##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936246_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 720255 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486790095174626 31.0 31.0 34.0 30.0 34.0 2 31.22428028961965 31.0 31.0 34.0 28.0 34.0 3 31.613064817321643 31.0 31.0 34.0 30.0 34.0 4 35.50677190717177 37.0 35.0 37.0 33.0 37.0 5 35.17428271931469 37.0 35.0 37.0 33.0 37.0 6 35.427423620800965 37.0 35.0 37.0 33.0 37.0 7 35.2568062700016 37.0 35.0 37.0 32.0 37.0 8 35.36425988018132 37.0 35.0 37.0 33.0 37.0 9 36.959342177423274 39.0 37.0 39.0 33.0 39.0 10-11 36.86174271612137 39.0 37.0 39.0 32.0 39.0 12-13 36.7907935383996 39.0 37.0 39.0 32.0 39.0 14-15 37.74301532096271 40.0 37.0 41.0 32.0 41.0 16-17 37.691092737988626 39.5 36.5 41.0 32.0 41.0 18-19 38.00867539968483 40.0 37.0 41.0 33.0 41.0 20-21 38.09366127274368 40.0 37.0 41.0 33.0 41.0 22-23 37.9164677787728 40.0 37.0 41.0 33.0 41.0 24-25 37.822032474609685 40.0 37.0 41.0 33.0 41.0 26-27 37.5673060235611 40.0 36.0 41.0 32.0 41.0 28-29 37.33304107573012 39.5 36.0 41.0 32.0 41.0 30-31 36.87097347467216 39.0 35.5 41.0 31.5 41.0 32-33 36.296291591172576 38.0 35.0 40.5 30.5 41.0 34-35 35.84395179485043 38.0 35.0 40.0 30.0 41.0 36-37 35.40330855044394 38.0 35.0 40.0 28.5 41.0 38-39 35.22573255305413 38.0 35.0 40.0 27.0 41.0 40-41 35.04260504960049 38.0 35.0 41.0 25.5 41.0 42-43 34.717815218221325 38.0 35.0 41.0 24.0 41.0 44-45 34.165355325544425 38.0 34.0 41.0 19.5 41.0 46-47 33.290663029066096 38.0 33.0 41.0 5.0 41.0 48-49 32.555310272056424 38.0 33.0 41.0 2.0 41.0 50-51 32.282230598885114 38.0 33.0 40.5 2.0 41.0 52-53 32.16428209453596 38.0 32.5 40.0 2.0 41.0 54-55 32.00397498108309 38.0 32.0 40.0 2.0 41.0 56-57 31.823922777349686 38.0 32.0 40.0 2.0 41.0 58-59 31.627138305183582 37.0 31.0 40.0 2.0 41.0 60-61 31.436984817877004 37.0 31.0 40.0 2.0 41.0 62-63 31.241181942506472 36.5 31.0 40.0 2.0 41.0 64-65 31.016285898744194 36.0 31.0 40.0 2.0 41.0 66-67 30.757971829421525 36.0 31.0 39.5 2.0 41.0 68-69 30.474613852038516 35.0 30.0 39.0 2.0 41.0 70-71 30.170940153140208 35.0 30.0 39.0 2.0 41.0 72-73 29.891691137166696 35.0 30.0 38.0 2.0 40.0 74-75 29.6757891302386 35.0 30.0 37.0 2.0 40.0 76-77 29.370781875863408 35.0 29.5 37.0 2.0 39.0 78-79 29.02574504862861 35.0 29.0 36.5 2.0 39.0 80-81 28.73612331743619 35.0 29.0 36.0 2.0 39.0 82-83 28.46067434450299 35.0 29.0 36.0 2.0 37.0 84-85 28.179193480086916 34.5 29.0 35.0 2.0 37.0 86-87 27.947701161394228 34.0 29.0 35.0 2.0 36.5 88-89 27.73260859001326 34.0 28.5 35.0 2.0 36.0 90-91 27.55006837856037 34.0 27.5 35.0 2.0 36.0 92-93 27.3869018611464 34.0 27.0 35.0 2.0 36.0 94-95 27.233742216298396 34.0 27.0 35.0 2.0 35.5 96-97 27.12878702681689 34.0 27.0 35.0 2.0 35.0 98-99 27.002663639960847 34.0 26.0 35.0 2.0 35.0 100-101 26.88609936758509 34.0 25.5 35.0 2.0 35.0 102-103 25.927711019014097 32.5 24.0 34.5 2.0 35.0 104-105 26.552623029343774 34.0 25.0 35.0 2.0 35.0 106-107 26.65592463780189 34.0 25.0 35.0 2.0 35.0 108-109 26.5816974543738 34.0 25.0 35.0 2.0 35.0 110-111 26.50127177180304 34.0 25.0 35.0 2.0 35.0 112-113 26.38985289932038 34.0 24.5 35.0 2.0 35.0 114-115 26.312628166413283 34.0 24.0 35.0 2.0 35.0 116-117 26.18474012676066 34.0 24.0 35.0 2.0 35.0 118-119 26.028598204802467 34.0 21.5 35.0 2.0 35.0 120-121 25.805643140276707 34.0 19.0 35.0 2.0 35.0 122-123 25.564571575344843 34.0 9.5 35.0 2.0 35.0 124-125 23.766768713858287 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1868990204118468 1101 2 0.6130513862697526 1101 3 0.24905141608464376 1101 4 0.21544412067707697 1101 5 0.4422294048530162 1101 6 0.25605019944310214 1101 7 0.3102972538197193 1101 8 0.4497554383798388 1101 9 0.34313587281915403 1101 10-11 0.4729150783686933 1101 12-13 0.4992062709628371 1101 14-15 0.7028775771685858 1101 16-17 0.7653203310131858 1101 18-19 0.5594947063368565 1101 20-21 0.4684093918329637 1101 22-23 0.4279948610669706 1101 24-25 0.5417129288480709 1101 26-27 0.4980739787195958 1101 28-29 0.6533317530015097 1101 30-31 0.4368498516891819 1101 32-33 0.4921276640266967 1101 34-35 0.45331332203158325 1101 36-37 0.6566629930170436 1101 38-39 0.5496178626952286 1101 40-41 0.49359586618327 1101 42-43 0.589669547640213 1101 44-45 0.667918238507788 1101 46-47 0.832437177938445 1101 48-49 1.0277980863948386 1101 50-51 0.9407487995022947 1101 52-53 0.96375834075819 1101 54-55 0.9168758605978695 1101 56-57 0.6461025251754293 1101 58-59 0.5943251989027623 1101 60-61 0.7770329767781519 1101 62-63 0.7877404242510728 1101 64-65 0.7742945144058382 1101 66-67 0.7141180046637317 1101 68-69 0.6019482983612505 1101 70-71 0.46807609840883657 1101 72-73 0.6456874181234404 1101 74-75 0.7356301463241195 1101 76-77 0.7617370036672817 1101 78-79 0.6803917230825114 1101 80-81 0.8058966985276683 1101 82-83 0.6797985563693558 1101 84-85 0.759192511846166 1101 86-87 0.9609310155306616 1101 88-89 0.93856293988582 1101 90-91 0.8829871946163763 1101 92-93 0.9476121565686135 1101 94-95 1.0948699865403668 1101 96-97 1.113350980153271 1101 98-99 0.9035795801240631 1101 100-101 0.9585815056433873 1101 102-103 0.9014791937596023 1101 104-105 0.9686017181718682 1101 106-107 0.9269187327843014 1101 108-109 0.9066264545074709 1101 110-111 0.9768638190244587 1101 112-113 1.0278192023212789 1101 114-115 0.9256091388175065 1101 116-117 0.9324620199968408 1101 118-119 1.0420129774694011 1101 120-121 1.1780396842039629 1101 122-123 1.0528674676275642 1101 124-125 1.0630399562911066 1102 1 0.13108232162575106 1102 2 0.5404590992002554 1102 3 0.3344322150644068 1102 4 0.10703451850765333 1102 5 0.45775340198004244 1102 6 0.30186602116721417 1102 7 0.4565343023402093 1102 8 0.3723796078376935 1102 9 0.40207917905838286 1102 10-11 0.42882019448458664 1102 12-13 0.2958736724652269 1102 14-15 0.4478966947494101 1102 16-17 0.5676969319476157 1102 18-19 0.5459678030530881 1102 20-21 0.5836719296710697 1102 22-23 0.38968028974879587 1102 24-25 0.2596865363936445 1102 26-27 0.5322070977653439 1102 28-29 0.42576616891378194 1102 30-31 0.36324420246182143 1102 32-33 0.34744088182489463 1102 34-35 0.20961335308431472 1102 36-37 0.32426361217539323 1102 38-39 0.24543735859792548 1102 40-41 0.3484029955119894 1102 42-43 -0.10513895654243299 1102 44-45 -0.03041155102103943 1102 46-47 -0.08415185689040072 1102 48-49 -0.29003021331383394 1102 50-51 -0.30966899015927396 1102 52-53 -0.3143094205766488 1102 54-55 -0.40973728158336087 1102 56-57 -0.40061643334901476 1102 58-59 -0.4485927626155508 1102 60-61 -0.31280424525838413 1102 62-63 -0.3283470259401007 1102 64-65 -0.212914603246098 1102 66-67 -0.23129199810967904 1102 68-69 -0.19423790426851184 1102 70-71 -0.22675136927616535 1102 72-73 -0.18053341660252542 1102 74-75 -0.23327445066589902 1102 76-77 -0.18992028791790005 1102 78-79 -0.21108726417164547 1102 80-81 -0.23693237157328895 1102 82-83 -0.3932322616924324 1102 84-85 -0.3481266889370289 1102 86-87 -0.30251707069638556 1102 88-89 -0.3008145357401517 1102 90-91 -0.4230197318028637 1102 92-93 -0.2592843951555217 1102 94-95 -0.3343724452443624 1102 96-97 -0.2762245455505692 1102 98-99 -0.17859875739309317 1102 100-101 -0.18482524014849133 1102 102-103 -0.24370182472269875 1102 104-105 -0.30136507199460993 1102 106-107 -0.36414732420576357 1102 108-109 -0.4010231853045063 1102 110-111 -0.32506713960429323 1102 112-113 -0.39618219149614475 1102 114-115 -0.26493731820763955 1102 116-117 -0.2053801230685579 1102 118-119 -0.19668773816576746 1102 120-121 -0.18534605950904393 1102 122-123 -0.2854200806997369 1102 124-125 -0.15160911224536022 1103 1 0.2697947185578755 1103 2 0.6710494390929185 1103 3 0.3893640514717873 1103 4 0.260808704759782 1103 5 0.39716696889962577 1103 6 0.23250982270115372 1103 7 0.3242064591061933 1103 8 0.38427482302080307 1103 9 0.5347977035170857 1103 10-11 0.4553145501689997 1103 12-13 0.4212000969095371 1103 14-15 0.5132072863230306 1103 16-17 0.5671623155348868 1103 18-19 0.530931716445231 1103 20-21 0.5337521221329808 1103 22-23 0.48686738493108095 1103 24-25 0.3756777357850041 1103 26-27 0.490537860827466 1103 28-29 0.44267855350860685 1103 30-31 0.46530453186777976 1103 32-33 0.4034008267182756 1103 34-35 0.33864610862067934 1103 36-37 0.37721633974721414 1103 38-39 0.5006858524189965 1103 40-41 0.6546508107814617 1103 42-43 0.5072178793508968 1103 44-45 0.5971304472106169 1103 46-47 0.5011657258209254 1103 48-49 0.5386406480354466 1103 50-51 0.4521696790570999 1103 52-53 0.4612236536733505 1103 54-55 0.41593664352922133 1103 56-57 0.4734608392847903 1103 58-59 0.538648411073094 1103 60-61 0.47669054223159435 1103 62-63 0.406724838843072 1103 64-65 0.2750051220433143 1103 66-67 0.3294846350406502 1103 68-69 0.25989303562826294 1103 70-71 0.24817820529253964 1103 72-73 0.4243439300550911 1103 74-75 0.503432106610024 1103 76-77 0.5364332169039301 1103 78-79 0.40059616066334414 1103 80-81 0.480795303050936 1103 82-83 0.5488800900029176 1103 84-85 0.56326064065399 1103 86-87 0.463536843312621 1103 88-89 0.46466877805912077 1103 90-91 0.4768373611175818 1103 92-93 0.39782086259127425 1103 94-95 0.5620713275025366 1103 96-97 0.5263734845935701 1103 98-99 0.5238049602471939 1103 100-101 0.5335809860764975 1103 102-103 0.6195546060958819 1103 104-105 0.4990379465319066 1103 106-107 0.2642171608146917 1103 108-109 0.4432954872018158 1103 110-111 0.49877675240319164 1103 112-113 0.5764958254882053 1103 114-115 0.666441580893796 1103 116-117 0.6512604296425621 1103 118-119 0.6292946194926152 1103 120-121 0.43877233746420785 1103 122-123 0.467566574452114 1103 124-125 0.4752543343855251 1104 1 0.1382080447248768 1104 2 0.45104895777217635 1104 3 0.21270890976293444 1104 4 0.1498570534285335 1104 5 0.34221217293951156 1104 6 0.2459402758646334 1104 7 0.29343803053819784 1104 8 0.24093991202502707 1104 9 0.44637507181569447 1104 10-11 0.27272713028884965 1104 12-13 0.2531581899167392 1104 14-15 0.23542866177727717 1104 16-17 0.3266094384401441 1104 18-19 0.3395541764755592 1104 20-21 0.2842952123725979 1104 22-23 0.35784053525556914 1104 24-25 0.17623366124446704 1104 26-27 0.2575344996042688 1104 28-29 0.40710654391428136 1104 30-31 0.2572528380198591 1104 32-33 0.13312639367400436 1104 34-35 0.15125791541853317 1104 36-37 0.29147426348996675 1104 38-39 0.25515692103125787 1104 40-41 0.27766556326226066 1104 42-43 0.24238357401705457 1104 44-45 0.25306352758315853 1104 46-47 0.16880853769671944 1104 48-49 0.07397560716329821 1104 50-51 0.25651038996783626 1104 52-53 0.37287482448606113 1104 54-55 0.30144969133239385 1104 56-57 0.4415701311817557 1104 58-59 0.44680358888100713 1104 60-61 0.38991964288378256 1104 62-63 0.3918644619689289 1104 64-65 0.3991176869213362 1104 66-67 0.42211761995124775 1104 68-69 0.4479831549155868 1104 70-71 0.3914990777666105 1104 72-73 0.23506133862420953 1104 74-75 0.18550491439458838 1104 76-77 0.183657600612829 1104 78-79 0.043848444931374075 1104 80-81 0.12172320922548252 1104 82-83 0.15740628588441652 1104 84-85 0.13120387689423296 1104 86-87 0.0772119678543639 1104 88-89 0.20515066034410978 1104 90-91 -6.917788424694038E-4 1104 92-93 0.07347164834738606 1104 94-95 0.1346228087671264 1104 96-97 0.17033451365732333 1104 98-99 0.17725482668068437 1104 100-101 0.014095116851343903 1104 102-103 0.0783699955244721 1104 104-105 0.11488944303831872 1104 106-107 0.14521341453490422 1104 108-109 0.0931591129909819 1104 110-111 0.08089488455782501 1104 112-113 0.13515719227867962 1104 114-115 0.2735936060508237 1104 116-117 0.3310866728539352 1104 118-119 0.2808681372252728 1104 120-121 0.32601854104683525 1104 122-123 0.24617186737589947 1104 124-125 0.38808273551823547 1105 1 0.1370282126996294 1105 2 0.6555403369808772 1105 3 0.29538833108716034 1105 4 0.12886453251029195 1105 5 0.4302711881216936 1105 6 0.22975669054297043 1105 7 0.3102001344278733 1105 8 0.1268467752517637 1105 9 0.2317297485016354 1105 10-11 0.08720306399501965 1105 12-13 0.013351632062928331 1105 14-15 0.12016984153070354 1105 16-17 0.14077567606507557 1105 18-19 0.23481048142400596 1105 20-21 0.04363306014204227 1105 22-23 0.18125638870801453 1105 24-25 0.1751937426772514 1105 26-27 0.22143800648007073 1105 28-29 0.34765786582134695 1105 30-31 0.2923526442633033 1105 32-33 0.1584951403088084 1105 34-35 0.2897329373291413 1105 36-37 0.24235088418694062 1105 38-39 0.2805834409197985 1105 40-41 0.37763844520438994 1105 42-43 0.3416250544272117 1105 44-45 0.18816058271927716 1105 46-47 -0.05805130910590606 1105 48-49 -0.15772658153908736 1105 50-51 0.032415799037849524 1105 52-53 0.06703893982819409 1105 54-55 0.3223105521145726 1105 56-57 0.18667650078123188 1105 58-59 0.34185041665775273 1105 60-61 0.2316539409387488 1105 62-63 0.09007113301116476 1105 64-65 0.112175228296028 1105 66-67 0.1329982494046007 1105 68-69 0.1371728752065735 1105 70-71 0.32180842013405453 1105 72-73 0.3706343353268089 1105 74-75 0.30806045728631304 1105 76-77 0.15531451394901552 1105 78-79 0.27053339991146785 1105 80-81 0.21798602643798048 1105 82-83 0.22631091285391491 1105 84-85 0.22382029786081503 1105 86-87 0.25226060350164303 1105 88-89 0.08089493312818874 1105 90-91 0.1464940348761239 1105 92-93 0.2045505147339668 1105 94-95 0.23265773210948737 1105 96-97 0.21965990284075332 1105 98-99 0.2589139802042659 1105 100-101 0.2366821009695954 1105 102-103 0.1532032587700698 1105 104-105 0.20119639587255023 1105 106-107 0.13665393362955314 1105 108-109 0.025021258933296764 1105 110-111 0.11273541465690329 1105 112-113 0.16169280143279252 1105 114-115 0.15259008070329827 1105 116-117 0.1195210176777266 1105 118-119 0.061925977291611645 1105 120-121 0.1605284312776547 1105 122-123 0.015672698603324875 1105 124-125 -0.04504491979160008 1106 1 0.1493353329822078 1106 2 0.49763525637641237 1106 3 0.2721657459013862 1106 4 0.25801227638196167 1106 5 0.3198682985776955 1106 6 0.1534121761616447 1106 7 0.3286369123738524 1106 8 0.18002713272660031 1106 9 0.21152949885352967 1106 10-11 0.25939651471608016 1106 12-13 0.1979876504412701 1106 14-15 0.14993373816576394 1106 16-17 0.20814613791406344 1106 18-19 0.17733298959069543 1106 20-21 0.2009512021227593 1106 22-23 0.15959543207772953 1106 24-25 0.26516266945598943 1106 26-27 0.21696027562197173 1106 28-29 0.022050344508301123 1106 30-31 -0.02872934123585935 1106 32-33 -0.005207619099472538 1106 34-35 0.18199384615446235 1106 36-37 0.3347313054017107 1106 38-39 0.23569193161111457 1106 40-41 0.13892971017664024 1106 42-43 0.3766953011539016 1106 44-45 0.31040051048067596 1106 46-47 0.16967822107760355 1106 48-49 -0.1266533602818143 1106 50-51 -0.1697858847589302 1106 52-53 -0.3617688576912599 1106 54-55 -0.2304820540522705 1106 56-57 -0.21711602571068056 1106 58-59 -0.36551951402506333 1106 60-61 -0.2656663257066718 1106 62-63 -0.3424627952282826 1106 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-1.5322873217169146 2216 32-33 -1.239839653731508 2216 34-35 -1.5080827439221238 2216 36-37 -1.3302626749768862 2216 38-39 -1.0216558488135803 2216 40-41 -1.0535752207898312 2216 42-43 -0.6097541468340069 2216 44-45 -0.9588712434064561 2216 46-47 -0.5499508677822504 2216 48-49 -0.5713624972986437 2216 50-51 -0.4736102655063803 2216 52-53 -0.4154792498631892 2216 54-55 -0.7549729477739326 2216 56-57 -1.087745396340047 2216 58-59 -1.2528552552069563 2216 60-61 -0.9084261300049405 2216 62-63 -0.9405544471660896 2216 64-65 -0.8726584844753908 2216 66-67 -0.6884290255384862 2216 68-69 -0.5014006796010548 2216 70-71 -0.7308807500633705 2216 72-73 -0.5926310448805268 2216 74-75 -0.466200675634564 2216 76-77 -0.5734814659087881 2216 78-79 -0.6510266351454987 2216 80-81 -0.5578775625450056 2216 82-83 -0.5809976351341994 2216 84-85 -0.5558394006285212 2216 86-87 -0.485351477668722 2216 88-89 -0.6108241547683413 2216 90-91 -0.47810081340914223 2216 92-93 -0.6693221911349632 2216 94-95 -0.8676746867694121 2216 96-97 -0.8829180289018765 2216 98-99 -0.8008721515497754 2216 100-101 -1.094134321871369 2216 102-103 -1.0534928294739174 2216 104-105 -0.9504794412681292 2216 106-107 -0.8662994603101559 2216 108-109 -0.9866471104509777 2216 110-111 -0.9326156476833063 2216 112-113 -0.6978018803837394 2216 114-115 -0.7798370957068883 2216 116-117 -0.7585471152275929 2216 118-119 -0.7730132693984579 2216 120-121 -0.8696719016598209 2216 122-123 -0.8305226350861084 2216 124-125 -0.8027784557223967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35.0 3 65.0 4 172.0 5 372.0 6 905.0 7 1824.0 8 2896.0 9 5186.0 10 8984.0 11 11519.0 12 12057.0 13 15617.0 14 22438.0 15 16799.0 16 6313.0 17 4737.0 18 4282.0 19 4246.0 20 4084.0 21 4184.0 22 4586.0 23 4846.0 24 5988.0 25 6940.0 26 8123.0 27 9560.0 28 11412.0 29 13826.0 30 17104.0 31 21128.0 32 25886.0 33 32679.0 34 43441.0 35 61541.0 36 93856.0 37 158461.0 38 73803.0 39 360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.06953607882201 17.736201356211943 12.811909991539306 17.382352573426747 2 18.749765706026665 23.48979311321501 37.86843161184535 19.892009568912975 3 17.5123185880517 31.7070529383425 34.41832245058334 16.362306023022466 4 9.853176999812566 18.020284482579086 37.155035369417774 34.97150314819057 5 8.338890648705423 44.53549179462385 34.20055239714799 12.925065159522738 6 24.674594414478207 38.870538906359556 19.573484390944873 16.881382288217367 7 22.69487889705729 37.81216374756163 23.084185462093288 16.408771893287792 8 28.991954238429447 32.15194618572589 20.846505751435256 18.00959382440941 9 28.312472665930816 10.247065275492707 21.474616628832845 39.96584542974364 10-11 23.905977743993446 27.294777544064253 28.88483939715795 19.914405314784347 12-13 26.432027545799752 27.96710887116369 30.093508549055542 15.507355033981021 14-15 22.610394929573555 26.9529541620676 32.38776544418297 18.048885464175882 16-17 17.404808019382024 28.639231938688383 33.237186829664495 20.718773212265102 18-19 16.686798425557615 23.759779522530213 41.01422412895433 18.539197922957843 20-21 18.853114521940146 22.595539079909198 42.34305905547341 16.208287342677245 22-23 18.111918695462023 23.283212195680697 41.926887005296734 16.677982103560545 24-25 17.043338817502136 23.73103970121693 42.394360330716204 16.831261150564732 26-27 17.15316103324517 23.434130967504565 42.492103491124674 16.920604508125596 28-29 16.964559055167307 22.974240355318848 42.812714117023916 17.248486472489933 30-31 17.237714420587153 23.19921416720467 42.713136319775636 16.849935092432542 32-33 16.952537642918134 23.53930205274521 42.1511825672852 17.35697773705146 34-35 17.34958594439319 23.374686829012283 42.02874404636701 17.246983180227517 36-37 17.8365301178055 23.386925463898205 40.93765402531048 17.838890392985817 38-39 17.832017827019598 23.950128773837044 40.465182470097396 17.752670929045962 40-41 17.78551886867767 24.017690968956114 40.25216086813758 17.944629294228637 42-43 17.710060917405716 24.38869123032333 39.50370537496312 18.397542477307834 44-45 18.087463143943943 24.70999395307279 38.56308069766683 18.639462205316438 46-47 18.752156535491455 25.08947235781445 36.633020663168075 19.52535044352602 48-49 19.066284277400225 25.816343461650344 35.15475032577371 19.96262193517572 50-51 19.35542545488374 26.010414996150438 34.7976948438063 19.836464705159525 52-53 19.163684934721246 26.568654811939147 34.24118219597095 20.02647805736866 54-55 18.97247635087758 26.63870750065605 34.23822264678562 20.150593501680746 56-57 19.457443935572506 26.651282489182133 33.67919963400098 20.212073941244384 58-59 19.51925413233181 25.868501253063236 34.23299339798815 20.379251216616797 60-61 19.679037988673237 26.17491922842493 34.238886705977585 19.907156076924252 62-63 19.727898712454696 25.977009260024168 34.49256608674946 19.802525940771666 64-65 19.49550360086804 25.791573121849154 34.897458218962214 19.815465058320594 66-67 19.680578917736504 26.205899279476785 34.25814016290391 19.85538163988279 68-69 19.956750827843305 26.138258509257266 34.013287145346375 19.891703517553054 70-71 19.66726368988115 25.988837054315226 34.285168832611355 20.05873042319227 72-73 19.985962973350478 26.332753895240185 33.60132345141467 20.07995967999467 74-75 19.42360091496326 26.74920773761797 33.580678863862765 20.246512483556 76-77 19.115644086175273 26.642860217173688 33.946127263614315 20.295368433036725 78-79 19.31414520839509 26.685914492724443 33.49697919277144 20.502961106109023 80-81 18.938024469213552 26.76452130643164 33.41261114165597 20.884843082698833 82-83 19.17733188569588 26.660386474771403 33.100683925392985 21.061597714139737 84-85 18.96540592999548 26.436456053320118 32.98914895935945 21.608989057324955 86-87 18.891152755457288 26.679949045647188 32.97996188836554 21.448936310529987 88-89 19.285301729404317 26.14620241916673 33.1428202508025 21.42567560062645 90-91 19.15817066787367 26.36839613407179 33.02033624856124 21.453096949493293 92-93 19.01997985423127 26.656906173615987 32.5291686711776 21.793945300975146 94-95 18.817685019604358 26.530028545723138 32.82022303428819 21.832063400384314 96-97 19.372146816096677 26.450056091790607 32.408975797225395 21.768821294887317 98-99 18.980039389456483 26.543523127942148 31.99695658482166 22.47948089777971 100-101 19.001375215983884 26.797065743099104 32.22163677775996 21.979922263157054 102-103 19.438998789305906 26.43318690228921 31.929835278959466 22.197979029445413 104-105 19.044858760748877 26.165099732800922 32.27263607958076 22.517405426869438 106-107 18.8480821269252 26.70506531750737 31.972673415239967 22.474179140327468 108-109 19.134774450313536 26.844789632510313 31.142663361339757 22.877772555836398 110-111 18.952820482916323 26.940266491171805 31.00448594860944 23.102427077302433 112-113 18.91364809679789 26.628583647721893 31.14147693427069 23.31629132120953 114-115 18.669806814268416 26.814310074763654 30.92756090516236 23.58832220580557 116-117 18.67479373931204 26.91390408452748 30.83457556087702 23.576726615283462 118-119 18.54279715851972 26.944700550411838 30.67129693913256 23.841205351935884 120-121 18.397699038002948 27.256583226942304 30.35556471941579 23.99015301563896 122-123 18.512146999732025 27.233313755954786 29.855710554706278 24.398828689606912 124-125 18.071676063016287 27.170688665301817 29.897608599516957 24.860026672164935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 454.0 1 378.0 2 1066.0 3 1873.5 4 1371.5 5 1163.5 6 1829.5 7 4431.0 8 6828.0 9 7402.5 10 5801.0 11 3379.5 12 2973.5 13 2911.5 14 2904.0 15 3025.0 16 3168.0 17 3341.0 18 3611.0 19 3805.0 20 4069.5 21 4504.5 22 4925.0 23 5610.5 24 6535.0 25 7444.0 26 8692.0 27 9871.0 28 10424.5 29 11317.5 30 13119.5 31 14540.5 32 16492.0 33 18915.5 34 21108.0 35 22401.0 36 24047.5 37 26704.0 38 26270.0 39 24409.0 40 23048.0 41 21949.0 42 21112.5 43 20958.0 44 21618.0 45 21961.0 46 21605.5 47 20782.0 48 20527.0 49 19638.0 50 18835.0 51 17419.5 52 15096.0 53 14033.5 54 13047.5 55 11933.0 56 10521.5 57 9130.0 58 8622.0 59 8113.5 60 7340.5 61 6612.5 62 5686.0 63 4060.0 64 2729.0 65 2215.5 66 1687.5 67 1250.5 68 1025.5 69 824.0 70 678.0 71 578.0 72 568.0 73 712.5 74 578.5 75 331.0 76 231.5 77 164.0 78 95.5 79 29.0 80 9.5 81 2.5 82 0.0 83 0.5 84 2.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06331091071912032 2 8.330382989357937E-4 3 2.776794329785979E-4 4 0.0 5 0.06705958306433138 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 6.941985824464947E-5 30-31 0.0 32-33 0.0 34-35 2.0825957473394842E-4 36-37 0.0 38-39 0.0 40-41 6.941985824464947E-5 42-43 0.002429695038562731 44-45 0.007566764548666792 46-47 0.008399802847602585 48-49 0.0064560468167524 50-51 0.006039527667284503 52-53 0.0049982297936147614 54-55 0.005275909226593359 56-57 0.004789970218880813 58-59 0.0027767943297859784 60-61 0.0019437560308501849 62-63 0.001180137590159041 64-65 0.0016660765978715874 66-67 0.0020131758890948345 68-69 0.0013883971648929892 70-71 0.0020825957473394837 72-73 0.002360275180318082 74-75 0.0010412978736697419 76-77 2.776794329785979E-4 78-79 6.941985824464947E-5 80-81 4.1651914946789684E-4 82-83 1.3883971648929894E-4 84-85 2.0825957473394842E-4 86-87 3.470992912232473E-4 88-89 9.718780154250924E-4 90-91 0.0019437560308501849 92-93 6.247787242018452E-4 94-95 9.718780154250924E-4 96-97 9.718780154250924E-4 98-99 0.0015966567396269375 100-101 6.247787242018452E-4 102-103 9.718780154250924E-4 104-105 7.63618440691144E-4 106-107 5.553588659571958E-4 108-109 4.859390077125462E-4 110-111 8.330382989357937E-4 112-113 0.01027413902020812 114-115 0.016521926262226573 116-117 0.01534178867206753 118-119 0.009302261004783027 120-121 0.004789970218880813 122-123 0.0049982297936147614 124-125 0.00395693191994502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 720255.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.989341691243403 #Duplication Level Percentage of deduplicated Percentage of total 1 55.78128323988618 16.170626798170893 2 12.989823975812524 7.5313289148787 3 8.488594005068343 7.382362562734995 4 6.141684279412725 7.121735365425338 5 4.3521764862694425 6.308336563052998 6 3.0366729273937003 5.2818689458058605 7 2.083759519053974 4.228477169016567 8 1.4309308997228565 3.318539583089939 9 1.0615176649864535 2.7695428471424823 >10 4.254203666865631 20.853421217719596 >50 0.23679684908568557 4.737256879700498 >100 0.13203218207428297 6.704827137712506 >500 0.005740529655403607 1.079334607694557 >1k 0.003348642298985437 1.8384606057056072 >5k 0.0 0.0 >10k+ 0.0014351324138509017 4.673880802149512 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12941 1.7967247710880172 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10744 1.4916939139610277 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10018 1.3908962797897968 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2998 0.4162414700349182 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2517 0.3494595664035654 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2268 0.31488847699773 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1691 0.23477796058340447 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1559 0.21645111800681704 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1208 0.16771837751907312 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 1016 0.1410611519531277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 888 0.12328966824249744 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 765 0.10621238311431369 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 748 0.1038521079339956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 730 0.10135299303718821 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3883971648929894E-4 0.0 10-11 0.0 0.0 0.0 1.3883971648929894E-4 0.0 12-13 0.0 0.0 0.0 1.3883971648929894E-4 0.0 14-15 0.0 0.0 0.0 1.3883971648929894E-4 0.0 16-17 0.0 0.0 0.0 1.3883971648929894E-4 0.0 18-19 0.0 0.0 0.0 1.3883971648929894E-4 0.0 20-21 0.0 0.0 0.0 2.082595747339484E-4 0.0 22-23 0.0 0.0 0.0 4.165191494678968E-4 0.0 24-25 0.0 0.0 0.0 4.8593900771254627E-4 0.0 26-27 0.0 0.0 0.0 0.0012495574484036904 0.0 28-29 0.0 0.0 0.0 0.0027073744715413292 0.0 30-31 0.0 0.0 0.0 0.008608062422336533 0.0 32-33 0.0 0.0 0.0 0.020548278040416243 0.0 34-35 0.0 0.0 0.0 0.036723105011419566 0.0 36-37 0.0 0.0 0.0 0.05789616177603765 0.0 38-39 0.0 0.0 0.0 0.09621592352708416 0.0 40-41 0.0 0.0 0.0 0.1421718696850421 0.0 42-43 0.0 0.0 0.0 0.19375082436081664 0.0 44-45 0.0 0.0 0.0 0.2577559336623835 0.0 46-47 0.0 0.0 0.0 0.3268981124740543 0.0 48-49 0.0 0.0 0.0 0.4162414700349182 0.0 50-51 0.0 0.0 0.0 0.4966990857404669 0.0 52-53 0.0 0.0 0.0 0.5915960319609028 0.0 54-55 0.0 0.0 0.0 0.6960729186191001 0.0 56-57 0.0 0.0 0.0 0.8418546209328641 0.0 58-59 0.0 0.0 0.0 1.0101283573178943 0.0 60-61 0.0 0.0 0.0 1.1722237263191508 0.0 62-63 0.0 0.0 0.0 1.3288349265190802 0.0 64-65 0.0 0.0 0.0 1.4988441593602266 0.0 66-67 0.0 0.0 0.0 1.719391049003478 0.0 68-69 0.0 0.0 0.0 2.0135229883860575 0.0 70-71 0.0 0.0 0.0 2.2884950468931144 0.0 72-73 0.0 0.0 0.0 2.548333576302837 0.0 74-75 0.0 0.0 0.0 2.8276790858793066 0.0 76-77 0.0 0.0 0.0 3.1581870309820825 0.0 78-79 0.0 0.0 0.0 3.59143636628694 0.0 80-81 0.0 0.0 0.0 4.035168100186739 0.0 82-83 0.0 0.0 0.0 4.450437692206233 0.0 84-85 0.0 0.0 0.0 4.8791053168669425 0.0 86-87 0.0 0.0 0.0 5.350049635198645 0.0 88-89 0.0 0.0 0.0 5.913947143719932 0.0 90-91 0.0 0.0 0.0 6.512554581363545 0.0 92-93 0.0 0.0 0.0 7.078812365065151 0.0 94-95 0.0 0.0 0.0 7.679363558739613 0.0 96-97 0.0 0.0 0.0 8.311709047490126 0.0 98-99 0.0 0.0 0.0 9.040756398775432 0.0 100-101 0.0 0.0 0.0 9.8384599898647 0.0 102-103 0.0 0.0 0.0 10.583890427695746 0.0 104-105 0.0 0.0 0.0 11.346814669804445 0.0 106-107 0.0 0.0 0.0 12.172147364475082 0.0 108-109 0.0 0.0 0.0 13.006643480434013 0.0 110-111 0.0 0.0 0.0 13.931524251827478 0.0 112-113 0.0 0.0 0.0 14.845159006185309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5610 0.0 81.343315 1 AGCGTAC 30 0.004223271 59.544918 3 ATAGCCG 30 0.004223271 59.544918 5 ATCACGC 40 1.7649379E-4 59.544918 3 CTACGCG 15 0.004236926 59.499447 86-87 TATCACG 45 3.1583424E-4 52.928818 2 CACGCAG 205 0.0 52.28334 5 GTATCAA 8775 0.0 52.04667 2 ATCCACG 175 0.0 47.635933 2 GTACATG 9400 0.0 45.06068 1 ACATGGG 9405 0.0 43.748577 3 TACATGG 9660 0.0 43.641617 2 CATGGGG 1450 0.0 43.529385 4 TATAACG 55 8.494669E-4 43.305397 2 GCCGGTC 55 8.529598E-4 43.269318 8 TACCTGG 400 0.0 43.170063 2 TCCACGC 200 0.0 41.681442 3 ATGGGAT 2675 0.0 41.1806 5 GGAACGC 220 0.0 40.564987 8 ATGGGAG 2145 0.0 40.529408 5 >>END_MODULE