FastQCFastQC Report
Thu 26 May 2016
SRR936247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3355195
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125590.37431505471366044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97970.29199495111312457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90980.2711615867334089No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG62330.18577161685088348No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG43990.13111011431526334No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35500.1058060708841066No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34820.10377936304745328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.084.771451
GTATCAA123500.053.456682
GTACATG200750.044.639411
TACATGG201650.044.0756762
ACATGGG202750.043.3374063
ATCAACG173100.039.4804654
CAACGCA174150.039.2531936
TCAACGC174200.039.231165
CATGGGA136400.039.104894
AACGCAG179900.038.0316477
TATCAAC181650.037.7204933
ATGGGAT57500.034.889885
CATGGGG69100.033.771124
ACGCAGA213150.032.0431378
CGCAGAG216300.031.4389619
GAGTACA127950.029.5239931
ATGGGAG52350.028.9975265
CAGAGTA238850.026.6026259
GTGGTAT24950.025.5528531
GCAGAGT251650.025.5095628
Produced by FastQC (version 0.11.5)