FastQCFastQC Report
Thu 26 May 2016
SRR936243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212016
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123730.5593540010560503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.41654309914575666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90970.4112538064824124No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.11329031978068874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70900.085.4550251
GTATCAA112850.053.49032
GTACATG186850.048.8140641
TACATGG192300.046.961822
ACATGGG191200.046.763913
TACCTGG19500.044.571462
CATGGGA143950.042.7176784
CATGGGG45550.041.427614
TCAACGC160350.038.905535
ATCAACG161200.038.700384
CAACGCA162950.038.4821856
ATGGGAT63650.037.596435
AACGCAG167300.037.5882877
TATCAAC169450.037.0277943
ATGGGAG50000.035.5975575
ACGCAGA193800.032.3871088
GTCGGGA5900.032.2876632
CGCAGAG196250.031.8008929
GAGTACA116100.031.7629851
AGTACAT114250.031.575852
Produced by FastQC (version 0.11.5)