FastQCFastQC Report
Thu 26 May 2016
SRR936246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720255
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129411.7967247710880172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107441.4916939139610277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100181.3908962797897968No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29980.4162414700349182No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25170.3494595664035654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.31488847699773No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.23477796058340447No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.21645111800681704No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG12080.16771837751907312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10160.1410611519531277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8880.12328966824249744No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7650.10621238311431369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7480.1038521079339956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7300.10135299303718821No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.081.3433151
AGCGTAC300.00422327159.5449183
ATAGCCG300.00422327159.5449185
ATCACGC401.7649379E-459.5449183
CTACGCG150.00423692659.49944786-87
TATCACG453.1583424E-452.9288182
CACGCAG2050.052.283345
GTATCAA87750.052.046672
ATCCACG1750.047.6359332
GTACATG94000.045.060681
ACATGGG94050.043.7485773
TACATGG96600.043.6416172
CATGGGG14500.043.5293854
TATAACG558.494669E-443.3053972
GCCGGTC558.529598E-443.2693188
TACCTGG4000.043.1700632
TCCACGC2000.041.6814423
ATGGGAT26750.041.18065
GGAACGC2200.040.5649878
ATGGGAG21450.040.5294085
Produced by FastQC (version 0.11.5)