##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936247_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3355195 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.96193485028441 31.0 30.0 34.0 26.0 34.0 2 29.856378541336642 31.0 30.0 34.0 25.0 34.0 3 30.039742548495692 31.0 30.0 34.0 26.0 34.0 4 33.52249660600949 37.0 35.0 37.0 28.0 37.0 5 33.66830303454792 37.0 35.0 37.0 28.0 37.0 6 33.64549154371058 37.0 35.0 37.0 28.0 37.0 7 33.45913903662827 37.0 35.0 37.0 28.0 37.0 8 33.38625862282222 37.0 35.0 37.0 27.0 37.0 9 34.755340002592995 39.0 35.0 39.0 27.0 39.0 10-11 34.64822774831269 39.0 35.0 39.0 27.0 39.0 12-13 34.68183473687819 39.0 35.0 39.0 27.0 39.0 14-15 35.82322368744589 40.0 36.0 41.0 27.0 41.0 16-17 35.83967727658154 40.0 36.0 41.0 27.0 41.0 18-19 35.86208774750797 39.5 36.0 41.0 27.0 41.0 20-21 35.96423367345266 40.0 36.0 41.0 27.0 41.0 22-23 35.859364209829835 39.5 35.5 41.0 27.0 41.0 24-25 35.816832255651306 39.0 35.0 41.0 27.0 41.0 26-27 35.90497661089743 39.0 35.0 41.0 27.0 41.0 28-29 35.79677306386067 39.0 35.0 41.0 27.0 41.0 30-31 35.71680856105234 39.0 35.0 41.0 27.0 41.0 32-33 35.55401712866167 39.0 35.0 41.0 27.0 41.0 34-35 35.525080658501224 39.0 35.0 41.0 27.0 41.0 36-37 35.409704055949064 39.0 35.0 41.0 27.0 41.0 38-39 35.16019530906549 39.0 34.0 41.0 25.5 41.0 40-41 35.25228250518971 39.0 34.5 40.5 26.5 41.0 42-43 35.16248578696619 39.0 34.0 41.0 25.5 41.0 44-45 35.04027768877815 39.0 34.0 41.0 25.5 41.0 46-47 35.012200185086115 39.0 34.0 40.0 26.0 41.0 48-49 34.910671361873156 39.0 34.0 40.0 26.0 41.0 50-51 34.772835259947634 38.5 33.5 40.0 25.5 41.0 52-53 34.61557048100036 38.0 33.0 40.0 24.5 41.0 54-55 34.44853249960136 38.0 33.0 40.0 24.0 41.0 56-57 34.25041137698405 38.0 33.0 40.0 24.0 41.0 58-59 34.02393199203027 38.0 33.0 40.0 23.5 41.0 60-61 33.78165292926342 37.0 33.0 40.0 23.0 41.0 62-63 33.53756279441285 37.0 32.0 40.0 22.5 41.0 64-65 33.25297188389945 36.5 32.0 40.0 22.0 41.0 66-67 32.953207190640185 36.0 32.0 39.0 21.5 41.0 68-69 32.66121551802503 35.5 31.5 39.0 21.0 41.0 70-71 32.313585946569425 35.0 31.0 39.0 20.0 40.0 72-73 31.993030658426708 35.0 31.0 38.0 19.5 40.0 74-75 31.658202280344362 35.0 31.0 37.0 18.0 39.5 76-77 31.333192556617426 35.0 31.0 37.0 18.0 39.0 78-79 30.992776425811318 35.0 30.5 36.0 16.0 39.0 80-81 30.690461061130577 34.5 30.0 36.0 12.5 38.5 82-83 30.380480270148233 34.0 30.0 35.5 10.5 37.0 84-85 30.405978341050222 34.0 30.5 35.0 10.5 37.0 86-87 30.288398736884147 34.0 31.0 35.0 8.5 36.5 88-89 30.10419319890498 34.0 30.5 35.0 7.0 36.0 90-91 29.93100281801803 34.0 30.0 35.0 6.5 36.0 92-93 29.807113893529287 34.0 30.0 35.0 2.0 36.0 94-95 29.67008340796884 34.0 30.0 35.0 2.0 35.5 96-97 29.561284366482425 34.0 30.0 35.0 2.0 35.0 98-99 29.44527441773131 34.0 30.0 35.0 2.0 35.0 100-101 29.33082339476543 34.0 30.0 35.0 2.0 35.0 102-103 28.338538892672407 33.5 28.0 34.5 2.0 35.0 104-105 29.012044605455124 34.0 29.5 35.0 2.0 35.0 106-107 29.12901992283608 34.0 30.0 35.0 2.0 35.0 108-109 29.074618017730714 34.0 30.0 35.0 2.0 35.0 110-111 28.946080332141648 34.0 30.0 35.0 2.0 35.0 112-113 28.892028928273916 34.0 30.0 35.0 2.0 35.0 114-115 28.762341235010187 34.0 29.0 35.0 2.0 35.0 116-117 28.629702744549867 34.0 29.0 35.0 2.0 35.0 118-119 28.40523114155809 34.0 29.0 35.0 2.0 35.0 120-121 28.19722355928642 34.0 29.0 35.0 2.0 35.0 122-123 27.902148757374757 34.0 29.0 35.0 2.0 35.0 124-125 25.926368810158575 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5272003969481034 1101 2 0.6311675198345448 1101 3 0.5360919084744573 1101 4 0.5896997394732537 1101 5 0.5323855299464881 1101 6 0.46353539611931893 1101 7 0.5030553722308326 1101 8 0.4819960393324081 1101 9 0.6122022909682059 1101 10-11 0.6733979076638832 1101 12-13 0.6843456322102242 1101 14-15 0.7567628452203508 1101 16-17 0.7367590088754454 1101 18-19 0.7321698090722109 1101 20-21 0.6736658145796355 1101 22-23 0.6948101619214526 1101 24-25 0.81883901774723 1101 26-27 0.681020314658916 1101 28-29 0.7708304144726412 1101 30-31 0.7810298451194626 1101 32-33 0.8691829233724562 1101 34-35 0.7749919491199577 1101 36-37 0.7597121577520127 1101 38-39 0.9263882431379358 1101 40-41 0.8147545055292653 1101 42-43 0.9229256291919938 1101 44-45 0.8545587280442675 1101 46-47 0.7475501777936273 1101 48-49 0.8003144915516884 1101 50-51 0.7333883879248404 1101 52-53 0.6508046707809712 1101 54-55 0.7358520814171357 1101 56-57 0.764257339939455 1101 58-59 0.7300627799246442 1101 60-61 0.7523142129220659 1101 62-63 0.7281296272684799 1101 64-65 0.6964132745122242 1101 66-67 0.7130349124687214 1101 68-69 0.6270074170938855 1101 70-71 0.6113107585265425 1101 72-73 0.6710904771005914 1101 74-75 0.6425586332689264 1101 76-77 0.6657558002240869 1101 78-79 0.678704032714343 1101 80-81 0.5924887924124107 1101 82-83 0.5907355228674334 1101 84-85 0.40215359084054114 1101 86-87 0.5583634093392398 1101 88-89 0.49246450674046116 1101 90-91 0.5044897390604568 1101 92-93 0.35702399214475733 1101 94-95 0.5122005644213807 1101 96-97 0.524727201181868 1101 98-99 0.47676802875474067 1101 100-101 0.5559163151178623 1101 102-103 0.6625709135963653 1101 104-105 0.5787332607607993 1101 106-107 0.5627626009438096 1101 108-109 0.5257661172292565 1101 110-111 0.5975701779463627 1101 112-113 0.536676274846247 1101 114-115 0.5590775284595892 1101 116-117 0.5446434894874912 1101 118-119 0.5519335231628766 1101 120-121 0.5396223375359845 1101 122-123 0.487357066912228 1101 124-125 0.26983153877593224 1102 1 0.4289155171768364 1102 2 0.4667805663924547 1102 3 0.5601341435817524 1102 4 0.49907730409981355 1102 5 0.44610774105579054 1102 6 0.3886719187078427 1102 7 0.5240301827654079 1102 8 0.5268860872175054 1102 9 0.6368188250116589 1102 10-11 0.7108403835198942 1102 12-13 0.6311427753964267 1102 14-15 0.7724281072238242 1102 16-17 0.8062323629276023 1102 18-19 0.7574267097148777 1102 20-21 0.6982081125944362 1102 22-23 0.8228138473697442 1102 24-25 0.8444330205553783 1102 26-27 0.742603748267257 1102 28-29 0.7773821274713129 1102 30-31 0.761418726591522 1102 32-33 0.9024894156781542 1102 34-35 0.8473300155221963 1102 36-37 0.8623451768139461 1102 38-39 0.8806542663295858 1102 40-41 0.8657039283399897 1102 42-43 0.9268640565694568 1102 44-45 0.8537788339328998 1102 46-47 0.7620868377881038 1102 48-49 0.7579279553245897 1102 50-51 0.6452688543540432 1102 52-53 0.6945451967704983 1102 54-55 0.710595679747037 1102 56-57 0.7479033294789161 1102 58-59 0.8435685219530171 1102 60-61 0.7972338849928562 1102 62-63 0.7818516557907884 1102 64-65 0.7780091669109765 1102 66-67 0.7706045923234868 1102 68-69 0.7461488878639315 1102 70-71 0.6723837986473313 1102 72-73 0.6730334762494579 1102 74-75 0.5727924377654148 1102 76-77 0.6777671785725765 1102 78-79 0.7626846997822554 1102 80-81 0.7152837957085865 1102 82-83 0.6631041374786228 1102 84-85 0.6679944357437542 1102 86-87 0.7148334054169503 1102 88-89 0.7522644571832444 1102 90-91 0.8402576334361704 1102 92-93 0.8649279207811809 1102 94-95 0.8015000867826672 1102 96-97 0.8079767551743195 1102 98-99 0.828003037586182 1102 100-101 0.8361768941123167 1102 102-103 0.8528900253985974 1102 104-105 0.8736067316686302 1102 106-107 0.8192995110898664 1102 108-109 0.8209156308455867 1102 110-111 0.8504090546061107 1102 112-113 0.8133574094994991 1102 114-115 0.7751320555895767 1102 116-117 0.8519191081802013 1102 118-119 0.768339086729096 1102 120-121 0.845014963150291 1102 122-123 0.8750425017479664 1102 124-125 0.7206317355263572 1103 1 0.37428048995889895 1103 2 0.3893565089880049 1103 3 0.4762912615944117 1103 4 0.5084050213833677 1103 5 0.3915891112227001 1103 6 0.26235932236742343 1103 7 0.2908031649973921 1103 8 0.3661450198981413 1103 9 0.5117508769219157 1103 10-11 0.569901681776436 1103 12-13 0.4891623765969584 1103 14-15 0.5317136267342306 1103 16-17 0.46114410929919103 1103 18-19 0.5954427802939577 1103 20-21 0.41204171851396865 1103 22-23 0.5537531802192319 1103 24-25 0.6451677678837413 1103 26-27 0.4988240870283036 1103 28-29 0.6113289037995884 1103 30-31 0.6034337617995007 1103 32-33 0.6688946000355358 1103 34-35 0.5799626921678751 1103 36-37 0.5559523555854469 1103 38-39 0.6321390772151005 1103 40-41 0.621593085283557 1103 42-43 0.7271531596903955 1103 44-45 0.6210014047849697 1103 46-47 0.5964964500091838 1103 48-49 0.5250543718152727 1103 50-51 0.5083725802015877 1103 52-53 0.49968540650012727 1103 54-55 0.5520875965291623 1103 56-57 0.5099474818945993 1103 58-59 0.49859740993824886 1103 60-61 0.5741899266720552 1103 62-63 0.5406807397761142 1103 64-65 0.5379362860727213 1103 66-67 0.5852951181717501 1103 68-69 0.6155656089585264 1103 70-71 0.6364330076511919 1103 72-73 0.5684027209048566 1103 74-75 0.4997319426806044 1103 76-77 0.4319156587680588 1103 78-79 0.4548954076272018 1103 80-81 0.48023542679019826 1103 82-83 0.4657851175505563 1103 84-85 0.4918030296216678 1103 86-87 0.5332106174882192 1103 88-89 0.49595161078195105 1103 90-91 0.449812578657955 1103 92-93 0.494528966511961 1103 94-95 0.5578816255025103 1103 96-97 0.5719358083009425 1103 98-99 0.46693284252083345 1103 100-101 0.572021587850255 1103 102-103 0.5348705641130813 1103 104-105 0.6037815182684625 1103 106-107 0.5737760473418483 1103 108-109 0.52611342900002 1103 110-111 0.41023195196624584 1103 112-113 0.44702864255564023 1103 114-115 0.448710697651844 1103 116-117 0.4995357749758682 1103 118-119 0.451871193652746 1103 120-121 0.41678295607237814 1103 122-123 0.5635373496008036 1103 124-125 0.4546205458974555 1104 1 0.35711156877698613 1104 2 0.38136141449958316 1104 3 0.32106596248658903 1104 4 0.358291661576267 1104 5 0.19484812811408858 1104 6 0.28230558098408665 1104 7 0.10277798207118849 1104 8 0.11872095272318717 1104 9 0.2563600441299272 1104 10-11 0.3593574099185588 1104 12-13 0.4198147399044245 1104 14-15 0.4009157741734839 1104 16-17 0.43510845451518776 1104 18-19 0.40192849995735713 1104 20-21 0.3914074598299351 1104 22-23 0.44500172702568364 1104 24-25 0.4990569230303734 1104 26-27 0.3646796049088792 1104 28-29 0.4278849647523941 1104 30-31 0.4174030865440983 1104 32-33 0.5451084386190246 1104 34-35 0.5155672339766326 1104 36-37 0.4765917371044992 1104 38-39 0.5486662109552896 1104 40-41 0.4778937402234149 1104 42-43 0.6142399662960187 1104 44-45 0.5434552745235735 1104 46-47 0.4882729036717848 1104 48-49 0.42762711718059165 1104 50-51 0.4615685016418922 1104 52-53 0.5168939585542631 1104 54-55 0.5001622157366796 1104 56-57 0.4794137046462481 1104 58-59 0.3919773626707723 1104 60-61 0.42195094121056087 1104 62-63 0.3951925760735051 1104 64-65 0.4138651554161257 1104 66-67 0.48759432556907 1104 68-69 0.3753208792735805 1104 70-71 0.42694152086485815 1104 72-73 0.4533105866985423 1104 74-75 0.41763838220610694 1104 76-77 0.4150526672362851 1104 78-79 0.3953760610394532 1104 80-81 0.34997910996896664 1104 82-83 0.3494490799611363 1104 84-85 0.3761636348061401 1104 86-87 0.37159529268422986 1104 88-89 0.43182950588549573 1104 90-91 0.34178859019811014 1104 92-93 0.32114033635435035 1104 94-95 0.295021370532325 1104 96-97 0.22105944893986873 1104 98-99 0.2371871545296358 1104 100-101 0.30561863576763315 1104 102-103 0.3580318689283395 1104 104-105 0.25778906372060817 1104 106-107 0.31821923026303267 1104 108-109 0.3139085940483817 1104 110-111 0.2636023969828969 1104 112-113 0.24639740734150095 1104 114-115 0.26849203939287847 1104 116-117 0.31343014086196774 1104 118-119 0.3680762019017294 1104 120-121 0.3966920244580514 1104 122-123 0.4038232170914178 1104 124-125 0.3936890955611787 1105 1 0.1853278340426492 1105 2 0.2116581954889547 1105 3 0.24106838996565116 1105 4 0.1361568815810017 1105 5 0.12915982220549438 1105 6 0.12005992443769742 1105 7 0.034016964495080515 1105 8 0.021360908450589022 1105 9 0.022674256730851994 1105 10-11 0.14920859678242238 1105 12-13 0.153615290710988 1105 14-15 0.12584556873362374 1105 16-17 0.13847060332543037 1105 18-19 0.10345351310620288 1105 20-21 0.1175736792913824 1105 22-23 0.06157627953147937 1105 24-25 0.1978915344025296 1105 26-27 0.07847846876197906 1105 28-29 0.21011845500490267 1105 30-31 0.34913374184785084 1105 32-33 0.35749678180676625 1105 34-35 0.38272797571598716 1105 36-37 0.22163567014075625 1105 38-39 0.33309396888938636 1105 40-41 0.16033800934993536 1105 42-43 0.25338554062600593 1105 44-45 0.25496614905180337 1105 46-47 0.19567711216912187 1105 48-49 0.3331765257715631 1105 50-51 0.293672544405986 1105 52-53 0.26098199946306266 1105 54-55 0.2464324049933566 1105 56-57 0.22434794958466853 1105 58-59 0.2466686285144064 1105 60-61 0.30063693451187845 1105 62-63 0.2930585799516905 1105 64-65 0.34007338615956684 1105 66-67 0.3254970992528925 1105 68-69 0.377177467278365 1105 70-71 0.2579490980673569 1105 72-73 0.3479094457833867 1105 74-75 0.27762962594234253 1105 76-77 0.2118006580638827 1105 78-79 0.17413458972705342 1105 80-81 0.2170357988297269 1105 82-83 0.21170551928283032 1105 84-85 0.1714072388322876 1105 86-87 0.2435853270157189 1105 88-89 0.23003701849971492 1105 90-91 0.22682784166760328 1105 92-93 0.15521321996828874 1105 94-95 0.19876086229334788 1105 96-97 0.18398799652712228 1105 98-99 0.12994931143147426 1105 100-101 0.09506632648368551 1105 102-103 0.17861535614650492 1105 104-105 0.11713269072110677 1105 106-107 0.14781807489859133 1105 108-109 0.1799745860781634 1105 110-111 0.2167616417768201 1105 112-113 0.1750565249817626 1105 114-115 0.23317481989675315 1105 116-117 0.28134984910209937 1105 118-119 0.30881377985559766 1105 120-121 0.36546783448469355 1105 122-123 0.3878955227341514 1105 124-125 0.4349301044966154 1106 1 -0.1515996528848369 1106 2 -0.2343479942672353 1106 3 -0.08535383645657646 1106 4 -0.02001948709536805 1106 5 -0.06961955157387933 1106 6 -0.028592267964491214 1106 7 -0.23748259450447762 1106 8 -0.29589402130433484 1106 9 -0.21880852850193122 1106 10-11 -0.14692871185488343 1106 12-13 -0.1988267492310527 1106 14-15 -0.2926219153588647 1106 16-17 -0.21400195227212038 1106 18-19 -0.29322441482172223 1106 20-21 -0.2850162139235124 1106 22-23 -0.30864595319074795 1106 24-25 -4.047988064602066 1106 26-27 -1.169587232428718 1106 28-29 -0.7681886545522048 1106 30-31 -0.6020801906160784 1106 32-33 -5.430568250008264 1106 34-35 -1.169644173531161 1106 36-37 -0.4436556264005418 1106 38-39 -0.1466967921513671 1106 40-41 -0.657294133282214 1106 42-43 -0.22284670123123362 1106 44-45 -0.26581127172561736 1106 46-47 -0.3752739525319413 1106 48-49 -0.17981467784464655 1106 50-51 -0.1684000395416021 1106 52-53 -0.008671263940200902 1106 54-55 -0.26673559254964374 1106 56-57 -0.09039308078136798 1106 58-59 0.07598669220747212 1106 60-61 0.03555373505368209 1106 62-63 -0.014418543150434004 1106 64-65 0.03364639535757874 1106 66-67 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48-49 0.12214712489932822 2203 50-51 0.17956728818716527 2203 52-53 0.19565471903263898 2203 54-55 0.233580541125896 2203 56-57 0.1415171360721459 2203 58-59 0.0493576595166445 2203 60-61 0.09804093393118052 2203 62-63 0.13227893909652266 2203 64-65 0.08023701156102447 2203 66-67 0.06900393135963867 2203 68-69 0.01365042920107129 2203 70-71 0.05606834292106555 2203 72-73 0.12457243863722312 2203 74-75 0.1086469758196813 2203 76-77 0.011547231640872013 2203 78-79 -0.009133600090557081 2203 80-81 0.035697953224673284 2203 82-83 0.02859109630846035 2203 84-85 -0.021958232956386325 2203 86-87 0.004181240195226366 2203 88-89 0.09502961902590101 2203 90-91 0.12411936760353726 2203 92-93 0.04489165764975667 2203 94-95 0.10796705948229146 2203 96-97 0.09208708725844872 2203 98-99 0.08841082083525009 2203 100-101 0.12384887231746333 2203 102-103 0.10290114767540715 2203 104-105 0.13272891498960604 2203 106-107 0.1587134482646313 2203 108-109 0.1904538038523036 2203 110-111 0.1312718321632822 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46-47 0.8324415589626355 2204 48-49 0.8218106871528192 2204 50-51 0.7938685451107048 2204 52-53 0.6813704772643376 2204 54-55 0.7682721689116079 2204 56-57 0.7884489890490727 2204 58-59 0.6939708660055928 2204 60-61 0.7581972763018783 2204 62-63 0.6970556729749475 2204 64-65 0.6981622691757821 2204 66-67 0.6686701084142683 2204 68-69 0.6658843030782222 2204 70-71 0.6807820790398722 2204 72-73 0.7388097276720345 2204 74-75 0.6267046787964645 2204 76-77 0.7139038116931964 2204 78-79 0.7113637123486214 2204 80-81 0.6829933393512242 2204 82-83 0.6417376722074231 2204 84-85 0.6669212451747804 2204 86-87 0.739176412287037 2204 88-89 0.7946754082107788 2204 90-91 0.8177413370869111 2204 92-93 0.758820513837211 2204 94-95 0.792275220996661 2204 96-97 0.8433629965271265 2204 98-99 0.8099946778867277 2204 100-101 0.8185687930094083 2204 102-103 0.7571675936376963 2204 104-105 0.767247207986788 2204 106-107 0.7405065879359434 2204 108-109 0.7898605092635016 2204 110-111 0.8127381216922558 2204 112-113 0.7516531599359553 2204 114-115 0.7920014583745534 2204 116-117 0.7330764171077391 2204 118-119 0.8712433076921009 2204 120-121 0.8280228908624281 2204 122-123 0.9122568687524755 2204 124-125 0.9259515104162759 2205 1 0.2991449361246623 2205 2 0.35198238040550933 2205 3 0.3893431593480905 2205 4 0.32405180682101076 2205 5 0.45258833662936127 2205 6 0.47421060044643326 2205 7 0.48057853292922914 2205 8 0.47981659232262075 2205 9 0.4453294981457745 2205 10-11 0.5774539668267877 2205 12-13 0.5007812766744166 2205 14-15 0.6174030148054896 2205 16-17 0.5904043726609203 2205 18-19 0.4431383651119347 2205 20-21 0.5359766526735328 2205 22-23 0.559078509421397 2205 24-25 0.49024910018427903 2205 26-27 0.49456382366656015 2205 28-29 0.5325140316074908 2205 30-31 0.4619736533564307 2205 32-33 0.6484647007326672 2205 34-35 0.5567248920129586 2205 36-37 0.5687331456706488 2205 38-39 0.6421119664482475 2205 40-41 0.5741492433005888 2205 42-43 0.6383653982651367 2205 44-45 0.6332012498662962 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0.6028152207501307 2205 112-113 0.5400766673426247 2205 114-115 0.5299461740267439 2205 116-117 0.5628587803980167 2205 118-119 0.5735791719641341 2205 120-121 0.6278596408128898 2205 122-123 0.6504854518471284 2205 124-125 0.6185438611569438 2206 1 0.14508379055433096 2206 2 0.17825691165828061 2206 3 0.13577692570472166 2206 4 0.11853312493052925 2206 5 0.14915750899707092 2206 6 0.16051360243908874 2206 7 0.13211145905903976 2206 8 0.2610006262391664 2206 9 0.15293825164179253 2206 10-11 0.2753015877609073 2206 12-13 0.17111760391940578 2206 14-15 0.19885837812524443 2206 16-17 0.19529384528703275 2206 18-19 0.1820519979546873 2206 20-21 0.12425523723725718 2206 22-23 0.21961467879125252 2206 24-25 0.32186721679901353 2206 26-27 0.244996945514238 2206 28-29 0.16475788364242305 2206 30-31 0.1377166125395064 2206 32-33 0.32446561132330487 2206 34-35 0.34135985751102993 2206 36-37 0.37987213787150154 2206 38-39 0.39364075352968797 2206 40-41 0.26161605700202273 2206 42-43 0.39303321006852343 2206 44-45 0.4277686010527262 2206 46-47 0.24381353363569502 2206 48-49 0.277155677980673 2206 50-51 0.2875663970546043 2206 52-53 0.28710824281259306 2206 54-55 0.2883976201217351 2206 56-57 0.3455795884928321 2206 58-59 0.41168641130413874 2206 60-61 0.3096707362699149 2206 62-63 0.37302836366670533 2206 64-65 0.3751394571750666 2206 66-67 0.3349928198173089 2206 68-69 0.2638013395892642 2206 70-71 0.2975049620507093 2206 72-73 0.2618754416196083 2206 74-75 0.329616093673085 2206 76-77 0.3250228128175259 2206 78-79 0.23377850020588653 2206 80-81 0.19848242154543527 2206 82-83 0.22754304530642244 2206 84-85 0.20151870656303217 2206 86-87 0.2611902888477786 2206 88-89 0.22402773675093357 2206 90-91 0.18669512133449828 2206 92-93 0.2504176497624151 2206 94-95 0.29787244568451143 2206 96-97 0.23840116446605464 2206 98-99 0.26659270143841596 2206 100-101 0.2957834210939083 2206 102-103 0.28868721018305266 2206 104-105 0.3114768961340175 2206 106-107 0.2879435664553789 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116-117 -0.9253679514644269 2216 118-119 -1.0294911707844214 2216 120-121 -1.0156682510970079 2216 122-123 -1.0115750823127847 2216 124-125 -0.971982668700889 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87312.0 3 15854.0 4 10252.0 5 8861.0 6 9364.0 7 9931.0 8 10437.0 9 11687.0 10 13527.0 11 15311.0 12 16817.0 13 17695.0 14 18131.0 15 16933.0 16 15672.0 17 14259.0 18 13114.0 19 12644.0 20 12460.0 21 13092.0 22 15660.0 23 20519.0 24 29120.0 25 41174.0 26 50669.0 27 51484.0 28 58699.0 29 71752.0 30 85516.0 31 104776.0 32 133407.0 33 174632.0 34 237508.0 35 342698.0 36 561402.0 37 758204.0 38 273592.0 39 1030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.67693286659305 17.859015225688 9.36297104332548 23.10108086439347 2 19.016085820449565 20.478565899825078 37.03508980561762 23.470258474107734 3 17.209352785368832 27.755065058850565 30.610310643828836 24.42527151195177 4 10.777405485120646 16.153798925007436 37.30857246066239 35.76022312920953 5 10.705812644851168 42.69076839073334 35.13472350713884 11.468695457276654 6 31.372759990164447 37.70232551208208 18.31138465206733 12.613529845686141 7 27.98419038302943 34.55786035474176 21.432426882309795 16.025522379919007 8 27.04082910004781 34.237424429743356 19.622187463116468 19.099559007092367 9 27.24219062487519 13.78970258713959 18.665305171119844 40.30280161686537 10-11 23.862336799154733 26.88509736538957 29.41576295510328 19.836802880352415 12-13 24.660955709757253 26.012319345646716 28.375640940237922 20.951084004358105 14-15 22.282387746878182 27.62819603373495 25.211499164256402 24.877917055130464 16-17 22.355261430364642 29.84787828832412 26.06806126595868 21.728799015352553 18-19 21.866708076144757 28.542123587918667 27.600097701729258 21.99107063420732 20-21 22.96488099806488 27.845968479259515 27.2784133373372 21.91073718533841 22-23 22.59408010144869 28.397847228260893 26.95612317329043 22.05194949699999 24-25 22.06730135870035 28.387153596386177 27.405861731241643 22.139683313671828 26-27 21.975514590892008 28.30584467239976 27.215905382217915 22.50273535449032 28-29 22.294636700554417 28.43809605276433 27.111849545720148 22.155417700961106 30-31 22.000798769656292 28.08784081832163 27.263215465134298 22.64814494688778 32-33 21.533871562640876 28.47370636821889 27.21733460998245 22.775087459157785 34-35 22.422184446262868 28.402969573893834 27.237824468360166 21.937021511483128 36-37 22.605238827865467 27.8780589286172 26.93734877851488 22.579353465002452 38-39 22.03919421512543 28.277116748557855 27.132805897536738 22.550883138779973 40-41 22.376119938583976 28.019965125260406 27.462493858323196 22.141421077832423 42-43 21.963902960537833 28.01075540426577 27.46037770883899 22.5649639263574 44-45 22.154091471320207 28.145403335171302 27.666810723812645 22.033694469695842 46-47 22.601492814455675 28.16571832046303 26.906456036545727 22.32633282853557 48-49 21.95921769943261 28.31698200484564 27.077197921662048 22.6466023740597 50-51 22.308783969963798 28.29030224119537 27.21419584182711 22.18671794701372 52-53 22.43212491831593 28.067755489504577 27.26959504821316 22.230524543966332 54-55 21.745157514707998 28.51389223780657 27.5444116159441 22.19653863154133 56-57 22.07162956302116 28.698946783617192 27.03094435988127 22.198479293480382 58-59 22.122657831746878 28.048764131545006 27.278088061681505 22.550489975026608 60-61 21.872254701962063 28.282320247105325 27.478605023860947 22.366820027071665 62-63 21.84840099739088 28.1730585242883 27.84681418498227 22.131726293338545 64-65 21.688004176249535 28.50200891849235 27.42545929579885 22.38452760945927 66-67 21.714460624880875 28.794013799841352 27.19319226504303 22.298333310234746 68-69 22.327740355649063 28.06632829531685 27.47285958065856 22.133071768375526 70-71 22.03899112236802 28.055921987950548 27.45060315798823 22.4544837316932 72-73 22.016458084340975 28.319320217334937 27.25242087631401 22.41180082201008 74-75 21.81742911604414 28.713189989999062 27.29098802167213 22.178392872284668 76-77 21.895773823432634 28.551995956108517 27.359110558467137 22.193119661991712 78-79 22.18159854899273 28.528935723841283 27.25040768970667 22.03905803745932 80-81 22.13373724566779 28.46210944945336 27.066984562841345 22.33716874203751 82-83 21.829273726565965 28.668872503326565 27.379943815394032 22.121909954713438 84-85 21.80014568500565 28.65504544609178 27.48189071001769 22.062918158884877 86-87 21.8794227262316 28.756630224060704 27.187074213057432 22.176872836650265 88-89 21.83043369020408 28.8085329473832 27.15528482351037 22.20574853890235 90-91 21.80703760967159 28.731583555315467 27.26752230687555 22.1938565281374 92-93 21.841388775879718 28.506714234833545 27.44496950242684 22.20692748685989 94-95 21.54591750590243 28.394316797089164 27.811159770450843 22.248605926557563 96-97 21.901266249103998 28.128642682892302 27.69614790242565 22.273943165578054 98-99 21.584214395427253 28.57465172918262 27.382123809323694 22.459010066066433 100-101 21.605255636137446 28.38055734135198 27.581419650039535 22.432767372471034 102-103 21.46579225454698 28.05797980041837 27.816352591531547 22.659875353503107 104-105 21.187629454698325 28.37238663385968 27.528378138934144 22.911605772507848 106-107 21.42961884917684 28.56704302986933 27.251197476380558 22.752140644573274 108-109 21.39126018501574 28.50291817950425 27.18626515346149 22.919556482018518 110-111 21.440238939032962 28.44740445739562 27.43919785775098 22.673158745820437 112-113 21.45644834520924 28.490298217757154 27.573805597758096 22.479447839275508 114-115 21.351451962098057 28.866390040009726 27.224587384775322 22.557570613116894 116-117 21.331741164348827 28.727369077854885 27.497297828143807 22.443591929652477 118-119 21.30241471885406 28.716041968286092 27.334808179359953 22.64673513349989 120-121 21.306940448954627 28.824468611445255 27.322205836243267 22.546385103356847 122-123 21.376043853430662 28.744210624985005 27.228299315997234 22.651446205587096 124-125 21.36378152564143 28.595646947091304 27.17150810290519 22.86906342436208 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1175.0 1 1453.0 2 1923.5 3 1888.0 4 1510.5 5 1269.0 6 1115.0 7 967.5 8 869.5 9 867.0 10 858.5 11 831.5 12 829.5 13 886.0 14 972.5 15 1099.0 16 1289.0 17 1492.5 18 1880.0 19 2426.5 20 3275.0 21 4828.0 22 7024.5 23 9583.5 24 12603.0 25 17020.5 26 23729.5 27 30549.0 28 35274.5 29 40653.0 30 47945.5 31 56628.0 32 69571.5 33 77398.0 34 79906.5 35 85361.5 36 92367.0 37 99150.0 38 101526.5 39 101976.0 40 103322.0 41 107163.5 42 109550.0 43 112253.5 44 115169.0 45 118003.5 46 120084.0 47 117498.0 48 114229.0 49 110181.5 50 108342.5 51 106476.5 52 103655.5 53 103513.5 54 102628.0 55 97923.0 56 91547.5 57 83765.0 58 77965.5 59 70941.0 60 57813.5 61 47591.5 62 40832.5 63 30213.5 64 20406.5 65 15698.0 66 11711.5 67 8730.5 68 6923.5 69 5283.5 70 3940.0 71 2709.5 72 2079.5 73 1690.5 74 1246.0 75 896.0 76 560.5 77 356.0 78 269.0 79 196.5 80 119.5 81 69.0 82 58.5 83 44.5 84 28.0 85 27.5 86 18.0 87 12.0 88 12.5 89 11.0 90 8.5 91 4.5 92 4.5 93 5.0 94 2.5 95 2.0 96 3.5 97 5.0 98 3.5 99 1.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.980452700960749E-4 2 1.1921810803842996E-4 3 5.960905401921498E-5 4 2.682407430864674E-4 5 8.047222292594022E-4 6 5.960905401921498E-4 7 6.556995942113647E-4 8 0.0011623765533746921 9 0.001818076147586057 10-11 0.0014902263504803744 12-13 0.0019521965191292906 14-15 0.0029506481739511414 16-17 0.0025482870593214403 18-19 0.0025482870593214403 20-21 0.0030549640184847674 22-23 0.08817669315792376 24-25 0.0022949485797397764 26-27 0.0016094444585188043 28-29 0.0014753240869755707 30-31 0.0010431584453362622 32-33 0.0017137603030524307 34-35 9.984516548218508E-4 36-37 5.066769591633273E-4 38-39 9.38842600802636E-4 40-41 0.0013263014519275332 42-43 0.0013263014519275332 44-45 0.0013412037154323369 46-47 0.0016690535125380194 48-49 4.1726337813450485E-4 50-51 0.001296496924917926 52-53 0.0012666923979083183 54-55 0.0014306172964611596 56-57 0.0013113991884227294 58-59 7.153086482305798E-4 60-61 7.898199657545983E-4 62-63 0.001221985607393907 64-65 9.38842600802636E-4 66-67 3.427520606104861E-4 68-69 1.6392489855284118E-4 70-71 6.258950672017573E-4 72-73 1.4902263504803744E-4 74-75 3.1294753360087865E-4 76-77 3.2784979710568236E-4 78-79 2.5333847958166364E-4 80-81 3.427520606104861E-4 82-83 1.6392489855284118E-4 84-85 3.2784979710568236E-4 86-87 3.1294753360087865E-4 88-89 3.576543241152899E-4 90-91 2.2353395257205616E-4 92-93 2.980452700960749E-4 94-95 2.8314300659127117E-4 96-97 2.980452700960749E-4 98-99 3.427520606104861E-4 100-101 4.7687243215371986E-4 102-103 7.898199657545983E-4 104-105 4.619701686489161E-4 106-107 4.1726337813450485E-4 108-109 6.258950672017573E-4 110-111 8.792335467834209E-4 112-113 3.725565876200936E-4 114-115 3.2784979710568236E-4 116-117 7.00406384725776E-4 118-119 0.0012666923979083183 120-121 6.855041212209723E-4 122-123 5.513837496777386E-4 124-125 2.8314300659127117E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3355195.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.3394987197283 #Duplication Level Percentage of deduplicated Percentage of total 1 47.95672650514611 13.111128628898022 2 13.936611928538161 7.620399679552385 3 10.054042537923419 8.246174492809534 4 7.639540600878608 8.354448419081322 5 5.473864336137304 7.482635350489611 6 3.815795842511037 6.259316733064503 7 2.5667948650178944 4.912241944817333 8 1.79113644299306 3.9175017992053975 9 1.2890455884921272 3.1717674194626726 >10 5.130803751162178 23.05394815304494 >50 0.21442676822060058 4.015379554189866 >100 0.11966091690575958 5.977445614221282 >500 0.007589944849517919 1.42511022651271 >1k 0.003849972023635392 2.275340586364883 >5k 1.0999920067529691E-4 0.17716139828542854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5891 0.17557846861359772 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 4804 0.14318094775415438 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4088 0.12184090641527542 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3922 0.11689335493168056 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3643 0.10857789189600009 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.9804527009607488E-5 0.0 4 0.0 0.0 0.0 5.9609054019214975E-5 0.0 5 0.0 0.0 0.0 1.7882716205764495E-4 0.0 6 0.0 0.0 0.0 1.7882716205764495E-4 0.0 7 0.0 8.941358102882247E-5 0.0 1.7882716205764495E-4 0.0 8 0.0 8.941358102882247E-5 0.0 1.7882716205764495E-4 0.0 9 0.0 8.941358102882247E-5 0.0 4.1726337813450485E-4 0.0 10-11 0.0 8.941358102882247E-5 0.0 4.1726337813450485E-4 2.9804527009607488E-5 12-13 0.0 8.941358102882247E-5 0.0 4.1726337813450485E-4 2.9804527009607488E-5 14-15 0.0 8.941358102882247E-5 0.0 4.1726337813450485E-4 2.9804527009607488E-5 16-17 0.0 8.941358102882247E-5 0.0 5.364814861729348E-4 2.9804527009607488E-5 18-19 0.0 8.941358102882247E-5 0.0 6.556995942113647E-4 2.9804527009607488E-5 20-21 0.0 8.941358102882247E-5 0.0 9.239403372978322E-4 2.9804527009607488E-5 22-23 0.0 8.941358102882247E-5 0.0 9.835493913170471E-4 2.9804527009607488E-5 24-25 0.0 8.941358102882247E-5 0.0 0.001102767499355477 4.470679051441123E-5 26-27 0.0 8.941358102882247E-5 0.0 0.001266692397908318 5.9609054019214975E-5 28-29 0.0 8.941358102882247E-5 0.0 0.0014902263504803746 5.9609054019214975E-5 30-31 0.0 8.941358102882247E-5 0.0 0.002965550437455945 5.9609054019214975E-5 32-33 0.0 8.941358102882247E-5 0.0 0.00831546303568049 5.9609054019214975E-5 34-35 0.0 8.941358102882247E-5 0.0 0.015215211038404623 5.9609054019214975E-5 36-37 0.0 8.941358102882247E-5 0.0 0.026660149410093897 5.9609054019214975E-5 38-39 0.0 8.941358102882247E-5 0.0 0.046614280243026114 1.0431584453362621E-4 40-41 0.0 8.941358102882247E-5 0.0 0.06774568989283783 1.4902263504803744E-4 42-43 0.0 8.941358102882247E-5 0.0 0.09518075700518153 1.4902263504803744E-4 44-45 0.0 8.941358102882247E-5 0.0 0.12924733137716288 1.7882716205764492E-4 46-47 0.0 8.941358102882247E-5 0.0 0.17095876692710854 2.0863168906725242E-4 48-49 0.0 8.941358102882247E-5 0.0 0.21511417369184205 2.0863168906725242E-4 50-51 0.0 8.941358102882247E-5 0.0 0.2619966946779546 2.0863168906725242E-4 52-53 0.0 8.941358102882247E-5 0.0 0.31056317144011003 2.0863168906725242E-4 54-55 0.0 8.941358102882247E-5 0.0 0.3728397306266849 2.0863168906725242E-4 56-57 0.0 8.941358102882247E-5 0.0 0.4517174113576111 2.0863168906725242E-4 58-59 0.0 8.941358102882247E-5 0.0 0.5375097423547662 2.0863168906725242E-4 60-61 0.0 8.941358102882247E-5 0.0 0.6249711268644594 2.682407430864674E-4 62-63 0.0 8.941358102882247E-5 0.0 0.7191087254243047 2.682407430864674E-4 64-65 0.0 8.941358102882247E-5 0.0 0.8271948426246463 2.980452700960749E-4 66-67 0.0 8.941358102882247E-5 0.0 0.961464236802928 2.980452700960749E-4 68-69 0.0 8.941358102882247E-5 0.0 1.11850428961655 2.980452700960749E-4 70-71 0.0 8.941358102882247E-5 0.0 1.2753953197951238 2.980452700960749E-4 72-73 0.0 8.941358102882247E-5 0.0 1.4425242050014977 2.980452700960749E-4 74-75 0.0 8.941358102882247E-5 0.0 1.6225584504030317 2.980452700960749E-4 76-77 0.0 8.941358102882247E-5 0.0 1.8320693730170676 2.980452700960749E-4 78-79 0.0 1.1921810803842995E-4 0.0 2.082248572735713 2.980452700960749E-4 80-81 0.0 1.1921810803842995E-4 0.0 2.3400726336323223 2.980452700960749E-4 82-83 0.0 1.1921810803842995E-4 0.0 2.6150342975594563 2.980452700960749E-4 84-85 0.0 1.1921810803842995E-4 0.0 2.910277942116628 2.980452700960749E-4 86-87 0.0 1.1921810803842995E-4 0.0 3.2277259592959573 2.980452700960749E-4 88-89 0.0 1.1921810803842995E-4 0.0 3.582444537500801 2.980452700960749E-4 90-91 0.0 1.1921810803842995E-4 0.0 3.9684280645387227 2.980452700960749E-4 92-93 0.0 1.1921810803842995E-4 0.0 4.362488618396248 2.980452700960749E-4 94-95 0.0 1.1921810803842995E-4 0.0 4.768247449105044 2.980452700960749E-4 96-97 0.0 1.4902263504803744E-4 0.0 5.220560951002848 3.427520606104861E-4 98-99 0.0 1.4902263504803744E-4 0.0 5.707864967609931 3.8745885112489737E-4 100-101 0.0 1.4902263504803744E-4 0.0 6.237178465037054 3.8745885112489737E-4 102-103 0.0 1.4902263504803744E-4 0.0 6.770873227934591 4.023611146297011E-4 104-105 0.0 1.4902263504803744E-4 0.0 7.335788232874691 4.1726337813450485E-4 106-107 0.0 1.4902263504803744E-4 0.0 7.9103748068294095 4.6197016864891604E-4 108-109 0.0 1.4902263504803744E-4 0.0 8.52074171545916 5.066769591633273E-4 110-111 0.0 1.4902263504803744E-4 0.0 9.170569817849632 5.066769591633273E-4 112-113 0.0 1.4902263504803744E-4 0.0 9.836805312358894 5.066769591633273E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4285 0.0 59.285343 1 GTACATG 22970 0.0 52.526352 1 TACATGG 22895 0.0 51.42514 2 ACATGGG 23140 0.0 49.750893 3 CATGGGA 15555 0.0 45.898075 4 GTATCAA 6265 0.0 40.16887 2 ATGGGAG 5870 0.0 39.325737 5 CATGGGG 7525 0.0 38.741177 4 ATGGGAT 6605 0.0 37.83204 5 ATCAACG 7020 0.0 35.68028 4 TCAACGC 7170 0.0 35.26574 5 CAACGCA 7165 0.0 35.207317 6 GAGTACA 13350 0.0 33.824455 1 AACGCAG 7530 0.0 33.421707 7 TATCAAC 8300 0.0 30.822908 3 AGTACAT 14745 0.0 29.050795 2 ACGCAGA 9005 0.0 27.418747 8 ATGGGGG 3845 0.0 27.078522 5 CGCAGAG 9060 0.0 26.923958 9 CATGGGT 3030 0.0 25.722424 4 >>END_MODULE