FastQCFastQC Report
Thu 26 May 2016
SRR936243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212016
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.059.5722471
GTACATG214950.055.798461
TACATGG217450.053.9257622
ACATGGG218000.051.4973073
CATGGGA159300.048.6628724
ATGGGAT68350.042.1284945
GTATCAA53750.041.839112
CATGGGG54950.041.4667664
ATGGGAG56000.040.7955065
TACCTGG20050.038.277582
TCAACGC63800.035.8079645
ATCAACG65100.035.0929034
CAACGCA66300.034.457746
GAGTACA119500.033.5055121
AACGCAG68400.033.399837
ATGGGGG34550.033.2336775
AGTACAT118050.032.8587272
GTCGGGA5750.031.0400582
ATGGGAA59250.031.026965
TATCAAC75300.030.4973033
Produced by FastQC (version 0.11.5)