##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936243_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2212016 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.05395123724241 31.0 30.0 31.0 23.0 34.0 2 29.298768182508624 31.0 30.0 34.0 23.0 34.0 3 29.350056238291224 31.0 30.0 34.0 23.0 34.0 4 32.73855026365089 35.0 33.0 37.0 23.0 37.0 5 33.058805632508985 35.0 35.0 37.0 27.0 37.0 6 32.96018338022871 36.0 35.0 37.0 26.0 37.0 7 32.87321701108853 37.0 35.0 37.0 25.0 37.0 8 32.723818453392745 36.0 33.0 37.0 25.0 37.0 9 34.01153969953201 39.0 35.0 39.0 19.0 39.0 10-11 33.928812675857685 38.0 34.0 39.0 21.5 39.0 12-13 33.92504936673153 38.0 34.0 39.0 20.5 39.0 14-15 35.00467582512965 39.0 34.5 41.0 21.0 41.0 16-17 35.040426922770905 39.0 34.5 41.0 23.0 41.0 18-19 35.10231368127536 39.0 34.0 41.0 23.5 41.0 20-21 35.23237218898959 39.0 34.0 41.0 24.5 41.0 22-23 35.14035183289813 39.0 34.0 41.0 24.5 41.0 24-25 35.14108849122249 39.0 34.0 41.0 25.0 41.0 26-27 35.2412021432033 39.0 34.0 41.0 26.0 41.0 28-29 35.13470698222798 39.0 34.0 41.0 25.5 41.0 30-31 35.05591257025266 39.0 34.0 41.0 25.0 41.0 32-33 34.893740822851186 39.0 34.0 41.0 25.0 41.0 34-35 34.85210821259882 39.0 34.0 41.0 25.0 41.0 36-37 34.743782820739085 39.0 33.5 40.5 24.5 41.0 38-39 34.50071179412807 38.0 33.0 40.0 24.0 41.0 40-41 34.60774063117084 38.5 33.5 40.5 24.0 41.0 42-43 34.529285050379386 39.0 33.0 41.0 24.0 41.0 44-45 34.42319178523121 38.0 33.0 40.0 23.5 41.0 46-47 34.41259217835676 38.0 33.0 40.0 24.0 41.0 48-49 34.326859299390236 38.0 33.0 40.0 23.0 41.0 50-51 34.200693394622824 38.0 33.0 40.0 23.0 41.0 52-53 34.06507909526875 38.0 33.0 40.0 23.0 41.0 54-55 33.89970212692856 38.0 33.0 40.0 22.0 41.0 56-57 33.712250047015935 38.0 32.0 40.0 21.5 41.0 58-59 33.48409279137221 38.0 32.0 40.0 20.0 41.0 60-61 33.23035705890011 37.5 32.0 40.0 18.5 41.0 62-63 32.988197870178155 37.0 31.5 40.0 18.0 41.0 64-65 32.70507288374044 36.5 31.0 40.0 16.0 41.0 66-67 32.41486589608755 36.0 31.0 39.0 13.5 41.0 68-69 32.13326689318703 36.0 31.0 39.0 12.5 41.0 70-71 31.811375686251814 35.0 31.0 39.0 9.0 40.0 72-73 31.48582424358594 35.0 30.5 38.0 7.5 40.0 74-75 31.145664181452574 35.0 30.0 37.5 7.0 39.5 76-77 30.81740977461284 35.0 30.0 37.0 4.0 39.0 78-79 30.469397825332187 35.0 30.0 36.5 2.0 39.0 80-81 30.145993971110517 34.0 30.0 36.0 2.0 38.5 82-83 29.825950626035254 34.0 29.5 36.0 2.0 37.0 84-85 29.817955882778424 34.0 30.0 35.0 2.0 37.0 86-87 29.676107903378636 34.0 30.0 35.0 2.0 36.5 88-89 29.478268918488837 34.0 30.0 35.0 2.0 36.0 90-91 29.285004493638382 34.0 30.0 35.0 2.0 36.0 92-93 29.14245737824681 34.0 29.0 35.0 2.0 36.0 94-95 28.999294308901924 34.0 29.0 35.0 2.0 35.5 96-97 28.876827292388484 34.0 29.0 35.0 2.0 35.0 98-99 28.752613679105394 34.0 29.0 35.0 2.0 35.0 100-101 28.63945333125981 34.0 29.0 35.0 2.0 35.0 102-103 27.67842027363274 32.5 26.5 34.5 2.0 35.0 104-105 28.309330267050512 34.0 29.0 35.0 2.0 35.0 106-107 28.412030021482664 34.0 29.0 35.0 2.0 35.0 108-109 28.345719018307282 34.0 29.0 35.0 2.0 35.0 110-111 28.216691018509813 34.0 29.0 35.0 2.0 35.0 112-113 28.159158206812247 34.0 29.0 35.0 2.0 35.0 114-115 28.024789603691836 34.0 28.5 35.0 2.0 35.0 116-117 27.882669700399997 34.0 28.0 35.0 2.0 35.0 118-119 27.663450671242884 34.0 27.5 35.0 2.0 35.0 120-121 27.435040252873396 34.0 27.0 35.0 2.0 35.0 122-123 27.12508295600032 34.0 27.0 35.0 2.0 35.0 124-125 25.177907619113064 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4902906772339044 1101 2 0.6022087183267644 1101 3 0.48658271236606154 1101 4 0.6044823625396276 1101 5 0.5052397736694232 1101 6 0.5618948998704028 1101 7 0.6661328411174878 1101 8 0.679822623408846 1101 9 0.7226579001102849 1101 10-11 0.8568182239664424 1101 12-13 0.7675299031648777 1101 14-15 0.7376794805529272 1101 16-17 0.7184927276937714 1101 18-19 0.720901709230894 1101 20-21 0.5946120135947268 1101 22-23 0.7329404468042355 1101 24-25 0.7895536943342307 1101 26-27 0.6226959922734352 1101 28-29 0.6345209882626079 1101 30-31 0.7187032857818068 1101 32-33 0.785202775001764 1101 34-35 0.7355356000882338 1101 36-37 0.6254356572704509 1101 38-39 0.8235988111218262 1101 40-41 0.7136856100642177 1101 42-43 0.7830700657513603 1101 44-45 0.7540899144948838 1101 46-47 0.6582603989809499 1101 48-49 0.6437333813272659 1101 50-51 0.6093125269073667 1101 52-53 0.6154450378389313 1101 54-55 0.6269228760376606 1101 56-57 0.7525647778650395 1101 58-59 0.7337868256414168 1101 60-61 0.8382175326063148 1101 62-63 0.7785564491647463 1101 64-65 0.7277776340028623 1101 66-67 0.77806469194676 1101 68-69 0.7088643719323784 1101 70-71 0.7192956808386697 1101 72-73 0.7160946241339019 1101 74-75 0.6974042815757322 1101 76-77 0.6050407938628375 1101 78-79 0.7150497379675294 1101 80-81 0.7070058103503989 1101 82-83 0.6772694965930697 1101 84-85 0.4192082550694529 1101 86-87 0.5363668020472474 1101 88-89 0.5989933028917847 1101 90-91 0.4579469230916722 1101 92-93 0.49809126868368025 1101 94-95 0.6208665283349575 1101 96-97 0.6713227750337047 1101 98-99 0.7170106468069157 1101 100-101 0.6950655334539775 1101 102-103 0.7905691024664776 1101 104-105 0.7651857944075857 1101 106-107 0.7821946820890275 1101 108-109 0.7236432257991616 1101 110-111 0.6911651876695153 1101 112-113 0.6937517249339713 1101 114-115 0.7490132323597471 1101 116-117 0.6775786924672964 1101 118-119 0.5768346820910537 1101 120-121 0.5302423299576517 1101 122-123 0.43794816642203926 1101 124-125 0.25717721224465606 1102 1 0.10901692662007179 1102 2 0.16559078055368914 1102 3 0.1554151076367134 1102 4 0.14013025536124957 1102 5 0.11556324790103645 1102 6 0.08086995621711424 1102 7 0.08454581534279981 1102 8 0.1818803174115473 1102 9 0.1525540206859688 1102 10-11 0.2597939602512156 1102 12-13 0.15781560306036369 1102 14-15 0.28714157400663254 1102 16-17 0.1618833521035441 1102 18-19 0.17450034431821138 1102 20-21 0.1066178308424881 1102 22-23 0.23890992514065346 1102 24-25 0.30325016495208956 1102 26-27 0.2702436823727794 1102 28-29 0.26764064476797955 1102 30-31 0.259944676381032 1102 32-33 0.40403982910720515 1102 34-35 0.3092242640099627 1102 36-37 0.2432794981649522 1102 38-39 0.32212158275078906 1102 40-41 0.20178216126733162 1102 42-43 0.3339080264408736 1102 44-45 0.3042260280193574 1102 46-47 0.272791304683615 1102 48-49 0.32368565974930874 1102 50-51 0.22477444408084324 1102 52-53 0.32021769929273347 1102 54-55 0.3137335888393906 1102 56-57 0.2497621739541458 1102 58-59 0.3203348808926876 1102 60-61 0.37086803185712114 1102 62-63 0.21065166567464644 1102 64-65 0.2727725344674923 1102 66-67 0.21764678187485487 1102 68-69 0.11900715892289782 1102 70-71 0.11452352304267066 1102 72-73 0.13685199957302885 1102 74-75 0.1779800883725926 1102 76-77 0.14887174111727575 1102 78-79 0.14852510280465836 1102 80-81 0.14715300434679435 1102 82-83 0.15682237436790913 1102 84-85 0.15489820331860926 1102 86-87 0.14189784625581225 1102 88-89 0.1544071207439366 1102 90-91 0.12509244242583506 1102 92-93 0.17008046522354903 1102 94-95 0.16008560689298434 1102 96-97 0.16337203276799883 1102 98-99 0.21348252381367416 1102 100-101 0.18272163589797685 1102 102-103 0.11095842995737826 1102 104-105 0.10102885463247091 1102 106-107 0.14871780627845155 1102 108-109 0.17817375375846822 1102 110-111 0.2112115241884851 1102 112-113 0.11577567386307308 1102 114-115 0.09082980018356679 1102 116-117 0.05905088426019489 1102 118-119 0.11209009956042593 1102 120-121 0.13714269385042144 1102 122-123 0.16928424469046277 1102 124-125 0.2081283700280565 1103 1 0.09067239626134338 1103 2 0.1650237462865647 1103 3 0.15362358357367256 1103 4 0.1923988149589988 1103 5 0.10710252199576331 1103 6 0.1351379242739057 1103 7 0.08936602914260305 1103 8 0.12076315004770066 1103 9 0.17467511757192966 1103 10-11 0.3656738774354764 1103 12-13 0.18800320605187437 1103 14-15 0.25034392326931965 1103 16-17 0.2234048799693511 1103 18-19 0.23723326357286822 1103 20-21 0.19900089586973735 1103 22-23 0.3257663074451287 1103 24-25 0.4060566042980156 1103 26-27 0.30621486587724434 1103 28-29 0.32500152700907137 1103 30-31 0.3350302385562145 1103 32-33 0.49314448960494417 1103 34-35 0.34916003956241326 1103 36-37 0.37795351684074063 1103 38-39 0.4040440908844758 1103 40-41 0.3003193649686864 1103 42-43 0.38928893656890295 1103 44-45 0.4385214604780572 1103 46-47 0.33756335014776084 1103 48-49 0.3026948286342517 1103 50-51 0.2813147744987248 1103 52-53 0.1982643465230538 1103 54-55 0.3088402774775716 1103 56-57 0.23644362914490813 1103 58-59 0.2636787774575211 1103 60-61 0.3674028693946241 1103 62-63 0.31721100990923645 1103 64-65 0.18578009774124382 1103 66-67 0.22814621146035563 1103 68-69 0.2660393133996024 1103 70-71 0.31923049900075284 1103 72-73 0.2193597107320251 1103 74-75 0.24013414254084253 1103 76-77 0.20761471656832597 1103 78-79 0.16016831909738727 1103 80-81 0.14511852595916608 1103 82-83 0.22192652783053646 1103 84-85 0.2617250085748779 1103 86-87 0.16069997580328632 1103 88-89 0.2438510722375824 1103 90-91 0.2435151406976921 1103 92-93 0.18738599613156737 1103 94-95 0.2859815801891372 1103 96-97 0.3230826002247973 1103 98-99 0.29505179748587906 1103 100-101 0.3549792097349638 1103 102-103 0.2842419048380158 1103 104-105 0.3266189167442697 1103 106-107 0.2750139147839654 1103 108-109 0.21373043605871445 1103 110-111 0.19900549892123465 1103 112-113 0.30105010661753795 1103 114-115 0.297520973547325 1103 116-117 0.35355715881970795 1103 118-119 0.2199364679214355 1103 120-121 0.2592553401511779 1103 122-123 0.2924202568593053 1103 124-125 0.33817569330519603 1104 1 0.04808734007278659 1104 2 0.1258434323224975 1104 3 0.1592387805121973 1104 4 0.1403050282144065 1104 5 0.1579639357487821 1104 6 0.03455233921273049 1104 7 0.027816399103969047 1104 8 0.030816220253051085 1104 9 0.12059423332351571 1104 10-11 0.13675610382288994 1104 12-13 0.1448601726181451 1104 14-15 0.17011494470862232 1104 16-17 0.11999722728232598 1104 18-19 0.027755633387876344 1104 20-21 0.09482463160639298 1104 22-23 0.08138890527408904 1104 24-25 0.18569367793604385 1104 26-27 0.07880978437597008 1104 28-29 0.06307877567400055 1104 30-31 0.038204305836124774 1104 32-33 0.21557401067586568 1104 34-35 0.22698148351771863 1104 36-37 0.28009944244266904 1104 38-39 0.3438603824895381 1104 40-41 0.22267454451095858 1104 42-43 0.3293506217433304 1104 44-45 0.33429638360027525 1104 46-47 0.2850928663956225 1104 48-49 0.1605356146919732 1104 50-51 0.25694405807589504 1104 52-53 0.2110733752767615 1104 54-55 0.13335576862228748 1104 56-57 0.1970842801571706 1104 58-59 0.22881326577325467 1104 60-61 0.22311544209382106 1104 62-63 0.20785761489626964 1104 64-65 0.27005586656071046 1104 66-67 0.3476991145219941 1104 68-69 0.26475821094965113 1104 70-71 0.27444771097722764 1104 72-73 0.26891016900513875 1104 74-75 0.34959231420491577 1104 76-77 0.30826491389768407 1104 78-79 0.12287722794003741 1104 80-81 0.23130563710058638 1104 82-83 0.19616627343948068 1104 84-85 0.19983127870904127 1104 86-87 0.20898978802024004 1104 88-89 0.1932206660534277 1104 90-91 0.3029214229726449 1104 92-93 0.22943519459018447 1104 94-95 0.29944529670010667 1104 96-97 0.23115289704207953 1104 98-99 0.24768352086221412 1104 100-101 0.15338286261491163 1104 102-103 0.22989325803678184 1104 104-105 0.19707364741006828 1104 106-107 0.2057777532017404 1104 108-109 0.14183993264848382 1104 110-111 0.17918832586180855 1104 112-113 0.18124183125784654 1104 114-115 0.11651090999945168 1104 116-117 0.17090534493630472 1104 118-119 0.19687146814595735 1104 120-121 0.2000719993060791 1104 122-123 0.20579358271967862 1104 124-125 0.2549981667326726 1105 1 0.12475944818593021 1105 2 0.26677965665363956 1105 3 0.28179043406402116 1105 4 0.27493365639343637 1105 5 0.2700220902134376 1105 6 0.3070475079194921 1105 7 0.23331325058332908 1105 8 0.24060233129785757 1105 9 0.202637313796032 1105 10-11 0.44155326420299446 1105 12-13 0.3920066202759287 1105 14-15 0.3154237309640209 1105 16-17 0.3084576908948691 1105 18-19 0.2912672597503345 1105 20-21 0.0819728476124979 1105 22-23 0.12494329189024 1105 24-25 0.27544709808245216 1105 26-27 0.15534881981260185 1105 28-29 0.32787245456760417 1105 30-31 0.3806287195003861 1105 32-33 0.47471124830794054 1105 34-35 0.40418979576158875 1105 36-37 0.4043354518277411 1105 38-39 0.5468919388195488 1105 40-41 0.37788656850008806 1105 42-43 0.6336765879329675 1105 44-45 0.6382653106837495 1105 46-47 0.5089200934062461 1105 48-49 0.5056157578940841 1105 50-51 0.5333454659994317 1105 52-53 0.4889680551139719 1105 54-55 0.5684431768293337 1105 56-57 0.6474920963541635 1105 58-59 0.5824017381557667 1105 60-61 0.4785863204498 1105 62-63 0.5492453363883953 1105 64-65 0.4526144879570069 1105 66-67 0.44084174715908375 1105 68-69 0.47961521100275206 1105 70-71 0.3796369526643275 1105 72-73 0.445596786018406 1105 74-75 0.3921863285488314 1105 76-77 0.33765241909596355 1105 78-79 0.34362925013321544 1105 80-81 0.40266297352958347 1105 82-83 0.4929118061678075 1105 84-85 0.4140541334662622 1105 86-87 0.4946241176205568 1105 88-89 0.5061799149616881 1105 90-91 0.4811348439407084 1105 92-93 0.498161134538762 1105 94-95 0.4666916292447496 1105 96-97 0.48646687305067715 1105 98-99 0.4669225416356788 1105 100-101 0.4524606178160724 1105 102-103 0.5197568061749074 1105 104-105 0.43109209798943837 1105 106-107 0.43232403902885963 1105 108-109 0.4859243447311279 1105 110-111 0.5899134883217059 1105 112-113 0.4728144986010001 1105 114-115 0.48703111455627024 1105 116-117 0.423072221157927 1105 118-119 0.48798645966389387 1105 120-121 0.501741018087106 1105 122-123 0.44045616513502495 1105 124-125 0.5321188897127058 1106 1 -0.2787876865431187 1106 2 -0.3933223657684124 1106 3 -0.1934270764373025 1106 4 -0.19369779796945608 1106 5 -0.22984576204191143 1106 6 -0.447801013435722 1106 7 -0.5236422819227897 1106 8 -0.5318277788667416 1106 9 -0.40449822809738833 1106 10-11 -0.3134571391494063 1106 12-13 -0.3373046625235574 1106 14-15 -0.49494381106126184 1106 16-17 -0.3013138571260683 1106 18-19 -0.425949494628334 1106 20-21 -0.45065289938990105 1106 22-23 -0.5459533953989038 1106 24-25 -4.237477969419555 1106 26-27 -1.4497580865412303 1106 28-29 -1.0581554282429195 1106 30-31 -1.0313010504575075 1106 32-33 -5.705964654884124 1106 34-35 -1.5816005847067558 1106 36-37 -0.7278889312450971 1106 38-39 -0.49249063427438955 1106 40-41 -0.9968657302283077 1106 42-43 -0.5706878589828648 1106 44-45 -0.6509504314375363 1106 46-47 -0.7973374558013191 1106 48-49 -0.6224200143509151 1106 50-51 -0.5552302867356929 1106 52-53 -0.515229362870194 1106 54-55 -0.6875616675914458 1106 56-57 -0.5196625998922642 1106 58-59 -0.4055220345326589 1106 60-61 -0.3915199609191262 1106 62-63 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-0.932746596300511 2216 102-103 -0.9738860304843939 2216 104-105 -1.0024826696510623 2216 106-107 -1.0337953191171714 2216 108-109 -1.0530259218818756 2216 110-111 -0.9766426960037293 2216 112-113 -0.9979060151240091 2216 114-115 -1.070794356336517 2216 116-117 -1.0209133699815105 2216 118-119 -1.0911698391256692 2216 120-121 -1.0841655843541815 2216 122-123 -1.0361667352149624 2216 124-125 -1.1595679316926208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74265.0 3 12644.0 4 8767.0 5 7255.0 6 7843.0 7 8076.0 8 8337.0 9 9179.0 10 10815.0 11 12477.0 12 12994.0 13 13073.0 14 12962.0 15 12723.0 16 12067.0 17 11032.0 18 9960.0 19 9124.0 20 8757.0 21 8887.0 22 10646.0 23 14988.0 24 22599.0 25 33162.0 26 39196.0 27 35269.0 28 39041.0 29 47487.0 30 55671.0 31 67514.0 32 85447.0 33 110009.0 34 149937.0 35 211597.0 36 344303.0 37 496547.0 38 176816.0 39 550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.77106411911711 18.298306335239296 9.23974958499246 21.690879960651138 2 18.52465671555424 21.209178603752054 38.20626813392682 22.059896546766883 3 16.956703748978306 30.588657586563567 29.87853614078741 22.576102523670716 4 10.1317805977369 16.64861370427801 37.803174488361265 35.41643120962383 5 10.307847544579484 44.49978390635822 34.31729779873815 10.875070750324141 6 29.20717284928445 38.27323409318592 19.92343608160549 12.596156975924139 7 27.955148363203687 36.143108240310596 21.01519707920962 14.8865463172761 8 27.43656523946461 35.28815816657979 19.506762441359548 17.768514152596047 9 28.36923165948459 14.558261618055793 18.335045217243493 38.73746150521612 10-11 23.447549259277093 28.753168237986127 28.869420787438667 18.929861715298117 12-13 24.229985128751917 27.66974391570654 28.199177526625952 19.90109342891559 14-15 22.248305019997982 28.76359702401431 25.43756185089058 23.550536105097123 16-17 21.884703567149106 30.919067178154926 25.990940681331832 21.20528857336414 18-19 21.288891953014893 29.902112476279708 27.726253996706568 21.08274157399883 20-21 22.853881381754725 28.94603000944859 27.325777474378043 20.87431113441864 22-23 22.468885033164185 29.28364591777472 26.902865375604723 21.344603673456366 24-25 21.791188767908984 29.23787632231434 27.47965796309059 21.491276946686085 26-27 21.71053925363671 29.15305996742756 27.432371493122147 21.70402928581358 28-29 21.726558666145568 28.955529068662212 27.529599404700416 21.788312860491803 30-31 21.99670479129855 28.88703184607411 27.62652071123886 21.489742651388482 32-33 21.637689424634647 29.149507378974043 27.578346148690695 21.634457047700618 34-35 21.953074688591276 28.976771973113074 27.6349121351245 21.435241203171152 36-37 22.008308749684392 28.804087512440635 27.339172529182225 21.848431208692745 38-39 21.72240350596428 29.09174584833106 27.57135843457646 21.6144922111282 40-41 21.732125165489414 28.821887165887695 27.952515359971823 21.493472308651064 42-43 21.510788667434902 28.782430536750276 27.833762884850206 21.873017910964617 44-45 21.572264918625677 28.99231464737794 27.79593128390597 21.639489150090416 46-47 21.71124786052765 28.960001699823778 27.429346553381244 21.899403886267322 48-49 21.550131735270874 28.91699954611458 27.52785699173422 22.005011726880326 50-51 21.53920677541759 28.813494958061643 27.82382341020883 21.823474856311933 52-53 21.719295921764868 28.715571009654845 27.83905594872691 21.726077119853382 54-55 21.610874334170813 28.979169280254613 27.734705848446932 21.675250537127642 56-57 21.564989543409094 29.189960045244085 27.581462403741046 21.66358800760578 58-59 21.804167800629838 28.698814560177503 27.514346706112008 21.98267093308065 60-61 21.936023594904707 29.02737925762307 27.530387617014984 21.506209530457237 62-63 21.49527576853526 28.86381103074141 27.85736889692586 21.78354430379747 64-65 21.30134179384598 28.90581222481088 27.908638398301267 21.884207583041874 66-67 21.469771836474518 29.277467100058317 27.31185663717614 21.94090442629102 68-69 21.54095769737443 28.925178592902217 27.595869646394643 21.937994063328713 70-71 21.39325445477593 28.644268013497236 27.94867830496092 22.013799226765908 72-73 21.571409939405886 28.996093378272782 27.439931989565164 21.99256469275617 74-75 21.21569467782936 29.208466865671102 27.607435044400773 21.968403412098763 76-77 21.198719174542983 29.03177244597789 27.793510068439986 21.97599831103914 78-79 21.55759100764372 28.963495677238644 27.476116766093494 22.002796549024144 80-81 21.32648254436693 29.022001728746012 27.328902065453654 22.32261366143341 82-83 21.48621120842816 29.08722548772295 27.418542558953064 22.00802074489583 84-85 21.204189310089326 28.89309772871835 27.72687387178454 22.175839089407784 86-87 20.996138582462162 29.074238538090786 27.705823410050083 22.223799469396972 88-89 21.327186585955758 28.867997884277198 27.735227236766562 22.069588293000482 90-91 21.478391952302236 28.867119361721223 27.63023609958628 22.02425258639026 92-93 21.331286026315865 28.978850245579647 27.605304041140833 22.084559686963654 94-95 21.087929323992505 28.79194234925302 27.823388642770297 22.29673968398418 96-97 21.38254831184043 28.866422137879606 27.65894540688003 22.092084143399926 98-99 21.00095908311383 28.838700212951263 27.608551232963162 22.551789470971745 100-101 20.98112598521842 28.97006093328261 27.640251997109416 22.408561084389554 102-103 21.1412366342918 28.721331426163786 27.599113744821434 22.538318194722976 104-105 20.893952261033355 28.692769489809827 27.816755677023846 22.596522572132972 106-107 20.71409777903154 28.95893685737122 27.83195178317619 22.49501358042105 108-109 20.86655260732733 29.13176340123774 27.304372749356748 22.69731124207818 110-111 20.7586338623035 29.200848552386354 27.340701468465788 22.699816116844353 112-113 20.69721188032335 29.095589736124673 27.61833635057338 22.588862032978597 114-115 20.638749661506804 29.262245316361735 27.433093626653417 22.665911395478048 116-117 20.460623349205076 29.359483092808443 27.457360468625147 22.722533089361335 118-119 20.575064461026255 29.016944202644172 27.34476629812312 23.063225038206454 120-121 20.475953457546602 29.126216884071532 27.417561105279294 22.980268553102572 122-123 20.442915212385515 29.00070004410029 27.33345584670187 23.222928896812327 124-125 20.462606303179847 28.99687863145315 27.084184274893815 23.456330790473192 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1380.0 1 1771.0 2 2112.0 3 1829.5 4 1358.5 5 1039.0 6 879.5 7 801.0 8 791.0 9 786.0 10 785.5 11 784.5 12 787.0 13 773.0 14 822.5 15 990.0 16 1140.5 17 1302.0 18 1646.5 19 2050.0 20 2579.5 21 3381.0 22 4478.0 23 6050.0 24 8102.5 25 11040.5 26 15083.5 27 19168.5 28 22389.5 29 26156.0 30 32377.5 31 39011.5 32 44812.0 33 51790.5 34 60117.0 35 65526.5 36 71705.0 37 78595.5 38 78415.5 39 76915.0 40 76251.5 41 75623.5 42 75317.5 43 76305.5 44 79990.0 45 81733.0 46 80456.0 47 78169.5 48 76966.5 49 74659.0 50 72610.0 51 69872.5 52 65695.5 53 63422.0 54 59356.5 55 54440.5 56 49856.5 57 44966.5 58 41027.0 59 36451.0 60 30518.0 61 25353.0 62 20744.5 63 15418.5 64 10871.0 65 8319.5 66 6564.5 67 5064.0 68 3817.5 69 2679.0 70 2036.5 71 1550.0 72 1321.0 73 1183.0 74 804.0 75 545.0 76 394.5 77 261.0 78 184.5 79 118.5 80 62.0 81 37.0 82 28.0 83 26.0 84 21.0 85 15.5 86 13.5 87 11.5 88 9.0 89 6.5 90 5.5 91 5.5 92 4.5 93 4.5 94 3.5 95 1.0 96 1.5 97 2.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.6166103680986033E-4 2 9.041525920246508E-5 3 0.0 4 2.7124577760739523E-4 5 5.424915552147905E-4 6 3.6166103680986033E-4 7 5.424915552147905E-4 8 8.589449624234184E-4 9 0.0012658136288345111 10-11 9.719640364264996E-4 12-13 0.00133362507323636 14-15 0.0019439280728529992 16-17 0.0017857013692486854 18-19 0.0017404937396474527 20-21 0.002079550961656697 22-23 0.09161326138689774 24-25 0.0014692479620400576 26-27 0.0010623792956289647 28-29 9.267564068252671E-4 30-31 7.459258884203369E-4 32-33 9.719640364264996E-4 34-35 5.198877404141742E-4 36-37 4.7468011081294167E-4 38-39 6.329068144172556E-4 40-41 7.459258884203369E-4 42-43 8.589449624234184E-4 44-45 7.233220736197207E-4 46-47 9.94567851227116E-4 48-49 1.8083051840493016E-4 50-51 8.815487772240346E-4 52-53 7.911335180215695E-4 54-55 8.36341147622802E-4 56-57 6.329068144172556E-4 58-59 4.5207629601232537E-4 60-61 5.876991848160231E-4 62-63 7.233220736197207E-4 64-65 6.555106292178718E-4 66-67 2.7124577760739523E-4 68-69 9.041525920246508E-5 70-71 3.6166103680986033E-4 72-73 1.1301907400308134E-4 74-75 2.2603814800616268E-4 76-77 2.2603814800616268E-4 78-79 1.8083051840493016E-4 80-81 3.6166103680986033E-4 82-83 9.041525920246508E-5 84-85 2.2603814800616268E-4 86-87 2.0343433320554644E-4 88-89 2.7124577760739523E-4 90-91 2.0343433320554644E-4 92-93 2.0343433320554644E-4 94-95 2.9384959240801153E-4 96-97 2.9384959240801153E-4 98-99 3.390572220092441E-4 100-101 2.9384959240801153E-4 102-103 5.424915552147905E-4 104-105 3.390572220092441E-4 106-107 3.6166103680986033E-4 108-109 4.294724812117092E-4 110-111 6.103029996166393E-4 112-113 2.48641962806779E-4 114-115 3.164534072086278E-4 116-117 6.103029996166393E-4 118-119 9.267564068252671E-4 120-121 6.329068144172556E-4 122-123 5.650953700154067E-4 124-125 2.48641962806779E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2212016.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.53808273728697 #Duplication Level Percentage of deduplicated Percentage of total 1 53.28082405407238 16.803750373281648 2 13.605527618017362 8.581845114029491 3 9.686057391429673 9.164390382270572 4 6.976961439879843 8.801599485831654 5 4.700561456680035 7.412334806623855 6 3.141486610298832 5.944587878021026 7 2.10262508949611 4.641893482661661 8 1.4153265175236744 3.570934784795031 9 0.9761790628400281 2.770813444523046 >10 3.785224714384795 19.847429485247826 >50 0.20523921638709977 4.457419137193524 >100 0.11878041675459225 6.7530271474003145 >500 0.004627921987223365 0.9950535747007259 >1k 5.784902484029193E-4 0.2549209034196162 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.520762960123254E-5 0.0 5 0.0 0.0 0.0 4.520762960123254E-5 0.0 6 0.0 0.0 0.0 4.520762960123254E-5 0.0 7 0.0 0.0 0.0 4.520762960123254E-5 0.0 8 0.0 0.0 0.0 4.520762960123254E-5 0.0 9 0.0 0.0 0.0 4.520762960123254E-5 0.0 10-11 0.0 0.0 0.0 4.520762960123254E-5 0.0 12-13 0.0 0.0 0.0 9.041525920246508E-5 0.0 14-15 0.0 0.0 0.0 9.041525920246508E-5 0.0 16-17 0.0 0.0 0.0 9.041525920246508E-5 0.0 18-19 0.0 0.0 0.0 9.041525920246508E-5 0.0 20-21 0.0 0.0 0.0 9.041525920246508E-5 0.0 22-23 0.0 0.0 0.0 9.041525920246508E-5 0.0 24-25 0.0 0.0 0.0 2.260381480061627E-4 0.0 26-27 0.0 0.0 0.0 3.6166103680986033E-4 0.0 28-29 0.0 0.0 0.0 7.007182588191044E-4 0.0 30-31 0.0 0.0 0.0 0.0022829852948622435 0.0 32-33 0.0 0.0 0.0 0.007911335180215695 0.0 34-35 0.0 0.0 0.0 0.015958293249235086 0.0 36-37 0.0 0.0 0.0 0.027599257871552464 0.0 38-39 0.0 0.0 0.0 0.04846257893252129 0.0 40-41 0.0 0.0 0.0 0.07339458665760104 0.0 42-43 0.0 0.0 0.0 0.10194320475077939 0.0 44-45 0.0 0.0 0.0 0.1410252005410449 0.0 46-47 0.0 0.0 0.0 0.18876445739994646 0.0 48-49 0.0 0.0 0.0 0.23668454477725298 0.0 50-51 0.0 0.0 0.0 0.28541836948738164 0.0 52-53 0.0 0.0 0.0 0.339238052527649 0.0 54-55 0.0 0.0 0.0 0.40492473833823983 0.0 56-57 0.0 0.0 0.0 0.49255972832022915 0.0 58-59 0.0 0.0 0.0 0.5934405537753795 0.0 60-61 0.0 0.0 0.0 0.6941857563417262 0.0 62-63 0.0 0.0 0.0 0.7932356727980268 0.0 64-65 0.0 0.0 0.0 0.9088315816883783 0.0 66-67 0.0 0.0 0.0 1.0550782634483657 0.0 68-69 0.0 0.0 0.0 1.2398644494434037 0.0 70-71 0.0 0.0 0.0 1.421689535699561 0.0 72-73 0.0 0.0 0.0 1.5951286066646895 0.0 74-75 0.0 0.0 0.0 1.7854527272858784 0.0 76-77 0.0 0.0 0.0 2.016102053511367 0.0 78-79 0.0 0.0 0.0 2.2990566071854817 0.0 80-81 0.0 0.0 0.0 2.587775133633753 0.0 82-83 0.0 0.0 0.0 2.8712269712334813 0.0 84-85 0.0 0.0 0.0 3.1686027587503887 0.0 86-87 0.0 0.0 0.0 3.517944716493913 0.0 88-89 0.0 0.0 0.0 3.9207673000556955 0.0 90-91 0.0 0.0 0.0 4.341153047717556 0.0 92-93 0.0 0.0 0.0 4.758374261307333 0.0 94-95 0.0 0.0 0.0 5.183710244410529 0.0 96-97 0.0 0.0 0.0 5.670212150364193 0.0 98-99 0.0 0.0 0.0 6.201356590549073 0.0 100-101 0.0 0.0 0.0 6.7815513088512915 0.0 102-103 0.0 0.0 0.0 7.349472155716776 2.260381480061627E-5 104-105 0.0 0.0 0.0 7.948744493710715 4.520762960123254E-5 106-107 0.0 0.0 0.0 8.59225249726946 4.520762960123254E-5 108-109 0.0 0.0 0.0 9.263382362514557 4.520762960123254E-5 110-111 0.0 0.0 0.0 9.99264923942684 4.520762960123254E-5 112-113 0.0 0.0 0.0 10.687038430101772 4.520762960123254E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3775 0.0 59.572247 1 GTACATG 21495 0.0 55.79846 1 TACATGG 21745 0.0 53.925762 2 ACATGGG 21800 0.0 51.497307 3 CATGGGA 15930 0.0 48.662872 4 ATGGGAT 6835 0.0 42.128494 5 GTATCAA 5375 0.0 41.83911 2 CATGGGG 5495 0.0 41.466766 4 ATGGGAG 5600 0.0 40.795506 5 TACCTGG 2005 0.0 38.27758 2 TCAACGC 6380 0.0 35.807964 5 ATCAACG 6510 0.0 35.092903 4 CAACGCA 6630 0.0 34.45774 6 GAGTACA 11950 0.0 33.505512 1 AACGCAG 6840 0.0 33.39983 7 ATGGGGG 3455 0.0 33.233677 5 AGTACAT 11805 0.0 32.858727 2 GTCGGGA 575 0.0 31.040058 2 ATGGGAA 5925 0.0 31.02696 5 TATCAAC 7530 0.0 30.497303 3 >>END_MODULE