##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936245_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1981511 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.296489396223386 31.0 30.0 34.0 23.0 34.0 2 29.376146284325447 31.0 30.0 34.0 23.0 34.0 3 29.418891441934967 31.0 30.0 34.0 23.0 34.0 4 32.70823629038648 35.0 33.0 37.0 23.0 37.0 5 33.07539650297172 35.0 35.0 37.0 26.0 37.0 6 32.907910175618504 37.0 35.0 37.0 25.0 37.0 7 32.90066469477081 37.0 35.0 37.0 25.0 37.0 8 32.78708268588971 37.0 35.0 37.0 25.0 37.0 9 33.98218682611401 39.0 35.0 39.0 19.0 39.0 10-11 33.896151472285545 38.0 34.0 39.0 20.0 39.0 12-13 33.90619835065261 38.0 34.0 39.0 20.5 39.0 14-15 34.97945810040923 39.0 34.5 41.0 21.0 41.0 16-17 35.02645430683958 39.0 34.0 41.0 23.0 41.0 18-19 35.08169396990479 39.0 34.0 41.0 23.5 41.0 20-21 35.23235778151118 39.0 34.0 41.0 24.5 41.0 22-23 35.144764525657436 39.0 34.0 41.0 24.5 41.0 24-25 35.166907223830705 39.0 34.0 41.0 25.5 41.0 26-27 35.28121342753081 39.0 34.0 41.0 26.5 41.0 28-29 35.18382638299762 39.0 34.0 41.0 26.0 41.0 30-31 35.11277984326102 39.0 34.0 41.0 26.0 41.0 32-33 34.95533055329998 39.0 34.0 41.0 25.0 41.0 34-35 34.90962124358634 39.0 34.0 41.0 25.0 41.0 36-37 34.796862596271225 39.0 33.5 40.5 25.0 41.0 38-39 34.548089311641476 38.0 33.0 40.0 24.0 41.0 40-41 34.66204830556076 38.5 33.0 40.5 24.5 41.0 42-43 34.57324032013953 39.0 33.0 40.5 24.0 41.0 44-45 34.454696945916524 38.0 33.0 40.0 24.0 41.0 46-47 34.4364235172048 38.0 33.0 40.0 24.0 41.0 48-49 34.35504218750236 38.0 33.0 40.0 24.0 41.0 50-51 34.231539214266284 38.0 33.0 40.0 23.5 41.0 52-53 34.09169997037614 38.0 33.0 40.0 23.0 41.0 54-55 33.9307836292607 38.0 33.0 40.0 23.0 41.0 56-57 33.746571429580754 38.0 32.0 40.0 22.0 41.0 58-59 33.530886025866124 38.0 32.0 40.0 21.5 41.0 60-61 33.29580128497899 37.0 32.0 40.0 20.5 41.0 62-63 33.065961531376814 37.0 31.0 40.0 19.5 41.0 64-65 32.78602415025705 36.5 31.0 40.0 18.0 41.0 66-67 32.48812522362985 36.0 31.0 39.0 17.5 41.0 68-69 32.20415430446765 36.0 31.0 39.0 14.0 41.0 70-71 31.857685624758076 35.0 31.0 39.0 12.5 40.0 72-73 31.519670090148377 35.0 30.5 38.0 9.0 40.0 74-75 31.16403567782364 35.0 30.0 37.0 7.5 39.5 76-77 30.820267462557613 35.0 30.0 37.0 6.5 39.0 78-79 30.463761493123176 34.5 30.0 36.5 2.0 39.0 80-81 30.12936138128933 34.0 30.0 36.0 2.0 38.5 82-83 29.81133766100718 34.0 29.5 35.5 2.0 37.0 84-85 29.806619544378 34.0 30.0 35.0 2.0 37.0 86-87 29.664211049042876 34.0 30.0 35.0 2.0 36.5 88-89 29.476170962462483 34.0 30.0 35.0 2.0 36.0 90-91 29.288625447953606 34.0 30.0 35.0 2.0 36.0 92-93 29.14774558405177 34.0 29.0 35.0 2.0 36.0 94-95 29.012104651450333 34.0 29.0 35.0 2.0 35.0 96-97 28.896846396512558 34.0 29.0 35.0 2.0 35.0 98-99 28.777754451022478 34.0 29.0 35.0 2.0 35.0 100-101 28.66040814307869 34.0 29.0 35.0 2.0 35.0 102-103 27.686594220269278 32.5 26.5 34.5 2.0 35.0 104-105 28.334423326441286 34.0 29.0 35.0 2.0 35.0 106-107 28.450024753836843 34.0 29.0 35.0 2.0 35.0 108-109 28.38800491140347 34.0 29.0 35.0 2.0 35.0 110-111 28.261928901732063 34.0 29.0 35.0 2.0 35.0 112-113 28.207282220487294 34.0 29.0 35.0 2.0 35.0 114-115 28.09317737827345 34.0 29.0 35.0 2.0 35.0 116-117 27.95616274651011 34.0 28.5 35.0 2.0 35.0 118-119 27.74615709930452 34.0 28.0 35.0 2.0 35.0 120-121 27.531386906254873 34.0 27.5 35.0 2.0 35.0 122-123 27.229542253361195 34.0 27.0 35.0 2.0 35.0 124-125 25.278846294570155 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4938410227469774 1101 2 0.7182211797573395 1101 3 0.623066708462158 1101 4 0.6846230512160005 1101 5 0.5047498366588883 1101 6 0.46900867283378744 1101 7 0.39786879126960173 1101 8 0.5733310608338584 1101 9 0.5691232769532988 1101 10-11 0.6740211872333361 1101 12-13 0.4881503447822695 1101 14-15 0.7477360532595014 1101 16-17 0.594286078297074 1101 18-19 0.6400232192281621 1101 20-21 0.508230761223551 1101 22-23 0.5511116612462601 1101 24-25 0.7287960809735807 1101 26-27 0.5807095866539882 1101 28-29 0.7113217046107465 1101 30-31 0.6783294487786478 1101 32-33 0.8097480413211713 1101 34-35 0.7794324972757778 1101 36-37 0.6145946314410367 1101 38-39 0.7865293561060511 1101 40-41 0.778522734368309 1101 42-43 0.8907885736098891 1101 44-45 0.840827770474533 1101 46-47 0.6958509218678017 1101 48-49 0.6532237176128461 1101 50-51 0.6695762228120259 1101 52-53 0.6449233844880169 1101 54-55 0.6786347084486053 1101 56-57 0.6651753416581556 1101 58-59 0.6348382598564726 1101 60-61 0.537716101998555 1101 62-63 0.652916865899094 1101 64-65 0.6088781924410327 1101 66-67 0.645964659221768 1101 68-69 0.5669233260119384 1101 70-71 0.5794842527712021 1101 72-73 0.5982656578730783 1101 74-75 0.6228874863472704 1101 76-77 0.7651358780484472 1101 78-79 0.6537862600355844 1101 80-81 0.5778755681393228 1101 82-83 0.507237010398665 1101 84-85 0.4651553747471162 1101 86-87 0.5423437338092931 1101 88-89 0.4973416111471529 1101 90-91 0.40082145101299105 1101 92-93 0.4381035760804082 1101 94-95 0.5064616054298199 1101 96-97 0.47997851655144075 1101 98-99 0.5465496927785125 1101 100-101 0.5698220735836976 1101 102-103 0.6638701011228108 1101 104-105 0.5978667153758472 1101 106-107 0.5620206745422962 1101 108-109 0.5944214512141919 1101 110-111 0.7278116423798338 1101 112-113 0.7075338642439064 1101 114-115 0.6757091429228979 1101 116-117 0.6627346720718705 1101 118-119 0.6984443245461307 1101 120-121 0.7861781463011219 1101 122-123 0.862629585194675 1101 124-125 0.6172447146170157 1102 1 0.30495476175299885 1102 2 0.3091924942879487 1102 3 0.2580239694193658 1102 4 0.32842480356689663 1102 5 0.3444002857781996 1102 6 0.24351383929375459 1102 7 0.25232914608555745 1102 8 0.472030169281048 1102 9 0.4769648132188067 1102 10-11 0.5920872079392865 1102 12-13 0.43246109623392215 1102 14-15 0.5122262936478208 1102 16-17 0.38691564094478537 1102 18-19 0.46020333495953736 1102 20-21 0.3786211039047629 1102 22-23 0.5573413739904112 1102 24-25 0.6289343101029914 1102 26-27 0.47974507493543683 1102 28-29 0.5196403570188082 1102 30-31 0.5164994087640693 1102 32-33 0.5218282142162209 1102 34-35 0.4723975204588271 1102 36-37 0.5209387551151536 1102 38-39 0.536401907845864 1102 40-41 0.5005254855792742 1102 42-43 0.546640036632347 1102 44-45 0.4895264610585741 1102 46-47 0.42174126287756053 1102 48-49 0.4502130554167607 1102 50-51 0.3063160879592317 1102 52-53 0.3816762869654369 1102 54-55 0.4911959253998148 1102 56-57 0.479511850194271 1102 58-59 0.5980119806102309 1102 60-61 0.5604420071737053 1102 62-63 0.6138558545365598 1102 64-65 0.4986256275970291 1102 66-67 0.45090843991381035 1102 68-69 0.479863419479571 1102 70-71 0.4620820494623956 1102 72-73 0.4193684154355708 1102 74-75 0.40270473807806084 1102 76-77 0.36311559329978493 1102 78-79 0.2867162579295517 1102 80-81 0.3861787596109032 1102 82-83 0.37736660647543374 1102 84-85 0.42174566160181115 1102 86-87 0.4186106948744772 1102 88-89 0.45568358031888323 1102 90-91 0.375598813527656 1102 92-93 0.4419842711372546 1102 94-95 0.4040573462343211 1102 96-97 0.4947533217436373 1102 98-99 0.491848481643963 1102 100-101 0.5113022200698012 1102 102-103 0.5019840674463794 1102 104-105 0.48628910648906754 1102 106-107 0.504636573065067 1102 108-109 0.4901374346467513 1102 110-111 0.47983451788001474 1102 112-113 0.4320567481013491 1102 114-115 0.4625396008006213 1102 116-117 0.42435064079565166 1102 118-119 0.5990933913741507 1102 120-121 0.5167857443903152 1102 122-123 0.5183843128983341 1102 124-125 0.4855952583405454 1103 1 0.10356688509331136 1103 2 0.07162607364818996 1103 3 0.010771067450264127 1103 4 0.022165196591572567 1103 5 0.041045132464120115 1103 6 0.0941096374015089 1103 7 0.10067393049178008 1103 8 0.15764024534178844 1103 9 0.034595070553237406 1103 10-11 0.1558779382019253 1103 12-13 0.06530403212558156 1103 14-15 0.07024662730800912 1103 16-17 -0.006600147622251029 1103 18-19 0.011454477858329426 1103 20-21 -0.19723590454937323 1103 22-23 -0.11220062174276535 1103 24-25 -0.045523392703572085 1103 26-27 -0.15181872531898932 1103 28-29 -0.05359046878353979 1103 30-31 -0.06273246386731302 1103 32-33 0.1390705816399631 1103 34-35 0.0927575998323178 1103 36-37 -0.027523052188797692 1103 38-39 0.08992725369697752 1103 40-41 0.016588596781666354 1103 42-43 0.09719136106983939 1103 44-45 0.19219347335550196 1103 46-47 0.06262838423022288 1103 48-49 -0.12889914411680792 1103 50-51 -0.06407422025903742 1103 52-53 -0.013190689553788104 1103 54-55 0.039954637932602566 1103 56-57 0.040694617930725485 1103 58-59 0.012013815873800127 1103 60-61 0.044304773187541 1103 62-63 0.1420382479322626 1103 64-65 0.011790496544634266 1103 66-67 0.013264749377675855 1103 68-69 -0.034147443028047064 1103 70-71 -0.008699080450412566 1103 72-73 -0.01487142435983202 1103 74-75 0.02081628831259863 1103 76-77 -0.13089826724668896 1103 78-79 -0.011403922019031398 1103 80-81 0.07356488382578164 1103 82-83 -0.003899046268848849 1103 84-85 0.00205624848861774 1103 86-87 -0.03851610726158228 1103 88-89 -0.042713462720371353 1103 90-91 -0.07854030210057417 1103 92-93 -0.009596987310260374 1103 94-95 0.0019772704385978557 1103 96-97 0.028333841447878427 1103 98-99 -0.03480093663711159 1103 100-101 0.01853353947191394 1103 102-103 0.014599433969053166 1103 104-105 0.07314568759844065 1103 106-107 0.07701726436975775 1103 108-109 -0.053657121842260125 1103 110-111 -0.11357344067085506 1103 112-113 0.017975820928850084 1103 114-115 0.014405258845304303 1103 116-117 0.05439927196730565 1103 118-119 0.010917093438656877 1103 120-121 -0.12858932517743682 1103 122-123 0.04943016467264272 1103 124-125 0.09480586311620343 1104 1 0.09511384143300461 1104 2 0.25371170379334984 1104 3 0.22081158996946115 1104 4 0.2210714017748927 1104 5 -0.06408964313516208 1104 6 0.0796690855402673 1104 7 0.019081098357958126 1104 8 -0.07162209892283045 1104 9 0.13501307107414107 1104 10-11 0.22831616536540622 1104 12-13 0.0727446309670654 1104 14-15 0.13577015616706944 1104 16-17 0.06420597975520081 1104 18-19 0.06458640126240311 1104 20-21 0.12935672565183154 1104 22-23 0.28926795383278403 1104 24-25 0.17317602111488384 1104 26-27 0.14572327048411182 1104 28-29 0.17644509196274072 1104 30-31 0.19622582344736372 1104 32-33 0.3048948664688069 1104 34-35 0.3140002828656918 1104 36-37 0.2838657517601746 1104 38-39 0.39834466037113714 1104 40-41 0.3334164970286011 1104 42-43 0.378680743947271 1104 44-45 0.29422441066902394 1104 46-47 0.19827610577091548 1104 48-49 0.21082482429599025 1104 50-51 0.2618394630248986 1104 52-53 0.30886314444605745 1104 54-55 0.3219360255146597 1104 56-57 0.33381830742924734 1104 58-59 0.4144524407209076 1104 60-61 0.3756142814939736 1104 62-63 0.340184114989718 1104 64-65 0.3617749600962128 1104 66-67 0.3238973362746478 1104 68-69 0.2496645770728989 1104 70-71 0.33750455540820923 1104 72-73 0.3033509442520881 1104 74-75 0.32718212139592495 1104 76-77 0.3193150395359403 1104 78-79 0.22243642016197285 1104 80-81 0.23802565985695523 1104 82-83 0.27570082424700715 1104 84-85 0.21712618105136627 1104 86-87 0.21375475897165686 1104 88-89 0.1961643255982004 1104 90-91 0.1315836721641439 1104 92-93 0.11196042213326507 1104 94-95 0.1223315838841792 1104 96-97 0.11033898702068967 1104 98-99 0.14877992212873892 1104 100-101 0.11340926230827009 1104 102-103 0.15944843164147215 1104 104-105 0.15549911542525052 1104 106-107 0.24276237306720105 1104 108-109 0.285711249410447 1104 110-111 0.28448252516049877 1104 112-113 0.26552764549308705 1104 114-115 0.17794713843411714 1104 116-117 0.22353659109337087 1104 118-119 0.23412650745492414 1104 120-121 0.381838640100046 1104 122-123 0.3842303770727895 1104 124-125 0.2755758404338131 1105 1 0.42576933260688676 1105 2 0.44631003933624314 1105 3 0.41142522680103966 1105 4 0.5552255735521499 1105 5 0.4454623786802898 1105 6 0.3972077954313349 1105 7 0.3295670685929508 1105 8 0.3724630617725495 1105 9 0.37360067313758805 1105 10-11 0.4955020709212121 1105 12-13 0.43576654369205414 1105 14-15 0.4024935096749971 1105 16-17 0.3156895592842801 1105 18-19 0.3625215457955946 1105 20-21 0.17870698734543566 1105 22-23 0.21943273717575806 1105 24-25 0.4036799923886889 1105 26-27 0.25951178424317334 1105 28-29 0.35847035285253526 1105 30-31 0.3809287236451482 1105 32-33 0.45931899094414774 1105 34-35 0.5314283981392833 1105 36-37 0.5081907153407315 1105 38-39 0.5515323544682929 1105 40-41 0.3668529600455912 1105 42-43 0.5664083623057721 1105 44-45 0.6031200761491107 1105 46-47 0.4446020854344397 1105 48-49 0.4830442860478925 1105 50-51 0.5089545348784412 1105 52-53 0.4596516635806793 1105 54-55 0.5012962746000298 1105 56-57 0.4649874387918942 1105 58-59 0.5680591752271908 1105 60-61 0.488157236559033 1105 62-63 0.4476409753298327 1105 64-65 0.41771404015062785 1105 66-67 0.4560736251497346 1105 68-69 0.46757664522368714 1105 70-71 0.4153602653371067 1105 72-73 0.46391325256379545 1105 74-75 0.39440516905455425 1105 76-77 0.30551885799158995 1105 78-79 0.17874236722930448 1105 80-81 0.3746814668705625 1105 82-83 0.29095652206567735 1105 84-85 0.2881765793958557 1105 86-87 0.28303685040269144 1105 88-89 0.28035457957938803 1105 90-91 0.22687380604712715 1105 92-93 0.2871926946650163 1105 94-95 0.296335286531928 1105 96-97 0.2828681623858813 1105 98-99 0.1973080268439098 1105 100-101 0.24999209350816542 1105 102-103 0.2596758687014784 1105 104-105 0.2646463877171321 1105 106-107 0.283638464192542 1105 108-109 0.28424108343433474 1105 110-111 0.37023534395906665 1105 112-113 0.3489254095222698 1105 114-115 0.2950748408339834 1105 116-117 0.23254882516651776 1105 118-119 0.3171860166700071 1105 120-121 0.37777182326243874 1105 122-123 0.39752642833578733 1105 124-125 0.4609529174679743 1106 1 -0.4722126989761293 1106 2 -0.5569669278310947 1106 3 -0.31913451383040936 1106 4 -0.4609582379949657 1106 5 -0.28944324388168496 1106 6 -0.47774926965823994 1106 7 -0.40813963305053136 1106 8 -0.47570563306946667 1106 9 -0.49574639775969587 1106 10-11 -0.537952751004525 1106 12-13 -0.5634211212726825 1106 14-15 -0.6781554059742732 1106 16-17 -0.5480984013800594 1106 18-19 -0.6138487572206444 1106 20-21 -0.6477802684078 1106 22-23 -0.7913430588568175 1106 24-25 -4.24007693759971 1106 26-27 -1.6037071099102604 1106 28-29 -1.1960754432634246 1106 30-31 -1.0538162402242222 1106 32-33 -5.846417504890049 1106 34-35 -1.6284654779134229 1106 36-37 -0.8764264157663888 1106 38-39 -0.5483668721859374 1106 40-41 -1.1967027125815832 1106 42-43 -0.7842519889231383 1106 44-45 -0.7396875188921328 1106 46-47 -0.8726289109638969 1106 48-49 -0.7486152613852539 1106 50-51 -0.6258141681411757 1106 52-53 -0.5204650148952794 1106 54-55 -0.7098896371033945 1106 56-57 -0.589145458858205 1106 58-59 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2216 26-27 -0.8868326926751422 2216 28-29 -1.0299874519006167 2216 30-31 -0.8921422048813454 2216 32-33 -0.8272052245561596 2216 34-35 -0.9815546336906564 2216 36-37 -0.9807775781731962 2216 38-39 -0.8864418854584812 2216 40-41 -1.0271761295802548 2216 42-43 -0.7188423470363432 2216 44-45 -0.7343428120702669 2216 46-47 -0.9963830767568851 2216 48-49 -1.0743239480953193 2216 50-51 -1.1896706997922877 2216 52-53 -1.394171778760061 2216 54-55 -1.1669412594762107 2216 56-57 -1.1660921723988764 2216 58-59 -1.2089958762998663 2216 60-61 -1.3511665198942886 2216 62-63 -1.387974242662665 2216 64-65 -1.2501085688882618 2216 66-67 -1.2223729227186269 2216 68-69 -1.1120909416486384 2216 70-71 -1.1605309656227405 2216 72-73 -1.306121119107516 2216 74-75 -1.1451273291088917 2216 76-77 -1.0236870524414563 2216 78-79 -0.8095393253480943 2216 80-81 -0.9572014562484057 2216 82-83 -0.8463296549914787 2216 84-85 -1.0915949570105425 2216 86-87 -0.9272176051429675 2216 88-89 -0.791343746060523 2216 90-91 -0.8382386633081182 2216 92-93 -0.7339720863110131 2216 94-95 -0.7830953961136657 2216 96-97 -1.002157977789949 2216 98-99 -0.8722425261931974 2216 100-101 -0.8986430498201621 2216 102-103 -0.8894970934145547 2216 104-105 -0.9803895880196514 2216 106-107 -1.040179207573427 2216 108-109 -1.0404619715591217 2216 110-111 -0.9577751316037038 2216 112-113 -1.0777989507094148 2216 114-115 -1.1252441712403645 2216 116-117 -1.0358786891451999 2216 118-119 -1.0104460314210897 2216 120-121 -1.107056661529196 2216 122-123 -1.0375694277742582 2216 124-125 -1.1781321987130653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64134.0 3 10735.0 4 7271.0 5 6051.0 6 6855.0 7 6961.0 8 7112.0 9 7602.0 10 9184.0 11 10593.0 12 11974.0 13 12737.0 14 13042.0 15 12712.0 16 11796.0 17 10467.0 18 8901.0 19 8284.0 20 7951.0 21 7930.0 22 9283.0 23 12781.0 24 19817.0 25 29793.0 26 35516.0 27 31497.0 28 35044.0 29 42747.0 30 49644.0 31 60575.0 32 77291.0 33 99787.0 34 136108.0 35 191839.0 36 310738.0 37 443159.0 38 153205.0 39 395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.487280640441845 18.248181813135155 9.459820227735758 21.804717318687242 2 18.431306645591818 21.362910791724893 38.3870575404906 21.818725022192684 3 16.80945339665205 30.969563615626466 29.87454012344121 22.346442864280274 4 10.48767957301164 17.15735405292742 37.34962195421059 35.00534441985035 5 10.273152342044769 44.55368295150883 34.144219389905096 11.028945316541298 6 28.69190007570023 38.88670199343932 19.706081251577086 12.71531667928337 7 28.05360808925799 35.967190379774436 20.94199724248193 15.037204288485645 8 27.8125608760294 34.75578429522086 19.74482787160747 17.686826957142266 9 28.734402402250847 14.89996088772253 18.272852294376662 38.09278441564995 10-11 23.590086897233554 28.770864786067573 28.59758508608597 19.041463230612898 12-13 24.15003545356592 27.885882415283643 27.967791507798523 19.996290623351914 14-15 22.25423023970349 28.76749730500119 25.377198896968277 23.60107355832704 16-17 21.858081382218348 31.00359458528529 26.05053615971112 21.087787872785242 18-19 21.170717527025012 29.999548310922574 27.805802968177623 21.02393119387479 20-21 23.006886652974615 28.93590348238896 27.254476097326112 20.802733767310315 22-23 22.548095015595994 29.249144430272644 27.021985932034298 21.18077462209706 24-25 21.965316022887134 29.204817148949324 27.407382173520823 21.42248465464272 26-27 21.69665774739866 29.250741113451678 27.400127581532573 21.652473557617093 28-29 21.62562831822678 29.036805821910892 27.59505521125623 21.742510648606093 30-31 21.919677575802844 28.84641297529282 27.70378740432069 21.530122044583642 32-33 21.693367355178374 29.11108509229502 27.57990019541028 21.615647357116323 34-35 21.962785085267882 29.15542618044346 27.57671560878036 21.305073125508297 36-37 22.142737104107336 28.69955281420215 27.351485210973692 21.806224870716825 38-39 21.66702287999867 29.011267023485605 27.66112176667009 21.66058832984564 40-41 21.66410882332309 28.76511428033593 27.941944610285102 21.628832286055875 42-43 21.445312145151963 28.72710930505551 27.8895796176801 21.93799893211243 44-45 21.597076130081323 28.86703321021851 27.772878250058604 21.763012409641565 46-47 21.67940002286175 29.030109128273352 27.354463883101253 21.936026965763645 48-49 21.633374219753616 28.83413646845795 27.61756149553963 21.91492781624881 50-51 21.719049964572008 28.708483136881245 27.710468517535343 21.861998381011404 52-53 21.701018883331322 28.631509833796205 27.82014783876521 21.847323444107268 54-55 21.548880342512263 28.95414101555078 27.716427623542344 21.78055101839462 56-57 21.666647320867575 29.28299590054106 27.338844026261167 21.7115127523302 58-59 21.940776549833192 28.63284322371663 27.42983771600793 21.99654251044224 60-61 21.896143332506515 28.924337512594683 27.704498204760508 21.475020950138294 62-63 21.48057979879186 28.94250031731324 27.84950444881996 21.727415435074935 64-65 21.407735705558505 28.91240265517765 27.821025320882754 21.858836318381094 66-67 21.517692134552476 29.289120071541692 27.36409047278277 21.82909732112307 68-69 21.575836762543236 29.009500854400645 27.56610376148243 21.84855862157369 70-71 21.396418617737343 28.809751210889743 27.85279819934166 21.941031972031254 72-73 21.644707375571375 29.041247635953937 27.418846509538824 21.89519847893586 74-75 21.243899201767547 29.35998582893894 27.612145931648858 21.783969037644656 76-77 21.193987594265565 29.03424824489289 27.82004367391823 21.95172048692331 78-79 21.550046340956904 29.029524728256273 27.52027054135776 21.900158389429063 80-81 21.349661330569777 29.17437230145074 27.356687809692083 22.1192785582874 82-83 21.67569723990725 29.06566937983391 27.413065645155875 21.84556773510296 84-85 21.266016538426697 28.896296832738123 27.699530634898245 22.138155993936937 86-87 21.205178988814826 29.09985407567424 27.744975855491766 21.949991080019167 88-89 21.524553691399642 28.803542964449484 27.774955847942813 21.896947496208057 90-91 21.55665746576409 28.85844294889474 27.699250343866805 21.88564924147436 92-93 21.430427881837293 28.855163120961087 27.78955894635643 21.92485005084519 94-95 21.269756437780153 28.784873727356867 27.82608278753635 22.11928704732663 96-97 21.465698682138402 28.98154963486097 27.697399780116683 21.855351902883942 98-99 21.151058841103687 29.079573819105264 27.49986626333708 22.26950107645397 100-101 21.02422148821224 28.99919505628298 27.77502453942836 22.201558916076415 102-103 21.334499121243205 28.68951055832914 27.80199773655464 22.173992583873016 104-105 20.97941236065828 28.85812270423812 27.917171018284336 22.245293916819264 106-107 20.92415132037298 29.081639733848704 27.84308589760962 22.151123048168703 108-109 21.13456196732166 29.431878742122112 27.255139551127026 22.1784197394292 110-111 21.012147679813594 29.457536868098465 27.39439194605876 22.135923506029176 112-113 21.003408012184664 29.202950077895256 27.688219118075285 22.10542279184479 114-115 20.73935795201925 29.516937395502303 27.415704139736352 22.328000512742097 116-117 20.61177437176965 29.670423671781737 27.47877427108827 22.239027685360348 118-119 20.901112954287267 29.223869192922674 27.419614500856177 22.45540335193388 120-121 20.758107303026165 29.436984749688435 27.51407329950207 22.290834647783335 122-123 20.77893960716154 29.329601819630213 27.354104955257412 22.53735361795083 124-125 20.98420545574656 29.16951592903785 27.16276046463626 22.68351815057933 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1245.0 1 1424.0 2 1621.0 3 1490.0 4 1192.5 5 908.5 6 781.0 7 758.0 8 722.5 9 718.5 10 656.5 11 621.0 12 669.5 13 702.5 14 763.0 15 841.5 16 946.5 17 1068.0 18 1289.5 19 1607.0 20 2103.0 21 3030.0 22 4202.5 23 5922.0 24 8118.5 25 10674.5 26 14424.5 27 18005.5 28 20249.0 29 23362.5 30 29300.5 31 35030.5 32 40259.0 33 47304.0 34 55050.0 35 58773.5 36 63197.0 37 69387.0 38 68954.0 39 67099.0 40 65896.0 41 67063.0 42 68526.0 43 69586.5 44 73131.0 45 74809.5 46 73048.0 47 70500.0 48 69133.5 49 66902.5 50 65686.0 51 62400.0 52 58284.5 53 56902.0 54 53836.5 55 48762.5 56 43836.5 57 38915.5 58 34747.5 59 31092.0 60 26314.5 61 21708.0 62 17793.5 63 13746.5 64 10408.0 65 8164.0 66 6127.5 67 4894.5 68 3707.0 69 2438.5 70 1972.5 71 1661.5 72 1361.5 73 1147.0 74 900.0 75 744.0 76 511.5 77 312.0 78 224.5 79 142.5 80 86.5 81 63.5 82 49.0 83 31.0 84 17.0 85 12.5 86 10.5 87 9.5 88 6.0 89 3.0 90 3.5 91 2.5 92 1.5 93 5.5 94 6.5 95 4.5 96 4.0 97 2.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0186615163882512E-4 2 1.0093307581941256E-4 3 5.046653790970628E-5 4 2.523326895485314E-4 5 8.074646065553005E-4 6 5.551319170067691E-4 7 9.588642202844192E-4 8 0.001261663447742657 9 0.001816795364749426 10-11 0.001640162482065454 12-13 0.0023214607438464888 14-15 0.0031793918883114957 16-17 0.002952292467717817 18-19 0.0030027590056275234 20-21 0.0035074243847245867 22-23 0.09265656360222073 24-25 0.002447627088620755 26-27 0.0016653957510203072 28-29 0.0015644626752008945 30-31 0.0011102638340135381 32-33 0.0019681949784785446 34-35 0.0010345640271489788 36-37 4.0373230327765024E-4 38-39 0.0010345640271489788 40-41 0.0012111969098329508 42-43 0.001463529599381482 44-45 0.0013878297925169227 46-47 0.0017915620957945728 48-49 2.7756595850338454E-4 50-51 0.0013625965235620696 52-53 0.0014130630614717758 54-55 0.0017410955578848666 56-57 0.0015139961372911883 58-59 7.822313376004473E-4 60-61 0.0010345640271489788 62-63 0.0015392294062460414 64-65 0.0012111969098329508 66-67 4.541988411873565E-4 68-69 2.523326895485314E-4 70-71 6.812982617810348E-4 72-73 1.7663288268397197E-4 74-75 4.0373230327765024E-4 76-77 4.0373230327765024E-4 78-79 1.261663447742657E-4 80-81 3.2803249641309083E-4 82-83 7.569980686455943E-5 84-85 2.2709942059367824E-4 86-87 2.2709942059367824E-4 88-89 3.2803249641309083E-4 90-91 1.261663447742657E-4 92-93 3.027992274582377E-4 94-95 2.523326895485314E-4 96-97 2.7756595850338454E-4 98-99 2.523326895485314E-4 100-101 3.027992274582377E-4 102-103 9.336309513295661E-4 104-105 3.2803249641309083E-4 106-107 3.2803249641309083E-4 108-109 7.569980686455941E-4 110-111 0.001085030565058685 112-113 2.0186615163882512E-4 114-115 4.0373230327765024E-4 116-117 6.308317238713285E-4 118-119 0.0014130630614717758 120-121 5.29898648051916E-4 122-123 4.794321101422096E-4 124-125 2.0186615163882512E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1981511.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.6928628600384 #Duplication Level Percentage of deduplicated Percentage of total 1 54.10614288279104 16.606724233871482 2 13.480590672904361 8.275158415915325 3 9.49292760679547 8.740953755269377 4 6.68857244519155 8.211657471587838 5 4.61212680295658 7.077968772812682 6 3.108355464452511 5.724259679441513 7 2.034871832418824 4.37192294631302 8 1.3199884081254962 3.2411378549949 9 0.9779587998409062 2.7014719793656203 >10 3.7571162299914937 19.70445598392434 >50 0.2607857667173247 5.566260600326561 >100 0.1547461373662983 8.497037474198086 >500 0.005318354695599295 1.0461867224061754 >1k 4.98595752712434E-4 0.23480410957314474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.523326895485314E-5 0.0 20-21 0.0 0.0 0.0 1.0093307581941256E-4 0.0 22-23 0.0 0.0 0.0 2.0186615163882512E-4 0.0 24-25 0.0 0.0 0.0 3.0279922745823766E-4 0.0 26-27 0.0 0.0 0.0 4.0373230327765024E-4 0.0 28-29 0.0 0.0 0.0 5.29898648051916E-4 0.0 30-31 0.0 0.0 0.0 0.0016906290199751603 0.0 32-33 0.0 0.0 0.0 0.00691391569362976 0.0 34-35 0.0 0.0 0.0 0.013297932739207605 0.0 36-37 0.0 0.0 0.0 0.024905236458440047 0.0 38-39 0.0 0.0 0.0 0.04579838315305845 0.0 40-41 0.0 0.0 0.0 0.06974475539121408 0.0 42-43 0.0 0.0 0.0 0.09946954622003107 0.0 44-45 0.0 0.0 0.0 0.1362848856251618 0.0 46-47 0.0 0.0 0.0 0.18218420185403966 0.0 48-49 0.0 0.0 0.0 0.22866388326887915 0.0 50-51 0.0 0.0 0.0 0.2758248629454997 0.0 52-53 0.0 0.0 0.0 0.3320950527148222 0.0 54-55 0.0 0.0 0.0 0.3945473933780837 0.0 56-57 0.0 0.0 0.0 0.4810722726242751 0.0 58-59 0.0 0.0 0.0 0.583494111312024 0.0 60-61 0.0 0.0 0.0 0.6827870246493711 0.0 62-63 0.0 0.0 0.0 0.78177713875926 0.0 64-65 0.0 0.0 0.0 0.896033380586835 0.0 66-67 0.0 0.0 0.0 1.0413517764978342 0.0 68-69 0.0 0.0 0.0 1.2217444162560793 0.0 70-71 0.0 0.0 0.0 1.4024398552417825 0.0 72-73 0.0 0.0 0.0 1.572991520107635 0.0 74-75 0.0 0.0 0.0 1.7622662705379883 0.0 76-77 0.0 0.0 0.0 1.9835115727341408 0.0 78-79 0.0 0.0 0.0 2.2680671467380193 0.0 80-81 0.0 0.0 0.0 2.5566852770436297 0.0 82-83 0.0 0.0 0.0 2.8424015814194323 0.0 84-85 0.0 0.0 0.0 3.1467148050149607 0.0 86-87 0.0 0.0 0.0 3.4824939149971916 0.0 88-89 0.0 0.0 0.0 3.881028164870142 0.0 90-91 0.0 0.0 0.0 4.3016162918096335 0.0 92-93 0.0 0.0 0.0 4.716955898806517 0.0 94-95 0.0 0.0 0.0 5.15871473839913 0.0 96-97 0.0 0.0 0.0 5.631131999771892 0.0 98-99 0.0 0.0 0.0 6.175085578631661 0.0 100-101 0.0 0.0 0.0 6.761860014907816 0.0 102-103 0.0 0.0 0.0 7.336143983051318 0.0 104-105 0.0 0.0 0.0 7.935156554770577 0.0 106-107 0.0 0.0 0.0 8.56596304537295 0.0 108-109 0.0 0.0 0.0 9.212287996382559 0.0 110-111 0.0 2.523326895485314E-5 0.0 9.943522897425247 0.0 112-113 0.0 5.046653790970628E-5 0.0 10.641424650178575 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3995 0.0 57.034725 1 GTACATG 19395 0.0 55.6116 1 TACATGG 20030 0.0 53.166786 2 ACATGGG 19880 0.0 51.443184 3 CATGGGA 13960 0.0 48.583385 4 TACCTGG 1840 0.0 44.620003 2 CATGGGG 5295 0.0 43.59474 4 ATGGGAT 5900 0.0 41.947845 5 GTATCAA 5460 0.0 41.514584 2 ATGGGAG 4500 0.0 41.513126 5 TCAACGC 6340 0.0 36.596848 5 ATGGGGG 2865 0.0 36.339737 5 CAACGCA 6485 0.0 36.145527 6 ATCAACG 6490 0.0 36.117683 4 CCGTTGC 200 1.8189894E-12 35.696903 9 ATGGGAC 2675 0.0 34.472775 5 GTCGGGA 415 0.0 34.405785 2 AACGCAG 6850 0.0 34.393227 7 TGGGATA 2185 0.0 33.49053 6 ATGGGAA 5195 0.0 31.149544 5 >>END_MODULE