FastQCFastQC Report
Thu 26 May 2016
SRR936248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022113
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.056.8644071
GTACATG297100.054.446851
TACATGG306250.051.9265022
ACATGGG301550.051.0594983
CATGGGA196350.046.692094
CATGGGG102350.041.7938774
GTATCAA75800.041.048632
ATGGGAG76900.038.6825645
ATCAACG87600.035.9271854
TCAACGC87800.035.9131055
ATGGGAT92400.035.8636785
CAACGCA89050.035.6094176
ATGGGGG66300.035.086085
AACGCAG92550.034.3274737
AGTACAT167900.032.244592
GAGTACA174550.032.0727351
TATCAAC104800.029.9739673
ACGCAGA112450.027.7768028
CGCAGAG114100.026.7494039
TGGGGGG61650.026.5381856
Produced by FastQC (version 0.11.5)