##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936248_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4022113 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.684901692219984 31.0 30.0 33.0 26.0 34.0 2 29.78873368301686 31.0 30.0 34.0 25.0 34.0 3 29.85617062474376 31.0 30.0 34.0 26.0 34.0 4 33.33975897743301 35.0 35.0 37.0 27.0 37.0 5 33.57584433853549 35.0 35.0 37.0 28.0 37.0 6 33.70713602526831 37.0 35.0 37.0 29.0 37.0 7 33.69376593845076 37.0 35.0 37.0 29.0 37.0 8 33.63654725762304 37.0 35.0 37.0 28.0 37.0 9 35.03985193852087 39.0 35.0 39.0 28.0 39.0 10-11 34.88074738327839 39.0 35.0 39.0 27.0 39.0 12-13 34.87119954113671 39.0 35.0 39.0 27.0 39.0 14-15 36.00720268177448 40.0 36.0 41.0 27.0 41.0 16-17 36.02386643537862 40.0 36.0 41.0 27.0 41.0 18-19 36.056193970681576 40.0 36.0 41.0 27.0 41.0 20-21 36.147984156586354 40.0 36.0 41.0 27.0 41.0 22-23 36.03832139972199 40.0 36.0 41.0 27.0 41.0 24-25 36.01041194019163 39.0 36.0 41.0 27.0 41.0 26-27 36.09457615934709 39.0 36.0 41.0 28.0 41.0 28-29 35.99520127853195 39.0 35.5 41.0 27.5 41.0 30-31 35.92626015231298 39.0 35.0 41.0 27.5 41.0 32-33 35.75848043055976 39.0 35.0 41.0 27.0 41.0 34-35 35.728253656722224 39.0 35.0 41.0 27.0 41.0 36-37 35.63735255573376 39.0 35.0 41.0 27.0 41.0 38-39 35.41832104170122 39.0 35.0 41.0 26.5 41.0 40-41 35.533145140377705 39.0 35.0 41.0 27.0 41.0 42-43 35.474276953432195 39.0 35.0 41.0 26.5 41.0 44-45 35.39034519915278 39.0 35.0 41.0 26.5 41.0 46-47 35.38378111206721 39.0 35.0 41.0 27.0 41.0 48-49 35.30930247360032 39.0 34.5 41.0 27.0 41.0 50-51 35.2004177903505 39.0 34.0 41.0 26.0 41.0 52-53 35.069264588041165 39.0 34.0 40.5 26.0 41.0 54-55 34.926233798006166 39.0 34.0 40.0 26.0 41.0 56-57 34.75480027537765 38.0 34.0 40.0 26.0 41.0 58-59 34.544481594624514 38.0 33.5 40.0 25.5 41.0 60-61 34.3240187433819 38.0 33.0 40.0 25.0 41.0 62-63 34.09520654939332 38.0 33.0 40.0 24.0 41.0 64-65 33.83691930087494 37.0 33.0 40.0 23.5 41.0 66-67 33.56073561334552 37.0 32.0 40.0 23.0 41.0 68-69 33.28006808858926 36.0 32.0 39.0 22.5 41.0 70-71 32.94553957086735 36.0 32.0 39.0 22.0 41.0 72-73 32.61652357852701 35.5 32.0 39.0 22.0 40.0 74-75 32.270228608693984 35.0 31.0 38.0 21.0 40.0 76-77 31.93139949076518 35.0 31.0 37.0 20.0 39.0 78-79 31.582287593610623 35.0 31.0 37.0 20.0 39.0 80-81 31.24586977044156 35.0 31.0 36.0 18.5 39.0 82-83 30.91795929154651 35.0 31.0 36.0 18.0 37.5 84-85 30.910945192240995 35.0 31.0 36.0 18.0 37.0 86-87 30.76116546203451 35.0 31.0 35.0 18.0 37.0 88-89 30.5696196253064 35.0 31.0 35.0 17.5 36.0 90-91 30.383888021047643 35.0 31.0 35.0 15.5 36.0 92-93 30.23993035501489 34.0 31.0 35.0 12.0 36.0 94-95 30.101837143809732 34.0 31.0 35.0 8.5 36.0 96-97 29.987096707626065 34.0 31.0 35.0 7.0 35.0 98-99 29.882948838085852 34.0 31.0 35.0 7.0 35.0 100-101 29.774621573287476 34.0 31.0 35.0 2.0 35.0 102-103 28.809710343791934 33.5 29.0 34.5 2.0 35.0 104-105 29.465080543485477 34.0 30.0 35.0 2.0 35.0 106-107 29.569987466786735 34.0 30.0 35.0 2.0 35.0 108-109 29.51863808898457 34.0 30.0 35.0 2.0 35.0 110-111 29.405026785672106 34.0 30.0 35.0 2.0 35.0 112-113 29.358340379795393 34.0 30.0 35.0 2.0 35.0 114-115 29.24149905783353 34.0 30.0 35.0 2.0 35.0 116-117 29.112275189682638 34.0 30.0 35.0 2.0 35.0 118-119 28.91236223348275 34.0 30.0 35.0 2.0 35.0 120-121 28.714476196964135 34.0 30.0 35.0 2.0 35.0 122-123 28.427990958981013 34.0 29.5 35.0 2.0 35.0 124-125 26.46012394480215 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39235423219474796 1101 2 0.4893194222227244 1101 3 0.4349461333953535 1101 4 0.347137005794238 1101 5 0.3259855914983447 1101 6 0.3489889077287742 1101 7 0.23641373769071805 1101 8 0.31357123295977374 1101 9 0.3720646546695008 1101 10-11 0.45388107463872274 1101 12-13 0.39495273376964946 1101 14-15 0.5135143632274222 1101 16-17 0.48500824313861557 1101 18-19 0.43462875412873814 1101 20-21 0.4318253181174043 1101 22-23 0.5544571033129984 1101 24-25 0.5827221131119416 1101 26-27 0.5178569887037838 1101 28-29 0.5674742723936177 1101 30-31 0.5115672163199889 1101 32-33 0.6390083227742736 1101 34-35 0.5990760780900075 1101 36-37 0.5795739769465555 1101 38-39 0.7312078674634606 1101 40-41 0.646450710946084 1101 42-43 0.7580802193686296 1101 44-45 0.6964179780138409 1101 46-47 0.5182476845541473 1101 48-49 0.600105459508697 1101 50-51 0.5329518896470233 1101 52-53 0.5590136491579756 1101 54-55 0.6085812933838 1101 56-57 0.679075311914815 1101 58-59 0.6530626033927334 1101 60-61 0.6769406902993609 1101 62-63 0.723153718440777 1101 64-65 0.7413538505501762 1101 66-67 0.692155126739948 1101 68-69 0.6214464649224993 1101 70-71 0.6224011075565414 1101 72-73 0.6107372644561053 1101 74-75 0.5353727341791057 1101 76-77 0.4946183615450366 1101 78-79 0.5062055136691157 1101 80-81 0.4179969884955739 1101 82-83 0.38547870755259694 1101 84-85 0.28062531504450305 1101 86-87 0.415409614590029 1101 88-89 0.4150061446424509 1101 90-91 0.33918283571374985 1101 92-93 0.3309703582070753 1101 94-95 0.47550388655715636 1101 96-97 0.4170982303019599 1101 98-99 0.42257792692003093 1101 100-101 0.46883577137803556 1101 102-103 0.5479456105825165 1101 104-105 0.4801120615493879 1101 106-107 0.4668396418473044 1101 108-109 0.5076122853112892 1101 110-111 0.5257712267761647 1101 112-113 0.42724473487521664 1101 114-115 0.49349736342738026 1101 116-117 0.48172355595633576 1101 118-119 0.5570855745276688 1101 120-121 0.5678339918281132 1101 122-123 0.5563926045718652 1101 124-125 0.38804254440429276 1102 1 0.2122900341038232 1102 2 0.14279613587476092 1102 3 0.24069229080228283 1102 4 0.18685403613288543 1102 5 0.24923355384871826 1102 6 0.136314913427924 1102 7 0.29039541252490153 1102 8 0.22875388054677614 1102 9 0.3116973001019403 1102 10-11 0.351825937350668 1102 12-13 0.27052477064204083 1102 14-15 0.39425103658513194 1102 16-17 0.3272545735716079 1102 18-19 0.34916344618595474 1102 20-21 0.27970241118428163 1102 22-23 0.39553314833625564 1102 24-25 0.51745641565563 1102 26-27 0.2929772543787692 1102 28-29 0.3373031259511734 1102 30-31 0.3572014077515959 1102 32-33 0.4230576985189529 1102 34-35 0.2222178323073365 1102 36-37 0.27033311593499576 1102 38-39 0.27874715654177606 1102 40-41 0.2584250533875405 1102 42-43 0.3639667935767221 1102 44-45 0.40404514953065984 1102 46-47 0.3587439005837396 1102 48-49 0.30212712834712363 1102 50-51 0.23980744949408006 1102 52-53 0.28739893921918735 1102 54-55 0.3392628204650876 1102 56-57 0.3628579314263902 1102 58-59 0.44053073103918194 1102 60-61 0.4813110987968514 1102 62-63 0.3828113356722227 1102 64-65 0.35642871703987566 1102 66-67 0.31817128147945084 1102 68-69 0.33737546422378983 1102 70-71 0.27794966628507467 1102 72-73 0.2242670702782874 1102 74-75 0.24829084195860673 1102 76-77 0.16914867309506576 1102 78-79 0.20862038391807403 1102 80-81 0.21355912758949813 1102 82-83 0.16367468673870889 1102 84-85 0.20071328472303662 1102 86-87 0.20640282123497045 1102 88-89 0.190345528204368 1102 90-91 0.20239436080966655 1102 92-93 0.2281675077314702 1102 94-95 0.210808289508833 1102 96-97 0.24216702435652593 1102 98-99 0.19971244603048888 1102 100-101 0.271298861573392 1102 102-103 0.27093514415763664 1102 104-105 0.23517145482931312 1102 106-107 0.1962895511308851 1102 108-109 0.17674903459426616 1102 110-111 0.18433221033617642 1102 112-113 0.1464695040658448 1102 114-115 0.13192357170846591 1102 116-117 0.1668894983985325 1102 118-119 0.23958695516730444 1102 120-121 0.17489432782913994 1102 122-123 0.20395047842118785 1102 124-125 0.15374191735993392 1103 1 0.12458482505422808 1103 2 0.22760962857713096 1103 3 0.24546038167573414 1103 4 0.2415032029773343 1103 5 0.24721061606434347 1103 6 0.3083731199790165 1103 7 0.31895549989182825 1103 8 0.22988305739475123 1103 9 0.3011507994451321 1103 10-11 0.4357841208228095 1103 12-13 0.2744155572219853 1103 14-15 0.32869615163226484 1103 16-17 0.30125455823851865 1103 18-19 0.32326288449230844 1103 20-21 0.2427489997420338 1103 22-23 0.37717246525207315 1103 24-25 0.43106800240117593 1103 26-27 0.3000384495619528 1103 28-29 0.3085486254889389 1103 30-31 0.28343095690303244 1103 32-33 0.39670566964620235 1103 34-35 0.3539073915521769 1103 36-37 0.3210577633861149 1103 38-39 0.3764748180039206 1103 40-41 0.3765522504154575 1103 42-43 0.4880179855003064 1103 44-45 0.45444287156117014 1103 46-47 0.40164106811130296 1103 48-49 0.3490409328136508 1103 50-51 0.3010488434629437 1103 52-53 0.2899340553158467 1103 54-55 0.3292658659353833 1103 56-57 0.3967300777189422 1103 58-59 0.42181465055945466 1103 60-61 0.39516866278543716 1103 62-63 0.40469646328192255 1103 64-65 0.3381438931312388 1103 66-67 0.4735996075784712 1103 68-69 0.3892158720630192 1103 70-71 0.38189996114317637 1103 72-73 0.40446474562216395 1103 74-75 0.3150157083029086 1103 76-77 0.36269893802718656 1103 78-79 0.3578268697123548 1103 80-81 0.35879292691679865 1103 82-83 0.34674631318639726 1103 84-85 0.38085459771728125 1103 86-87 0.3343418124282209 1103 88-89 0.37881223701061373 1103 90-91 0.33255001553687435 1103 92-93 0.2903545704573176 1103 94-95 0.3820711973989539 1103 96-97 0.41914539702322173 1103 98-99 0.3272618443782669 1103 100-101 0.3125141205427937 1103 102-103 0.25061511598703845 1103 104-105 0.27482218274165504 1103 106-107 0.2816938835767502 1103 108-109 0.2721881123666421 1103 110-111 0.14794613670083123 1103 112-113 0.2654366772270045 1103 114-115 0.27076562415453154 1103 116-117 0.24613560967399195 1103 118-119 0.20505478514018094 1103 120-121 0.16814516116974687 1103 122-123 0.18217052792594046 1103 124-125 0.10102977008395442 1104 1 0.30771404627160237 1104 2 0.45636889438292627 1104 3 0.43994661218263786 1104 4 0.4616734435203895 1104 5 0.40822762808934954 1104 6 0.36783852734591704 1104 7 0.3001124513453419 1104 8 0.394949173758981 1104 9 0.5130054825491541 1104 10-11 0.5748582479580833 1104 12-13 0.5689230880664056 1104 14-15 0.698572736856697 1104 16-17 0.6798236365923316 1104 18-19 0.5586008783751737 1104 20-21 0.5772026785426974 1104 22-23 0.6004370044215648 1104 24-25 0.6256825542597184 1104 26-27 0.5666932255936388 1104 28-29 0.6354912128257482 1104 30-31 0.5864383531199877 1104 32-33 0.8079220129799722 1104 34-35 0.7584903715359061 1104 36-37 0.7050672245620362 1104 38-39 0.8713863451615467 1104 40-41 0.7271794008182013 1104 42-43 0.8403758181343903 1104 44-45 0.7688886603077094 1104 46-47 0.7546091226923082 1104 48-49 0.7562541825509541 1104 50-51 0.7771830592769362 1104 52-53 0.7775176463561522 1104 54-55 0.7453849168841273 1104 56-57 0.729020867414846 1104 58-59 0.7803380684509804 1104 60-61 0.7489538750466522 1104 62-63 0.7002771983441463 1104 64-65 0.8150568073147042 1104 66-67 0.7771860131567152 1104 68-69 0.7079547956357999 1104 70-71 0.7631582104202366 1104 72-73 0.6787624849671943 1104 74-75 0.7094810942078524 1104 76-77 0.6772706104701243 1104 78-79 0.6888556958864953 1104 80-81 0.6960429009902143 1104 82-83 0.6990071297913651 1104 84-85 0.6687281690459628 1104 86-87 0.6657740308010105 1104 88-89 0.6676107395271984 1104 90-91 0.642849299719412 1104 92-93 0.6745026865234109 1104 94-95 0.701372411197589 1104 96-97 0.702309140208186 1104 98-99 0.7357042817727901 1104 100-101 0.6666864033110116 1104 102-103 0.6748868386615712 1104 104-105 0.7105808788250272 1104 106-107 0.7915019616800727 1104 108-109 0.7631473922366503 1104 110-111 0.8524353914900118 1104 112-113 0.7571117401145351 1104 114-115 0.7467185923521917 1104 116-117 0.8166466821135252 1104 118-119 0.7808106980571736 1104 120-121 0.7464375206096427 1104 122-123 0.7389974649490654 1104 124-125 0.7944003228800405 1105 1 0.30148267124615913 1105 2 0.4031882314773547 1105 3 0.4275448693556214 1105 4 0.39349454154604047 1105 5 0.2638217295726193 1105 6 0.26958486362817524 1105 7 0.3718466887613445 1105 8 0.32588215777347074 1105 9 0.457573491116797 1105 10-11 0.5262357604794019 1105 12-13 0.42652349938826717 1105 14-15 0.43507623829342634 1105 16-17 0.43124223450956123 1105 18-19 0.36740126939729123 1105 20-21 0.3133995241992764 1105 22-23 0.2477379566561453 1105 24-25 0.4073149348065783 1105 26-27 0.3028502995792053 1105 28-29 0.4124335955519598 1105 30-31 0.4389756147666475 1105 32-33 0.5769708887644143 1105 34-35 0.6728925574301314 1105 36-37 0.562301862484162 1105 38-39 0.6343454762603074 1105 40-41 0.5179841904791544 1105 42-43 0.7154224958591442 1105 44-45 0.7327144359137776 1105 46-47 0.6043500399494164 1105 48-49 0.6108486833705911 1105 50-51 0.5530713127588882 1105 52-53 0.5409400004467599 1105 54-55 0.550579403054833 1105 56-57 0.5193378692283233 1105 58-59 0.5715582111287105 1105 60-61 0.5555046471839518 1105 62-63 0.5569261489209154 1105 64-65 0.5437063935183559 1105 66-67 0.5905866008056506 1105 68-69 0.5479497638895481 1105 70-71 0.45441270790337995 1105 72-73 0.47738114824672095 1105 74-75 0.5342723064321859 1105 76-77 0.4979406857429609 1105 78-79 0.48005829223876617 1105 80-81 0.5308759202593194 1105 82-83 0.5559790227375423 1105 84-85 0.4818828381951512 1105 86-87 0.5657522373649897 1105 88-89 0.6165597506252958 1105 90-91 0.5429848833254916 1105 92-93 0.5542307238778292 1105 94-95 0.554247372123271 1105 96-97 0.5406782389515286 1105 98-99 0.534964510695648 1105 100-101 0.5408471265723485 1105 102-103 0.6564781338125414 1105 104-105 0.6033520446874725 1105 106-107 0.5392809118123338 1105 108-109 0.5688199482899634 1105 110-111 0.6083238933199624 1105 112-113 0.6157754587585735 1105 114-115 0.5849926709946445 1105 116-117 0.6011745662840262 1105 118-119 0.635562215994284 1105 120-121 0.6192039858564904 1105 122-123 0.6112477982842606 1105 124-125 0.6139568813455689 1106 1 -0.19693318163962203 1106 2 -0.3970680125500827 1106 3 -0.18574545504167617 1106 4 -0.10758016040797713 1106 5 -0.09411200264064234 1106 6 -0.14482749159400043 1106 7 -0.2884310260923755 1106 8 -0.26396569093279965 1106 9 -0.289904627922148 1106 10-11 -0.12574017572416096 1106 12-13 -0.1971089452768453 1106 14-15 -0.28242954484648664 1106 16-17 -0.21934328049431429 1106 18-19 -0.2445780493142422 1106 20-21 -0.32353855773855855 1106 22-23 -0.27926844003326323 1106 24-25 -4.056608066938871 1106 26-27 -1.081181855793929 1106 28-29 -0.726768894555228 1106 30-31 -0.6427940172151665 1106 32-33 -5.545573746286799 1106 34-35 -1.2246378383374292 1106 36-37 -0.532035290346407 1106 38-39 -0.11992352077587753 1106 40-41 -0.7550959511411008 1106 42-43 -0.3535849305700509 1106 44-45 -0.3317957889944836 1106 46-47 -0.4121790976341799 1106 48-49 -0.3401267636990184 1106 50-51 -0.27225329925816055 1106 52-53 -0.17416078572519922 1106 54-55 -0.4221216307269984 1106 56-57 -0.35722406803485285 1106 58-59 -0.25676503986161237 1106 60-61 -0.21904048674050358 1106 62-63 -0.2881774046199297 1106 64-65 -0.1745546254819672 1106 66-67 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-0.9966999412916024 2216 106-107 -1.0028837747645127 2216 108-109 -0.9898756670835027 2216 110-111 -1.0207305399840791 2216 112-113 -1.0354377250908513 2216 114-115 -1.0256815514931432 2216 116-117 -0.8558344345602258 2216 118-119 -0.946720375428896 2216 120-121 -0.9364247511551191 2216 122-123 -0.8829469305663409 2216 124-125 -1.029498387551886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96985.0 3 16797.0 4 12061.0 5 10291.0 6 10234.0 7 10527.0 8 11261.0 9 12394.0 10 14149.0 11 16301.0 12 18326.0 13 19167.0 14 19606.0 15 19164.0 16 17943.0 17 15988.0 18 14714.0 19 13930.0 20 13382.0 21 13955.0 22 16877.0 23 23043.0 24 33171.0 25 47436.0 26 57978.0 27 56956.0 28 65084.0 29 81626.0 30 95555.0 31 117625.0 32 151228.0 33 198255.0 34 275125.0 35 389180.0 36 643380.0 37 992092.0 38 398696.0 39 1631.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.10838798765917 18.829804452751887 9.573240028771062 22.48856753081788 2 18.411638871954704 21.199505334018877 38.48041872570304 21.908437068323376 3 17.142987848918143 29.15389455934956 30.479467125596482 23.22365046613582 4 10.573984025252493 16.889220501340347 38.34195487031892 34.19484060308824 5 10.530747904255486 43.300964501194535 35.26390207651316 10.904385518036817 6 29.88163128003256 38.490855383005815 19.227054173038763 12.400459163922863 7 27.12953142178603 35.069200478757026 22.311951869703304 15.489316229753644 8 26.378691301490754 35.26277723065586 20.295212126531275 18.063319341322114 9 27.466093690289927 14.039122942368287 19.300906828601114 39.193876538740675 10-11 23.539900282082833 27.591781346083728 29.927831497679474 18.940486874153958 12-13 24.265338611937327 26.644887411136136 29.080303618353256 20.009470358573285 14-15 22.06869373499263 28.217900003294393 25.89562485664719 23.817781405065787 16-17 21.876424584700345 30.35005794994562 26.53099305806519 21.24252440728885 18-19 21.17072060984433 29.56331535724675 28.116924125002175 21.149039907906744 20-21 22.514407672422774 28.894149138647567 27.71085400780559 20.880589181124066 22-23 22.09731308132127 29.102508142479454 27.490921832197234 21.30925694400204 24-25 21.879383290218613 28.923241708180825 27.665416507594504 21.531958494006062 26-27 21.57622965482682 29.01326399561816 27.642571139344618 21.767935210210407 28-29 21.69158446889434 29.05486806193099 27.62384675007754 21.629700719097126 30-31 21.908447526332868 28.894448498470314 27.792626424485928 21.40447755071089 32-33 21.5293224773234 29.028895144693273 27.858183994823527 21.5835983831598 34-35 21.92034507569975 29.031949685659686 27.801738605505516 21.245966633135044 36-37 21.91191644145208 28.859137910887128 27.602328640085126 21.626617007575664 38-39 21.609898476494774 29.02371127728024 27.899738779015355 21.466651467209637 40-41 21.776546899711366 28.846479454190277 27.923134432819392 21.453839213278965 42-43 21.435904219016617 28.694936986129715 27.982265764407266 21.886893030446405 44-45 21.519047610759976 28.926856508355307 27.94274466298679 21.611351217897926 46-47 21.603178983799264 28.845747431651596 27.608812940696758 21.942260643852375 48-49 21.463370184674396 28.924436601123887 27.843435752314832 21.76875746188688 50-51 21.5372000339627 28.681015285825584 27.983089260613873 21.79869541959785 52-53 21.5914364376802 28.760602919222205 27.976043148984807 21.671917494112787 54-55 21.353758047346684 28.726667247629972 28.13813732804035 21.781437376982986 56-57 21.52408659091861 29.04051815219633 27.750731807556896 21.684663449328163 58-59 21.861823895735473 28.726348510191563 27.6753493685788 21.736478225494164 60-61 21.944073885254987 28.763291182199634 27.923065572256306 21.369569360289077 62-63 21.562046835423367 28.781626342206266 28.06964088698274 21.586685935387628 64-65 21.355000606652492 28.81502369922766 28.098353286564738 21.73162240755511 66-67 21.605194494573674 29.190154059895757 27.545206744379712 21.659444701150854 68-69 21.544104393097644 28.861864620640187 27.878934040757265 21.715096945504904 70-71 21.40757133540738 28.588363614846106 28.145546682061646 21.85851836768487 72-73 21.513296953548334 29.041058221869122 27.793751307617363 21.65189351696518 74-75 21.171427861067603 29.099885631883843 27.907647746202237 21.821038760846324 76-77 21.04617514387388 28.983921082002205 28.046798450261917 21.923105323861993 78-79 21.484566471250677 28.84646056472689 27.811430014170448 21.85754294985199 80-81 21.434573989214073 28.781669145889964 27.71022450251648 22.07353236237949 82-83 21.512940854134413 28.8585684070563 27.876060979978902 21.752429758830385 84-85 21.330359596932 28.78788085976469 27.842280420392278 22.039479122911025 86-87 21.110176138065174 28.90597835633396 28.064701516036212 21.919143989564656 88-89 21.364070556282375 28.74102395418904 28.109698579893955 21.78520690963463 90-91 21.493715912581564 28.73350971699261 28.07154266418057 21.701231706245252 92-93 21.524844850987083 28.709311377312503 27.920880156913015 21.844963614787396 94-95 21.232239224256084 28.572881259431483 28.126882955808785 22.067996560503648 96-97 21.29880074936072 28.652915955490858 28.142062780715026 21.906220514433397 98-99 21.09291758663672 28.681982145381145 28.01132444236482 22.21377582561731 100-101 21.07888456734712 28.740606427217035 28.103662801893094 22.07684620354275 102-103 21.131729972545248 28.707985746154502 27.963755004416257 22.196529276883993 104-105 21.104419188403263 28.667341604974123 28.0467447818252 22.181494424797418 106-107 21.06029386444991 28.643734573483826 28.22592966223427 22.070041899831992 108-109 21.17251639044381 28.719660166264532 27.92094497268767 22.186878470603986 110-111 21.087272657656886 28.94984750401099 27.86918586509991 22.093693973232213 112-113 21.151950135408374 28.779248612507047 27.960433530556543 22.108367721528037 114-115 20.965872623299283 28.89050192393588 27.846219885241354 22.29740556752348 116-117 20.94532773622378 28.83957190266917 28.01574361101083 22.199356750096218 118-119 20.983615320545493 28.574408572742755 27.93774319066148 22.504232916050274 120-121 20.901178109482917 28.757201343235504 27.959579118214844 22.382041429066728 122-123 21.07801315462169 28.713108836972474 27.89730051668482 22.31157749172102 124-125 21.189615617224593 28.457900386552044 27.6624820755837 22.690001920639666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1838.0 1 2287.5 2 2715.5 3 2479.0 4 1936.5 5 1483.0 6 1267.5 7 1156.5 8 1125.0 9 1129.0 10 1084.0 11 1055.0 12 1055.0 13 1080.5 14 1203.0 15 1385.5 16 1701.0 17 2005.5 18 2454.5 19 3098.5 20 4053.5 21 5785.0 22 8416.5 23 12361.5 24 17152.5 25 23173.5 26 31268.5 27 39236.0 28 45929.5 29 53307.0 30 64767.0 31 75224.0 32 83262.5 33 93849.0 34 105659.0 35 115149.0 36 125405.0 37 134496.0 38 136195.0 39 136563.5 40 137276.0 41 138696.0 42 139982.5 43 142421.0 44 149017.5 45 152934.5 46 150453.5 47 147863.5 48 146799.0 49 142381.0 50 136548.5 51 128568.5 52 119457.0 53 114628.0 54 108166.0 55 98355.5 56 88052.0 57 77707.5 58 70654.0 59 62750.5 60 51181.5 61 40374.0 62 32173.0 63 24724.0 64 18428.5 65 14552.5 66 11376.5 67 8808.5 68 6627.0 69 4442.5 70 3176.5 71 2432.0 72 1893.0 73 1567.5 74 1190.0 75 890.5 76 613.5 77 370.0 78 224.0 79 137.0 80 82.0 81 52.5 82 38.5 83 34.5 84 28.0 85 21.5 86 15.0 87 13.5 88 13.0 89 9.0 90 6.0 91 4.0 92 2.5 93 3.0 94 5.0 95 3.5 96 2.0 97 2.5 98 2.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.97800857410023E-4 2 1.243127679406322E-4 3 4.9725107176252874E-5 4 3.729383038218966E-4 5 8.453268219962989E-4 6 4.972510717625288E-4 7 7.707391612319196E-4 8 0.0013923030009350805 9 0.0018398289655213564 10-11 0.0016782223671985349 12-13 0.00223762982293138 14-15 0.0032445632432505007 16-17 0.0026727245107235923 18-19 0.0025608430195770233 20-21 0.0030829566449276785 22-23 0.09151905975789343 24-25 0.0022749236533135694 26-27 0.0014793219384935232 28-29 0.0013798717241410173 30-31 0.0011934025722300692 32-33 0.001752810027962914 34-35 9.696395899369311E-4 36-37 5.718387325269081E-4 38-39 9.820708667309945E-4 40-41 0.0012928527865825748 42-43 0.0015414783224638393 44-45 0.0014917532152875863 46-47 0.00181496641193323 48-49 1.7403787511688506E-4 50-51 0.0013301466169647646 52-53 0.0012058338490241324 54-55 0.0014295968313172702 56-57 0.0012804215097885117 58-59 8.826206523784887E-4 60-61 9.57208313142868E-4 62-63 0.0012555589562003853 64-65 0.001019364697113184 66-67 2.1133170549907473E-4 68-69 1.989004287050115E-4 70-71 5.469761789387817E-4 72-73 1.864691519109483E-4 74-75 3.232131966456437E-4 76-77 3.480757502337701E-4 78-79 2.361942590872012E-4 80-81 4.972510717625288E-4 82-83 1.4917532152875865E-4 84-85 3.1078191985158053E-4 86-87 3.1078191985158053E-4 88-89 4.723885181744024E-4 90-91 2.361942590872012E-4 92-93 2.983506430575173E-4 94-95 3.1078191985158053E-4 96-97 4.475259645862759E-4 98-99 4.102321342040863E-4 100-101 3.6050702702783335E-4 102-103 0.0010069334203191209 104-105 4.2266341099814946E-4 106-107 5.09682348556592E-4 108-109 7.085827772616035E-4 110-111 9.945021435250577E-4 112-113 5.09682348556592E-4 114-115 4.8481979496846556E-4 116-117 9.199144827606782E-4 118-119 0.0015663408760519656 120-121 8.328955452022357E-4 122-123 7.085827772616035E-4 124-125 3.853695806159598E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4022113.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.090329410220104 #Duplication Level Percentage of deduplicated Percentage of total 1 47.67054460271979 12.91410756452269 2 13.881826165412217 7.521264872728592 3 10.342814640875805 8.405707669505185 4 7.534756494548509 8.164761418524575 5 5.36602177564501 7.268364876231878 6 3.720602238740949 6.047540415113683 7 2.6205820222986724 4.969470116140027 8 1.8499943493901372 4.0093565065619705 9 1.2817291271703093 3.1250217842745815 >10 5.3137555542591 23.942629331338587 >50 0.2599022692518491 4.8716617438213 >100 0.15044876350844247 7.300888282308893 >500 0.006098049325632903 1.1035237389842287 >1k 9.239468537216983E-4 0.3557016799438464 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.243127679406322E-4 0.0 5 0.0 0.0 0.0 1.7403787511688509E-4 0.0 6 0.0 0.0 0.0 1.7403787511688509E-4 0.0 7 0.0 0.0 0.0 1.7403787511688509E-4 0.0 8 0.0 0.0 0.0 1.7403787511688509E-4 0.0 9 0.0 0.0 0.0 1.7403787511688509E-4 0.0 10-11 0.0 0.0 0.0 1.7403787511688509E-4 0.0 12-13 0.0 0.0 0.0 4.2266341099814946E-4 0.0 14-15 0.0 0.0 0.0 4.5995724138033915E-4 0.0 16-17 0.0 0.0 0.0 5.221136253506552E-4 0.0 18-19 0.0 0.0 0.0 5.469761789387817E-4 0.0 20-21 0.0 0.0 0.0 5.469761789387817E-4 0.0 22-23 0.0 0.0 0.0 5.469761789387817E-4 0.0 24-25 0.0 0.0 0.0 5.967012861150345E-4 0.0 26-27 0.0 0.0 0.0 7.583078844378565E-4 0.0 28-29 0.0 0.0 0.0 9.447770363488047E-4 0.0 30-31 0.0 0.0 0.0 0.0024116676980482647 0.0 32-33 0.0 0.0 0.0 0.007023671388645719 0.0 34-35 0.0 0.0 0.0 0.01351279787514672 0.0 36-37 0.0 0.0 0.0 0.024340439962775788 0.0 38-39 0.0 0.0 0.0 0.0445785585835107 0.0 40-41 0.0 0.0 0.0 0.06710403213435326 0.0 42-43 0.0 0.0 0.0 0.09391829617914763 0.0 44-45 0.0 0.0 0.0 0.12758219373747082 0.0 46-47 0.0 0.0 0.0 0.16771035522870692 0.0 48-49 0.0 0.0 0.0 0.2130347904198614 0.0 50-51 0.0 0.0 0.0 0.25676802218137584 0.0 52-53 0.0 0.0 0.0 0.3057845465803671 0.0 54-55 0.0 0.0 0.0 0.36730693543418597 0.0 56-57 0.0 0.0 0.0 0.44438085155737794 0.0 58-59 0.0 0.0 0.0 0.5331277365901952 0.0 60-61 0.0 0.0 0.0 0.6240998201691499 0.0 62-63 0.0 0.0 0.0 0.720454646599934 0.0 64-65 0.0 0.0 0.0 0.8292283185479871 0.0 66-67 0.0 0.0 0.0 0.9570964316517214 0.0 68-69 0.0 0.0 0.0 1.115135253534647 0.0 70-71 0.0 0.0 0.0 1.2841384615499365 0.0 72-73 0.0 0.0 0.0 1.4518239542250555 0.0 74-75 0.0 0.0 0.0 1.6333081641415843 0.0 76-77 0.0 0.0 0.0 1.8477103950087925 0.0 78-79 0.0 0.0 0.0 2.103036389082057 0.0 80-81 0.0 0.0 0.0 2.370370996538387 0.0 82-83 0.0 0.0 0.0 2.6458232277412392 0.0 84-85 0.0 0.0 0.0 2.9313572244240778 0.0 86-87 0.0 0.0 0.0 3.2520841657109085 0.0 88-89 0.0 0.0 0.0 3.6217779062895548 0.0 90-91 0.0 0.0 0.0 4.014494371490806 0.0 92-93 0.0 0.0 0.0 4.4198907390220015 0.0 94-95 0.0 0.0 0.0 4.839757112741487 0.0 96-97 0.0 0.0 0.0 5.292093981447065 0.0 98-99 0.0 0.0 0.0 5.780295083703516 0.0 100-101 0.0 0.0 0.0 6.316493345661845 0.0 102-103 0.0 0.0 0.0 6.852094906334059 0.0 104-105 0.0 0.0 0.0 7.421633852654065 0.0 106-107 0.0 0.0 0.0 8.02769589019503 0.0 108-109 0.0 0.0 0.0 8.66287197798769 0.0 110-111 0.0 2.486255358812644E-5 0.0 9.310355029806473 0.0 112-113 0.0 2.486255358812644E-5 0.0 9.98247687223109 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5545 0.0 56.864407 1 GTACATG 29710 0.0 54.44685 1 TACATGG 30625 0.0 51.926502 2 ACATGGG 30155 0.0 51.059498 3 CATGGGA 19635 0.0 46.69209 4 CATGGGG 10235 0.0 41.793877 4 GTATCAA 7580 0.0 41.04863 2 ATGGGAG 7690 0.0 38.682564 5 ATCAACG 8760 0.0 35.927185 4 TCAACGC 8780 0.0 35.913105 5 ATGGGAT 9240 0.0 35.863678 5 CAACGCA 8905 0.0 35.609417 6 ATGGGGG 6630 0.0 35.08608 5 AACGCAG 9255 0.0 34.327473 7 AGTACAT 16790 0.0 32.24459 2 GAGTACA 17455 0.0 32.072735 1 TATCAAC 10480 0.0 29.973967 3 ACGCAGA 11245 0.0 27.776802 8 CGCAGAG 11410 0.0 26.749403 9 TGGGGGG 6165 0.0 26.538185 6 >>END_MODULE