FastQCFastQC Report
Thu 26 May 2016
SRR936244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576465
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116960.45395532250583653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86390.33530438022639547No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84530.3280851864861351No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27960.10852078332133368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73900.081.483271
GTATCAA116350.051.881672
GTACATG188850.045.1319771
TACATGG191850.044.1865962
ACATGGG192200.043.4556963
CATGGGA139900.040.424844
TCAACGC165400.037.755695
ATCAACG165200.037.7293244
CAACGCA167250.037.457896
AACGCAG173250.036.194997
ATGGGAT58250.035.8718345
ATGGGAG48400.035.546345
TATCAAC177050.035.271343
CATGGGG53950.032.110274
GAGTACA119350.031.4398061
ACGCAGA202600.030.7753458
CGCAGAG202800.030.5983077
AGTACAT122400.029.862722
GTCGGGA6700.029.3211882
TACCTGG20750.028.9764842
Produced by FastQC (version 0.11.5)