50-51 0.5366145486062806 1101 52-53 0.47297303225148113 1101 54-55 0.4212483128699205 1101 56-57 0.3030561349934544 1101 58-59 0.6442853942942648 1101 60-61 0.505691610393356 1101 62-63 0.36500823246797864 1101 64-65 0.42366658024636195 1101 66-67 0.47377892356880125 1101 68-69 0.4432101712912342 1101 70-71 0.2760137884321878 1101 72-73 0.42271000097692024 1101 74-75 0.5083155023774708 1101 76-77 0.5247616077726498 1101 78-79 0.5816542150293564 1101 80-81 0.5658818445555589 1101 82-83 0.44183385818560694 1101 84-85 0.3742759074793369 1101 86-87 0.40874284821840945 1101 88-89 0.3899622795481008 1101 90-91 0.4952674283046292 1101 92-93 0.4080651825170065 1101 94-95 0.39975634389827874 1101 96-97 0.36999835020132466 1101 98-99 0.42574499342920546 1101 100-101 0.5048571182411052 1101 102-103 0.5646032629161795 1101 104-105 0.419889290303221 1101 106-107 0.5300604476384407 1101 108-109 0.4255125657278782 1101 110-111 0.513976265336904 1101 112-113 0.4629412205988963 1101 114-115 0.4329762753608115 1101 116-117 0.5929620476941686 1101 118-119 0.6635733018562 1101 120-121 0.6200745125970926 1101 122-123 0.5030780390108305 1101 124-125 0.5411394127131999 1102 1 0.18154217318001287 1102 2 0.6059745531644971 1102 3 0.2841245299652151 1102 4 0.21153986219033527 1102 5 0.41608223389336985 1102 6 0.32652901921638744 1102 7 0.40388699066421907 1102 8 0.34189356257209624 1102 9 0.39704522181612134 1102 10-11 0.4397811489824548 1102 12-13 0.4300107206099426 1102 14-15 0.40722056717030597 1102 16-17 0.43916580237686986 1102 18-19 0.4684961717569749 1102 20-21 0.47468557897867214 1102 22-23 0.4999557889258881 1102 24-25 0.3915633514134953 1102 26-27 0.4422420693328064 1102 28-29 0.4135108860529897 1102 30-31 0.4793777520953455 1102 32-33 0.33482562330915755 1102 34-35 0.42649156581568803 1102 36-37 0.5114916218049785 1102 38-39 0.3971850851251091 1102 40-41 0.3081902234822991 1102 42-43 0.29355120495585396 1102 44-45 0.4178677807623714 1102 46-47 0.41629387535955686 1102 48-49 0.1606184451482946 1102 50-51 0.17295434494296558 1102 52-53 0.25361699091311607 1102 54-55 0.2258203508424117 1102 56-57 0.1976473959344105 1102 58-59 0.19875542330721885 1102 60-61 0.3023813447819066 1102 62-63 0.3795704157640074 1102 64-65 0.2562190229146921 1102 66-67 0.3325159819682426 1102 68-69 0.31547782267480073 1102 70-71 0.21749981997338352 1102 72-73 0.2683441492877634 1102 74-75 0.24972955860111767 1102 76-77 0.15942697166370223 1102 78-79 0.2298262120784642 1102 80-81 0.1757359699464338 1102 82-83 0.2779358292886194 1102 84-85 0.13936442825477968 1102 86-87 0.24283159493501572 1102 88-89 0.2713308312700562 1102 90-91 0.1842890120347569 1102 92-93 0.21518474899750117 1102 94-95 0.19523958124455731 1102 96-97 0.31772615603472687 1102 98-99 0.34668810968731023 1102 100-101 0.3718265332835138 1102 102-103 0.4131105537714568 1102 104-105 0.3579525408896984 1102 106-107 0.24993533901057674 1102 108-109 0.3080307099051751 1102 110-111 0.38276135599968697 1102 112-113 0.35770918525719253 1102 114-115 0.3203988761376415 1102 116-117 0.2852793055016569 1102 118-119 0.25985730054990697 1102 120-121 0.3223565422722956 1102 122-123 0.3766663560079522 1102 124-125 0.20255184925260394 1103 1 0.0021905143090883428 1103 2 0.5405473769263622 1103 3 0.25439737966912546 1103 4 0.13033028029163063 1103 5 0.24268698331635363 1103 6 0.057854863007086976 1103 7 0.1838409250368045 1103 8 0.13995967493337247 1103 9 0.07959811005841289 1103 10-11 0.203370592598354 1103 12-13 0.17830187484960902 1103 14-15 0.3067342791047736 1103 16-17 0.3333526839114498 1103 18-19 0.2414390713330974 1103 20-21 0.22189418381449144 1103 22-23 0.24108615092841035 1103 24-25 0.17916171699300776 1103 26-27 0.14447821641155656 1103 28-29 0.12335101193038156 1103 30-31 0.0673456307352609 1103 32-33 0.214306443497847 1103 34-35 0.24079914110788536 1103 36-37 0.052736133582598654 1103 38-39 0.1848536159437586 1103 40-41 0.3096183486217683 1103 42-43 0.2901821995194709 1103 44-45 0.3598161883097646 1103 46-47 0.4400449281364587 1103 48-49 0.4215690552636886 1103 50-51 0.30713856306235243 1103 52-53 0.18901688146488738 1103 54-55 0.1746678629238403 1103 56-57 0.24907959018042902 1103 58-59 0.2829760247283275 1103 60-61 0.22348960234752724 1103 62-63 0.06852242154714361 1103 64-65 0.19883459687708438 1103 66-67 0.17939251907026232 1103 68-69 0.15167563996308786 1103 70-71 0.1797078436787345 1103 72-73 0.2794979164093334 1103 74-75 0.2731431499897923 1103 76-77 0.3710350706415575 1103 78-79 0.4034916226918881 1103 80-81 0.33346301236943887 1103 82-83 0.2559318671005215 1103 84-85 0.26333363764766204 1103 86-87 0.2982470984457848 1103 88-89 0.3377110853398193 1103 90-91 0.2338981033495635 1103 92-93 0.23456002061931258 1103 94-95 0.28499500288155843 1103 96-97 0.3381554575159065 1103 98-99 0.36580109696281937 1103 100-101 0.3696240846121306 1103 102-103 0.2013771597237124 1103 104-105 0.18387640110431036 1103 106-107 0.29537114986896285 1103 108-109 0.23312036747732634 1103 110-111 0.21144325902416483 1103 112-113 0.2301812378935324 1103 114-115 0.3961123002991549 1103 116-117 0.36298050611481614 1103 118-119 0.36268350387779336 1103 120-121 0.3461905284526061 1103 122-123 0.30071057538268064 1103 124-125 0.4229369275405439 1104 1 0.03944430169852353 1104 2 0.4214868389835651 1104 3 0.14175681491244418 1104 4 0.09355449202370636 1104 5 0.41751910945070847 1104 6 0.14947136129748628 1104 7 0.23731366760341643 1104 8 0.1534993947943093 1104 9 0.2249399870877511 1104 10-11 0.10698773970437969 1104 12-13 0.20642020905854963 1104 14-15 0.18668078052441928 1104 16-17 0.28577382841073273 1104 18-19 0.23841389671269297 1104 20-21 0.21305837723044618 1104 22-23 0.3163157484043424 1104 24-25 0.22993297503501964 1104 26-27 0.07412011899160831 1104 28-29 0.14260985491412725 1104 30-31 0.0637089617431883 1104 32-33 -0.014711117586998057 1104 34-35 0.12108763439149328 1104 36-37 0.17998760992523444 1104 38-39 0.195123778750542 1104 40-41 0.15016257861255156 1104 42-43 0.17240565621390402 1104 44-45 0.2858318287855255 1104 46-47 0.3832627952555754 1104 48-49 0.3000015339403319 1104 50-51 0.19474202188121836 1104 52-53 0.3337716664801178 1104 54-55 0.33964861377003075 1104 56-57 0.2776019987218348 1104 58-59 0.37078970268036215 1104 60-61 0.2544156431080751 1104 62-63 0.24572480608811276 1104 64-65 0.28522827293435427 1104 66-67 0.20630831945322825 1104 68-69 0.23718042426060038 1104 70-71 0.2815248862852471 1104 72-73 0.1795551901779504 1104 74-75 0.16593246864725586 1104 76-77 0.21498809466783442 1104 78-79 0.28254995006817296 1104 80-81 0.11089556855945304 1104 82-83 0.06255789666469624 1104 84-85 0.10208680636556267 1104 86-87 0.1570471627531731 1104 88-89 0.05090156440115834 1104 90-91 0.0804334709782566 1104 92-93 0.16949364620676377 1104 94-95 0.12187600837352619 1104 96-97 0.21473084501098683 1104 98-99 0.15095628160254293 1104 100-101 0.20150655424555097 1104 102-103 0.24214309231004805 1104 104-105 0.2743032844639153 1104 106-107 0.18774634616439556 1104 108-109 0.13233719534309785 1104 110-111 0.15378048812211276 1104 112-113 0.20276590689708485 1104 114-115 0.07536089429273218 1104 116-117 0.15854652597546348 1104 118-119 0.21970829667306901 1104 120-121 0.2331204104313187 1104 122-123 0.11768463998289747 1104 124-125 0.17056164511913963 1105 1 0.09427378799044561 1105 2 0.5633320680330911 1105 3 0.24686847385876476 1105 4 0.13100334353973153 1105 5 0.309064154825343 1105 6 0.17953850863325727 1105 7 0.18425057462339822 1105 8 0.0886001533209324 1105 9 0.023258770893590963 1105 10-11 0.0765697526985818 1105 12-13 0.09651335042443066 1105 14-15 0.030367870937858754 1105 16-17 0.24248312115923198 1105 18-19 0.22982489131501183 1105 20-21 0.08364028328421824 1105 22-23 0.15846557007647988 1105 24-25 0.07991524543671602 1105 26-27 -0.014965718530227434 1105 28-29 0.027145845998674645 1105 30-31 -0.006618444838714765 1105 32-33 -0.06568089847166902 1105 34-35 -0.038766739003229134 1105 36-37 0.09986225509874203 1105 38-39 0.004184682265886863 1105 40-41 0.04521883490937029 1105 42-43 -0.017093262186790525 1105 44-45 0.14984018139627153 1105 46-47 0.2158006418504641 1105 48-49 0.1518796518637373 1105 50-51 0.10544957356439966 1105 52-53 0.08022668489451235 1105 54-55 0.1408744888523401 1105 56-57 0.16262297405610582 1105 58-59 0.13554068156057042 1105 60-61 0.03216363703685943 1105 62-63 -0.01718848585740318 1105 64-65 -0.04662665580016778 1105 66-67 -0.04288915825344475 1105 68-69 -0.06565870355573367 1105 70-71 -0.02085130201997032 1105 72-73 -0.05277290307410709 1105 74-75 0.06060388009213824 1105 76-77 0.08630474716958147 1105 78-79 0.11919374649111347 1105 80-81 0.09581321504439799 1105 82-83 0.009270369484003282 1105 84-85 0.023519123389540653 1105 86-87 0.16825245649227938 1105 88-89 0.15961167515017394 1105 90-91 0.13052028972548158 1105 92-93 0.04722719379619633 1105 94-95 0.13257192793930983 1105 96-97 0.15649169430565024 1105 98-99 0.1617483537516904 1105 100-101 0.14597749928004689 1105 102-103 0.09981217545529475 1105 104-105 0.043767147039826426 1105 106-107 0.07066181444154651 1105 108-109 0.07275495721113856 1105 110-111 0.18687526924130893 1105 112-113 0.15023235668104462 1105 114-115 0.22171521046493936 1105 116-117 0.2251415706934523 1105 118-119 0.31087759651341074 1105 120-121 0.23384483806547962 1105 122-123 0.20366732876559368 1105 124-125 0.4371545322669661 1106 1 0.20292321651595557 1106 2 0.6554330288343948 1106 3 0.3674969757218065 1106 4 0.2532732065964467 1106 5 0.4348338026670078 1106 6 0.21287282721435474 1106 7 0.3581891394991956 1106 8 0.21260282087924764 1106 9 0.1936208863929565 1106 10-11 0.16426157581951628 1106 12-13 0.15604802406418372 1106 14-15 0.193524438507076 1106 16-17 0.21186531539220965 1106 18-19 0.08255359657014338 1106 20-21 0.004550596796207174 1106 22-23 0.01184569344181341 1106 24-25 0.09683872309037866 1106 26-27 0.13139068130055165 1106 28-29 0.1258909944231661 1106 30-31 0.17052109764917134 1106 32-33 -0.039336220010355305 1106 34-35 -0.05871962260790298 1106 36-37 0.08492235829113781 1106 38-39 0.01945040358732797 1106 40-41 -0.04700520488971449 1106 42-43 -0.09647298098476398 1106 44-45 -0.07912647227743719 1106 46-47 -0.07223261580352869 1106 48-49 -0.10630868070910537 1106 50-51 -0.16554128395213752 1106 52-53 -0.2937332629366196 1106 54-55 -0.22802236309632207 1106 56-57 -0.24350469742780234 1106 58-59 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-0.24385815023660484 2216 9 -0.4135034032192024 2216 10-11 -0.9683559007510709 2216 12-13 -0.5182668961627712 2216 14-15 -0.630747416816817 2216 16-17 -0.7756254972759251 2216 18-19 -0.848847353695156 2216 20-21 -0.6259953252183337 2216 22-23 -0.7899178852745123 2216 24-25 -0.7418061008377848 2216 26-27 -1.0846582946151315 2216 28-29 -1.8754202883983737 2216 30-31 -1.5730214551430919 2216 32-33 -1.1682763611680542 2216 34-35 -1.3801317349665752 2216 36-37 -1.3664435908712278 2216 38-39 -1.173850486908833 2216 40-41 -1.0844269352292812 2216 42-43 -0.7454448235859772 2216 44-45 -1.079508381540009 2216 46-47 -0.8065300583615311 2216 48-49 -0.7595593257673556 2216 50-51 -0.6323229581654601 2216 52-53 -0.766325381098298 2216 54-55 -0.8700808242742966 2216 56-57 -1.509569942815375 2216 58-59 -1.6432163125962589 2216 60-61 -1.1502152414471354 2216 62-63 -1.031154654126567 2216 64-65 -0.9253335990799698 2216 66-67 -1.0118688593067304 2216 68-69 -0.7961911843030123 2216 70-71 -0.7784636938919469 2216 72-73 -0.9218992685726448 2216 74-75 -0.9743462503247038 2216 76-77 -0.6999394542951798 2216 78-79 -0.698410737296399 2216 80-81 -0.7267818988333978 2216 82-83 -0.8414517997269755 2216 84-85 -0.7638547224684444 2216 86-87 -0.6836170275270099 2216 88-89 -0.7610645693404408 2216 90-91 -0.6991670862086998 2216 92-93 -0.7177837090793986 2216 94-95 -0.8642089584546113 2216 96-97 -0.9305947663469247 2216 98-99 -0.9827664969700223 2216 100-101 -1.0940901210382705 2216 102-103 -1.041061192842509 2216 104-105 -0.7449629605477561 2216 106-107 -0.8128235992045134 2216 108-109 -0.7665116195888473 2216 110-111 -0.7700999091036671 2216 112-113 -0.5769360901078286 2216 114-115 -0.7456271086186952 2216 116-117 -0.7741173045593861 2216 118-119 -0.95421706871943 2216 120-121 -0.9001516407027133 2216 122-123 -1.0511143544034027 2216 124-125 -1.4180245975500014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49.0 3 78.0 4 191.0 5 434.0 6 1118.0 7 2036.0 8 3351.0 9 5414.0 10 8981.0 11 11513.0 12 11861.0 13 14776.0 14 20808.0 15 15387.0 16 7529.0 17 6338.0 18 5901.0 19 5712.0 20 5769.0 21 5820.0 22 6636.0 23 7539.0 24 8979.0 25 11021.0 26 13561.0 27 16907.0 28 20816.0 29 26362.0 30 32383.0 31 40806.0 32 50587.0 33 64204.0 34 87233.0 35 120975.0 36 194058.0 37 326094.0 38 144617.0 39 802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.04097117773374 18.244580317293053 11.320834188494986 20.393614316478224 2 18.570642478473463 22.553291143793675 37.311262525418186 21.56480385231467 3 17.80635305966574 29.969708704576448 32.60714837836721 19.6167898573906 4 10.766726412509586 16.51855207914003 37.67837654575149 35.036344962598896 5 9.204912615515159 43.7826863769652 35.041480922145446 11.970920085374194 6 28.003223520372007 38.268743026037654 18.968718382790748 14.75931507079959 7 24.81873437794169 35.97340059970337 23.05720141492034 16.150663607434605 8 28.11748553165892 33.04116034488301 20.767063152529452 18.074290970928622 9 28.067892910551134 11.078210930887172 20.344530959418236 40.50936519914345 10-11 23.96352952521188 26.37661616076581 30.004568949815024 19.65528536420729 12-13 25.52596495148648 26.2309378362617 30.324586766423344 17.91851044582848 14-15 22.322840310229395 26.700766695799782 29.579396408820752 21.39699658515007 16-17 19.791014551760767 28.92638863165693 30.027681560269574 21.254915256312728 18-19 19.10785323645425 26.078716040916973 34.92411104461346 19.889319678015315 20-21 20.325971992414164 25.185398340483957 35.612438257952036 18.87619140914984 22-23 19.974231735297852 25.678913799146823 35.27661661995675 19.070237845598577 24-25 19.478305524220026 25.73520295474061 35.43503749293994 19.351454028099425 26-27 19.27216705978513 25.763213601847784 35.55829199339377 19.406327344973313 28-29 19.51201779211814 25.626757361978687 35.465382360639374 19.3958424852638 30-31 19.838617345478422 25.190679036250064 35.680283718773104 19.29041989949841 32-33 19.36687519037291 25.809438822756892 35.256106091473896 19.567579895396307 34-35 19.8583853527107 25.986639824374574 34.98105261224457 19.173922210670156 36-37 20.07092969327576 25.68331437895191 34.61507554456219 19.63068038321014 38-39 19.98800746338335 25.923624302221103 34.42887361993991 19.659494614455635 40-41 19.946106290456637 26.01083231418456 34.431016510975425 19.612044884383376 42-43 19.802626946637563 26.03963533168224 33.968768081113595 20.188969640566594 44-45 20.012498651018223 26.210199297228332 33.548468819577806 20.228833232175646 46-47 20.28012625926317 26.5177747264102 32.52657062512725 20.675528389199382 48-49 20.52609685150892 26.985721518522304 31.800513797250996 20.68766783271778 50-51 20.442085572998344 26.91761629993946 31.8474050500667 20.79289307699549 52-53 20.464368182058227 27.059800041482628 31.81158926686724 20.6642425095919 54-55 20.135623056685002 27.186299928246832 31.820674479789524 20.85740253527864 56-57 20.574599555557086 27.365460731627888 31.263080432533958 20.79685928028107 58-59 20.730519169909805 26.83209539225703 31.52758484775429 20.90980059007887 60-61 20.852804721808514 26.86611542164581 31.72721484014456 20.553865016401122 62-63 20.532064621349473 26.72545416557288 32.02440329183462 20.718077921243015 64-65 20.372257480641885 26.605810017813102 32.27925433704073 20.742678164504287 66-67 20.574692190683923 27.157630896688957 31.61706320733959 20.650613705287526 68-69 20.557236090622613 26.889415690393783 31.666178769389138 20.887169449594474 70-71 20.331958146670335 26.85356500655704 31.648528769667777 21.165948077104847 72-73 20.51119171813241 27.110962805757023 31.273211244182004 21.10463423192857 74-75 20.20154825180712 27.251795643006655 31.389561736827805 21.15709436835842 76-77 20.115219188146696 27.177493418233023 31.40413579868977 21.303151594930508 78-79 20.38732785109957 27.167248946729984 31.03019565375446 21.415227548415984 80-81 20.24629556002928 27.15534444604737 30.8559386794312 21.742421314492155 82-83 20.27095336875993 27.139457488660852 31.03588540273548 21.553703739843744 84-85 20.08257037308584 27.12925412679669 30.855291001444158 21.932884498673317 86-87 20.004622532704037 27.0370713347104 30.946172749557455 22.01213338302811 88-89 20.263049842936184 26.954553855726292 30.912393634180024 21.870002667157497 90-91 20.34054029853145 26.985729313474096 30.80674361018177 21.866986777812684 92-93 20.225723907991526 27.060618433302807 30.48841666854808 22.225240990157584 94-95 19.924967540655018 26.782126141625678 30.75622907553719 22.536677242182108 96-97 20.00019898525175 26.800060767034573 30.74869348962349 22.451046758090186 98-99 19.851449060459313 26.85725984360441 30.5766967888583 22.71459430707798 100-101 19.848473600548584 26.888454865429345 30.593156324212462 22.66991520980961 102-103 19.789826297886897 26.677679701308744 30.443349263584622 23.089144737219733 104-105 19.856165101707663 26.48451712296529 30.564112297447572 23.09520547787947 106-107 19.68363166178328 26.466746999846553 30.746650860239782 23.102970478130384 108-109 19.834376151574023 26.73691324014999 30.127338496466688 23.3013721118093 110-111 19.672945404674003 26.90455307997967 29.956981001536775 23.46552051380955 112-113 19.565736530346854 26.596714455523145 30.19270407979726 23.64484493433274 114-115 19.344208586862585 26.675895654516907 30.06691717870071 23.912978579919795 116-117 19.020423503620414 26.640113011864948 30.19821855517959 24.141244929335052 118-119 19.100282510740538 26.576790338147983 30.105878381843386 24.2170487692681 120-121 19.13771138848718 26.496600448732348 29.944293051383795 24.421395111396677 122-123 19.10243374523991 26.440713120993074 29.68957186641476 24.767281267352256 124-125 18.714535221012447 26.190208315156077 29.66990872339807 25.425347740433406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 854.0 1 644.5 2 1296.5 3 2315.0 4 1783.5 5 1474.0 6 2274.5 7 4935.0 8 7530.0 9 8216.0 10 6569.0 11 4070.5 12 3448.0 13 3351.5 14 3390.5 15 3531.5 16 3654.0 17 3804.5 18 4151.0 19 4434.0 20 4867.0 21 5575.5 22 6331.0 23 7537.5 24 8616.0 25 9837.5 26 12006.5 27 14049.5 28 16088.5 29 18427.5 30 21323.5 31 24099.0 32 26442.5 33 29712.0 34 32710.5 35 34032.5 36 36371.0 37 40407.0 38 41997.0 39 41975.0 40 42443.5 41 42579.0 42 42166.5 43 42282.0 44 43304.0 45 44248.5 46 43913.0 47 43374.0 48 43585.0 49 42460.5 50 41510.5 51 38651.0 52 34870.5 53 34340.5 54 32915.0 55 29316.0 56 26152.0 57 23561.5 58 21105.0 59 18712.0 60 16203.0 61 13420.5 62 11032.0 63 8452.0 64 5828.0 65 4204.5 66 3052.0 67 2361.0 68 1684.5 69 1105.0 70 871.0 71 711.0 72 601.5 73 609.0 74 485.0 75 310.0 76 204.5 77 142.0 78 99.0 79 39.5 80 13.5 81 11.5 82 4.5 83 2.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06505204929261636 2 2.2959546809158717E-4 3 0.0 4 0.0 5 0.0662000266330743 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 1.1479773404579358E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0026020819717046543 44-45 0.00803584138320555 46-47 0.009796073305241052 48-49 0.007614916358370975 50-51 0.0068113322200504195 52-53 0.005739886702289679 54-55 0.0063904071952158425 56-57 0.005089366209363516 58-59 0.003137804730585025 60-61 0.00214289103552148 62-63 0.0014541046312467186 64-65 0.0017984978333840996 66-67 0.0025638160603560565 68-69 0.001415838719898121 70-71 0.0027168797057504483 72-73 0.002487284237658861 74-75 8.418500496691529E-4 76-77 4.5919093618317433E-4 78-79 7.653182269719572E-5 80-81 3.4439320213738075E-4 82-83 3.826591134859786E-5 84-85 2.2959546809158717E-4 86-87 2.2959546809158717E-4 88-89 8.801159610177508E-4 90-91 0.0012627750745037294 92-93 4.2092502483457645E-4 94-95 8.801159610177508E-4 96-97 0.0012627750745037294 98-99 0.0017984978333840996 100-101 6.887864042747615E-4 102-103 9.566477837149465E-4 104-105 6.887864042747615E-4 106-107 3.4439320213738075E-4 108-109 8.035841383205551E-4 110-111 0.00107144551776074 112-113 0.010331796064121422 114-115 0.019515614787784907 116-117 0.017946712422492395 118-119 0.011250177936487771 120-121 0.005854684436335473 122-123 0.00608427990442706 124-125 0.0052041639434093085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1306646.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.32172739658234 #Duplication Level Percentage of deduplicated Percentage of total 1 43.857777103851205 11.544124531476417 2 14.546190531607293 7.657617236634286 3 10.9423275515555 8.640628886884684 4 8.306887268561951 8.746064887889125 5 6.00931140945555 7.908772838043049 6 4.255534096688087 6.720780505193106 7 2.933735838752239 5.40546965008542 8 2.0166430101412662 4.246522205532932 9 1.3881744903732305 3.288523546304441 >10 5.411772086628479 23.131263180834353 >50 0.2281822239975466 4.127235549105104 >100 0.09758573151506729 4.386081505562596 >500 0.0032332612799466404 0.5545752707153572 >1k 0.0017635970617890769 1.0625748839292293 >5k 0.0 0.0 >10k+ 8.817985308945384E-4 2.579765321809831 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12539 0.9596325248001372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10596 0.8109311932994859 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10209 0.7813133779156711 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3385 0.2590602198300075 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.2362537366662432 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2582 0.19760516620415933 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1791 0.13706849445067754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1564 0.11969577069841411 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1325 0.10140466507378434 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1479773404579358E-4 2.2959546809158717E-4 12-13 0.0 0.0 0.0 1.5306364539439143E-4 2.2959546809158717E-4 14-15 0.0 0.0 0.0 1.5306364539439143E-4 2.2959546809158717E-4 16-17 0.0 0.0 0.0 1.5306364539439143E-4 2.2959546809158717E-4 18-19 0.0 0.0 0.0 2.2959546809158717E-4 2.2959546809158717E-4 20-21 0.0 0.0 0.0 2.67861379440185E-4 2.2959546809158717E-4 22-23 0.0 0.0 0.0 3.8265911348597857E-4 2.2959546809158717E-4 24-25 0.0 0.0 0.0 0.0011479773404579358 2.2959546809158717E-4 26-27 0.0 0.0 0.0 0.001377572808549523 2.2959546809158717E-4 28-29 0.0 0.0 0.0 0.0018367637447326973 2.2959546809158717E-4 30-31 0.0 0.0 0.0 0.004859770741271929 2.2959546809158717E-4 32-33 0.0 0.0 0.0 0.014005323553586817 2.2959546809158717E-4 34-35 0.0 0.0 0.0 0.02410752414961665 2.2959546809158717E-4 36-37 0.0 0.0 0.0 0.04002614327063336 2.2959546809158717E-4 38-39 0.0 0.0 0.0 0.06887864042747616 2.2959546809158717E-4 40-41 0.0 0.0 0.0 0.0999888263538862 2.2959546809158717E-4 42-43 0.0 0.0 0.0 0.1380251422343925 2.2959546809158717E-4 44-45 0.0 0.0 0.0 0.18677591329250615 2.2959546809158717E-4 46-47 0.0 0.0 0.0 0.24272067568415623 2.2959546809158717E-4 48-49 0.0 0.0 0.0 0.30142058369290536 2.2959546809158717E-4 50-51 0.0 0.0 0.0 0.3588577166271507 2.2959546809158717E-4 52-53 0.0 0.0 0.0 0.4280424843454157 2.2959546809158717E-4 54-55 0.0 0.0 0.0 0.5064876026100413 2.2959546809158717E-4 56-57 0.0 0.0 0.0 0.6086575859107975 2.2959546809158717E-4 58-59 0.0 0.0 0.0 0.7326008727689061 2.2959546809158717E-4 60-61 0.0 0.0 0.0 0.8500389546977529 2.2959546809158717E-4 62-63 0.0 0.0 0.0 0.9695816617507726 2.2959546809158717E-4 64-65 0.0 0.0 0.0 1.1013311945239952 2.2959546809158717E-4 66-67 0.0 0.0 0.0 1.2723798182522275 2.2959546809158717E-4 68-69 0.0 0.0 0.0 1.4882760900810166 2.2959546809158717E-4 70-71 0.0 0.0 0.0 1.7099887804347926 2.2959546809158717E-4 72-73 0.0 0.0 0.0 1.9113440059511144 2.2959546809158717E-4 74-75 0.0 0.0 0.0 2.1355439805425496 2.2959546809158717E-4 76-77 0.0 0.0 0.0 2.4052421237274673 2.2959546809158717E-4 78-79 0.0 0.0 0.0 2.7491761349286645 2.2959546809158717E-4 80-81 0.0 0.0 0.0 3.087408525338921 2.2959546809158717E-4 82-83 0.0 0.0 0.0 3.420742879096557 2.2959546809158717E-4 84-85 0.0 0.0 0.0 3.759013535418162 2.2959546809158717E-4 86-87 0.0 0.0 0.0 4.128279579932132 2.2959546809158717E-4 88-89 0.0 0.0 0.0 4.5824194158172915 2.2959546809158717E-4 90-91 0.0 0.0 0.0 5.086343202366976 2.2959546809158717E-4 92-93 0.0 0.0 0.0 5.5690294081181895 2.2959546809158717E-4 94-95 0.0 0.0 0.0 6.0784634859020725 2.2959546809158717E-4 96-97 0.0 0.0 0.0 6.621341970204631 2.2959546809158717E-4 98-99 0.0 0.0 0.0 7.239833895332018 2.2959546809158717E-4 100-101 0.0 0.0 0.0 7.900800982056349 2.2959546809158717E-4 102-103 0.0 0.0 0.0 8.559012923163582 2.2959546809158717E-4 104-105 0.0 0.0 0.0 9.251281525371065 2.2959546809158717E-4 106-107 0.0 0.0 0.0 9.951777298518497 2.2959546809158717E-4 108-109 0.0 0.0 0.0 10.682503141631322 2.2959546809158717E-4 110-111 0.0 0.0 0.0 11.460525651171015 2.2959546809158717E-4 112-113 0.0 0.0 0.0 12.221902489274065 2.2959546809158717E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5970 0.0 77.43054 1 GTACATG 13200 0.0 50.904964 1 TACATGG 13520 0.0 49.275246 2 ACATGGG 13205 0.0 49.188297 3 GTATCAA 9520 0.0 48.403797 2 CATGGGA 9840 0.0 45.438107 4 CATGGGG 3325 0.0 43.331043 4 ATGGGAG 3530 0.0 43.00717 5 ATGGGAT 3975 0.0 40.888477 5 TACCTGG 1020 0.0 37.35556 2 CGGGAGT 385 0.0 37.112988 4 ATCAACG 13280 0.0 36.447514 4 TCAACGC 13455 0.0 35.973465 5 CAACGCA 13490 0.0 35.900894 6 TATCAAC 13730 0.0 35.469757 3 AACGCAG 13700 0.0 35.394016 7 GTCGGGA 410 0.0 33.39792 2 TGGGATA 1180 0.0 33.27781 6 AGTACAT 7860 0.0 32.948994 2 TATCACG 145 1.09228495E-7 32.84713 2 >>END_MODULE