FastQCFastQC Report
Thu 26 May 2016
SRR936246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720255
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62450.8670540294756718No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23660.32849476921368126No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12780.17743715767332402No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11290.15675003991641848No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9670.13425800584515207No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8710.12092939306217938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG5850.055.9346922
GGTATCA24150.053.212171
GTACATG113800.053.01151
TACATGG115100.050.8103942
ACATGGG117150.046.620273
GTCGGGA4500.046.2732432
CGGGAGT4450.045.456224
CATGGGA97700.044.3313754
CATGGGG17800.043.1165584
ATGGGAG27450.042.6970945
ATGGGAT36800.042.5189325
CGCCGGT600.001308817439.662787
TCGGGAG5300.039.2886053
TGGGATA9900.039.061836
CATTCCG2450.038.8533369
GTATCAA33800.038.0199432
GAGAACA15100.037.824111
ACCTGGG8400.036.121463
GGAACGC3150.035.8853728
CCGGGTC2500.035.69659
Produced by FastQC (version 0.11.5)