FastQCFastQC Report
Thu 26 May 2016
SRR936245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981511
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.057.0347251
GTACATG193950.055.61161
TACATGG200300.053.1667862
ACATGGG198800.051.4431843
CATGGGA139600.048.5833854
TACCTGG18400.044.6200032
CATGGGG52950.043.594744
ATGGGAT59000.041.9478455
GTATCAA54600.041.5145842
ATGGGAG45000.041.5131265
TCAACGC63400.036.5968485
ATGGGGG28650.036.3397375
CAACGCA64850.036.1455276
ATCAACG64900.036.1176834
CCGTTGC2001.8189894E-1235.6969039
ATGGGAC26750.034.4727755
GTCGGGA4150.034.4057852
AACGCAG68500.034.3932277
TGGGATA21850.033.490536
ATGGGAA51950.031.1495445
Produced by FastQC (version 0.11.5)