FastQCFastQC Report
Thu 26 May 2016
SRR936248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022113
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151080.37562345960941423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121770.30275131504261565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115630.287485707139506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.081.294821
GTATCAA151300.050.208392
GTACATG258600.046.2763561
ACATGGG261350.044.760943
TACATGG269000.044.351672
CATGGGA175250.040.1190874
ATCAACG203050.039.1708154
TCAACGC203850.039.0170945
CAACGCA207400.038.4368136
AACGCAG212350.037.48487
CATGGGG86850.037.426744
TATCAAC217800.036.764073
ACGCAGA246650.032.1031958
ATGGGAG67050.031.5201825
CGCAGAG250300.031.4924419
ATGGGGG57050.030.7840355
ATGGGAT82700.030.234515
AGTACAT166150.029.0234622
GAGTACA173200.028.9238341
CAGAGTA276350.026.0696969
Produced by FastQC (version 0.11.5)