FastQCFastQC Report
Thu 26 May 2016
SRR936247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355195
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG58910.17557846861359772No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG48040.14318094775415438No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40880.12184090641527542No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39220.11689335493168056No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36430.10857789189600009No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.059.2853431
GTACATG229700.052.5263521
TACATGG228950.051.425142
ACATGGG231400.049.7508933
CATGGGA155550.045.8980754
GTATCAA62650.040.168872
ATGGGAG58700.039.3257375
CATGGGG75250.038.7411774
ATGGGAT66050.037.832045
ATCAACG70200.035.680284
TCAACGC71700.035.265745
CAACGCA71650.035.2073176
GAGTACA133500.033.8244551
AACGCAG75300.033.4217077
TATCAAC83000.030.8229083
AGTACAT147450.029.0507952
ACGCAGA90050.027.4187478
ATGGGGG38450.027.0785225
CGCAGAG90600.026.9239589
CATGGGT30300.025.7224244
Produced by FastQC (version 0.11.5)