##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936249_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1805648 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50650237477072 31.0 31.0 34.0 30.0 34.0 2 31.321866166606117 31.0 31.0 34.0 28.0 34.0 3 31.73099795751996 31.0 31.0 34.0 30.0 34.0 4 35.594127426829594 37.0 35.0 37.0 33.0 37.0 5 35.189727455185064 37.0 35.0 37.0 33.0 37.0 6 35.406691669694204 37.0 35.0 37.0 33.0 37.0 7 35.239150155512036 37.0 35.0 37.0 32.0 37.0 8 35.325654280347 37.0 35.0 37.0 32.0 37.0 9 36.98977043144622 39.0 37.0 39.0 33.0 39.0 10-11 36.88841651307453 39.0 37.0 39.0 32.0 39.0 12-13 36.81670513854306 39.0 37.0 39.0 32.0 39.0 14-15 37.94982355364944 40.0 37.0 41.0 32.5 41.0 16-17 37.85885621117737 40.0 37.0 41.0 32.0 41.0 18-19 37.990970277706396 40.0 37.0 41.0 33.0 41.0 20-21 38.04502538700788 40.0 37.0 41.0 33.0 41.0 22-23 37.94673103506331 40.0 37.0 41.0 32.5 41.0 24-25 37.91195127732537 40.0 37.0 41.0 32.5 41.0 26-27 37.72699219338431 40.0 37.0 41.0 32.0 41.0 28-29 37.56979516494909 40.0 36.5 41.0 32.0 41.0 30-31 37.3664119474006 39.5 36.0 41.0 32.0 41.0 32-33 37.13168901136878 39.0 35.5 41.0 31.0 41.0 34-35 36.90854723622766 39.0 35.0 41.0 31.0 41.0 36-37 36.691626773324586 39.0 35.0 41.0 30.0 41.0 38-39 36.66100978706813 39.0 35.0 41.0 30.5 41.0 40-41 36.67076168777082 39.0 35.0 41.0 30.0 41.0 42-43 36.54067044075036 39.0 35.0 41.0 30.0 41.0 44-45 36.34614664652247 39.0 35.0 41.0 30.0 41.0 46-47 35.998354053503235 39.0 35.0 41.0 29.0 41.0 48-49 35.68310157904531 39.0 35.0 41.0 27.5 41.0 50-51 35.49330655808885 39.0 35.0 41.0 27.0 41.0 52-53 35.34659745421034 39.0 35.0 41.0 27.0 41.0 54-55 35.1681789584681 39.0 35.0 41.0 26.0 41.0 56-57 34.96432554960879 38.0 34.0 40.0 26.0 41.0 58-59 34.74884556679929 38.0 34.0 40.0 26.0 41.0 60-61 34.51951958521262 38.0 34.0 40.0 26.0 41.0 62-63 34.298951401380556 37.0 34.0 40.0 25.5 41.0 64-65 34.04765436009676 37.0 34.0 40.0 25.0 41.0 66-67 33.75269598504249 36.5 33.0 39.5 24.5 41.0 68-69 33.44636330004519 36.0 33.0 39.0 23.5 41.0 70-71 33.11778236954268 35.5 33.0 39.0 23.0 41.0 72-73 32.85412660717925 35.0 33.0 38.5 23.5 40.0 74-75 32.670603572789375 35.0 33.0 37.5 24.0 40.0 76-77 32.366394502139954 35.0 33.0 37.0 23.0 39.0 78-79 32.046727269102284 35.0 32.0 36.5 22.0 39.0 80-81 31.768154978157426 35.0 32.0 36.0 22.0 38.5 82-83 31.510760126004627 35.0 32.0 36.0 21.5 37.0 84-85 31.25924875723286 35.0 32.0 35.0 20.5 37.0 86-87 31.055543771543512 35.0 32.0 35.0 20.0 36.5 88-89 30.904534826278432 35.0 32.0 35.0 20.0 36.0 90-91 30.76937060822486 35.0 32.0 35.0 20.0 36.0 92-93 30.6312016517062 35.0 32.0 35.0 19.0 36.0 94-95 30.517736014992956 34.5 32.0 35.0 18.5 35.0 96-97 30.4488828941189 34.0 32.0 35.0 18.0 35.0 98-99 30.35759710641277 34.0 31.0 35.0 18.0 35.0 100-101 30.270067034106315 34.0 31.0 35.0 17.0 35.0 102-103 29.246340648897238 33.5 29.5 34.5 12.0 35.0 104-105 29.997838172224043 34.0 31.0 35.0 15.0 35.0 106-107 30.148543625335613 34.0 31.0 35.0 13.0 35.0 108-109 30.11481778286798 34.0 31.0 35.0 10.0 35.0 110-111 30.07468980665113 34.0 31.0 35.0 8.5 35.0 112-113 29.987498393928384 34.0 31.0 35.0 7.0 35.0 114-115 29.963956430046167 34.0 31.0 35.0 2.0 35.0 116-117 29.860237986584316 34.0 31.0 35.0 2.0 35.0 118-119 29.726954256865127 34.0 31.0 35.0 2.0 35.0 120-121 29.529413540180588 34.0 31.0 35.0 2.0 35.0 122-123 29.29467066670802 34.0 31.0 35.0 2.0 35.0 124-125 27.225180655365833 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20317475609057567 1101 2 0.6421309518653544 1101 3 0.44273120863040205 1101 4 0.17288404879792552 1101 5 0.4862327621808049 1101 6 0.37769431905778816 1101 7 0.4767022470632156 1101 8 0.39900800904243994 1101 9 0.4291617749129415 1101 10-11 0.4057232608490935 1101 12-13 0.3894036283507205 1101 14-15 0.51822004603099 1101 16-17 0.5956650152222309 1101 18-19 0.46127799868146013 1101 20-21 0.4781126151513604 1101 22-23 0.33322255440061355 1101 24-25 0.37411305273599993 1101 26-27 0.44349873117738525 1101 28-29 0.4099932278738976 1101 30-31 0.4462360400646119 1101 32-33 0.4200697840193044 1101 34-35 0.3116046104366319 1101 36-37 0.45528933987382914 1101 38-39 0.47982005070279854 1101 40-41 0.4935257877293324 1101 42-43 0.48860352227507775 1101 44-45 0.4172356021182466 1101 46-47 0.4167038326396124 1101 48-49 0.4772837557419223 1101 50-51 0.48016979444014396 1101 52-53 0.4691842970009219 1101 54-55 0.4591683706547016 1101 56-57 0.3010750965212168 1101 58-59 0.44430114722084824 1101 60-61 0.5070252837809619 1101 62-63 0.4121733913345764 1101 64-65 0.3984849816380702 1101 66-67 0.4489714982123516 1101 68-69 0.44962552630260433 1101 70-71 0.3491935799143633 1101 72-73 0.44701551506273063 1101 74-75 0.5005323305597642 1101 76-77 0.3951366167862034 1101 78-79 0.4197862980034657 1101 80-81 0.4630218740275289 1101 82-83 0.38320167218370216 1101 84-85 0.2870325593949872 1101 86-87 0.32492125340262135 1101 88-89 0.3484477317900918 1101 90-91 0.3460547750792351 1101 92-93 0.32405329065856137 1101 94-95 0.3100178988857536 1101 96-97 0.3436129169386142 1101 98-99 0.3520217240409238 1101 100-101 0.29456389508496983 1101 102-103 0.292189504700076 1101 104-105 0.2728142560729374 1101 106-107 0.23153048035253931 1101 108-109 0.2699828513235545 1101 110-111 0.3778248611236137 1101 112-113 0.3166399030777498 1101 114-115 0.2489417863473946 1101 116-117 0.31086352254172667 1101 118-119 0.39169211330694864 1101 120-121 0.44334322064370824 1101 122-123 0.2858933187655843 1101 124-125 0.286751517523836 1102 1 0.22254345628191885 1102 2 0.6217339756872029 1102 3 0.39855380907625815 1102 4 0.25657264490899934 1102 5 0.49577661821329144 1102 6 0.3772209915382021 1102 7 0.4754441501604134 1102 8 0.47290800957260615 1102 9 0.47286338105783443 1102 10-11 0.5415281882637899 1102 12-13 0.41474952177522084 1102 14-15 0.5789021998820374 1102 16-17 0.5289655974311103 1102 18-19 0.4651339719847485 1102 20-21 0.4480342479451309 1102 22-23 0.4998314370529968 1102 24-25 0.29852644532794415 1102 26-27 0.333262675259391 1102 28-29 0.4425935220791928 1102 30-31 0.36990553539440185 1102 32-33 0.3794605267982831 1102 34-35 0.4315025152071783 1102 36-37 0.40907080015642805 1102 38-39 0.3493378077591629 1102 40-41 0.3074871124693672 1102 42-43 0.4446045298375836 1102 44-45 0.5230840389229812 1102 46-47 0.4641610503787774 1102 48-49 0.3795541989843514 1102 50-51 0.4122701665058983 1102 52-53 0.45111222323827604 1102 54-55 0.4037416441343211 1102 56-57 0.43142325671477977 1102 58-59 0.45392508301992507 1102 60-61 0.5186620020122561 1102 62-63 0.49447671228380585 1102 64-65 0.48884097349718303 1102 66-67 0.40392949609735496 1102 68-69 0.47387385224094913 1102 70-71 0.3923299425027338 1102 72-73 0.48196080868422797 1102 74-75 0.4075116366904723 1102 76-77 0.5115193342040101 1102 78-79 0.6146908329348264 1102 80-81 0.4608914070114487 1102 82-83 0.5260073836280057 1102 84-85 0.5873707684908354 1102 86-87 0.4540929376410112 1102 88-89 0.42781405486380564 1102 90-91 0.35092879092725937 1102 92-93 0.41585170467231336 1102 94-95 0.3498212417197486 1102 96-97 0.2853788983386565 1102 98-99 0.3251700350976172 1102 100-101 0.34044590251148676 1102 102-103 0.3540748894090697 1102 104-105 0.4094389685039701 1102 106-107 0.32604957207167473 1102 108-109 0.19768543248122228 1102 110-111 0.29305941099949706 1102 112-113 0.3355820043584963 1102 114-115 0.3404180318775083 1102 116-117 0.2697878422921569 1102 118-119 0.30998781860255775 1102 120-121 0.3119733807181717 1102 122-123 0.29022058522949834 1102 124-125 0.3458857091429408 1103 1 0.0703551055052074 1103 2 0.5817162654011092 1103 3 0.3141426289871454 1103 4 0.19364931725928614 1103 5 0.35496100301635636 1103 6 0.141050087277371 1103 7 0.29874165470573644 1103 8 0.21469345134061513 1103 9 0.2343868424767308 1103 10-11 0.26202478280185915 1103 12-13 0.2832365169614093 1103 14-15 0.39995176984237446 1103 16-17 0.4858627176255368 1103 18-19 0.3414645985332285 1103 20-21 0.32316377796312423 1103 22-23 0.3403729196300347 1103 24-25 0.22121704286168864 1103 26-27 0.27970672300179444 1103 28-29 0.1606879235563028 1103 30-31 0.09102269647322458 1103 32-33 0.1526581754751959 1103 34-35 0.05183943455943307 1103 36-37 -0.007014944005305779 1103 38-39 0.035506592437883455 1103 40-41 0.018208900831453434 1103 42-43 -0.019063477661028116 1103 44-45 -0.025316618618489883 1103 46-47 0.13441177339178978 1103 48-49 0.07630911876994162 1103 50-51 0.09754990424293908 1103 52-53 0.1447772633219202 1103 54-55 0.06637717963496215 1103 56-57 0.07612477245150018 1103 58-59 0.04096454492868418 1103 60-61 -0.060222956491038815 1103 62-63 -0.17673488795359305 1103 64-65 -0.03951432498707419 1103 66-67 -0.1734470264691268 1103 68-69 -0.05753364448548126 1103 70-71 -0.07090119557155816 1103 72-73 0.019516271082842707 1103 74-75 -0.04351659614327019 1103 76-77 -0.0047696543314117434 1103 78-79 -0.11183597271362444 1103 80-81 -0.04553681451784897 1103 82-83 -0.059786235016780154 1103 84-85 -0.08868760951072474 1103 86-87 -0.14556157047463003 1103 88-89 -0.16453260514580492 1103 90-91 -0.164929215911922 1103 92-93 -0.08218650432790398 1103 94-95 -0.015106033062874502 1103 96-97 -0.10210779539585957 1103 98-99 -0.003287474346301167 1103 100-101 -0.07770996684688924 1103 102-103 0.019372855552276746 1103 104-105 -0.07535891756614177 1103 106-107 -0.21478064469092573 1103 108-109 -0.07745398891995947 1103 110-111 0.009224992754134576 1103 112-113 -0.04941182530246735 1103 114-115 -0.06108734244069325 1103 116-117 -0.03654476253295158 1103 118-119 -0.006879109322230903 1103 120-121 -0.03280491099285143 1103 122-123 0.09756587928618643 1103 124-125 0.2867317050168232 1104 1 0.20860475866714268 1104 2 0.5050842449094368 1104 3 0.31247203608847585 1104 4 0.17532648314697497 1104 5 0.4501619683927629 1104 6 0.29298058920787184 1104 7 0.4253301479608993 1104 8 0.33843987947301457 1104 9 0.35284565216361585 1104 10-11 0.3830222840625197 1104 12-13 0.30207465334341066 1104 14-15 0.42573475834466024 1104 16-17 0.5331985246104693 1104 18-19 0.47555953306675747 1104 20-21 0.4023978365831482 1104 22-23 0.4128732394987722 1104 24-25 0.3237908572776149 1104 26-27 0.40212695024658274 1104 28-29 0.4400784042625716 1104 30-31 0.15964884141515512 1104 32-33 0.2711148527038034 1104 34-35 0.2359003751993214 1104 36-37 0.3236960928460775 1104 38-39 0.34574069872461877 1104 40-41 0.36538301730381306 1104 42-43 0.4666511843694323 1104 44-45 0.3688559373961695 1104 46-47 0.3499299858398217 1104 48-49 0.417209103968176 1104 50-51 0.3238127585759685 1104 52-53 0.3416560343234991 1104 54-55 0.4348116542097884 1104 56-57 0.33384364420545865 1104 58-59 0.3234889239442893 1104 60-61 0.4450456579881106 1104 62-63 0.44141691046136344 1104 64-65 0.3158096706877913 1104 66-67 0.32728842075754727 1104 68-69 0.28943864108330075 1104 70-71 0.4782694663184017 1104 72-73 0.34919124681044167 1104 74-75 0.2471266787105364 1104 76-77 0.28310349450664773 1104 78-79 0.28212314931567306 1104 80-81 0.2708416566851817 1104 82-83 0.20538758542019764 1104 84-85 0.26930653751850997 1104 86-87 0.2367609149180261 1104 88-89 0.19540847628650582 1104 90-91 0.15968430738580963 1104 92-93 0.1494329597476991 1104 94-95 0.21494412988202427 1104 96-97 0.23472245590807717 1104 98-99 0.16700198804842614 1104 100-101 0.18209294383321506 1104 102-103 0.2381573668806496 1104 104-105 0.23186196246176038 1104 106-107 0.15060667245077752 1104 108-109 0.15254461871022684 1104 110-111 0.2501475569223146 1104 112-113 0.26435770864250685 1104 114-115 0.2925259199444987 1104 116-117 0.32916562203707755 1104 118-119 0.3226799610263136 1104 120-121 0.2645055573702493 1104 122-123 0.3647319802242208 1104 124-125 0.4663034446762815 1105 1 0.170010578531695 1105 2 0.5459374164967628 1105 3 0.20655554373787766 1105 4 0.20069942302060184 1105 5 0.36797489890518875 1105 6 0.24440720636636826 1105 7 0.32780217897830255 1105 8 0.3014561626443353 1105 9 0.24321990694735263 1105 10-11 0.16800715656449228 1105 12-13 0.32181507319782554 1105 14-15 0.3472858938720833 1105 16-17 0.3736885879915519 1105 18-19 0.3071804674008689 1105 20-21 0.18251217344623427 1105 22-23 0.3207199957723432 1105 24-25 0.2150051466109133 1105 26-27 0.14848193496226259 1105 28-29 0.18702268585117565 1105 30-31 0.16972925600993705 1105 32-33 0.12185409758349408 1105 34-35 0.0687315696926234 1105 36-37 0.055858553006721934 1105 38-39 0.03025817496575911 1105 40-41 0.11969622406799374 1105 42-43 -0.062131052039354984 1105 44-45 0.007986033879205934 1105 46-47 -0.04432874263002873 1105 48-49 -0.03721816220100038 1105 50-51 0.02420608558808368 1105 52-53 0.0015614090487545695 1105 54-55 -0.05955492101633553 1105 56-57 -0.07682389585104943 1105 58-59 -0.07680681019777325 1105 60-61 -0.1810983138125053 1105 62-63 -0.17651840319898326 1105 64-65 -0.01722539519040822 1105 66-67 0.03435377058395517 1105 68-69 -0.01342134873519285 1105 70-71 -0.06164245883064012 1105 72-73 -0.009131229466888158 1105 74-75 3.4905293153997263E-4 1105 76-77 -0.12064007871936866 1105 78-79 -0.15092922753515126 1105 80-81 -0.0662872431090129 1105 82-83 -0.08075971587758346 1105 84-85 -0.10406556756814211 1105 86-87 -0.07832140569324864 1105 88-89 -0.02864828681494558 1105 90-91 -0.0036137767057091708 1105 92-93 0.07028200565374476 1105 94-95 0.0883262475869877 1105 96-97 0.10584648691582643 1105 98-99 0.04300572287569082 1105 100-101 0.05437711159161651 1105 102-103 0.050843414598613634 1105 104-105 0.024025098172547388 1105 106-107 0.16607465981799407 1105 108-109 0.10903351917997384 1105 110-111 0.19416718889224427 1105 112-113 0.15524532122414314 1105 114-115 0.040341359643203845 1105 116-117 0.08599971657067229 1105 118-119 0.11882118237273076 1105 120-121 0.09792755666092035 1105 122-123 0.09865997864149989 1105 124-125 0.45667637248650905 1106 1 0.11223039032149629 1106 2 0.45693732238216 1106 3 0.24391595229032959 1106 4 0.049627419643883286 1106 5 0.35574736781646976 1106 6 0.25354844386885134 1106 7 0.2939522927238798 1106 8 0.18170245771358395 1106 9 0.10345092502971198 1106 10-11 0.16109935845648238 1106 12-13 0.17360881200983158 1106 14-15 0.1021114063451165 1106 16-17 0.10125841579984751 1106 18-19 0.12896804598418754 1106 20-21 0.24028594475154819 1106 22-23 0.10078224450298023 1106 24-25 0.1566732234122341 1106 26-27 0.09856566368785025 1106 28-29 0.09045123813088907 1106 30-31 0.0357856354115782 1106 32-33 0.021587819797105112 1106 34-35 0.09275919470581329 1106 36-37 0.07888169474236406 1106 38-39 -0.04923064013136269 1106 40-41 -0.07638050405202534 1106 42-43 -0.07967213686702479 1106 44-45 -0.172148250550336 1106 46-47 -0.21778611869582676 1106 48-49 -0.20230682481344786 1106 50-51 -0.30181344176646974 1106 52-53 -0.26108298319429935 1106 54-55 -0.24017213695421447 1106 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2216 64-65 -1.1270872969403527 2216 66-67 -1.1170479278798737 2216 68-69 -1.0312076580576246 2216 70-71 -1.0052829032554698 2216 72-73 -1.0019864285607767 2216 74-75 -0.7682855076693933 2216 76-77 -0.7502838867301946 2216 78-79 -0.7253895092722544 2216 80-81 -0.7359143312535998 2216 82-83 -0.7408660414785899 2216 84-85 -0.6922966939771982 2216 86-87 -0.5255904533473164 2216 88-89 -0.7230104803044952 2216 90-91 -0.6344187818785691 2216 92-93 -0.6290246837637063 2216 94-95 -0.8314801746067957 2216 96-97 -0.7496861462424604 2216 98-99 -0.9106733831753324 2216 100-101 -0.9863885945407986 2216 102-103 -0.9007002311167334 2216 104-105 -0.817253358696572 2216 106-107 -0.6548493211772595 2216 108-109 -0.7192338417779105 2216 110-111 -0.7737785965737345 2216 112-113 -0.6357731441765182 2216 114-115 -0.80046492035817 2216 116-117 -0.8691901838456069 2216 118-119 -0.8040054801752596 2216 120-121 -0.8065816588782582 2216 122-123 -0.8932187508447917 2216 124-125 -1.0378497100145054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64.0 3 79.0 4 189.0 5 435.0 6 1042.0 7 2124.0 8 3292.0 9 5517.0 10 8936.0 11 11322.0 12 12310.0 13 15631.0 14 21259.0 15 16076.0 16 8088.0 17 7206.0 18 7099.0 19 6867.0 20 6687.0 21 6730.0 22 7401.0 23 8927.0 24 11093.0 25 14490.0 26 18219.0 27 23026.0 28 29412.0 29 36877.0 30 46432.0 31 58025.0 32 72919.0 33 93149.0 34 125855.0 35 178617.0 36 293917.0 37 461346.0 38 184131.0 39 859.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.70403981345754 18.18040750049185 11.005561359221687 22.10999132682892 2 19.324074956082153 21.535529705744874 36.2061956841991 22.93419965397387 3 17.76616235962088 27.66001752281455 32.15309091593812 22.420729201626454 4 11.671286437810311 16.32203450844435 36.66241149284605 35.34426756089929 5 10.367039438354123 41.730570252564405 35.4139383987351 12.488451910346368 6 30.29484722096844 37.179027794469235 18.10852287296465 14.417602111597672 7 26.36028727636837 34.64102637944937 22.45581641604565 16.542869928136604 8 27.701412456912976 32.68976013043517 20.397331041266074 19.211496371385785 9 27.26123807076462 11.38344793669641 19.69198869325583 41.66332529928314 10-11 24.39423409213756 25.622075842024582 29.751756710056444 20.231933355781415 12-13 25.609974923130093 25.137872940905424 29.680729577414866 19.571422558549617 14-15 22.85503597600418 26.14587671572754 27.73159552692441 23.26749178134387 16-17 21.27364283449566 28.377548774054734 28.24349713883801 22.1053112526116 18-19 20.555501404481937 26.095783895864532 31.881490744596952 21.46722395505658 20-21 21.96804139012698 25.362141458357335 32.05353424366211 20.616282907853577 22-23 21.547665990270527 25.65336654763276 32.08072669756232 20.718240764534393 24-25 21.19652368471698 25.73913236110592 32.1952097516265 20.86913420255061 26-27 20.954499437321118 25.74142357757437 32.190299548970785 21.11377743613373 28-29 21.008745890671936 25.651982003136826 32.30928730295163 21.029984803239614 30-31 21.14949868412891 25.434220844815826 32.40313172888625 21.013148742169015 32-33 20.838751517460768 25.78822118153704 32.03232302198435 21.34070427901784 34-35 21.385644508453634 25.790556109220898 31.785177971177585 21.038621411147883 36-37 21.55242882333655 25.59701558664812 31.42921543955411 21.42134015046122 38-39 21.24467227277963 25.855510044039594 31.53397007611672 21.365847607064055 40-41 21.263070799552736 25.78405413682741 31.558161700487414 21.39471336313244 42-43 21.236053470270157 25.91953429369659 31.163437779598656 21.6809744564346 44-45 21.419857826454557 26.091938966567973 30.806308276046757 21.68189493093071 46-47 21.749050924925864 26.316353557743753 30.112321525698416 21.822273991631967 48-49 21.763247745136837 26.39925582384582 29.701800595019677 22.135695835997666 50-51 21.87559453505455 26.70756006275244 29.497218925980643 21.91962647621237 52-53 21.984889763731555 26.51491444899035 29.443692741913598 22.056503045364494 54-55 21.694423259376677 26.882078651156426 29.370341015897385 22.05315707356951 56-57 21.933353881071792 26.960239018383025 29.194320959551696 21.912086140993488 58-59 22.01445016835269 26.382260148998842 29.251137235615392 22.35215244703308 60-61 22.069007913970083 26.556341657323074 29.62927374184103 21.74537668686581 62-63 21.60866207341141 26.532376578994953 29.83753212541852 22.021429222175115 64-65 21.788008224356002 26.602413308503348 29.598571121987693 22.01100734515296 66-67 21.854605943243687 26.834151484010967 29.38322598653686 21.92801658620849 68-69 21.83135331279377 26.581602672986193 29.509791922003565 22.077252092216472 70-71 21.76491617599372 26.67345481290553 29.46427458817672 22.097354422924035 72-73 21.777476123581117 26.84202670636539 28.8996258583368 22.48087131171669 74-75 21.679678738346873 27.053451120411648 28.94470132601844 22.322168815223044 76-77 21.37890739117733 27.002615427004113 29.14873320013901 22.46974398167955 78-79 21.718629544628854 26.896355214305334 28.912722745518504 22.472292495547304 80-81 21.60793778611707 27.021026316401592 28.813463576943825 22.557572320537513 82-83 21.68627690968304 26.803169944947697 28.905879417704405 22.604673727664856 84-85 21.67485720441029 26.58178635226669 28.917965674784103 22.82539076853892 86-87 21.44478845883859 26.784497365617703 29.00955145304314 22.761162722500565 88-89 21.650293291877688 26.740471233083067 28.844867800586442 22.7643676744528 90-91 21.595159095553697 26.807571722179667 28.765023291258977 22.832245891007652 92-93 21.53027478121981 26.67726865290993 28.77569765564664 23.016758910223626 94-95 21.452981252203184 26.65945956124346 28.801875028902728 23.085684157650633 96-97 21.794068747500862 26.70182352674037 28.64569660759561 22.85841111816316 98-99 21.656020265637583 26.900512733036557 28.347413461762123 23.09605353956374 100-101 21.437614572199177 26.682432170488973 28.65886145002021 23.22109180729164 102-103 21.550446285047006 26.241582916973233 28.56337834720708 23.64459245077268 104-105 21.367785498057746 26.33811724856754 28.832033512882454 23.46206374049226 106-107 21.35582510009617 26.381194242369112 28.721889791339738 23.541090866194978 108-109 21.424684770253 26.612521131850286 28.268510531657277 23.694283566239438 110-111 21.300982262127082 26.58116732793244 28.375363031587902 23.74248737835257 112-113 21.265904044284014 26.354533556141806 28.54339420317629 23.83616819639788 114-115 21.015763179512575 26.639479793472827 28.21491145709565 24.129845569918952 116-117 20.97311007644417 26.707516654392656 28.406925258315148 23.91244801084803 118-119 21.014616373506527 26.505103185315942 28.23548374053763 24.2447967006399 120-121 20.878547247010932 26.68825765180729 28.22381146888392 24.20938363229786 122-123 20.96513748968104 26.452844599289243 28.118112327340008 24.463905583689712 124-125 21.00910218489545 26.539339278133518 27.800031624616356 24.651526912354672 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 138.0 1 164.5 2 701.5 3 1371.0 4 1088.5 5 937.0 6 1530.5 7 3447.0 8 5068.5 9 5612.5 10 4826.5 11 3192.5 12 2818.5 13 2812.0 14 3041.5 15 3227.5 16 3405.0 17 3714.0 18 3949.5 19 4157.5 20 4572.0 21 5047.5 22 5913.5 23 7292.0 24 8706.0 25 10500.5 26 12823.5 27 15357.0 28 17405.0 29 20023.0 30 24700.5 31 28707.0 32 31715.5 33 35365.5 34 39163.0 35 41964.5 36 44966.0 37 47814.5 38 49859.5 39 51764.0 40 51773.5 41 53075.0 42 54586.0 43 56586.0 44 61015.5 45 64646.5 46 66976.0 47 67332.0 48 64732.0 49 61428.0 50 61999.5 51 60793.5 52 57543.0 53 56478.0 54 55596.5 55 52132.5 56 46388.5 57 42111.5 58 38849.0 59 35419.5 60 29960.5 61 24589.5 62 20633.0 63 15793.5 64 11727.5 65 9221.5 66 6689.5 67 5111.5 68 3866.0 69 2436.0 70 1726.5 71 1322.5 72 1139.5 73 984.5 74 759.5 75 607.0 76 378.0 77 210.5 78 115.0 79 42.0 80 23.5 81 25.5 82 20.5 83 6.0 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06828573453962233 2 2.2152711934995083E-4 3 1.1076355967497541E-4 4 1.661453395124631E-4 5 0.0685072616589723 6 5.538177983748771E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.7690889918743854E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.7690889918743854E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.492180092686947E-4 36-37 0.0 38-39 0.0 40-41 5.538177983748771E-5 42-43 0.002575252762443178 44-45 0.009885647700991556 46-47 0.011408646646522467 48-49 0.008944157443754264 50-51 0.0077811400671670234 52-53 0.006479668240986062 54-55 0.00725501315871089 56-57 0.005344341754317564 58-59 0.003516743019680469 60-61 0.0024091074229307154 62-63 0.0016337625052058875 64-65 0.0019660531842308135 66-67 0.002852161661630617 68-69 0.001495308055612168 70-71 0.0027413981019556413 72-73 0.0026860163221181536 74-75 9.414902572372909E-4 76-77 8.584175874810595E-4 78-79 0.0 80-81 3.322906790249262E-4 82-83 8.307266975623155E-5 84-85 4.1536334878115785E-4 86-87 3.5998156894367006E-4 88-89 0.0013014718261809611 90-91 0.0019383622943120696 92-93 6.368904681311086E-4 94-95 0.0012460900463434734 96-97 0.0014399262757746802 98-99 0.0016614533951246314 100-101 9.968720370747788E-4 102-103 0.0010799447068310102 104-105 7.753449177248278E-4 106-107 5.261269084561333E-4 108-109 0.001135326486668498 110-111 0.001107635596749754 112-113 0.011685555545709906 114-115 0.020214349640683013 116-117 0.019023641374177027 118-119 0.011464028426359954 120-121 0.0061196866720423905 122-123 0.006230450231717368 124-125 0.005122814634967613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1805648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.804395504559544 #Duplication Level Percentage of deduplicated Percentage of total 1 39.973743407785456 9.515507978767047 2 15.488927348136391 7.374091050728546 3 11.848457084924528 8.461360757050322 4 8.816564454292104 8.394919490456418 5 6.383409947681497 7.597660753117504 6 4.4011596050740085 6.286016635072368 7 2.976629454991029 4.959980537198958 8 2.097230432972711 3.9938642152544857 9 1.4489828786838932 3.1042945371173953 >10 6.041847181563488 24.025735924411773 >50 0.3333024284800823 5.495954065885492 >100 0.18077787002104515 7.654826511387802 >500 0.0070799253108782805 1.067995789793183 >1k 0.0011799875518130468 0.5164423384129573 >5k 4.719950207252187E-4 0.9384498273891494 >10k+ 2.3599751036260935E-4 0.612899587956637 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10910 0.6042152180269909 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8814 0.4881350074876166 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7891 0.43701762469761546 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 0.12549511311174716 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2179 0.12067689826588571 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1939 0.10738527110488866 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.7690889918743854E-5 0.0 16-17 0.0 0.0 0.0 5.538177983748771E-5 0.0 18-19 1.6614533951246312E-4 0.0 0.0 8.307266975623156E-5 0.0 20-21 3.3229067902492624E-4 0.0 0.0 1.3845444959371928E-4 0.0 22-23 3.3229067902492624E-4 0.0 0.0 1.9383622943120696E-4 0.0 24-25 3.3229067902492624E-4 0.0 0.0 4.4305423869990166E-4 0.0 26-27 3.3229067902492624E-4 0.0 0.0 6.922722479685962E-4 0.0 28-29 3.3229067902492624E-4 0.0 0.0 0.0010799447068310102 0.0 30-31 3.3229067902492624E-4 0.0 0.0 0.0028521616616306172 0.0 32-33 3.3229067902492624E-4 0.0 0.0 0.008584175874810595 0.0 34-35 3.3229067902492624E-4 0.0 0.0 0.015506898354496559 0.0 36-37 3.3229067902492624E-4 0.0 0.0 0.02843854394654994 0.0 38-39 3.3229067902492624E-4 0.0 0.0 0.05128352812951362 0.0 40-41 3.3229067902492624E-4 0.0 0.0 0.07850367291963882 0.0 42-43 3.3229067902492624E-4 0.0 0.0 0.11098508679432537 0.0 44-45 3.3229067902492624E-4 0.0 0.0 0.14759244326690474 0.0 46-47 3.3229067902492624E-4 0.0 0.0 0.19463926523885056 0.0 48-49 3.3229067902492624E-4 0.0 0.0 0.24320908615632725 0.0 50-51 3.3229067902492624E-4 0.0 0.0 0.29374496025803476 0.0 52-53 3.3229067902492624E-4 0.0 0.0 0.35258810133536544 0.0 54-55 3.3229067902492624E-4 0.0 0.0 0.42259067104994996 0.0 56-57 3.3229067902492624E-4 0.0 0.0 0.5098723560738305 0.0 58-59 3.3229067902492624E-4 0.0 0.0 0.6135747388195263 0.0 60-61 3.3229067902492624E-4 0.0 0.0 0.7211815370437649 0.0 62-63 3.3229067902492624E-4 0.0 0.0 0.828843717047841 0.0 64-65 3.3229067902492624E-4 0.0 0.0 0.9485791250564894 0.0 66-67 3.3229067902492624E-4 0.0 0.0 1.0954516051855068 0.0 68-69 3.3229067902492624E-4 0.0 0.0 1.273919390711811 0.0 70-71 3.3229067902492624E-4 0.0 0.0 1.4616636243608943 0.0 72-73 3.3229067902492624E-4 0.0 0.0 1.6461403329995656 0.0 74-75 3.3229067902492624E-4 0.0 0.0 1.8458193402036278 0.0 76-77 3.3229067902492624E-4 0.0 0.0 2.084376356853606 0.0 78-79 3.3229067902492624E-4 0.0 0.0 2.377013681514891 0.0 80-81 3.3229067902492624E-4 0.0 0.0 2.677238310013912 0.0 82-83 3.3229067902492624E-4 0.0 0.0 2.9824196078083878 0.0 84-85 3.3229067902492624E-4 0.0 0.0 3.2957420272389744 0.0 86-87 3.3229067902492624E-4 0.0 0.0 3.631327922164231 0.0 88-89 3.3229067902492624E-4 0.0 0.0 4.031876644838861 0.0 90-91 3.3229067902492624E-4 0.0 0.0 4.459783966753211 0.0 92-93 3.3229067902492624E-4 0.0 0.0 4.900124498241075 0.0 94-95 3.3229067902492624E-4 0.0 0.0 5.358768707965229 0.0 96-97 3.3229067902492624E-4 0.0 0.0 5.854380255730907 0.0 98-99 3.3229067902492624E-4 0.0 0.0 6.39858931530398 0.0 100-101 3.3229067902492624E-4 0.0 0.0 6.994220357456159 0.0 102-103 3.3229067902492624E-4 0.0 0.0 7.576089027318725 0.0 104-105 3.3229067902492624E-4 0.0 0.0 8.186700840916945 0.0 106-107 3.3229067902492624E-4 0.0 0.0 8.826000416470984 0.0 108-109 3.3229067902492624E-4 0.0 0.0 9.505424091517284 0.0 110-111 3.3229067902492624E-4 0.0 0.0 10.212067911353708 0.0 112-113 3.3229067902492624E-4 0.0 0.0 10.930424977625762 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6195 0.0 77.04162 1 GTATCAA 9010 0.0 52.86428 2 GTACATG 14540 0.0 46.184235 1 TACATGG 14740 0.0 45.27988 2 ACATGGG 14820 0.0 43.86918 3 CATGGGA 9880 0.0 41.519047 4 ATGGGAT 3885 0.0 39.84441 5 TCAACGC 13210 0.0 37.272438 5 ATCAACG 13310 0.0 37.03713 4 CAACGCA 13340 0.0 36.97286 6 ATGGGAG 3530 0.0 36.261757 5 AACGCAG 13655 0.0 35.945667 7 TATCAAC 13765 0.0 35.89938 3 CATGGGG 5210 0.0 34.853565 4 CGTCTAG 75 0.0038839534 31.77847 1 ACGCAGA 15725 0.0 31.213871 6 CGCAGAG 15905 0.0 30.860617 7 AGTACAT 9400 0.0 30.08589 2 GAGTACA 10135 0.0 28.983938 1 CAGAGTA 17320 0.0 28.20198 9 >>END_MODULE