FastQCFastQC Report
Thu 26 May 2016
SRR936249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805648
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25410.14072510256705625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG165650.055.345741
GGTATCA33800.055.0935331
TACATGG165850.053.8441122
ACATGGG168400.051.2976573
CATGGGA110050.047.5208134
CATGGGG55600.045.370764
GTATCAA42200.043.845092
ATGGGAT44700.041.7933465
ATGGGAG38350.041.2668465
TCAACGC49500.037.1396945
ATCAACG49900.036.841984
CAACGCA50100.036.8136636
ATGGGGG29650.036.319425
AACGCAG52650.034.8046537
AGTACAT91550.032.817542
TATCAAC58050.031.9760913
GAGTACA99000.031.7902181
TACCTGG14100.030.8018442
ATGGGAC22100.030.690055
GTCGGGA5450.030.5657142
Produced by FastQC (version 0.11.5)