##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936246_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 720255 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.686347196479026 31.0 26.0 31.0 10.0 34.0 2 26.876764479246933 31.0 26.0 33.0 10.0 34.0 3 27.300905929150094 31.0 26.0 34.0 10.0 34.0 4 29.786664445231203 35.0 28.0 37.0 7.0 37.0 5 30.94758245343663 35.0 31.0 37.0 10.0 37.0 6 30.348258602855932 35.0 30.0 37.0 8.0 37.0 7 30.421685375318464 35.0 31.0 37.0 8.0 37.0 8 30.048914620516346 35.0 30.0 37.0 8.0 37.0 9 30.798247842777904 37.0 29.0 39.0 8.0 39.0 10-11 31.055702494255506 37.0 30.0 39.0 7.5 39.0 12-13 31.03238783486404 37.0 30.0 39.0 7.5 39.0 14-15 31.89510589999375 38.0 30.0 40.0 7.5 41.0 16-17 32.010481704396355 38.0 30.0 40.0 8.0 41.0 18-19 32.106261671213666 38.0 30.0 40.0 8.0 41.0 20-21 32.31352645937897 38.0 30.0 40.0 8.0 41.0 22-23 32.319662480649214 38.0 30.0 40.0 8.0 41.0 24-25 32.41866838827915 38.0 30.0 40.0 8.0 41.0 26-27 32.574927629797784 38.0 30.0 40.0 7.5 41.0 28-29 32.47183358671582 38.0 30.0 40.0 2.0 41.0 30-31 32.37922610742029 38.0 30.0 40.0 2.0 41.0 32-33 32.20514331729734 38.0 30.0 40.0 2.0 41.0 34-35 32.10907109287683 38.0 30.0 40.0 2.0 41.0 36-37 31.941468646521024 38.0 30.0 40.0 2.0 41.0 38-39 31.671603806985026 37.0 29.0 40.0 2.0 41.0 40-41 31.771532304531036 37.5 29.5 40.0 2.0 41.0 42-43 31.654424474665223 38.0 29.0 40.0 2.0 41.0 44-45 31.570527104983654 37.0 29.0 40.0 2.0 41.0 46-47 31.56534630096285 37.0 29.0 40.0 2.0 41.0 48-49 31.47213278630485 37.0 29.0 40.0 2.0 41.0 50-51 31.379110870455605 37.0 29.0 40.0 2.0 41.0 52-53 31.248535588090327 37.0 29.0 40.0 2.0 41.0 54-55 31.08176340323913 37.0 29.0 40.0 2.0 41.0 56-57 30.91324947414457 36.0 29.0 40.0 2.0 41.0 58-59 30.696194403371027 36.0 29.0 40.0 2.0 41.0 60-61 30.455004130481566 36.0 29.0 40.0 2.0 41.0 62-63 30.207668811740284 35.0 28.5 39.5 2.0 41.0 64-65 29.913210599023955 35.0 28.0 39.0 2.0 41.0 66-67 29.612347015987392 35.0 28.0 39.0 2.0 41.0 68-69 29.303410597635562 35.0 28.0 39.0 2.0 40.0 70-71 28.942833440934116 34.0 28.0 38.0 2.0 40.0 72-73 28.582925491666145 34.0 27.0 37.5 2.0 40.0 74-75 28.209197437018833 34.0 26.0 37.0 2.0 39.5 76-77 27.850592498490116 34.0 26.0 36.5 2.0 39.0 78-79 27.474749220762092 34.0 26.0 36.0 2.0 39.0 80-81 27.133966442440524 34.0 25.5 36.0 2.0 37.5 82-83 26.80743972620808 33.5 25.0 35.0 2.0 37.0 84-85 26.745691456498044 34.0 25.0 35.0 2.0 37.0 86-87 26.577215014126942 34.0 25.0 35.0 2.0 36.5 88-89 26.354646618211603 34.0 25.0 35.0 2.0 36.0 90-91 26.135930330230266 33.5 24.0 35.0 2.0 36.0 92-93 25.97208488660266 33.0 23.5 35.0 2.0 35.5 94-95 25.823023095986837 33.0 23.0 35.0 2.0 35.0 96-97 25.67937397171835 33.0 21.0 35.0 2.0 35.0 98-99 25.53803930552374 33.0 20.0 35.0 2.0 35.0 100-101 25.39704271403878 33.0 19.5 35.0 2.0 35.0 102-103 24.514727422926605 31.5 18.0 34.0 2.0 35.0 104-105 25.052969434436413 33.0 18.0 35.0 2.0 35.0 106-107 25.12154028781473 33.0 17.5 35.0 2.0 35.0 108-109 25.0316894710901 33.0 16.0 35.0 2.0 35.0 110-111 24.8798029864423 33.0 8.5 35.0 2.0 35.0 112-113 24.803988865054738 33.0 4.5 35.0 2.0 35.0 114-115 24.651238103171792 33.0 2.0 35.0 2.0 35.0 116-117 24.493835516587875 33.0 2.0 35.0 2.0 35.0 118-119 24.27681168475054 33.0 2.0 35.0 2.0 35.0 120-121 24.037159061721198 33.0 2.0 35.0 2.0 35.0 122-123 23.715775662786097 33.0 2.0 35.0 2.0 35.0 124-125 21.96620988399942 29.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6946297818717184 1101 2 0.5584994673654613 1101 3 0.6844680196677579 1101 4 0.8500723394862035 1101 5 0.6285698692195751 1101 6 0.5647739685937161 1101 7 0.3626545055529071 1101 8 0.48373655581823627 1101 9 0.8215626170628774 1101 10-11 0.5679380728056032 1101 12-13 0.5485933971152654 1101 14-15 0.629660294027321 1101 16-17 0.6489856609911655 1101 18-19 0.43230373918427745 1101 20-21 0.25528416335107806 1101 22-23 0.34111503503726937 1101 24-25 0.5935703650434618 1101 26-27 0.7279302144747462 1101 28-29 0.5777656578158954 1101 30-31 0.6610396502754128 1101 32-33 0.7311548274290729 1101 34-35 0.8180238691584805 1101 36-37 0.7335715481951617 1101 38-39 0.8324603666636818 1101 40-41 0.755047981899029 1101 42-43 0.8765039433282027 1101 44-45 0.9293405902019778 1101 46-47 0.592100991016224 1101 48-49 0.6443207201860268 1101 50-51 0.6615665972887541 1101 52-53 0.7398128764817429 1101 54-55 0.7244350000633872 1101 56-57 0.5222445649377683 1101 58-59 0.4691041022062734 1101 60-61 0.5877331803584163 1101 62-63 0.6521458100563144 1101 64-65 0.60471835956292 1101 66-67 0.5848390023375778 1101 68-69 0.6326270343998424 1101 70-71 0.5625987903138459 1101 72-73 0.6903076168776572 1101 74-75 0.4955504785813183 1101 76-77 0.6675775877896832 1101 78-79 0.5123157378080094 1101 80-81 0.4935702981525374 1101 82-83 0.6036334585316894 1101 84-85 0.3307114022770783 1101 86-87 0.5057925331631559 1101 88-89 0.43680430948063886 1101 90-91 0.4749816986892661 1101 92-93 0.5809755058024386 1101 94-95 0.7080768526164007 1101 96-97 0.6632169240141863 1101 98-99 0.6488412703427002 1101 100-101 0.6712597800106828 1101 102-103 0.8804376379890329 1101 104-105 0.887074591567167 1101 106-107 0.8094440504122282 1101 108-109 0.7177190476796085 1101 110-111 0.7609453571091578 1101 112-113 0.7258009907898391 1101 114-115 0.6323892148562109 1101 116-117 0.5919036986949848 1101 118-119 0.6919102510983883 1101 120-121 0.6292714636913068 1101 122-123 0.8408104805847216 1101 124-125 0.7211456216327861 1102 1 -0.18599497071383908 1102 2 -0.13003922884634278 1102 3 -0.06362792617247948 1102 4 -0.13961151522492088 1102 5 -0.009985775622471493 1102 6 -0.15511613791005274 1102 7 -0.10304407431781115 1102 8 0.07021605271651765 1102 9 -0.23510032801645053 1102 10-11 -0.14014725347330526 1102 12-13 -0.17504674198202608 1102 14-15 -0.11483140114385648 1102 16-17 -0.32360022589627846 1102 18-19 -0.21148701753057964 1102 20-21 -0.38941328582080814 1102 22-23 -0.1135538977679289 1102 24-25 0.02454914251387663 1102 26-27 -0.16531830459424413 1102 28-29 -0.018502561961604158 1102 30-31 -0.045293922472133374 1102 32-33 0.11762270918215734 1102 34-35 0.199172488496977 1102 36-37 0.12199978200566264 1102 38-39 0.03989974864314405 1102 40-41 -0.06528231490348446 1102 42-43 0.23868906029745318 1102 44-45 0.2897514344808094 1102 46-47 0.10920488493439251 1102 48-49 0.08074083761751538 1102 50-51 0.023205328345991205 1102 52-53 0.19412150274192896 1102 54-55 0.2074508132539279 1102 56-57 0.24526957542695627 1102 58-59 0.2608492896249359 1102 60-61 0.35968521217917626 1102 62-63 0.2964817674642859 1102 64-65 0.3325280987436514 1102 66-67 0.28516825614746466 1102 68-69 0.33233997438272667 1102 70-71 0.27858004489175414 1102 72-73 0.3518150256809882 1102 74-75 0.2234597761494861 1102 76-77 0.322869708691492 1102 78-79 0.3734626030225492 1102 80-81 0.27478481566177493 1102 82-83 0.20422772728718286 1102 84-85 0.22690874596448296 1102 86-87 0.15031931081371752 1102 88-89 0.22277155477117105 1102 90-91 0.24660661009415463 1102 92-93 0.2511990143822409 1102 94-95 0.09938161269253953 1102 96-97 0.06977071043300143 1102 98-99 0.1152770563680221 1102 100-101 0.11587331568458481 1102 102-103 0.16433549108351997 1102 104-105 0.13819758647309754 1102 106-107 0.0350587641937139 1102 108-109 0.01210845845988473 1102 110-111 -0.014717595081478407 1102 112-113 -0.06503868939069335 1102 114-115 -0.21865247403330557 1102 116-117 0.044902658072793145 1102 118-119 0.17911699831253358 1102 120-121 0.0480650056705727 1102 122-123 -0.03669240542329533 1102 124-125 0.13047993158649263 1103 1 0.19679038079645395 1103 2 0.27449061714300527 1103 3 0.22293675213237663 1103 4 0.22010308185936367 1103 5 0.30889235239759927 1103 6 0.12464246221743025 1103 7 0.20327056800324783 1103 8 0.096936592267852 1103 9 0.4617001983750946 1103 10-11 0.5949091896825749 1103 12-13 0.5156056956631936 1103 14-15 0.42710514400038235 1103 16-17 0.4331236036022048 1103 18-19 0.3965723248912383 1103 20-21 0.22495864270509003 1103 22-23 0.4419074145097426 1103 24-25 0.3507679651240352 1103 26-27 0.37892634410108883 1103 28-29 0.4221961405654824 1103 30-31 0.48981598232501966 1103 32-33 0.5584807685245821 1103 34-35 0.6471399180201445 1103 36-37 0.5521603725511817 1103 38-39 0.802972148042727 1103 40-41 0.8092216227520161 1103 42-43 1.0474094764757638 1103 44-45 0.7303524667017278 1103 46-47 0.5323332134356136 1103 48-49 0.7070748083781062 1103 50-51 0.741246576058451 1103 52-53 0.8306131853442764 1103 54-55 0.6223337116973724 1103 56-57 0.7127453634721093 1103 58-59 0.7340516419371532 1103 60-61 0.8355657870974404 1103 62-63 0.6909618530029178 1103 64-65 0.7702555677461795 1103 66-67 0.7594758250821378 1103 68-69 0.7564809897426663 1103 70-71 0.6592166496662877 1103 72-73 0.4951179270031396 1103 74-75 0.6657006414055893 1103 76-77 0.5186781103940419 1103 78-79 0.4751990782554145 1103 80-81 0.5572216383953119 1103 82-83 0.6098370871868397 1103 84-85 0.6599377591859614 1103 86-87 0.665082948672783 1103 88-89 0.634744906199213 1103 90-91 0.6222157643721147 1103 92-93 0.5962168006590467 1103 94-95 0.5725993866320422 1103 96-97 0.4783770065693709 1103 98-99 0.383818992913163 1103 100-101 0.5434379323029326 1103 102-103 0.41524022655120874 1103 104-105 0.4496476306817634 1103 106-107 0.6327393514492812 1103 108-109 0.5037242337564933 1103 110-111 0.40732093842457573 1103 112-113 0.6676942285868108 1103 114-115 0.6876611401792658 1103 116-117 0.5253531752113574 1103 118-119 0.43560950340166826 1103 120-121 0.3986397183142536 1103 122-123 0.49469060611207993 1103 124-125 0.40287213441173364 1104 1 0.37504430957702795 1104 2 0.446313298580872 1104 3 0.4414475457500622 1104 4 0.4534602138294872 1104 5 0.3437287434546583 1104 6 0.34031827665224057 1104 7 -0.01381631841307751 1104 8 0.1260958051479939 1104 9 0.34774671000300117 1104 10-11 0.5967406151139585 1104 12-13 0.4485745622330626 1104 14-15 0.5334032259372243 1104 16-17 0.4374743420284446 1104 18-19 0.12441953764302127 1104 20-21 0.31751220969290017 1104 22-23 0.375862587504443 1104 24-25 0.43409742115137817 1104 26-27 0.2994590859447115 1104 28-29 0.2901361680007639 1104 30-31 0.36219458922234793 1104 32-33 0.5614701714461958 1104 34-35 0.4810794480906537 1104 36-37 0.30328385538808433 1104 38-39 0.4572394575399912 1104 40-41 0.3050610060197023 1104 42-43 0.3247456870375487 1104 44-45 0.4092734258135273 1104 46-47 0.4209965055090663 1104 48-49 0.26013801381382606 1104 50-51 0.25468665563681014 1104 52-53 0.3622872991124524 1104 54-55 0.23914624528954675 1104 56-57 0.2750872295526392 1104 58-59 0.3169475081941613 1104 60-61 0.24495956362022042 1104 62-63 0.214554190376667 1104 64-65 0.20586848960375903 1104 66-67 0.18078148782068837 1104 68-69 0.10336684452819966 1104 70-71 0.07477373831754974 1104 72-73 -0.013179641524903474 1104 74-75 0.03717516750520744 1104 76-77 0.0654444371947811 1104 78-79 0.05627474989840309 1104 80-81 -0.002708810757162894 1104 82-83 0.1800842453889473 1104 84-85 0.26730357097350677 1104 86-87 0.23569992164662423 1104 88-89 0.15625753685774058 1104 90-91 0.21348460607374165 1104 92-93 0.23627512068921064 1104 94-95 0.12793763478497766 1104 96-97 0.14115045691712425 1104 98-99 0.12536178633832407 1104 100-101 0.19536802848686108 1104 102-103 0.3271974371370554 1104 104-105 0.3026141708680328 1104 106-107 0.13973695286757604 1104 108-109 0.2536071123754233 1104 110-111 0.17223827158677096 1104 112-113 0.17122391404370418 1104 114-115 0.039721995165344026 1104 116-117 0.14830375159295173 1104 118-119 0.1249354136995251 1104 120-121 0.052692840080379 1104 122-123 0.24722475982988357 1104 124-125 0.1343124065183936 1105 1 -0.21434663456370373 1105 2 -0.02652973502586775 1105 3 -0.08869319304015733 1105 4 -0.304420333090647 1105 5 -0.09203643774506176 1105 6 -0.27968305959988626 1105 7 0.09903253182056915 1105 8 -0.09844407215262052 1105 9 -0.2913418088401194 1105 10-11 0.07680609146818895 1105 12-13 0.12340979055531776 1105 14-15 0.02891223974384971 1105 16-17 -0.35465749103435584 1105 18-19 -0.2878103134434653 1105 20-21 -0.2018705204273985 1105 22-23 -0.22688616172426634 1105 24-25 -0.23599332608867485 1105 26-27 -0.053753224867534755 1105 28-29 -0.08271826305058738 1105 30-31 -0.01694690808746202 1105 32-33 0.011690456539518834 1105 34-35 -0.05484058114968349 1105 36-37 0.009197557081588315 1105 38-39 0.06277782959188372 1105 40-41 -0.06261623406943428 1105 42-43 0.08019150397886676 1105 44-45 0.2262835223773969 1105 46-47 0.05007492385816548 1105 48-49 0.06763399523206104 1105 50-51 -0.012235995015789314 1105 52-53 -0.005431993617154518 1105 54-55 0.09979283411297857 1105 56-57 0.09800473956470057 1105 58-59 0.07984057265260702 1105 60-61 0.13927498986499742 1105 62-63 0.1173347738472259 1105 64-65 0.16076290603494314 1105 66-67 0.1717213053829738 1105 68-69 0.171792778823896 1105 70-71 0.06311336644644072 1105 72-73 0.05173234269160076 1105 74-75 0.14628369082869952 1105 76-77 0.127552650036165 1105 78-79 0.2388024673046587 1105 80-81 0.28404500050901405 1105 82-83 0.2490458595815177 1105 84-85 0.08517987332805887 1105 86-87 0.17370922038936598 1105 88-89 0.08005854533508128 1105 90-91 0.10436900847427921 1105 92-93 0.12359172991013523 1105 94-95 0.23936848570247093 1105 96-97 0.20118590690002947 1105 98-99 0.28204717363528076 1105 100-101 0.15101978546190153 1105 102-103 0.22223915571449382 1105 104-105 0.25469381149481407 1105 106-107 0.06512220614630237 1105 108-109 0.26365808646391997 1105 110-111 0.10559584086554707 1105 112-113 0.12693501633380322 1105 114-115 -0.06390199920667428 1105 116-117 0.1167737036057801 1105 118-119 0.28128202178070794 1105 120-121 0.4330562220559102 1105 122-123 0.24104223564961558 1105 124-125 0.2761453514215759 1106 1 -0.09840899347268817 1106 2 -0.30055836488271837 1106 3 -0.29067444287416677 1106 4 -0.10750109690760823 1106 5 -0.502702254545845 1106 6 -0.18984692436497852 1106 7 -0.06341933124479127 1106 8 -0.2808306017784119 1106 9 -0.12065155839526653 1106 10-11 -0.16152817140595133 1106 12-13 0.06650595819136385 1106 14-15 -0.01671227888680349 1106 16-17 -0.3098748707916137 1106 18-19 -0.09183655305253069 1106 20-21 -0.06935518300408461 1106 22-23 -0.23437560479653285 1106 24-25 -3.6317015033919446 1106 26-27 -0.8476474696820233 1106 28-29 -0.755687216387642 1106 30-31 -0.4935835638652222 1106 32-33 -5.362104460200182 1106 34-35 -1.140645165113778 1106 36-37 -0.46388559698316456 1106 38-39 -0.22049043588496886 1106 40-41 -0.8543725350281868 1106 42-43 -0.42315484292576144 1106 44-45 -0.2590801936611662 1106 46-47 -0.4485626349020926 1106 48-49 -0.1909403547533337 1106 50-51 -0.11935096593444072 1106 52-53 -0.15804931494613328 1106 54-55 -0.359657940061723 1106 56-57 -0.3308083696165056 1106 58-59 -0.13488600955863816 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-1.422347498782493 2216 104-105 -1.5602847446352257 2216 106-107 -1.642310285854382 2216 108-109 -1.5525625027628038 2216 110-111 -1.5082563286732622 2216 112-113 -1.6958769051095466 2216 114-115 -1.6358413859171819 2216 116-117 -1.6855070736631994 2216 118-119 -1.7317320995392187 2216 120-121 -1.553600349310429 2216 122-123 -1.5492408649213587 2216 124-125 -1.911056781037754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45757.0 3 7969.0 4 7519.0 5 6434.0 6 6272.0 7 4927.0 8 4131.0 9 4419.0 10 5399.0 11 6545.0 12 7232.0 13 7588.0 14 7720.0 15 7226.0 16 6559.0 17 5465.0 18 4750.0 19 4052.0 20 3612.0 21 3594.0 22 3932.0 23 6289.0 24 11046.0 25 15532.0 26 17560.0 27 12196.0 28 12770.0 29 15160.0 30 17052.0 31 20427.0 32 25733.0 33 32714.0 34 42954.0 35 59958.0 36 92012.0 37 130786.0 38 46842.0 39 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.93085197958492 20.480193043545867 9.149853106968116 18.43910186990109 2 16.935715644363857 25.573791431621945 37.682036728760586 19.808456195253612 3 16.480158388568476 38.33661458318871 26.902592696465415 18.2806343317774 4 9.149297745788974 20.81160482719937 34.51458656137019 35.52451086564147 5 9.170868922926548 51.560434739145414 28.99265253079495 10.2760438071331 6 24.15397431447414 41.459354390836516 20.602290871225268 13.784380423464073 7 26.754358562108383 41.706826389207066 18.14039311404806 13.398421934636492 8 26.578872467007752 39.28010607501614 18.30627078285861 15.834750675117496 9 28.052793366673978 21.746718464071797 16.51750383898745 33.68298433026678 10-11 21.403941861328843 36.43510840431048 24.761000967034068 17.399948767326606 12-13 22.351469442782186 35.14284841559538 24.962616575886614 17.54306556573582 14-15 21.117629090707133 34.43573580661733 23.67951904252808 20.767116060147455 16-17 19.904558862426057 36.12941340632808 24.442455894573225 19.52357183667264 18-19 19.607668901821423 34.224595218078576 27.05678089119709 19.110954988902904 20-21 22.357827711143646 32.413969772009224 26.403915990316936 18.824286526530194 22-23 21.683295383403152 32.90318008139374 25.81503178552742 19.598492749675682 24-25 20.582099654627815 32.88686023707458 26.707648518717786 19.823391589579824 26-27 20.54346897958815 32.53358065709722 26.844324176476427 20.0786261868382 28-29 20.338774787658323 32.21533025335217 27.181331287726007 20.264563671263495 30-31 20.677058927660283 32.11250299376256 27.476457745427783 19.73398033314937 32-33 20.15319829557524 32.49375557971407 27.157431589060916 20.19561453564977 34-35 20.389324773306935 32.09975189067084 27.59539435776893 19.915528978253295 36-37 20.56433222098731 31.92588123976483 27.142747061955614 20.36703947729224 38-39 20.431865435145557 32.4025421816806 27.192041624580437 19.9735507585934 40-41 20.309492123855165 32.47897068287228 27.481223390112664 19.7303138031599 42-43 19.96179069995078 32.36912238708159 27.373381363386134 20.2957055495815 44-45 20.179716499443593 32.436231812624996 27.376915232375627 20.00713645555578 46-47 20.298594083655217 32.58161840048206 26.781484556579592 20.33830295928313 48-49 19.962707419154864 32.5816504119053 26.843091396673795 20.61255077226604 50-51 20.11017084464314 32.222091094002735 27.353400578969655 20.314337482384467 52-53 20.142784647734697 32.7890976063023 26.823284560611626 20.244833185351382 54-55 19.776269642703426 32.89561008660978 27.01640559700767 20.311714673679123 56-57 20.256065288804102 33.08341872082594 26.31316244727496 20.347353543094997 58-59 20.09843804234641 32.56896910794863 26.768205484206874 20.564387365498092 60-61 20.18236786660141 33.01448460425062 26.664584049233074 20.13856347991489 62-63 19.99505724089911 32.88309333414787 26.889859082771082 20.231990342181934 64-65 19.651980984327622 32.9089424753696 27.061095622618875 20.377980917683907 66-67 19.785907670947587 33.29135716765012 26.53099618188129 20.391738979520998 68-69 20.04398451378577 33.08876666340393 26.47984355508165 20.387405267728653 70-71 19.789087268943238 32.971167670136985 26.669610087046102 20.570134973873675 72-73 20.13149518677079 32.92884667850045 26.586435766801873 20.353222367926893 74-75 19.644235628641088 33.478768542121365 26.671567729072603 20.205428100164944 76-77 19.510740685204624 33.400254355420046 26.87302499680667 20.215979962568657 78-79 20.100436929801056 33.0822641488477 26.390101811446808 20.427197109904434 80-81 19.785991569617302 33.32111538739219 26.463307286894032 20.429585756096476 82-83 19.80268058398883 33.21004340832776 26.413339192381567 20.573936815301835 84-85 19.79160086219763 32.89061822069343 26.58512119013818 20.732659726970752 86-87 19.74135441484369 33.10292786413644 26.574865463754353 20.580852257265512 88-89 19.963485027820106 32.56337187305841 27.006570612389407 20.466572486732083 90-91 19.957931419883998 32.8938809653559 26.531737133852364 20.616450480907737 92-93 19.535606659618203 33.19298883792675 26.532051651402323 20.739352851052722 94-95 19.31689281405284 32.998821247533854 26.962053611716996 20.722232326696314 96-97 19.778021982599135 33.00409648574144 26.711711117574904 20.506170414084522 98-99 19.239446749193338 33.453638448765155 26.085800525371678 21.221114276669834 100-101 19.057232091845698 34.003053100201804 26.464436380903063 20.47527842704944 102-103 19.130592940363027 33.94920787865872 26.508105871645864 20.41209330933239 104-105 18.906685937739717 34.15249277855413 26.556107909678644 20.38471337402751 106-107 18.625464334977902 34.81018062465767 26.114872533930907 20.449482506433526 108-109 18.677989563367795 35.303293363498724 25.47957206614634 20.53914500698714 110-111 18.755610042922687 35.25616505969815 25.599150848630714 20.389074048748455 112-113 18.68543049575773 35.29519570260923 25.69513961105226 20.324234190580786 114-115 18.51037066142036 35.52404502306828 25.510827445356004 20.45475687015535 116-117 18.482288764024663 35.47828598165357 25.57504418610786 20.46438106821391 118-119 18.748051879532742 35.24464922030058 25.585095637529147 20.42220326263753 120-121 18.622145088510933 35.235334953141276 25.505241235682053 20.63727872266574 122-123 18.756599266505194 34.992131209905445 25.55180454577956 20.699464977809804 124-125 18.88079605360894 35.11078745940662 25.09250221797063 20.915914269013804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5196.0 1 5733.0 2 5460.0 3 3699.0 4 2305.5 5 1629.0 6 1303.5 7 1186.0 8 1111.0 9 1072.5 10 977.5 11 867.5 12 837.0 13 839.0 14 909.5 15 960.0 16 1065.0 17 1255.5 18 1433.5 19 1668.5 20 1964.5 21 2407.5 22 2937.5 23 3741.5 24 4867.5 25 5963.0 26 7317.5 27 8752.0 28 9800.5 29 10931.0 30 12889.5 31 14939.5 32 17137.0 33 19928.0 34 22513.5 35 24121.5 36 26238.5 37 28659.0 38 27895.0 39 25810.0 40 24404.5 41 23459.0 42 22816.0 43 22885.5 44 23625.0 45 23446.0 46 22230.5 47 20903.0 48 20617.0 49 20001.5 50 19239.5 51 18297.0 52 16404.5 53 15087.0 54 14118.0 55 13012.5 56 11674.5 57 10295.0 58 9644.0 59 9052.0 60 8219.0 61 7502.0 62 6395.0 63 4597.0 64 3138.0 65 2492.0 66 1968.0 67 1505.0 68 1206.0 69 1040.5 70 942.5 71 833.0 72 720.0 73 758.0 74 655.5 75 442.5 76 304.5 77 189.0 78 139.0 79 101.0 80 54.5 81 30.0 82 23.0 83 14.5 84 9.5 85 5.5 86 4.0 87 3.0 88 3.0 89 4.5 90 4.0 91 2.5 92 2.5 93 2.0 94 2.5 95 3.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1651914946789684E-4 2 2.776794329785979E-4 3 1.3883971648929894E-4 4 4.1651914946789684E-4 5 9.718780154250924E-4 6 6.941985824464946E-4 7 8.330382989357937E-4 8 0.0013883971648929892 9 0.0018049163143608862 10-11 0.0015966567396269375 12-13 0.002221435463828783 14-15 0.0034709929122324736 16-17 0.002846214188030628 18-19 0.002985053904519927 20-21 0.00395693191994502 22-23 0.09253667104011774 24-25 0.002429695038562731 26-27 0.0015966567396269375 28-29 0.0017354964561162368 30-31 0.0010412978736697419 32-33 0.0016660765978715874 34-35 0.0011107177319143915 36-37 7.63618440691144E-4 38-39 0.0010412978736697419 40-41 0.0014578170231376386 42-43 0.0015966567396269375 44-45 0.0013189773066483398 46-47 0.0018049163143608862 48-49 6.247787242018452E-4 50-51 0.0013883971648929892 52-53 0.0013189773066483398 54-55 0.0018049163143608862 56-57 0.0012495574484036904 58-59 6.941985824464946E-4 60-61 0.0010412978736697419 62-63 0.0013189773066483398 64-65 9.718780154250924E-4 66-67 6.941985824464946E-4 68-69 2.0825957473394842E-4 70-71 6.247787242018452E-4 72-73 6.941985824464947E-5 74-75 4.1651914946789684E-4 76-77 4.1651914946789684E-4 78-79 2.776794329785979E-4 80-81 4.1651914946789684E-4 82-83 2.0825957473394842E-4 84-85 3.470992912232473E-4 86-87 4.1651914946789684E-4 88-89 3.470992912232473E-4 90-91 3.470992912232473E-4 92-93 4.859390077125462E-4 94-95 2.776794329785979E-4 96-97 4.859390077125462E-4 98-99 4.1651914946789684E-4 100-101 4.859390077125462E-4 102-103 7.63618440691144E-4 104-105 6.247787242018452E-4 106-107 6.247787242018452E-4 108-109 4.859390077125462E-4 110-111 9.024581571804431E-4 112-113 5.553588659571958E-4 114-115 2.776794329785979E-4 116-117 8.330382989357937E-4 118-119 0.0013189773066483398 120-121 6.941985824464946E-4 122-123 6.247787242018452E-4 124-125 2.776794329785979E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 720255.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.763998042389275 #Duplication Level Percentage of deduplicated Percentage of total 1 69.68372191070868 28.405871035545278 2 9.573166326579276 7.804810667922892 3 5.911856556865347 7.229727273328357 4 4.115121811418067 6.709952698593578 5 2.83945242859018 5.787371662025379 6 1.9410226996640658 4.747430731760348 7 1.32963359623342 3.7940826919767905 8 0.9355671465288565 3.0509965863700828 9 0.6519438913947775 2.3918255561307924 >10 2.80747213928512 19.370999590734343 >50 0.14409276043540398 4.113181397033438 >100 0.06321047820924228 4.414073569966111 >500 0.002378888964862154 0.6533211234331543 >1k 0.0010195238420837804 0.6612174983347001 >5k 3.3984128069459345E-4 0.8651379168447942 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6245 0.8670540294756718 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2366 0.32849476921368126 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1278 0.17743715767332402 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1129 0.15675003991641848 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 967 0.13425800584515207 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 871 0.12092939306217938 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.941985824464947E-5 0.0 22-23 0.0 0.0 0.0 2.776794329785979E-4 0.0 24-25 0.0 0.0 0.0 3.4709929122324736E-4 0.0 26-27 0.0 0.0 0.0 0.0011107177319143913 0.0 28-29 0.0 0.0 0.0 0.0025685347550520304 0.0 30-31 0.0 0.0 0.0 0.008469222705847235 0.0 32-33 0.0 0.0 0.0 0.020409438323926943 0.0 34-35 0.0 0.0 0.0 0.03609832628721772 0.0 36-37 0.0 0.0 0.0 0.05706312347710186 0.0 38-39 0.0 0.0 0.0 0.09489694622043582 0.0 40-41 0.0 0.0 0.0 0.14036695337068122 0.0 42-43 0.0 0.0 0.0 0.19159880875523252 0.0 44-45 0.0 0.0 0.0 0.25546507834031 0.0 46-47 0.0 0.0 0.0 0.3246072571519809 0.0 48-49 0.0 0.0 0.0 0.4129787366974197 0.0 50-51 0.0 0.0 0.0 0.4919091155215861 0.0 52-53 0.0 0.0 0.0 0.585417664577129 0.0 54-55 0.0 0.0 0.0 0.6892697725111245 0.0 56-57 0.0 0.0 0.0 0.8324829400698364 0.0 58-59 0.0 0.0 0.0 0.9989517601405058 0.0 60-61 0.0 0.0 0.0 1.1590339532526674 0.0 62-63 0.0 0.0 0.0 1.3132154584140339 0.0 64-65 0.0 0.0 0.0 1.4792677593352352 0.0 66-67 0.0 0.0 0.0 1.695024678759606 0.0 68-69 0.0 0.0 0.0 1.9835336096243692 0.0 70-71 0.0 0.0 0.0 2.2525355603223858 0.0 72-73 0.0 0.0 0.0 2.5068205010725366 0.0 74-75 0.0 0.0 0.0 2.7815148801466147 0.0 76-77 0.0 0.0 0.0 3.1067469160227974 0.0 78-79 0.0 0.0 0.0 3.5302080513151592 0.0 80-81 0.0 0.0 0.0 3.965956501516824 0.0 82-83 0.0 0.0 0.0 4.374561787144831 0.0 84-85 0.0 0.0 0.0 4.797606403287725 0.0 86-87 0.0 0.0 0.0 5.258415422315707 0.0 88-89 0.0 0.0 0.0 5.81162227266732 0.0 90-91 0.0 0.0 0.0 6.397872975543384 0.0 92-93 0.0 0.0 0.0 6.955522696822653 0.0 94-95 0.0 0.0 0.0 7.54121804083276 0.0 96-97 0.0 0.0 0.0 8.1563474047386 0.0 98-99 0.0 0.0 0.0 8.869011669478171 0.0 100-101 0.0 0.0 0.0 9.646791761251222 0.0 102-103 0.0 0.0 0.0 10.374034196222173 0.0 104-105 0.0 0.0 0.0 11.11918695462024 0.0 106-107 0.0 0.0 0.0 11.919806179755781 0.0 108-109 0.0 0.0 0.0 12.734170536823765 0.0 110-111 0.0 0.0 0.0 13.629686708179742 0.0 112-113 0.0 0.0 0.0 14.518399733427744 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 585 0.0 55.934692 2 GGTATCA 2415 0.0 53.21217 1 GTACATG 11380 0.0 53.0115 1 TACATGG 11510 0.0 50.810394 2 ACATGGG 11715 0.0 46.62027 3 GTCGGGA 450 0.0 46.273243 2 CGGGAGT 445 0.0 45.45622 4 CATGGGA 9770 0.0 44.331375 4 CATGGGG 1780 0.0 43.116558 4 ATGGGAG 2745 0.0 42.697094 5 ATGGGAT 3680 0.0 42.518932 5 CGCCGGT 60 0.0013088174 39.66278 7 TCGGGAG 530 0.0 39.288605 3 TGGGATA 990 0.0 39.06183 6 CATTCCG 245 0.0 38.853336 9 GTATCAA 3380 0.0 38.019943 2 GAGAACA 1510 0.0 37.82411 1 ACCTGGG 840 0.0 36.12146 3 GGAACGC 315 0.0 35.885372 8 CCGGGTC 250 0.0 35.6965 9 >>END_MODULE