FastQCFastQC Report
Thu 26 May 2016
SRR936249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805648
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109100.6042152180269909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88140.4881350074876166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78910.43701762469761546No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.12549511311174716No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.12067689826588571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19390.10738527110488866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61950.077.041621
GTATCAA90100.052.864282
GTACATG145400.046.1842351
TACATGG147400.045.279882
ACATGGG148200.043.869183
CATGGGA98800.041.5190474
ATGGGAT38850.039.844415
TCAACGC132100.037.2724385
ATCAACG133100.037.037134
CAACGCA133400.036.972866
ATGGGAG35300.036.2617575
AACGCAG136550.035.9456677
TATCAAC137650.035.899383
CATGGGG52100.034.8535654
CGTCTAG750.003883953431.778471
ACGCAGA157250.031.2138716
CGCAGAG159050.030.8606177
AGTACAT94000.030.085892
GAGTACA101350.028.9839381
CAGAGTA173200.028.201989
Produced by FastQC (version 0.11.5)