FastQCFastQC Report
Thu 26 May 2016
SRR936245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981511
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113020.5703728114555003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.4260385130337404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84150.42467591651017833No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.1085030565058685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.081.339861
GTATCAA109050.052.7294462
GTACATG174500.048.6488081
ACATGGG177650.047.2449763
TACATGG180300.046.8147052
CATGGGA127700.043.44374
CATGGGG44900.042.821114
TACCTGG16000.042.411792
TCAACGC150100.039.6967665
ATCAACG150250.039.696754
CAACGCA153400.038.7446446
AACGCAG157150.037.8579567
ATGGGAT51150.037.8215645
TATCAAC160450.037.2844773
ATGGGAC23300.036.788185
ATGGGAG41800.036.3132935
ACGCAGA179450.032.9544838
CGCAGAG181000.032.5736669
AGTACAT107800.030.0939712
GAGTACA112900.029.338211
Produced by FastQC (version 0.11.5)