##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936249_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1805648 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.483251442141547 31.0 30.0 34.0 25.0 34.0 2 29.436991041443292 31.0 30.0 34.0 23.0 34.0 3 29.681655006955953 31.0 30.0 34.0 25.0 34.0 4 33.05525938610405 35.0 35.0 37.0 26.0 37.0 5 33.368678723649346 37.0 35.0 37.0 28.0 37.0 6 33.228214469265325 36.0 35.0 37.0 27.0 37.0 7 33.21628911061292 37.0 35.0 37.0 27.0 37.0 8 33.08108667913126 37.0 35.0 37.0 26.0 37.0 9 34.396376813199474 39.0 35.0 39.0 25.0 39.0 10-11 34.29812593595208 39.0 35.0 39.0 25.0 39.0 12-13 34.28818573719795 39.0 35.0 39.0 25.0 39.0 14-15 35.388553582979625 39.0 35.5 41.0 25.0 41.0 16-17 35.42140910077711 39.0 35.5 41.0 25.0 41.0 18-19 35.45111477984635 39.0 35.0 41.0 25.0 41.0 20-21 35.57045725412705 39.0 35.0 41.0 26.0 41.0 22-23 35.467583105898825 39.0 35.0 41.0 25.5 41.0 24-25 35.442439500943706 39.0 34.0 41.0 26.5 41.0 26-27 35.53548227561518 39.0 35.0 41.0 27.0 41.0 28-29 35.42944112030695 39.0 34.0 41.0 27.0 41.0 30-31 35.34885564628322 39.0 34.0 41.0 26.5 41.0 32-33 35.18475970953364 39.0 34.0 41.0 25.5 41.0 34-35 35.135341162840156 39.0 34.0 41.0 25.5 41.0 36-37 35.02167615171949 39.0 34.0 41.0 25.0 41.0 38-39 34.77117550042976 39.0 34.0 40.0 24.5 41.0 40-41 34.86096321099129 38.5 33.5 40.5 25.0 41.0 42-43 34.776007837629486 39.0 34.0 41.0 24.0 41.0 44-45 34.648838810222145 38.5 33.0 40.0 24.0 41.0 46-47 34.61265207836743 38.0 33.0 40.0 24.0 41.0 48-49 34.51452359485348 38.0 33.0 40.0 24.0 41.0 50-51 34.373917840021974 38.0 33.0 40.0 24.0 41.0 52-53 34.22020404862963 38.0 33.0 40.0 23.5 41.0 54-55 34.04747436931229 38.0 33.0 40.0 23.0 41.0 56-57 33.84831816610989 38.0 33.0 40.0 23.0 41.0 58-59 33.61863054150089 37.5 32.0 40.0 22.0 41.0 60-61 33.37722939354735 37.0 32.0 40.0 21.5 41.0 62-63 33.12678523167306 37.0 32.0 40.0 20.5 41.0 64-65 32.83854632796647 36.0 31.0 39.5 19.5 41.0 66-67 32.527626647054134 36.0 31.0 39.0 18.5 41.0 68-69 32.2371846561456 35.5 31.0 39.0 18.0 40.5 70-71 31.898052112039554 35.0 31.0 39.0 14.5 40.0 72-73 31.571755679955338 35.0 31.0 38.0 13.0 40.0 74-75 31.217449912718315 35.0 30.0 37.0 10.5 39.5 76-77 30.884860172082266 35.0 30.0 37.0 7.5 39.0 78-79 30.555137546188405 34.5 30.0 36.0 7.0 39.0 80-81 30.24134770453599 34.0 30.0 36.0 6.0 37.5 82-83 29.934537905505394 34.0 30.0 35.5 2.0 37.0 84-85 29.960737641002012 34.0 30.0 35.0 2.0 37.0 86-87 29.840576900924212 34.0 30.0 35.0 2.0 36.5 88-89 29.670282081557424 34.0 30.0 35.0 2.0 36.0 90-91 29.50130202564398 34.0 30.0 35.0 2.0 36.0 92-93 29.370001794369664 34.0 30.0 35.0 2.0 36.0 94-95 29.240926803009224 34.0 30.0 35.0 2.0 35.0 96-97 29.134305523557195 34.0 30.0 35.0 2.0 35.0 98-99 29.014242255411908 34.0 29.0 35.0 2.0 35.0 100-101 28.897635641055178 34.0 29.0 35.0 2.0 35.0 102-103 27.905235405793377 32.5 27.5 34.5 2.0 35.0 104-105 28.567450300390774 34.0 29.0 35.0 2.0 35.0 106-107 28.687257150895412 34.0 29.0 35.0 2.0 35.0 108-109 28.623315009348445 34.0 29.0 35.0 2.0 35.0 110-111 28.488206449983608 34.0 29.0 35.0 2.0 35.0 112-113 28.42955271459332 34.0 29.0 35.0 2.0 35.0 114-115 28.311911568589228 34.0 29.0 35.0 2.0 35.0 116-117 28.1706985525418 34.0 29.0 35.0 2.0 35.0 118-119 27.954915354487696 34.0 29.0 35.0 2.0 35.0 120-121 27.736370543981995 34.0 28.5 35.0 2.0 35.0 122-123 27.4216525036995 34.0 27.5 35.0 2.0 35.0 124-125 25.41253666273825 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6881241123418356 1101 2 0.7558736894584221 1101 3 0.6744074047834268 1101 4 0.7866144153582226 1101 5 0.6860447595132513 1101 6 0.7124102953434388 1101 7 0.6565701793925172 1101 8 0.7097999940984607 1101 9 0.7901331191713652 1101 10-11 0.8638066947348264 1101 12-13 0.7734809474036197 1101 14-15 0.9341171346473018 1101 16-17 0.9587441700641506 1101 18-19 0.9655703018083486 1101 20-21 0.7661769042794191 1101 22-23 0.9293688998175398 1101 24-25 0.9448291536894757 1101 26-27 0.7772071425296048 1101 28-29 0.9731269726344394 1101 30-31 0.9888700692140091 1101 32-33 0.9258586022128625 1101 34-35 0.8056956852042063 1101 36-37 0.7875773443018161 1101 38-39 0.9599402222432261 1101 40-41 0.9666113791645472 1101 42-43 1.0647257597130206 1101 44-45 1.0176338081498528 1101 46-47 0.8716284370996306 1101 48-49 0.970970239640188 1101 50-51 0.9876962653440557 1101 52-53 0.9389565972213774 1101 54-55 0.9032738636550661 1101 56-57 0.930664590422424 1101 58-59 1.0053534159210145 1101 60-61 0.9733250927759514 1101 62-63 1.0252200076637479 1101 64-65 0.9830517880766791 1101 66-67 0.9812262910059815 1101 68-69 0.8571425355170348 1101 70-71 0.9101026918084933 1101 72-73 0.8993714579240759 1101 74-75 0.831365607288717 1101 76-77 0.8405282456239682 1101 78-79 0.8221589329884793 1101 80-81 0.9315552462973109 1101 82-83 0.927881483660201 1101 84-85 0.6235069857641555 1101 86-87 0.7953864048007588 1101 88-89 0.7264506776200648 1101 90-91 0.7460270577950787 1101 92-93 0.7432740298584584 1101 94-95 0.8765956733060918 1101 96-97 0.8636851821095668 1101 98-99 0.8530631143799994 1101 100-101 0.9192707501182937 1101 102-103 0.9930291305718804 1101 104-105 0.9512772612986744 1101 106-107 0.9684437599852309 1101 108-109 0.936966474057833 1101 110-111 1.0692879054147149 1101 112-113 1.0699332732811797 1101 114-115 1.1151030084472708 1101 116-117 1.1356476552680768 1101 118-119 1.0454484976202956 1101 120-121 1.1634098084552207 1101 122-123 1.149108807520136 1101 124-125 0.9737748549386076 1102 1 0.19799660981830414 1102 2 0.20057346307742208 1102 3 0.3531583281049855 1102 4 0.24498730053419138 1102 5 0.3824283967078941 1102 6 0.3099779664450466 1102 7 0.32336572989190415 1102 8 0.31055717017642337 1102 9 0.39021365512755324 1102 10-11 0.4784575561995368 1102 12-13 0.3584421891664604 1102 14-15 0.5299348255068566 1102 16-17 0.47560734142264494 1102 18-19 0.45070197353481944 1102 20-21 0.3390651502834743 1102 22-23 0.46331976178776557 1102 24-25 0.5445478819857925 1102 26-27 0.3790064035764473 1102 28-29 0.41482070346754796 1102 30-31 0.39057015217886715 1102 32-33 0.5599294543737727 1102 34-35 0.5450961509860974 1102 36-37 0.46721796061147813 1102 38-39 0.45676927308653603 1102 40-41 0.42696005842788054 1102 42-43 0.6213751976196349 1102 44-45 0.5380595119424996 1102 46-47 0.28926981857485856 1102 48-49 0.3413119120780621 1102 50-51 0.3061609827167331 1102 52-53 0.4277123253289403 1102 54-55 0.43543855845252466 1102 56-57 0.46056720134338036 1102 58-59 0.5035358086551227 1102 60-61 0.44621633483594536 1102 62-63 0.5189901223880327 1102 64-65 0.361204782474303 1102 66-67 0.3282354072767433 1102 68-69 0.3287599606276572 1102 70-71 0.25284563403159055 1102 72-73 0.17186718361259778 1102 74-75 0.23384293867334094 1102 76-77 0.3111528419115963 1102 78-79 0.32731110118977114 1102 80-81 0.2709014255184172 1102 82-83 0.20956264831961136 1102 84-85 0.29649272071578636 1102 86-87 0.35385739539671945 1102 88-89 0.3426671324277706 1102 90-91 0.3312540608156702 1102 92-93 0.30531599856064773 1102 94-95 0.3074339413983509 1102 96-97 0.3548480500526665 1102 98-99 0.37146728183215316 1102 100-101 0.41377428207369604 1102 102-103 0.44665135602989636 1102 104-105 0.5293558351464576 1102 106-107 0.3805505754206635 1102 108-109 0.4443833153197616 1102 110-111 0.48243655923006656 1102 112-113 0.46386426264665914 1102 114-115 0.43647747561475114 1102 116-117 0.43083375780987865 1102 118-119 0.44647046092958576 1102 120-121 0.4200242183992664 1102 122-123 0.4455920296685605 1102 124-125 0.3707615131193229 1103 1 0.18441244356770525 1103 2 0.31274174847679603 1103 3 0.29326835796754125 1103 4 0.31416575050734963 1103 5 0.24155625932129254 1103 6 0.22134881795183503 1103 7 0.18438002058552172 1103 8 0.20551068067541678 1103 9 0.31525110107821064 1103 10-11 0.3866890710884263 1103 12-13 0.33737118787587406 1103 14-15 0.4716474949556684 1103 16-17 0.337569556338849 1103 18-19 0.41482569118763735 1103 20-21 0.279142208868727 1103 22-23 0.3687098591001359 1103 24-25 0.4254615160799702 1103 26-27 0.251175583910495 1103 28-29 0.3600377486758717 1103 30-31 0.3054371366258408 1103 32-33 0.4510123724260211 1103 34-35 0.3939475729901858 1103 36-37 0.413175682013609 1103 38-39 0.4816072428094813 1103 40-41 0.3937933085479983 1103 42-43 0.5490750742128583 1103 44-45 0.6074781812217438 1103 46-47 0.4897811088822408 1103 48-49 0.30370110503539394 1103 50-51 0.3313922526761317 1103 52-53 0.3854054663612203 1103 54-55 0.30323315681526 1103 56-57 0.363098217894958 1103 58-59 0.30882510806258523 1103 60-61 0.23978199006942447 1103 62-63 0.2440238001927142 1103 64-65 0.20365977668078017 1103 66-67 0.33369921894480115 1103 68-69 0.3094552777642434 1103 70-71 0.30526760985893375 1103 72-73 0.30508291939019827 1103 74-75 0.23195651059311828 1103 76-77 0.1912798755328815 1103 78-79 0.20235584792325767 1103 80-81 0.24733402751741806 1103 82-83 0.11255644029911238 1103 84-85 0.2220011849136867 1103 86-87 0.20601772058771672 1103 88-89 0.19399728795799476 1103 90-91 0.17972749067634197 1103 92-93 0.17325543503441665 1103 94-95 0.32814579127541776 1103 96-97 0.33373112448296993 1103 98-99 0.27266767183525786 1103 100-101 0.3234530005272447 1103 102-103 0.33492582778293567 1103 104-105 0.4237158626539248 1103 106-107 0.41819553128625486 1103 108-109 0.3206056076713679 1103 110-111 0.271200183432871 1103 112-113 0.2760539793762895 1103 114-115 0.24914292077409783 1103 116-117 0.2550226643881217 1103 118-119 0.2211836108170111 1103 120-121 0.21674922852615808 1103 122-123 0.27696514206293443 1103 124-125 0.17069180241873383 1104 1 0.0996349342287921 1104 2 0.2678477316529566 1104 3 0.19546805139530576 1104 4 0.2678655656106699 1104 5 0.22693160960891845 1104 6 0.16548305243694728 1104 7 0.23010080402560362 1104 8 0.2916810615826435 1104 9 0.3423717053917983 1104 10-11 0.34449424468307654 1104 12-13 0.3559788933256911 1104 14-15 0.37267602975613556 1104 16-17 0.39275814091340067 1104 18-19 0.3382816659593999 1104 20-21 0.2984010667240753 1104 22-23 0.34924158129852145 1104 24-25 0.27773201966626715 1104 26-27 0.2551402628751731 1104 28-29 0.3753504924973967 1104 30-31 0.31188023385841745 1104 32-33 0.40719054307787417 1104 34-35 0.34112626797590195 1104 36-37 0.38652007904221364 1104 38-39 0.5885722905640094 1104 40-41 0.2852972184729623 1104 42-43 0.4532049559743143 1104 44-45 0.39473812286765764 1104 46-47 0.23799028296861025 1104 48-49 0.3823255941861987 1104 50-51 0.461446178782694 1104 52-53 0.5011126522438474 1104 54-55 0.4736210886066985 1104 56-57 0.3799996431297217 1104 58-59 0.4108231587448401 1104 60-61 0.5132495052036106 1104 62-63 0.47047079856953644 1104 64-65 0.4738502052045419 1104 66-67 0.5021849979569026 1104 68-69 0.35423301289285547 1104 70-71 0.5046322627323612 1104 72-73 0.5301795699254441 1104 74-75 0.5117020456018118 1104 76-77 0.6025151082593396 1104 78-79 0.5948241934617791 1104 80-81 0.5097502030914889 1104 82-83 0.5637439686796242 1104 84-85 0.5356709950747245 1104 86-87 0.6513285075651716 1104 88-89 0.593624609515139 1104 90-91 0.6247213567228407 1104 92-93 0.44932078609976855 1104 94-95 0.592137790793732 1104 96-97 0.5275734003427921 1104 98-99 0.5375798898176534 1104 100-101 0.566208224422823 1104 102-103 0.6527632140413715 1104 104-105 0.614769823883325 1104 106-107 0.6881353332435971 1104 108-109 0.6660941413177959 1104 110-111 0.5719635407751724 1104 112-113 0.6570375318510671 1104 114-115 0.6239481144389423 1104 116-117 0.7150136704317589 1104 118-119 0.7739884833587247 1104 120-121 0.7044345328781283 1104 122-123 0.71681589507158 1104 124-125 0.6995045750911508 1105 1 0.22144398515320773 1105 2 0.4070921288088911 1105 3 0.3410059220129753 1105 4 0.3000660254673093 1105 5 0.2670630282401092 1105 6 0.3593298762655337 1105 7 0.3038450648651718 1105 8 0.3041221620538366 1105 9 0.25250700843635343 1105 10-11 0.3552000257034891 1105 12-13 0.3894189877096892 1105 14-15 0.3759563685927887 1105 16-17 0.39847450153172304 1105 18-19 0.2330874694177396 1105 20-21 0.1975843748449151 1105 22-23 0.030146432472413665 1105 24-25 0.3830825578143404 1105 26-27 0.16108703434862548 1105 28-29 0.36462649574968253 1105 30-31 0.39839812266768604 1105 32-33 0.4815347768954297 1105 34-35 0.5819966902188725 1105 36-37 0.48806286669991295 1105 38-39 0.43628579457026007 1105 40-41 0.3054884474193216 1105 42-43 0.5629232877514667 1105 44-45 0.621742094137467 1105 46-47 0.48334080905328847 1105 48-49 0.42782154450431165 1105 50-51 0.35537997969323953 1105 52-53 0.37599081194872497 1105 54-55 0.3117098727853147 1105 56-57 0.3034797759235772 1105 58-59 0.4194972226856706 1105 60-61 0.4554990751435568 1105 62-63 0.3739820307971371 1105 64-65 0.2566970821120691 1105 66-67 0.456604181651052 1105 68-69 0.4239771723998942 1105 70-71 0.31796261640699086 1105 72-73 0.30588123732798067 1105 74-75 0.3084568715632017 1105 76-77 0.3639797371772282 1105 78-79 0.3654892725855916 1105 80-81 0.43772069361327937 1105 82-83 0.3549293661805706 1105 84-85 0.2776640369081669 1105 86-87 0.32532392830021806 1105 88-89 0.3175500022389812 1105 90-91 0.3802412649445266 1105 92-93 0.4237129719146644 1105 94-95 0.4489710408896386 1105 96-97 0.34230781262622045 1105 98-99 0.39834315291789935 1105 100-101 0.4502245268955747 1105 102-103 0.4794379149294805 1105 104-105 0.4028905157431879 1105 106-107 0.45656295407659897 1105 108-109 0.38339956477916814 1105 110-111 0.5260161178783669 1105 112-113 0.38945428839003426 1105 114-115 0.3979287575144319 1105 116-117 0.4192385128234939 1105 118-119 0.4585535496953348 1105 120-121 0.4984856503104602 1105 122-123 0.4527429790834816 1105 124-125 0.47803034371040454 1106 1 -0.3979472834831448 1106 2 -0.4122653441284072 1106 3 -0.3175334573439841 1106 4 -0.2903184564603123 1106 5 -0.26417565305266066 1106 6 -0.1611336334106852 1106 7 -0.37500880937680137 1106 8 -0.4801203437142476 1106 9 -0.37454651995820853 1106 10-11 -0.36776864363957884 1106 12-13 -0.3585349702847722 1106 14-15 -0.6345486988607618 1106 16-17 -0.4419068907750301 1106 18-19 -0.4774251557237932 1106 20-21 -0.5722034585133002 1106 22-23 -0.6023027622773256 1106 24-25 -4.279295200147608 1106 26-27 -1.3264472960925104 1106 28-29 -0.9139044893450361 1106 30-31 -0.8082579426383418 1106 32-33 -5.817675159515417 1106 34-35 -1.5058604818368835 1106 36-37 -0.7346327276908582 1106 38-39 -0.3576949585176976 1106 40-41 -0.8829720087608308 1106 42-43 -0.4580216086226443 1106 44-45 -0.41790617866939783 1106 46-47 -0.41142717943240825 1106 48-49 -0.34062574989092553 1106 50-51 -0.4070600222460996 1106 52-53 -0.30051815888430156 1106 54-55 -0.4275916019620283 1106 56-57 -0.42482930309371625 1106 58-59 -0.2708217592795066 1106 60-61 -0.22818328236075303 1106 62-63 -0.23580824098492315 1106 64-65 -0.2068331519671176 1106 66-67 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-1.462183803539908 2216 118-119 -1.541321296467185 2216 120-121 -1.6732294598396287 2216 122-123 -1.6051759124040998 2216 124-125 -1.6288648849987375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53508.0 3 9674.0 4 7232.0 5 6035.0 6 6513.0 7 6240.0 8 6157.0 9 6787.0 10 7894.0 11 9258.0 12 9979.0 13 10545.0 14 10562.0 15 10090.0 16 9498.0 17 8422.0 18 7599.0 19 6938.0 20 6944.0 21 7021.0 22 8380.0 23 11524.0 24 17458.0 25 24366.0 26 30145.0 27 28378.0 28 31718.0 29 39540.0 30 46196.0 31 55616.0 32 71765.0 33 93797.0 34 127590.0 35 181833.0 36 294927.0 37 400495.0 38 134567.0 39 457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.46368523567505 17.959696351936678 9.33495713161461 22.241661280773663 2 18.897945665983254 21.652306155248592 36.74934067918075 22.700407499587406 3 17.159297647101173 29.762273315075777 29.58300219589122 23.49542684193183 4 10.86572580042212 16.587184274171886 36.21340011663448 36.33368980877151 5 10.821086467231417 43.83667325346472 33.89826941480877 11.44397086449509 6 30.290883172106938 37.31930911992929 19.13416801475821 13.255639693205563 7 28.220091358593557 35.173003136851804 21.099814358629 15.507091145925637 8 27.22106534043708 34.89012084491753 19.387966460274033 18.500847354371352 9 27.904745975607188 14.663322282614114 18.199183214131036 39.23274852764766 10-11 23.991064545285376 28.09553637096703 28.41528874356452 19.49811034018307 12-13 24.311476935141922 27.172264793387697 27.853034220128002 20.663224051342375 14-15 22.460860482323803 28.20122153842064 24.92725377604686 24.410664203208697 16-17 22.37521533694746 30.546513472941548 25.442255914306628 21.636015275804365 18-19 21.772442275388944 29.365185736146287 26.975507850857248 21.88686413760752 20-21 23.276991701336737 28.393706883947207 26.624037988648595 21.70526342606746 22-23 22.974295812121966 28.510158176206605 26.644481570498606 21.87106444117282 24-25 22.561538896082535 28.51239417680269 26.88468580631655 22.041381120798224 26-27 22.25023662245468 28.647544138602882 26.761930396168847 22.340288842773585 28-29 22.255417942081976 28.578339529724122 26.840571747891723 22.32567078030218 30-31 22.407987487981995 28.30065949500007 26.990004563519314 22.301348453498623 32-33 22.076097680926733 28.676984304780618 26.79726309091065 22.449654923382 34-35 22.574306557053834 28.45619363456925 26.878293867839886 22.09120594053703 36-37 22.606977600677876 28.223505858049936 26.780190902369526 22.38932563890266 38-39 22.298751459326063 28.481420314704913 26.83564942501999 22.384178800949034 40-41 22.220280756281905 28.405486638129883 27.05697701015107 22.317255595437143 42-43 21.986218011289765 28.256704423832847 27.24513912830375 22.511938436573637 44-45 22.169799434376642 28.296121245988566 27.074379222410492 22.459700097224303 46-47 22.387617051725396 28.446753787635437 26.772889192140948 22.392739968498216 48-49 22.211044283467356 28.2217939345606 26.963901995968186 22.603259786003854 50-51 22.16687042834258 28.36365297840495 27.097441464093407 22.372035129159055 52-53 22.377096437074627 28.099636524346444 27.142044601780107 22.381222436798822 54-55 22.161147462131417 28.328919797332713 26.996745438338476 22.51318730219739 56-57 22.290428924096815 28.583494058143383 26.849216821345546 22.276860196414255 58-59 22.447837472838437 28.001991548104666 26.82018829433818 22.729982684718717 60-61 22.44031169176409 28.24869989975798 27.164258458266644 22.146729950211284 62-63 21.96507798139479 28.353759339574207 27.14337378289931 22.537788896131694 64-65 22.027420429884508 28.471949732627134 26.899059023623128 22.60157081386523 66-67 21.974406325951193 28.66917380886516 26.873966953019192 22.482452912164458 68-69 22.048101424257176 28.387034424855855 27.040035045687326 22.524829105199643 70-71 21.95742500917266 28.370146277976616 27.132467064956284 22.53996164789444 72-73 22.081063022095435 28.416640152588172 26.696174136160245 22.806122689156144 74-75 21.911615296621893 28.880444363094426 26.682498537222664 22.52544180306102 76-77 21.685170267930005 28.66740564769435 26.968866155176602 22.678557929199044 78-79 22.013370856428747 28.525924414204685 26.95914226700896 22.5015624623576 80-81 22.02691124859399 28.667967406552474 26.76645220888561 22.538669135967933 82-83 22.051030560573004 28.45160188043764 26.794436443295698 22.702931115693655 84-85 21.816407580465885 28.353587451769208 27.086170506761864 22.743834461003047 86-87 21.710855510828427 28.516833777266292 27.195252875641636 22.57705783626364 88-89 21.964436927245064 28.363259060375924 27.09168564888735 22.580618363491663 90-91 21.936349438426266 28.45439844044217 27.015767262577256 22.59348485855431 92-93 21.901096147216297 28.29372370222788 27.0757085081903 22.729471642365528 94-95 21.70275406580328 28.370557910369154 27.11825920954875 22.80842881427882 96-97 22.03066173839939 28.44822716402734 26.991789051847604 22.529322045725667 98-99 21.881249532712868 28.661883492510942 26.7291839159077 22.727683058868486 100-101 21.624550885767494 28.595357595309135 27.047787831167664 22.73230368775571 102-103 21.716904035604234 28.23712857797952 27.04831781518674 22.997649571229513 104-105 21.522438209462425 28.386915085068342 27.218712071892654 22.871934633576583 106-107 21.495621352569383 28.549279686537304 27.187544565285116 22.7675543956082 108-109 21.653200002326056 28.92741403769263 26.570122419773913 22.849263540207403 110-111 21.51370184306556 28.890533126056074 26.821812627482995 22.773952403395377 112-113 21.521700362974194 28.775895279120178 26.99754878884915 22.704855569056477 114-115 21.374107102309544 29.01979798830109 26.571909762643454 23.034185146745916 116-117 21.37525216233093 28.894639413058858 26.990015963935075 22.740092460675136 118-119 21.480785302358242 28.888616283687124 26.78211343120906 22.848484982745568 120-121 21.30054150000069 28.977020517741014 26.794571985163095 22.9278659970952 122-123 21.442003595967844 28.812519262629987 26.774647235997783 22.97082990540439 124-125 21.69088062453237 28.79545689919192 26.43252610294749 23.081136373328224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1888.0 1 2222.0 2 2535.0 3 2179.5 4 1618.0 5 1232.5 6 1044.5 7 944.0 8 888.0 9 843.0 10 764.0 11 751.5 12 755.0 13 787.5 14 820.0 15 863.0 16 997.5 17 1143.5 18 1347.5 19 1565.0 20 2019.0 21 2800.5 22 3842.0 23 5225.5 24 6899.0 25 8882.0 26 11471.5 27 13984.0 28 16161.0 29 19249.0 30 23869.0 31 28349.5 32 32384.5 33 36324.0 34 40238.0 35 43139.5 36 46727.5 37 49884.0 38 51084.5 39 52996.5 40 53712.5 41 55055.5 42 56057.5 43 58008.0 44 63520.0 45 67276.0 46 68203.0 47 67875.5 48 65612.5 49 62594.0 50 62981.0 51 62081.5 52 58991.5 53 58004.0 54 56670.0 55 52966.5 56 47595.0 57 43505.0 58 40880.5 59 37554.0 60 31552.0 61 25706.5 62 21424.5 63 16394.0 64 12340.5 65 9799.0 66 7249.0 67 5633.0 68 4428.5 69 3053.5 70 2230.0 71 1647.0 72 1320.0 73 1115.5 74 833.5 75 673.5 76 464.0 77 276.0 78 179.0 79 123.0 80 90.0 81 69.5 82 48.0 83 24.0 84 13.5 85 8.5 86 4.5 87 2.0 88 2.5 89 2.5 90 2.0 91 3.0 92 2.5 93 1.0 94 1.5 95 2.5 96 2.0 97 1.5 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.769088991874385E-4 2 1.1076355967497541E-4 3 1.661453395124631E-4 4 3.876724588624139E-4 5 8.861084773998033E-4 6 4.984360185373894E-4 7 8.861084773998033E-4 8 0.0015506898354496557 9 0.0018829805144745819 10-11 0.0017722169547996066 12-13 0.002436798312849459 14-15 0.0031013796708993114 16-17 0.002630634542280666 18-19 0.0027137072120368977 20-21 0.0031013796708993114 22-23 0.08772473926258052 24-25 0.0023260347531744835 26-27 0.0016060716152871432 28-29 0.001495308055612168 30-31 0.0013291627160997048 32-33 0.0019106714043933258 34-35 0.001107635596749754 36-37 7.199631378873401E-4 38-39 0.001107635596749754 40-41 0.0013845444959371927 42-43 0.001689144285043375 44-45 0.0016337625052058875 46-47 0.0019937440741495576 48-49 4.4305423869990166E-4 50-51 0.0014122353858559366 52-53 0.0013845444959371927 54-55 0.0015783807253683996 56-57 0.0014122353858559366 58-59 9.691811471560348E-4 60-61 9.968720370747788E-4 62-63 0.0013845444959371927 64-65 0.0013014718261809611 66-67 3.5998156894367006E-4 68-69 2.769088991874385E-4 70-71 5.815086882936209E-4 72-73 1.9383622943120696E-4 74-75 4.1536334878115785E-4 76-77 4.7074512861864547E-4 78-79 2.492180092686947E-4 80-81 4.984360185373894E-4 82-83 2.492180092686947E-4 84-85 3.876724588624139E-4 86-87 4.1536334878115785E-4 88-89 6.091995782123648E-4 90-91 4.4305423869990166E-4 92-93 3.045997891061824E-4 94-95 4.7074512861864547E-4 96-97 5.53817798374877E-4 98-99 7.199631378873401E-4 100-101 5.815086882936209E-4 102-103 0.001107635596749754 104-105 6.368904681311086E-4 106-107 5.815086882936209E-4 108-109 9.137993673185471E-4 110-111 8.307266975623157E-4 112-113 5.53817798374877E-4 114-115 5.53817798374877E-4 116-117 8.584175874810595E-4 118-119 0.0013845444959371927 120-121 8.584175874810595E-4 122-123 8.030358076435716E-4 124-125 4.984360185373894E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1805648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.114786855375346 #Duplication Level Percentage of deduplicated Percentage of total 1 51.37524403984941 14.957792798631084 2 13.319341309566596 7.755795665640547 3 9.658808970289003 8.436424933402552 4 7.022344459966296 8.178162487077783 5 4.993120106483115 7.268681382177247 6 3.400168312459743 5.939710541380001 7 2.321304792422719 4.730900598042414 8 1.6229402936397777 3.7801248582657916 9 1.141615701493956 2.9914108037771703 >10 4.759941947568104 23.08038964315709 >50 0.24692406050509558 4.961067118738021 >100 0.13305478532008524 6.870338819431246 >500 0.0049989530125549705 0.9069596156016259 >1k 1.9226742355980654E-4 0.14224073467743062 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2541 0.14072510256705625 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7690889918743854E-5 0.0 16-17 0.0 0.0 0.0 5.538177983748771E-5 0.0 18-19 0.0 0.0 0.0 8.307266975623156E-5 0.0 20-21 0.0 0.0 0.0 1.3845444959371928E-4 0.0 22-23 0.0 0.0 0.0 2.2152711934995083E-4 0.0 24-25 0.0 0.0 0.0 4.707451286186455E-4 0.0 26-27 0.0 0.0 0.0 6.922722479685962E-4 0.0 28-29 0.0 0.0 0.0 0.0010799447068310102 0.0 30-31 0.0 0.0 0.0 0.002796779881793129 0.0 32-33 0.0 0.0 0.0 0.008362648755460644 0.0 34-35 0.0 0.0 0.0 0.01536844390490284 0.0 36-37 0.0 0.0 0.0 0.028438543946549936 0.0 38-39 0.0 0.0 0.0 0.05128352812951362 0.0 40-41 0.0 0.0 0.0 0.07861443647931379 0.0 42-43 0.0 0.0 0.0 0.11079125056489415 0.0 44-45 0.0 0.0 0.0 0.14748167970722975 0.0 46-47 0.0 0.0 0.0 0.19419621100015064 0.0 48-49 0.0 0.0 0.0 0.2427937228075461 0.0 50-51 0.0 0.0 0.0 0.2927757791108787 0.0 52-53 0.0 0.0 0.0 0.3507605026007284 0.0 54-55 0.0 0.0 0.0 0.42012618184718176 0.0 56-57 0.0 0.0 0.0 0.5067155946230938 0.0 58-59 0.0 0.0 0.0 0.6094487962216335 0.0 60-61 0.0 0.0 0.0 0.7159202679592036 0.0 62-63 0.0 0.0 0.0 0.8227794121556361 0.0 64-65 0.0 0.0 0.0 0.9419056205860721 0.0 66-67 0.0 0.0 0.0 1.08767046511834 0.0 68-69 0.0 0.0 0.0 1.2651967603874066 0.0 70-71 5.538177983748771E-5 0.0 0.0 1.45161183132039 0.0 72-73 5.538177983748771E-5 0.0 0.0 1.635202431481662 0.0 74-75 5.538177983748771E-5 0.0 0.0 1.8339676393184055 0.0 76-77 5.538177983748771E-5 0.0 0.0 2.0708632025732587 0.0 78-79 5.538177983748771E-5 0.0 0.0 2.361119110701532 0.0 80-81 2.2152711934995083E-4 0.0 0.0 2.6601253400441283 0.0 82-83 2.2152711934995083E-4 0.0 0.0 2.9633405846543734 0.0 84-85 2.2152711934995083E-4 0.0 0.0 3.2746969509007293 0.0 86-87 2.2152711934995083E-4 0.0 0.0 3.607596829503868 0.0 88-89 2.2152711934995083E-4 0.0 0.0 4.005625681195892 0.0 90-91 2.2152711934995083E-4 0.0 0.0 4.431179277467147 0.0 92-93 2.2152711934995083E-4 0.0 0.0 4.868529192843788 0.0 94-95 2.2152711934995083E-4 0.0 0.0 5.32434893179623 0.0 96-97 2.2152711934995083E-4 0.0 0.0 5.81749599035914 0.0 98-99 2.2152711934995083E-4 0.0 0.0 6.356222253728301 0.0 100-101 2.2152711934995083E-4 0.0 0.0 6.947948880401939 0.0 102-103 2.2152711934995083E-4 0.0 0.0 7.524362444950511 0.0 104-105 2.2152711934995083E-4 0.0 0.0 8.128771499206934 0.0 106-107 2.2152711934995083E-4 0.0 0.0 8.761203734061123 0.0 108-109 2.2152711934995083E-4 0.0 0.0 9.431683251663669 0.0 110-111 2.2152711934995083E-4 0.0 0.0 10.130518240543008 0.0 112-113 2.2152711934995083E-4 0.0 0.0 10.839903458481388 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16565 0.0 55.34574 1 GGTATCA 3380 0.0 55.093533 1 TACATGG 16585 0.0 53.844112 2 ACATGGG 16840 0.0 51.297657 3 CATGGGA 11005 0.0 47.520813 4 CATGGGG 5560 0.0 45.37076 4 GTATCAA 4220 0.0 43.84509 2 ATGGGAT 4470 0.0 41.793346 5 ATGGGAG 3835 0.0 41.266846 5 TCAACGC 4950 0.0 37.139694 5 ATCAACG 4990 0.0 36.84198 4 CAACGCA 5010 0.0 36.813663 6 ATGGGGG 2965 0.0 36.31942 5 AACGCAG 5265 0.0 34.804653 7 AGTACAT 9155 0.0 32.81754 2 TATCAAC 5805 0.0 31.976091 3 GAGTACA 9900 0.0 31.790218 1 TACCTGG 1410 0.0 30.801844 2 ATGGGAC 2210 0.0 30.69005 5 GTCGGGA 545 0.0 30.565714 2 >>END_MODULE