FastQCFastQC Report
Thu 26 May 2016
SRR936244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576465
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.067.762011
GTACATG217550.053.763841
TACATGG217100.052.3691022
ACATGGG219400.049.813473
CATGGGA156800.046.5177464
GTATCAA58800.046.2395022
ATGGGAT60950.045.2916875
TCAACGC67150.040.7554865
CAACGCA68800.040.2104346
ATCAACG68450.040.1552934
ATGGGAG54500.038.862235
AACGCAG72950.038.0044867
CATGGGG60400.035.558594
TATCAAC82400.033.8625683
TACCTGG25700.032.8722532
ACGCAGA88050.031.1497298
CGCAGAG87500.030.8695649
GAGTACA122950.030.4844111
CATGGGT25050.028.7376944
AGTACAT127350.028.3105242
Produced by FastQC (version 0.11.5)